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Dentistry Journal

Dentistry Journal is an international, peer-reviewed, open access journal on dentistry, published monthly online by MDPI.

Indexed in PubMed | Quartile Ranking JCR - Q1 (Dentistry, Oral Surgery and Medicine)

All Articles (2,253)

Background/Objectives: This study aimed to compare the biological effects of two amelogenin-derived peptides—the leucine-rich amelogenin peptide (LRAP) and a synthetic peptide (SP)—on human dental pulp stem cells (hDPSCs) and human bone marrow–derived mesenchymal stem cells (hBMSCs). The investigation focused on cell viability, osteogenic differentiation, mineralization, gene expression, and β-catenin expression. Methods: hDPSCs and hBMSCs were cultured in osteogenic medium and treated with LRAP and SP at 1, 5, 10, 50, and 100 ng/mL. Cytotoxicity was assessed by MTT assay, while osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity and Alizarin Red S staining. Gene expression of RUNX2, COL1A1, OCN, MEPE, and DMP1 was quantified by qPCR. β-catenin localization was analyzed by immunofluorescence. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test (p < 0.05). Results: Both peptides exhibited good biocompatibility with hBMSCs, while high concentrations (≥50 ng/mL) reduced hDPSC viability. In both cell types, LRAP and SP increased ALP activity and mineral deposition in a concentration-dependent manner, with the greatest effects at 10 ng/mL. LRAP significantly upregulated osteogenic (RUNX2, COL1A1, OCN) and odontogenic (MEPE, DMP1) gene expression in hDPSCs. Immunofluorescence revealed nuclear β-catenin translocation in hDPSCs and membrane-associated accumulation in hBMSCs, indicating activation of canonical and non-canonical pathways, respectively. Conclusions: LRAP and SP promote osteogenic differentiation through distinct cell-type-specific signaling mechanisms, highlighting their potential as biomimetic agents for mineralized tissue regeneration.

6 February 2026

Cytotoxicity of SP (a,c) and LRAP (b,d) peptides (1, 5, 10, 50, and 100 ng/mL) evaluated on hDPSCs and hBMSCs with MTT assay after 48 h (n = 3). * p &lt; 0.05, ** p &lt; 0.01, **** p &lt; 0.0001.

Objective: This study aims to evaluate the retentive forces and oral health-related quality of life of completely edentulous subjects wearing heat-cured, 3D-printed, and polyamide complete denture (CD) bases at different intervals. Subjects and Methods: For this crossover study, 45 CDs were constructed for 15 completely edentulous male subjects, and subjects were randomly allocated to 3 equal groups (n = 5/group, 3 CDs/subject). Each subject was randomized to receive one manufactured CD—either heat-cured, polyamide, or 3D-printed. After 3 months, subjects crossed over to the other set, with 4 weeks’ rest between each CD. The retentive force (primary outcome) was measured for each maxillary CD base at baseline, after the first and third months; however, the oral health-related quality of life (second outcome) was evaluated for each CD after the first and third months using the oral health impact profile in the completely edentulous patient (OHIP-EDENT) questionnaire. Results: There were significant differences in retention forces between the polyamide CD and the other two CDs (p < 0.05); however, no significant difference was observed between the heat-cured and 3D-printed CDs at different intervals (p > 0.05). After 3 months of follow-up, significant differences in oral health-related quality of life were observed between polyamide and both 3D-printed and heat-cured CDs (p < 0.05). Additionally, the comparison between heat-cured and 3D-printed CDs revealed no significant variation in the overall OHIP-EDENT scores (p > 0.05). Conclusions: The retention of polyamide bases was higher than that of heat-cured and 3D-printed CDs. Additionally, oral health-related quality of life with polyamide dentures was superior to that of 3D-printed and heat-cured CDs across all OHIP-EDENT measures, except for social disability. Both 3D-printed and heat-cured CD bases provide retention and patient satisfaction within acceptable clinical measures.

6 February 2026

Flow diagram showing the participant enrollment, with the number of participants randomized, allocated to each study set, and dropouts. n—number.

Background: Dental caries and poor oral hygiene remain major public health problems among school-aged children, particularly in low- and middle-income countries. Teachers play a strategic role in delivering sustainable school-based oral health education; however, their effectiveness depends on appropriate pedagogical training. Objective: This study aimed to evaluate the effectiveness of a multimedia-assisted microteaching intervention for elementary school teachers in improving students’ oral health knowledge, attitudes, practices, and oral hygiene status. Methods: A mixed-methods sequential explanatory design was employed. Quantitative data were collected from 582 students and their teachers across three groups: multimedia-enhanced microteaching, multimedia-only training, and a control group. Outcomes were assessed using Knowledge–Attitude–Practice (KAP) questionnaires, the Oral Hygiene Index–Simplified (OHI-S), and the Decayed, Missing, and Filled Teeth (DMFT) index before and after a two-month implementation period. Non-parametric statistical tests were applied. Qualitative data were obtained through focus group discussions with teachers and were analyzed thematically. Results: Students in the multimedia-enhanced microteaching group demonstrated greater improvements in KAP scores and OHI-S values compared with the multimedia-only and control groups (p < 0.05). Qualitative findings indicated increased teacher confidence, improved classroom engagement, and better integration of oral health education into daily lessons. Changes in DMFT values were interpreted descriptively due to the short follow-up period. Conclusions: Multimedia-assisted microteaching appears to be a promising approach for strengthening teacher-led oral health education and improving short-term behavioral and hygiene outcomes among elementary school children. Further longitudinal studies are needed to assess long-term clinical effects.

5 February 2026

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Background/Objectives: Root canal instrumentation has a crucial role in the success of endodontic treatment. However, management of curved root canals remains a challenge. This study aimed to compare the performance of four rotatory file systems, ProTaper Next, TruNatomy, ProTaper Ultimate and Race Evo, in terms of wear, transportation and centering capacity, in curved root canals. Methods: A total of 150 human tooth roots were selected, divided based on the degree of curvature, and then distributed into four experimental groups according to the rotary system used. Cone beam computed tomography images were obtained before and after instrumentation, and values were measured with ImageJ software. Results: Regarding root canal wear, the TruNatomy system displayed the lowest wear values, and the Race Evo system showed a tendency for greater wear. For transportation, TruNatomy and Race Evo had the lowest transportation, indicating a higher respect for the root canal’s original anatomy. For centering ability, Race Evo and ProTaper Ultimate displayed values closer to perfect centering compared to other systems. Conclusions: Overall, TruNatomy was confirmed as a more conservative system, Race Evo with a tendency for greater wear even though with a higher respect for root canal original anatomy. Race Evo and ProTaper Ultimate showed better centering ability.

5 February 2026

Illustration of the Schneider method of evaluation of root curvature (α angle formed between the line parallel to the canal long axis and the line from the apical foramen to the first line).

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Dental Education
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Dental Education

Editors: Jelena Dumancic, Božana Lončar Brzak
Promoting Inclusion Oral-Health
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Promoting Inclusion Oral-Health

Social Interventions to Reduce Oral Health Inequities
Editors: Ruth E. Freeman

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Dent. J. - ISSN 2304-6767