Search Results (79)

The Moroccan Ryad, a cherished architectural treasure in North Africa, symbolizes cultural richness and architectural legacy. This paper advocates an inventive strategy to preserve and rejuvenate the Ryad as an energy-efficient model, intertwining modern sustainability principles. Employing a multidisciplinary approach, this study delves into the fusion of traditional Ryad design with contemporary sustainability, tackling energy efficiency, resource conservation, and cultural heritage challenges. Examining historical construction methods, material choices, and spatial arrangements alongside sustainable building practices, this paper showcases case studies of newly constructed sustainable Ryads. These examples spotlight strategies like passive solar design, renewable energy incorporation, water conservation, and local material use, augmenting energy performance while fostering cultural continuity through eco-friendly materials and traditional craftsmanship. This research also explores the social and economic impacts of this sustainable Ryad model, including its potential to boost tourism, generate employment, and engage communities. Additionally, it addresses the role of public policy in supporting sustainable practices for preserving Moroccan cultural heritage. By amalgamating culture, sustainability, and energy efficiency, this paper envisions a new role for the Moroccan Ryad, positioning it not only as a guardian of heritage but also as a symbol of environmentally responsible architecture—a model applicable to culturally rich regions globally. Full article

Exosomes, naturally derived extracellular vesicles, have emerged as powerful bio-nanocarriers in precision medicine. Their endogenous origin, biocompatibility, and ability to encapsulate and deliver diverse therapeutic payloads position them as transformative tools in drug delivery, gene therapy, and regenerative medicine. This review presents a comprehensive analysis of exosome-based therapeutics across multiple biomedical domains, including cancer, neurological and infectious diseases, immune modulation, and tissue repair. Exosomes derived from stem cells, immune cells, or engineered lines can be loaded with small molecules, RNA, or CRISPR-Cas systems, offering highly specific and low-immunogenic alternatives to viral vectors or synthetic nanoparticles. We explore endogenous and exogenous loading strategies, surface functionalization techniques for targeted delivery, and innovations that allow exosomes to traverse physiological barriers such as the blood–brain barrier. Furthermore, exosomes demonstrate immunomodulatory and regenerative properties in autoimmune and degenerative conditions, with promising roles in skin rejuvenation and cosmeceuticals. Despite their potential, challenges remain in large-scale production, cargo loading efficiency, and regulatory translation. Recent clinical trials and industry efforts underscore the accelerating momentum in this field. Exosomes represent a promising platform in precision medicine, though further standardization and validation are required before widespread clinical use. This review offers critical insights into current technologies, therapeutic mechanisms, and future directions to unlock the full translational potential of exosomes in clinical practice. Full article

Wharton’s Jelly-derived mesenchymal stem cell (WJ-MSC) secretome contains diverse bioactive factors with potential for skin regeneration, but its clinical efficacy is limited by poor transdermal delivery. In this study, we developed a dual-delivery system by nanoencapsulating WJ-MSC secretome and coating it onto marine sponge-derived spicules. Physicochemical characterization, in vitro assays (fibroblast and keratinocyte proliferation, keratinocyte migration, type I procollagen secretion, and antioxidant activity), and in vivo penetration studies were conducted. A single-arm clinical trial evaluated dermal absorption, pore characteristics, skin texture, wrinkles, and pigmentation following topical application. Transdermal penetration efficiency was significantly higher in the nano-coated spicule group than in the uncoated secretome control. In vitro, secretome treatment promoted fibroblast and keratinocyte activity, accelerated wound closure, and increased collagen synthesis. Clinically, a single application enhanced dermal absorption and significantly reduced pore number, while two weeks of treatment decreased wrinkles and pigmentation. Spicule-based nanoencapsulation effectively overcomes the skin barrier, enhances the regenerative activity of WJ-MSC secretome, and induces measurable clinical improvements in skin rejuvenation. This platform represents a promising cosmetic and therapeutic strategy in dermatology. Full article

