Effects of Long-Term Sunflower Oil vs. Linseed Oil Diets on Fatty Acids Phospholipids and Desaturases in Hepatocytes †
by
, , and
Slavica Rankovic
1,*, 
Tamara Popovic
1,
Aleksandra Nenadovic
2,
Anica Stankovic
2,
Jasmina Debeljak Martacic
1,
Andjelija Ilic
3
and
Alexander Trbovich
2 1
Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia
2
School of Medicine, University of Belgrade, 11000 Belgrade, Serbia
3
Institute of Physics Belgrade, 11080 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
†
Presented at the 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023.
Proceedings 2023, 91(1), 172; https://doi.org/10.3390/proceedings2023091172
Published: 1 February 2024
(This article belongs to the Proceedings of The 14th European Nutrition Conference FENS 2023)
Abstract
:Background and Objectives: The liver plays a central role in the biosynthesis and metabolism of fatty acids. The liver’s phospholipids fatty acids composition depends on the dietary intake of lipids and the efficiency of enzymatic activity in the liver. Our study aimed to simultaneously investigate the liver’s phospholipids fatty acids composition and desaturase activity in response to long-term linseed or sunflower oil diets. Methods: We used adult female C57/BL6 mice and randomly divided them into a control and two other groups treated with 25% linseed or sunflower oils in isocaloric diet conditions. Before treatment, we analyzed the fatty acid profiles in dietary oils and hepatocytes. After 100 days of oil diet, we analyzed the fatty acids composition in the liver through GC-chromatography. Results: Sunflower oil elevated total monounsaturated fatty acids (MUFA) due to the increase in palmitoleic, oleic, and vaccenic acids. Linseed oil elevated linolenic (ALA), eicosapentaenoic (EPA), and docosapentaenoic (DPA) acids and reduced arachidonic (AA) and docosatetraenoic (DTA) acids, reducing the n-6/n-3 ratio. The estimated activity of desaturase 9 was significantly elevated in the sunflower oil group. The estimated activity of desaturase 5 was the highest, while the estimated activity of desaturase 6 was the lowest in the mice treated with linseed oil. Discussion: We showed that long-term linseed or sunflower oil consumption affects the liver’s phospholipids fatty acids composition in different ways. Sunflower oil could have beneficial effects on the liver tissue due to the increase in the total MUFA. Based on this and other studies, we conclude that the metabolism of n-3 PUFAs after linseed oil consumption is not sex-specific in the C57/BL6 mice model.
Author Contributions
S.R., T.P., and J.D.M. have written the manuscript. S.R. and J.D.M. have conducted experiments. A.T., A.S. and A.I. have supervised the work. A.N. and T.P. have performed statistical analyses. All authors have been involved in interpreting the results, contributed to drafting the discussion, and approved the final version of the manuscript. All authors have read and agreed to the published version of the manuscript.
Funding
This study was supported by grants No. 175075, No. III 41019, No. III-45003 and 0000-0003-0333-2118 from the Ministry of Education, Science and Technological Development, Government of Serbia.
Institutional Review Board Statement
Experimental Animals Ethics Committee at the University of Belgrade, Faculty of Medicine (approval number 323-07-09403/2014-05/1, dated 13 November 2015). Additionally, we conducted all experiments following the ARRIVE guidelines and the National Research Council’s Guide for the Care and Use of Laboratory Animals.
Informed Consent Statement
Not applicable.
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors on request.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Rankovic, S.; Popovic, T.; Nenadovic, A.; Stankovic, A.; Debeljak Martacic, J.; Ilic, A.; Trbovich, A. Effects of Long-Term Sunflower Oil vs. Linseed Oil Diets on Fatty Acids Phospholipids and Desaturases in Hepatocytes. Proceedings 2023, 91, 172. https://doi.org/10.3390/proceedings2023091172
AMA Style
Rankovic S, Popovic T, Nenadovic A, Stankovic A, Debeljak Martacic J, Ilic A, Trbovich A. Effects of Long-Term Sunflower Oil vs. Linseed Oil Diets on Fatty Acids Phospholipids and Desaturases in Hepatocytes. Proceedings. 2023; 91(1):172. https://doi.org/10.3390/proceedings2023091172
Chicago/Turabian StyleRankovic, Slavica, Tamara Popovic, Aleksandra Nenadovic, Anica Stankovic, Jasmina Debeljak Martacic, Andjelija Ilic, and Alexander Trbovich. 2023. "Effects of Long-Term Sunflower Oil vs. Linseed Oil Diets on Fatty Acids Phospholipids and Desaturases in Hepatocytes" Proceedings 91, no. 1: 172. https://doi.org/10.3390/proceedings2023091172
