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Article

Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling

by
Kibrom B. Abreha
1,*,†,
Erik Alexandersson
1,
Svante Resjö
1,
Åsa Lankinen
1,
Daniela Sueldo
2,‡,
Farnusch Kaschani
3,
Markus Kaiser
3,
Renier A. L. van der Hoorn
2,
Fredrik Levander
4,5 and
Erik Andreasson
1
1
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-234 22 Lomma, Sweden
2
Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
3
Chemische Biologie, Zentrum für Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-Essen, Universitätsstr. 2, 45117 Essen, Germany
4
Department of Immunotechnology, Lund University, SE-221 00 Lund, Sweden
5
National Bioinformatics Infrastructure Sweden (NBIS), Science for Life Laboratory, Lund University, SE-221 00 Lund, Sweden
*
Author to whom correspondence should be addressed.
†
Current address: Department of Plant Breeding, Swedish University of Agricultural Sciences, SE-234 22 Lomma, Sweden.
‡
Current address: Department of Biology, Norwegian University of Science and Technology, 5 Hogskoleringen, N-7491 Trondheim, Norway.
Int. J. Mol. Sci. 2021, 22(21), 12033; https://doi.org/10.3390/ijms222112033
Submission received: 30 September 2021 / Revised: 1 November 2021 / Accepted: 2 November 2021 / Published: 6 November 2021
(This article belongs to the Special Issue Plant Proteomic Research 4.0)
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Abstract

Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
Keywords: ABPP; apoplast; proteomics; serine hydrolases; β-glycosidases; potato; field-omics ABPP; apoplast; proteomics; serine hydrolases; β-glycosidases; potato; field-omics

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MDPI and ACS Style

Abreha, K.B.; Alexandersson, E.; Resjö, S.; Lankinen, Å.; Sueldo, D.; Kaschani, F.; Kaiser, M.; van der Hoorn, R.A.L.; Levander, F.; Andreasson, E. Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling. Int. J. Mol. Sci. 2021, 22, 12033. https://doi.org/10.3390/ijms222112033

AMA Style

Abreha KB, Alexandersson E, Resjö S, Lankinen Å, Sueldo D, Kaschani F, Kaiser M, van der Hoorn RAL, Levander F, Andreasson E. Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling. International Journal of Molecular Sciences. 2021; 22(21):12033. https://doi.org/10.3390/ijms222112033

Chicago/Turabian Style

Abreha, Kibrom B., Erik Alexandersson, Svante Resjö, Åsa Lankinen, Daniela Sueldo, Farnusch Kaschani, Markus Kaiser, Renier A. L. van der Hoorn, Fredrik Levander, and Erik Andreasson. 2021. "Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling" International Journal of Molecular Sciences 22, no. 21: 12033. https://doi.org/10.3390/ijms222112033

APA Style

Abreha, K. B., Alexandersson, E., Resjö, S., Lankinen, Å., Sueldo, D., Kaschani, F., Kaiser, M., van der Hoorn, R. A. L., Levander, F., & Andreasson, E. (2021). Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling. International Journal of Molecular Sciences, 22(21), 12033. https://doi.org/10.3390/ijms222112033

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