The durability of asphalt pavements is crucial for sustainable road infrastructures. This systematic review compares the Marshall and Superpave asphalt mix design protocols, with a particular focus on the integration of polymer-modified bitumen (PMB) and rejuvenators. Although the Marshall method remains widely used for its simplicity and cost-efficiency, its empirical basis limits its effectiveness to meet modern pavement performance demands. In contrast, the Superpave system offers improved resistance to rutting, longer fatigue life, and better mitigation of moisture damage. The review traces the evolution of asphalt mix design, identifies current challenges, and emphasizes the need for transitioning toward performance-based frameworks. Special attention is given to the incorporation of polymers such as Styrene–Butadiene–Styrene (SBS), Styrene–Butadiene–Rubber (SBR), and Polyethylene (PE), which significantly enhance the mechanical properties of asphalt mixtures. The role of rejuvenators in restoring aged binders and enabling pavement recycling is also examined. Finally, the manuscript provides strategic recommendations for adopting Superpave to enhance pavement durability and reduce lifecycle maintenance costs. Overall, this comprehensive review advances knowledge on asphalt mix design, fostering innovation and sustainability while promoting long-term resilience in road pavement infrastructures. Full article

Background: Reactive oxygen species (ROS) generated due to mitochondrial dysfunction are one of the primary causes of the initiation and progression of senescence. Although reducing mitochondrial ROS production is known as an effective strategy for the treatment of aging, effective components that reduce mitochondrial ROS production or effective treatments that utilize them have not yet been developed. Methods: Screening of plant-generated secondary metabolites to overcome ROS-mediated stress found that ε-viniferin, a dimer of resveratrol, effectively reduces mitochondrial ROS production. Results: ε-viniferin induced efficient electron transport and reduced mitochondrial ROS, a consequence of inefficient electron transport. In addition, ε-viniferin acted as a senolytic that selectively eliminates senescent fibroblasts, thereby restoring mitochondrial function and senescence-associated phenotypes. RNA sequencing analysis revealed that regulator of G protein signaling 16 (RGS16) was an important gene for ε-viniferin-mediated senescence rejuvenation. Upregulation of RGS16 showed similar effects as ε-viniferin in reducing mitochondrial ROS production and restoring mitochondrial function. Conclusions: This study discovered a novel mechanism by which ε-viniferin rejuvenates senescence by lowering ROS production in mitochondria. The novel mechanism will serve as a basis for developing therapeutics that regulate mitochondrial ROS production to treat aging. Full article

Background: Hyaluronic acid (HA) has emerged as a favored adjunct to restore volume after blepharoplasty and is very effective in the treatment of postoperative hollowness, sagging, and asymmetry. Its efficacy, rate of complications, and optimal injection technique are different in different clinical studies. Hyaluronidase has been studied by diverse methods in the treatment of HA complications, including chronic edema and surgical distortion. This study critically evaluated the efficacy, safety, and technical aspects of HA in the context of blepharoplasty outcomes. Methods: A systematic review was performed to evaluate the use of HA and hyaluronidase for post-blepharoplasty volume rejuvenation and the treatment of complications. Studies describing HA injection technique, time interval between blepharoplasty and injection, volumetric maintenance, complication rates, esthetic and functional results, and patient satisfaction scores were considered. Risk of bias was estimated with the ROBINS-I tool. Results: Sample sizes across the five included studies ranged from 5 to 109 patients, and follow-up intervals ranged from 1 month to 7 years. The age of patients ranged from 31 to 76 years, and females accounted for 86% of the participants in some studies. Injection of HA successfully restored meaningful volume, with retention persisting for over 12 months in the majority of cases. HA preoperative injection caused significant patient satisfaction in a short duration and was not associated with severe complications; delayed injection caused slight distortions in some revision operations. Lipofilling showed a reduced rate of complications (12%) compared with isolated blepharoplasty (20%), suggesting its utility as an adjuvant procedure of volume restoration. Hyaluronidase successfully treated recalcitrant edema, with improvements ranging from 50% to 100%, while the application of adjuvant RF microneedling caused complete remission (100%) in subjects with multiple treatments. The application of ultrasound imaging made measurements more precise, although methods of clinical assessment were significantly heterogeneous among the studies. Conclusions: HA displayed efficacy in terms of efficient volume restoration after blepharoplasty, especially when technique, time, and filler selection are meticulously optimized. In comparison to lipofilling, HA is seen as somewhat safer because of its reversibility and lower likelihood of adverse vascular events. Nonetheless, considerable variability in filler type, amount, timing of administration, and result evaluation constrains conclusive clinical recommendations. The use of hyaluronidase is an effective remedial approach for overcorrection or ongoing edema. Full article

