Prospection of Nematotoxic Aqueous Seeds Extracts Derived from the Preserved Arachis (Fabaceae) Germplasm Bank
Abstract
:1. Introduction
2. Materials and Methods
2.1. Obtaining Eggs and Second Stage Juveniles (J2) of Meloidogyne incognita from Infected Nicotiana tabacum Plants
2.2. Plant Extracts
2.3. Viability and Recovery Bioassay: Determine Nematicidal and/or Nematostatic Activity
2.4. Determination of the Lowest Lethal Dose
2.5. Estimate of Arachis ACEs Thermostability
2.6. Hemolysis Assay
3. Results and Discussion
3.1. Nematotoxic In Vitro Bioassays to Evaluate the Activity of ACEs from Arachis Plant Seeds against J2 of M. incognita
3.1.1. Viability In Vitro Bioassay
3.1.2. Recovery In Vitro Bioassay
3.2. Determination of the Lowest Lethal Dose of Arachis ACEs on J2 of M. incognita
3.3. Thermostability Test: Certifying the Thermal Stability of Nematotoxic Arachis AM1 ACE against J2 of M. incognita
3.4. Cytotoxicity: Evaluating the Cytotoxicity of Nematotoxic Arachis AM1 ACE against Bovine Red Cells (Female 3379)
3.5. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Species Name | Code BRA | Access Number |
---|---|---|
Arachis Stenosperma | AM1 | V 10309 |
Arachis triseminata | AM2 | V 13080 |
Arachis retusa | AM4 | V 12939 |
Arachis hassleri | AM5 | Sv 3818 |
Arachis appressipila | AM9 | V 9060 |
Arachis douradiana | AM16 | V 14682 |
Arachis archeri | AM17 | V 7614 |
Arachis gregoryi | AM18 | V 14957 |
Arachis duranensis | AM19 | V 14167 |
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Nascimento, B.; Brauna, C.; Ferreira, P.; Melo, L.; Ferreira, P.; Rocha, T. Prospection of Nematotoxic Aqueous Seeds Extracts Derived from the Preserved Arachis (Fabaceae) Germplasm Bank. Int. J. Plant Biol. 2024, 15, 1-12. https://doi.org/10.3390/ijpb15010001
Nascimento B, Brauna C, Ferreira P, Melo L, Ferreira P, Rocha T. Prospection of Nematotoxic Aqueous Seeds Extracts Derived from the Preserved Arachis (Fabaceae) Germplasm Bank. International Journal of Plant Biology. 2024; 15(1):1-12. https://doi.org/10.3390/ijpb15010001
Chicago/Turabian StyleNascimento, Bruna, Cristiane Brauna, Paula Ferreira, Luis Melo, Paulo Ferreira, and Thales Rocha. 2024. "Prospection of Nematotoxic Aqueous Seeds Extracts Derived from the Preserved Arachis (Fabaceae) Germplasm Bank" International Journal of Plant Biology 15, no. 1: 1-12. https://doi.org/10.3390/ijpb15010001