Green Approach in Obtaining Grape Extracts Displaying Cytotoxic Activity †
by
and
Nevena Dabetic
1,*, 
Vanja Todorovic
1,
Jelena Antic Stankovic
2,
Dragana Bozic
2,
Ivana Djuricic
1 and
Sladjana Sobajic
1 1
Department of Bromatology, Faculty of Pharmacy, University of Belgrade, 11129 Belgrade, Serbia
2
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, 11129 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
†
Presented at the 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023.
Proceedings 2023, 91(1), 227; https://doi.org/10.3390/proceedings2023091227
Published: 4 February 2024
(This article belongs to the Proceedings of The 14th European Nutrition Conference FENS 2023)
Abstract
:Background and objectives: Plant material is a real treasure of compounds possessing a spectrum of bioactivities. Their extraction is usually performed using toxic organic solvents, which causes serious environmental problems. For this reason, there is an aspiration to replace widely used organic solvents with greener ones. Natural deep eutectic solvents (NADES) were suggested at the beginning of this century as non-toxic, non-flammable and ecologically safe alternatives. Thus, the aim of the present study was to investigate the extraction efficiency of choline chloride (Ch)-based NADES (Ch with citric acid, molar ratio of 2:1, 30% of water, ChCit) in obtaining valuable extracts from grape skin. Materials and Methods: Eight different red grape varieties were collected. Skin separation was performed manually. Extraction was performed in an ultrasound bath under defined conditions (plant material/solvent ratio—1:10; time—30 min; temperature—50 °C). In parallel, acidified ethanol (EtOH) was used as a positive control. The total phenolic content (TPC) and the antioxidant and cytotoxic activities of the prepared extracts were explored. TPC investigations were performed using the Folin–Ciocalteu method. For antioxidant activity evaluation, four different in vitro assays were employed (DPPH, FRAP, ABTS and CUPRAC). Cytotoxic properties were tested against three cell lines (MRS-5, HeLa and LS 174T). Results and Discussion: TPC had a wide range (10.10–45.10 and 17.69–62.34 mg GAE/, for ethanol and ChCit, respectively), suggesting the strong influence of grape variety. ChCit showed higher efficiency in extracting polyphenols from grape skin compared with EtOH, probably since la ow ChCit pH potentiates anthocyanin extraction. Antioxidant activities displayed a strong correlation with TPC values (p < 0.05), confirming that phenols strongly contribute to the antioxidant properties of grape skin. The results for cytotoxicity were more heterogeneous. ChCit extracts inhibited cell growth (IC50 between 70.4 and 400 µg/mL), while EtOH extracts had no effect for the tested concentrations. There is no clear relationship between the polyphenol content and cytotoxic effects. Conclusion: Considering all of the mentioned result, it is concluded that the synergy between induced cell acidity and the present bioactives resulted in a cytotoxic effect of grape skin, but there is still a need to identify the specific compounds responsible for this.
Author Contributions
Conceptualization, N.D. and V.T.; methodology, V.T., J.A.S. and D.B.; investigation, N.D., V.T., J.A.S. and D.B.; writing—original draft preparation, N.D.; writing—review and editing, V.T.; visualization, I.D. and S.S.; supervision, S.S. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by Serbian Ministry of Education, Science, and Technological Development (451-03-68/2020-14/200161).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors on request.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Dabetic, N.; Todorovic, V.; Stankovic, J.A.; Bozic, D.; Djuricic, I.; Sobajic, S. Green Approach in Obtaining Grape Extracts Displaying Cytotoxic Activity. Proceedings 2023, 91, 227. https://doi.org/10.3390/proceedings2023091227
AMA Style
Dabetic N, Todorovic V, Stankovic JA, Bozic D, Djuricic I, Sobajic S. Green Approach in Obtaining Grape Extracts Displaying Cytotoxic Activity. Proceedings. 2023; 91(1):227. https://doi.org/10.3390/proceedings2023091227
Chicago/Turabian StyleDabetic, Nevena, Vanja Todorovic, Jelena Antic Stankovic, Dragana Bozic, Ivana Djuricic, and Sladjana Sobajic. 2023. "Green Approach in Obtaining Grape Extracts Displaying Cytotoxic Activity" Proceedings 91, no. 1: 227. https://doi.org/10.3390/proceedings2023091227