The use of Reclaimed Asphalt Pavement (RAP) is a sustainable strategy to conserve natural resources, reduce environmental pollution, and lower construction costs. However, aged asphalt in RAP suffers from oxidation and loss of light fractions, increasing stiffness and brittleness. A key scientific challenge is how to effectively restore the performance of aged asphalt while maintaining cost efficiency. In this study, a novel asphalt rejuvenator was developed to address this issue. The rejuvenator consists of 6% aromatic oil-like materials to replenish light components, 1.52% plasticizer to enhance ductility, and 0.3% modifier A to improve adhesion, with a total dosage of 7.82% by the mass of the aged binder. The rejuvenator meets the requirements of Chinese specifications. The performance evaluation was conducted at both asphalt binder and mixture scales. The results show that the rejuvenator significantly improves low-temperature cracking resistance and medium-temperature fatigue performance of aged binders, although it slightly reduces high-temperature rutting resistance. When applied to asphalt mixtures with 45% RAP, the rejuvenated mixtures exhibited enhanced low-temperature performance. A comparative analysis with commercial rejuvenators confirmed the developed product’s competitive performance and economic benefit. This study provides technical insight into rejuvenator design and addresses critical challenges in RAP recycling for sustainable pavement engineering. Full article

Salak peel extract has various biological properties befitting cosmeceutical applications; however, their practical uses are still limited due to their low water solubility and stability. Encapsulation technology was employed to alleviate these issues. In this work, we presented a simple method to prepare ethosome-encapsulated salak peel extract using green solvents (ethanol and water). For this purpose, we used 95% ethanol to extract salak peel and explored its activities. Results showed that, in addition to anti-oxidant, the extract also showed anti-tyrosinase, anti-inflammatory, and anti-bacterial (against S. aureus) activities. These activities indicate its potential uses in cosmeceutical applications. We further encapsulated the extract in ethosomes using a stirrer and green solvents for the preparation methods. The yielded ethosomes exhibited a size range of 120 to 205 nm, polydispersity index (PDI) of 0.15 to 0.25, and zeta potential of −35 to −60 mV depending on the amount of L-α-phosphatidylcholine used. The highest encapsulation efficiency was approximately 30%. The antiradical capacity and anti-inflammatory activities of salak peel extract were also found to be maintained after the encapsulation process. An in vitro biocompatibility study of the extract after encapsulation was also performed. The results not only indicated good biocompatibility, but also the potential skin-rejuvenating ability of salak peel ethosomes. A stability study was also performed, and the results suggested that these ethosomes were stable at different conditions. With further investigation, salak peel ethosomes, as presented here, can be suitable for cosmeceutical applications. Full article

The cement industry faces increasing energy costs and environmental pressures, driving the adoption of alternative fuels derived from waste materials. In Togo, approximately 350,000 t of end-of-life tires (ELT) are generated annually, creating significant environmental and health hazards through uncontrolled disposal and burning practices. This study investigated the technical feasibility and economic viability of incorporating waste tires as an alternative fuel in cement manufacturing. Tire-derived fuel (TDF) performance was evaluated by comparing pre-processed industrial tires with unprocessed ones, focusing on clinker production loss, elemental composition, heating values, and bulk density. The results demonstrate that TDF exhibits superior performance characteristics, with the highest heating values, and meets all the required specifications for cement production. In contrast, whole tire incineration fails to satisfy the recommended criteria, necessitating blending with conventional fuels to maintain clinker quality and combustion efficiency. The investigation revealed no significant adverse effects on production processes or clinker quality while achieving substantial reductions in nitrogen and sulfur oxide emissions. The experimental results were compared with the theoretical burnout times to optimize the shredding operations and injection methods. However, several challenges remain unaddressed, including the absence of streamlined handling processes, limited understanding of long-term ecological and health impacts, and insufficient techno-economic assessments. Future research should prioritize identifying critical aging points, investigating self-rejuvenating behaviors, and quantifying long-term environmental implications. These findings provide a foundation for developing computational models to optimize the mixing ratios of alternative and fossil fuels in cement manufacturing, offering significant environmental, economic, and societal benefits for the cement industry. Full article

