Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii
Abstract
:1. Introduction
2. Results
2.1. Triterpenoid Compound Accumulation in R. roxburghii
2.2. Comparative Analysis of Differentially Expressed Genes
2.3. Genes Involved in Vitamin C, Sesquiterpenoid and Triterpenoid Biosynthesis Were Significantly Up-Regulated in Fruit of R. roxburghii
2.4. CYP and UGT Are Potentially Involved in Kajiichigoside Synthesis in R. roxburghii
2.5. Expression Pattern with Cluster 12 Is Involved in Kajiichigoside Synthesis in R. roxburghii
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. HPLC Analysis and Triterpenoid Content Determination
4.2.1. Extraction Process
4.2.2. Sample Preparation
4.2.3. Determination of Triterpenoid Content in Different Parts of R. roxburghii
4.2.4. Methodological Investigation
4.3. RNA Extraction and Library Construction
4.4. Transcriptome Data Processing and Alignment
4.5. Differential Expression Analysis and Functional Annotation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tissue | Kaji-Ichigoside F1 | Rosamultin | Euscaphic Acid | 2α,19α-Dihydroxyursolic Acid |
---|---|---|---|---|
Fruit | 1.29 ± 0.02 | 0.14 ± 0.01 | 0.62 ± 0.02 | 0.72 ± 0.01 |
Stem | 0.34 ± 0.01 | 0.48 ± 0.02 | 0.22 ± 0.02 | 0.36 ± 0.03 |
Root | 0.76 ± 0.03 | 0.02 ± 0.01 | 0.38 ± 0.03 | 0.33 ± 0.02 |
Leaf | 0.51 ± 0.03 | 0.03 ± 0.01 | 0.27 ± 0.01 | 0.31 ± 0.01 |
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Li, L.; Peng, M.; Yan, Y.; Deng, T.; Liang, Q.; Tao, X.; Li, H.; Yang, J.; He, G.; Yang, S.; et al. Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii. Int. J. Mol. Sci. 2024, 25, 5517. https://doi.org/10.3390/ijms25105517
Li L, Peng M, Yan Y, Deng T, Liang Q, Tao X, Li H, Yang J, He G, Yang S, et al. Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii. International Journal of Molecular Sciences. 2024; 25(10):5517. https://doi.org/10.3390/ijms25105517
Chicago/Turabian StyleLi, Liangqun, Mei Peng, Yanfang Yan, Tingfei Deng, Qiancheng Liang, Xian Tao, Haodong Li, Juan Yang, Guandi He, Sanwei Yang, and et al. 2024. "Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii" International Journal of Molecular Sciences 25, no. 10: 5517. https://doi.org/10.3390/ijms25105517