- Article
FTIR–Fluorescence Two-Dimensional Correlation Spectroscopy of Soil Water-Extractable Particle Fractions by Sequential Membrane Filtration
- Dmitry S. Volkov,
- Olga B. Rogova and
- Mikhail A. Proskurnin
- + 1 author
The distribution of water-soluble organic matter (or dissolved organic matter DOM) in narrow (nano-and micrometer) fractions of chernozem was studied by sequential filtration on track-etched membranes. Multimodal (IR and fluorescence) two-dimensional correlation (2D-COS) spectroscopy was used. Protocols for attenuated total reflectance (ATR) FTIR of DOM were proposed. ATR-FTIR 2D-COS provides a larger volume of information on characteristic bands compared to traditional FTIR, especially in C–H ranges (3000–2800 and 1450–1300 cm−1). The fluorescence excitation–emission matrix 2D-COS showed that the indexes and ratios of humic- to protein-like compounds are reproducible, and exhibit significant variation among size fractions, with maximum amounts of saturated humic-like compounds in the largest (2–10 μm) and finest fractions (0.01–0.03 μm), while medium fractions (0.05–1 μm) are dominated by fulvic acids and fresh organic matter. Heterospectral fluorescence–IR 2D-COS enhanced the accuracy of identification and assessment of DOM group composition and showed that C–H IR band intensities correlate with tyrosine-like EEM bands and biogenic fluorescence indexes, while carboxylic components have humate-like bands and humification fluorescence indexes. Element profiles in DOM fractions correlate with fluorescence indexes; humification indexes with P, S, Cr, Mg, Ca, Cu, and Zn; biogenic with Mg, P, Cr, Cd, K, S, and Ca.
13 February 2026



![Regions of the excitation–emission matrix that correspond to certain groups of compounds. Green frames correspond to previously used boundaries, A, B, C, and T, which were proposed previously [85,86] in light magenta frames, and to broader boundaries A*, B*, C*, and T* proposed in this study; fraction of 0.01–0.03 µm. Details of the regions are summed up in Table 2.](https://mdpi-res.com/cdn-cgi/image/w=470,h=317/https://mdpi-res.com/soilsystems/soilsystems-10-00031/article_deploy/html/images/soilsystems-10-00031-g001-550.jpg)