Keratinocyte stress, caused by various intrinsic and extrinsic factors, contributes to the overall aging process. D-galactose-induced metabolic/oxidative stress is a commonly used in vitro model for studying premature aging. Due to their rich composition of bioactive molecules that influence critical pathways in cellular aging and rejuvenation, placental derivatives have a well-established history in anti-aging skincare and therapy. However, trophoblast-derived extracellular vesicle (TEV) effects on D-galactose-induced premature aging in keratinocytes have not been investigated yet. TEV pretreatment for 24 h enhanced cellular resilience against D-galactose-induced stress, judging by the downregulated expression of senescence- and stress-associated markers (p19 and p21, HIF-1α, mTOR), and reduced production of reactive oxygen species and DNA damage. Additionally, TEV pretreatment enhanced keratinocyte proliferation and integrin-β1 subunit expression upon D-galactose exposure, most likely contributing to more efficient wound closure. In conclusion, this study underscores the potential of TEVs to modify expression of stress- and senescence-related proteins in keratinocytes and improve their wound healing properties. Their regenerative and protective characteristics position TEVs as promising candidates for developing innovative procedures to address skin conditions related to premature aging. Full article

In plants, the transition from the juvenile to adult stage involves physiological and anatomical changes initiated and partially controlled by evolutionarily conserved microRNAs. This process is of particular significance for the successful propagation of woody plant species that have transitioned to vegetative maturity and are recalcitrant to propagation. Conserved miRNAs differentially expressed between rejuvenated and mature Ilex paraguariensis plants were identified using high-throughput sequencing of small RNA libraries. The expression of miR156/miR157/miR528 was high in the leaves of juvenile plants and gradually decreased as the plant transitioned from juvenile to adult stages. In contrast, miR172 was predominantly expressed in adult plants. This variation confirmed that adults transitioned back to a juvenile phase after serial-rooted cuttings, allowing the plants to regain juvenile characteristics. Rejuvenation promotes the formation of adventitious roots and improves root structure, which supports the overall growth of the plant and results in greater vigour. The results will offer insights for further investigation into the molecular mechanisms regulating vegetative phase change in I. paraguariensis and other recalcitrant woody plant species. This knowledge could facilitate the earlier identification of rejuvenated material by analysing a wider range of genotypes and maturation stages, enhancing the efficiency of Ilex paraguariensis mass propagation. Full article

Hippophae rhamnoides (family Elaeagnaceae) is a deciduous shrub that has become a uniquely advantageous species in the arsenic sandstone area of Inner Mongolia due to its well-developed root system and strong tillering ability. This study, by taking 10-year-old H. rhamnoides in the arsenic sandstone area as the research object and analyzing the morphological traits of their fine roots and their coordination within soil under different stubble heights (0, 10, 15, and 20 cm) and non-stubble treatment, aims to select the optimal stubble height that is most conducive to the rejuvenation of H. rhamnoides and thus improve the decline in the productivity of H. rhamnoides in this region. The results reveal significant differences in fine root and soil properties under different stubble heights (p < 0.05). Among different traits, fine root area density shows the highest total coefficient of variation, making it the most sensitive trait. Principal component analysis results indicate that after stubble treatment, the traits of H. rhamnoides fine roots center on high specific surface area (0.316) + high specific root length (0.312), shifting toward a resource-acquisition ecological strategy with the best foraging efficiency observed under a stubble height of 15 cm. Soil N:P and C:P can explain 66% and 61% of the root morphological traits strategies deployed during stubble treatment, respectively. Fine roots exhibit high adaptability to the breaking of phosphorus limitation and fixation of carbon and nitrogen. Full article

