In Vitro Biological Properties of Cyclodextrin-Based Polymers: Interaction with Human Serum Albumin, Red Blood Cells and Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Synthesis of HPCD and MCD Polymers Linked by Succinic Anhydride or 1,6-Hexamethylene Diisocyanate
2.2.2. FTIR-Spectroscopy
2.2.3. UV-Spectroscopy
2.2.4. Fluorescence Spectroscopy
2.2.5. NMR-Spectroscopy
2.2.6. Circular Dichroism Spectroscopy
2.2.7. Dynamic Light Scattering (DLS)
2.2.8. Nanoparticle Tracking Analysis (NTA)
2.2.9. Atomic Force Microscope
2.2.10. The CDpols Stability Studies
2.2.11. Hemolysis Assay
2.2.12. In Vitro Studies
3. Results and Discussion
3.1. Physico-Chemical Properties of CD-Based Polymers
3.2. The Structure of CDpols
3.3. Interaction of HSA with CD-Based Polymers
3.4. Influence of CD-Based Polymers on Binding HSA with Drugs
3.5. Blood Compatibility of CD-Polymers
3.6. Antibacterial Activity of CD-Polymers
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CD Type | Linker | Abbreviation | ζ-Potential, mV |
---|---|---|---|
HPCD | succinic anhydride1, 6-hexamethylene diisocyanate | HPolS HPolH | –0.6 ± 0.1 13.8 ± 0.7 |
MCD | succinic anhydride1, 6-hexamethylene diisocyanate | MPolS MPolH | 2.4 ± 1.2 31.6 ± 0.4 |
α-Helix | β-Structures | Random Coil | |
---|---|---|---|
HSA | 62 ± 2 | 21 ± 1 | 17 ± 1 |
HSA + HpolH | 60 ± 2 | 23 ± 1 | 17 ± 1 |
HSA + HPolS | 59 ± 2 | 23± 1 | 18 ± 1 |
HSA + MPolH | 59 ± 2 | 22 ± 1 | 19 ± 1 |
HSA + MPolS | 59 ± 2 | 25 ± 1 | 16 ± 1 |
Concentration, mg/mL | Type of Polymers | ||||
---|---|---|---|---|---|
HPolH | MPolH | HPolS | MPolS | HPolH–HPolS | |
3.30 | 1.9 ± 0.2 | 11.0 ± 0.5 | 2.4 ± 0.3 | 7.9 ± 0.4 | 0.6 ± 0.1 |
1.70 | 1.2 ± 0.1 | 4.2 ± 0.2 | 0 | 0 | 0.3 ± 0.1 |
0.17 | 0 | 0 | 0 | 0 | 0 |
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Yakupova, L.R.; Skuredina, A.A.; Kopnova, T.Y.; Kudryashova, E.V. In Vitro Biological Properties of Cyclodextrin-Based Polymers: Interaction with Human Serum Albumin, Red Blood Cells and Bacteria. Polysaccharides 2023, 4, 343-357. https://doi.org/10.3390/polysaccharides4040020
Yakupova LR, Skuredina AA, Kopnova TY, Kudryashova EV. In Vitro Biological Properties of Cyclodextrin-Based Polymers: Interaction with Human Serum Albumin, Red Blood Cells and Bacteria. Polysaccharides. 2023; 4(4):343-357. https://doi.org/10.3390/polysaccharides4040020
Chicago/Turabian StyleYakupova, Linara R., Anna A. Skuredina, Tatina Yu. Kopnova, and Elena V. Kudryashova. 2023. "In Vitro Biological Properties of Cyclodextrin-Based Polymers: Interaction with Human Serum Albumin, Red Blood Cells and Bacteria" Polysaccharides 4, no. 4: 343-357. https://doi.org/10.3390/polysaccharides4040020