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Applications of Immunohistochemical Staining in Brain Diseases
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Department of Psychology, Fo Guang University, Yilan County 26247, Taiwan
Institute of Biomedical Sciences, Academia Sinica, Taipei 1529, Taiwan
Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam 13120, Republic of Korea
Department of Human Physiology and Pathology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Warszawska Av 30, 10-082 Olsztyn, Poland
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Applications of Immunohistochemical Staining in Brain Diseases

Abstract submission deadline
30 September 2024
Manuscript submission deadline
31 December 2024
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Topic Information

Dear Colleagues,

Immunohistochemical staining (IHC) can be used to qualitatively or quantitatively recognize analytes involving peptides, proteins, or small molecules. The IHC technique can link to molecular imaging, bioinformatics analysis, artificial intelligence (AI)-based methods, therapeutic targeting with brain therapy, and basic research in brain diseases. Advances in IHC-related applications enable early detection and precise treatment in disease management. This Topic aims to apply cutting-edge research into multidisciplinary frontier viewpoints to various novelty concerns and provide insights into clinical issues underlying recent advances in aspects of disease treatment. Authors are invited to submit both reviews and original articles to our Topic collection. Potential subject include, but are not limited to:

  1. Biomarkers or risk factors involving neuroinflammation or mitochondrial function in cell-to-animal innovative research of disease;
  2. Diagnostic strategies targeting various brain diseases;
  3. Molecular imaging that can reflect or predict disease development;
  4. Bench-to-bedside applications to disease diagnosis, treatment, and clinical outcome, including IHC-based molecular or pharmacological interventions;
  5. Biocybernetics and big data processing of large databases and genomics interactions using bioinformatics approaches;
  6. Label the biomarkers for targeting brain diseases.

Prof. Dr. Andrew Chih Wei Huang
Dr. Bai Chuang Shyu
Prof. Dr. Seong Soo A. An
Dr. Anna Kozłowska
Topic Editors

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Brain Sciences
brainsci 		3.3 3.9 2011 15.6 Days CHF 2200 Submit
Cells
cells 		6.0 9.0 2012 16.6 Days CHF 2700 Submit
Diagnostics
diagnostics 		3.6 3.6 2011 20.7 Days CHF 2600 Submit
International Journal of Molecular Sciences
ijms 		5.6 7.8 2000 16.3 Days CHF 2900 Submit
Journal of Personalized Medicine
jpm 		3.4 2.6 2011 17.8 Days CHF 2600 Submit

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Published Papers (1 paper)

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Article
A Scalable Histological Method to Embed and Section Multiple Brains Simultaneously
by Divine C. Nwafor, Stanley A. Benkovic, Briana L. Clary, Allison L. Brichacek, H. Wayne Lambert, Matthew J. Zdilla and Candice M. Brown
Cells 2024, 13(10), 860; https://doi.org/10.3390/cells13100860 - 17 May 2024
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Abstract
The preparation and processing of rodent brains for evaluation by immunohistochemistry is time-consuming. A large number of mouse brains are routinely used in experiments in neuroscience laboratories to evaluate several models of human diseases. Thus, methods are needed to reduce the time associated [...] Read more.
The preparation and processing of rodent brains for evaluation by immunohistochemistry is time-consuming. A large number of mouse brains are routinely used in experiments in neuroscience laboratories to evaluate several models of human diseases. Thus, methods are needed to reduce the time associated with processing brains for histology. A scalable method was developed to embed, section, and stain multiple mouse brains using supplies found in any common histology laboratory. Section collection schemes can be scaled to provide identical bregma locations between adjacent sections for immunohistochemistry, facilitating comprehensive, high-quality immunohistochemistry. As a result, sectioning and staining times are considerably reduced as sections from multiple blocks are stained simultaneously. This method improves on previous procedures and allows multiple embedding and subsequent immunostaining of brains easily with a dramatically reduced time requirement. Furthermore, we expand this method for use in numerous mouse tissues, rat brain tissue, and post-mortem human brain and arterial tissues. In summary, this procedure allows the processing of many rodent or human tissues from perfusion through microscopy in 10 days or less. Full article
(This article belongs to the Topic Applications of Immunohistochemical Staining in Brain Diseases)
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