As a polymer-like organic material, asphalt often undergoes aging during service life. Regeneration technology is the main approach to achieve its recycling; therefore, the rejuvenator is an important factor affecting the regeneration effect. In order to evaluate the rejuvenation effects of rejuvenators on aged asphalt, fluid catalytic cracking (FCC) slurry and a penetrant containing epoxy functional groups were used to prepare conventional rejuvenators (CR1 and CR2) and a penetrable rejuvenator (PR). The impact of the penetrant on the physical properties of the rejuvenator was investigated, and the rejuvenation effects of different rejuvenators on mild and severe aged asphalt were evaluated through physical and rheological tests. Results show that the penetrant effectively lubricates the movement of rejuvenator molecules, improving the high temperature stability and aging resistance of the rejuvenator. CR1 and CR2 are more suitable for mild aged asphalt, as mild aging has a relatively minimal effect on the chemical composition and colloidal structure of asphalt. At a 25% dosage, the PR significantly restores the physical properties of severe aged asphalt, while CR1 and CR2 still fail to meet specifications. The PR is more effective for severe aged asphalt because it not only dissolves and disperses asphaltenes but also weakens interactions between asphaltenes, which facilitates a more effective restoration of the colloidal structure and significantly enhances the rejuvenation effect. The findings of this study provide insights into the design of penetrable rejuvenators for a more efficient utilization of reclaimed asphalt pavement (RAP). Full article

One of the major causes of senescence is oxidative stress caused by ROS, which is mainly generated from dysfunctional mitochondria. Strategies to limit mitochondrial ROS production are considered important for reversing senescence, but effective approaches to reduce them have not yet been developed. In this study, we screened the secondary metabolites that plants produce under oxidative stress and discovered sauchinone as a potential candidate. Sauchinone induced mitochondrial function recovery, enabling efficient electron transport within the electron transport chain (ETC). This led to a decrease in ROS production, a byproduct of inefficient electron transport. The reduction in ROS by sauchinone rejuvenated senescence-associated phenotypes. To understand the underlying mechanism by which sauchinone rejuvenates senescence, we carried out RNA sequencing and found VAMP8 as a key gene. VAMP8 was downregulated by sauchinone. Knockdown of VAMP8 decreased mitochondrial ROS levels and subsequently rejuvenated mitochondrial function, which was similar to the effect of sauchinone. Taken together, these studies revealed a novel mechanism by which sauchinone reduces mitochondrial ROS production by regulating mitochondrial function and VAMP8 expression. Our results open a new avenue for aging research to control senescence by regulating mitochondrial ROS production. Full article

In recent years, the employment of rejuvenators and warm mix asphalt (WMA) additives for reclaimed asphalt pavement (RAP) has been recognized as a popular approach to increase the recycling rate of waste materials and promote the sustainable development of pavement engineering. However, the composition of warm mix recycled asphalt binder is complicated, and the microstructural changes brought about by the rejuvenators and WMA additives are critical in determining its macroscopic mechanical properties. This research focuses on the atomic modeling of the rejuvenators and WMA additives diffusion behavior of the warm mix recycled asphalt binder. The objective is to reveal the thermodynamic performance and diffusion mechanism of the WMA binder under the dual presence of rejuvenators and WMA additives. Three types of mutual diffusion systems (Aged and oil + virgin + wax, Aged + virgin + wax, and Aged and oil + virgin) were established, respectively, for a comparative investigation of the glass transition temperature, viscosity, thermodynamics, free volume, and diffusion behavior. The results indicate a 44.27% and 31.33% decrease in the glass transition temperature and apparent viscosity, respectively, after the incorporation of 5% oil rejuvenators in the Aged + virgin + wax asphalt binder, demonstrating the improved cracking resistance and construction workability. The presence of the RAP binder and organic WMA additives raised the cohesion of the asphalt binder and decreased self-healing ability and free volume, and these detrimental influences can be offset by the introduction of rejuvenators. The combined use of rejuvenators and organic WMA additives remarkably enhanced the de-agglomeration to asphaltenes, stimulated the activity of aged RAP macromolecular components, and ultimately improved the blending efficiency of virgin binders with the overall structure of RAP binders. Full article