Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rice Cultivation
2.2. As Concentration Measurement
2.3. Dry Weight and Si Concentration Measurement
2.4. Soluble Protein Contents Measurement
2.5. Transcriptomic Analyses
2.6. Quantitative RT-PCR
2.7. Statistical Analyses
3. Results
3.1. Arsenic Concentration of Shoots and Roots
3.2. Silicon Concentration of Shoots and Roots
3.3. Dry Weight of Shoots and Roots
3.4. Soluble Protein Contents of Shoots and Roots
3.5. Transcriptome Analysis of Rice Roots
3.6. Expression of Some Genes in DU-OE Exposed to Si and As
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Protein Name | Primer Sequences | Amplicon (bp) |
---|---|---|---|
GSTU1-F | Probable glutathione S-transferase GSTU1 | GTGAGTTTGTTGTTACCGTTGA | 84 |
GSTU1-R | GGACTCGTCAGTGTTGTACATA | ||
Os01g0369700-F | Os09g0367700 protein | GTTCGGTGAGATTCCAGTACTG | 86 |
Os01g0369700-R | TGTACTTGCGAAAGATGTACCT | ||
LOC_Os03g17460-F | IN2-1 protein, putative, expressed | CACCTTGATCTGCATTTGTCAA | 89 |
LOC_Os03g17460-R | ATGGGCAAATGTTGACATGTAC | ||
GSTZ5-F | Protein IN2-1 homolog B | AAGATTGTCGCGATTGATCTTG | 95 |
GSTZ5-R | TGATTGTTGTGCTCAAGTGAAG | ||
Os10g0528300-F | Glutathione S-transferase | CAAGATCTTCGACGAGGAGAAG | 123 |
Os10g0528300-R | CTCATCTTAGCGAACTCGACC | ||
Os09g0367700-F | Os09g0367700 protein | TGGTTCCACGCCTACGAGA | 126 |
Os09g0367700-R | CTTCTCAGGATCAGTAAGCGTC | ||
Os10g0530500-F | Os10g0530500 protein | ACAACATGTTCCCTGGAATGG | 166 |
Os10g0530500-R | TCGACGTACCCGATGGAGTC | ||
Os02g0685200-F | Os02g0685200 protein | CGGTGGGTTCTCGAATAACTC | 193 |
Os02g0685200-R | CGTGGTTGCAATTGACATCTTA | ||
CGA1-F | CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR 1 | CACAGAACCCGATATCCAAGG | 197 |
CGA1-R | GCCCTCATCAAATTAACGGTAC | ||
Os05g0573200-F | Isocitrate dehydrogenase [NADP] | CTAAATGGCACTGTGTTCAGAG | 252 |
Os05g0573200-R | GGACTCGTCAGTGTTGTACATA | ||
Os08g0522400-F | Os08g0522400 protein | CAAAGACAAGCTTTCACCGTAA | 282 |
Os08g0522400-R | CAGAAAAGAACGCTGCCTTTAA | ||
actin1(Os03g0718100)-F | CTTCATAGGAATGGAAGCTGCGGGTA | 26 | |
actin1(Os03g0718100)-R | CGACCACCTTGATCTTCATGCTGCTA |
Sampling Time | Parameter | Genotypes | As | Si | Genotypes × As | Genotypes × Si | As × Si | Genotypes × As × Si |
---|---|---|---|---|---|---|---|---|
1st week | Shoot As concentration | 42.01 ** | 16595.59 ** | 2453.05 ** | 118.54 ** | 50.85 ** | 904.34 ** | 15.45 ** |
Root As concentration | 22.18 ** | 10391.57 ** | 711.33 ** | 5.58 ** | 46.15 ** | 207.94 ** | 92.80 ** | |
Shoot dry weight | 17.21 ** | 55.04 ** | 22.71 ** | 0.70 | 1.42 | 2.11 | 0.26 | |
Root dry weight | 3.21 | 43.13 ** | 23.79 ** | 0.02 | 0.38 | 1.24 | 0.13 | |
2nd week | Shoot As concentration | 863.84 ** | 12289.71 ** | 2397.37 ** | 611.74 ** | 799.68 ** | 1161.99 ** | 371.13 ** |
Root As concentration | 32.77 ** | 12514.50 ** | 866.25 ** | 12.56 ** | 148.88 ** | 217.80 ** | 123.61 ** | |
Shoot dry weight | 2.87 ** | 45.87 ** | 28.03 ** | 0.06 | 0.25 | 1.20 | 0.30 | |
Root dry weight | 0.86 | 44.26 ** | 26.90 ** | 0.02 | 0.06 | 1.66 | 0.03 |
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Boorboori, M.R.; Li, Z.; Yan, X.; Dan, M.; Zhang, Z.; Lin, W.; Fang, C. Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes. Plants 2021, 10, 2210. https://doi.org/10.3390/plants10102210
Boorboori MR, Li Z, Yan X, Dan M, Zhang Z, Lin W, Fang C. Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes. Plants. 2021; 10(10):2210. https://doi.org/10.3390/plants10102210
Chicago/Turabian StyleBoorboori, Mohammad Reza, Zhou Li, Xue Yan, Mu Dan, Zhixing Zhang, Wenxiong Lin, and Changxun Fang. 2021. "Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes" Plants 10, no. 10: 2210. https://doi.org/10.3390/plants10102210