Search Results (4,722)

Bean common mosaic virus (BCMV; Potyvirus phaseovulgaris) is one of the primary viruses that severely impacts the yield and quality of common beans (Phaseolus vulgaris L.) and has a worldwide distribution. Utilizing small RNA sequencing and RT-PCR validation, this study identified widespread co-infection by multiple viruses in field-collected common bean samples, with BCMV being the dominant viral species. A novel isolate, designated DY9, was obtained from these field samples. Pathotype characterization confirmed DY9 as pathotype PG-III, while previous studies reported all other PG-III members as Bean common mosaic necrosis virus (BCMNV). Whole-genome sequencing and phylogenetic analysis revealed that DY9 was genetically closer to BCMV and diverged significantly from known PG-III isolates. Based on these findings, we constructed an infectious clone of DY9. To address the genetic instability of Potyvirus in the Escherichia coli (E. coli) expression system, we discovered that inserting Intron 2 (derived from the NiR gene of P. vulgaris, GenBank: U10419.1) at position 2431 of the HC-Pro gene and targeting Intron 1 (derived from the ST LS1 gene of Solanum tuberosum, GenBank: X04753.1) at position 4240 of the CI gene significantly improved the stability of the cloning vector. The clone was verified to systemically infect common bean plants and induce typical mosaic symptoms. Infectivity was validated through RT-PCR, RT-qPCR, Western blotting, and transmission electron microscopy. This study represents the first successful construction of an infectious clone for pathotype PG-III BCMV, providing a critical reverse genetics tool for dissecting viral pathogenesis and identifying resistance genes. These findings not only expand the genetic diversity of BCMV but also offer a methodological reference for constructing infectious clones of Potyvirus species. Full article

The entry is intended to define the concepts of risk communication and emergency communication. At the same time, it explains the difference not only from a communication point of view but also from a cultural one. Risk and emergency are two sociologically relevant events, and they are culturally constructed. They are events that bring about a socio-cultural change, which, in turn, is triggered by the population’s responses on the basis of the social perception of the events themselves, also conveyed by the different forms of communication. When communicating risk and emergencies, it is essential to educate people about alert and emergency systems. Above all, what they refer to and what kind of message they contain. The “warning communication” must be specific and refer exclusively to the threat to start the first phase of the communication through which it is possible to understand the type of threat and define the communication plan to be implemented later. The use of social media, which is strongly spread in digital society, allows not only rapid dissemination of information but also rapid communication and message selection (speed and content of the message are equally important). Alert and warning systems are very often linked to risk systems, since the risk from natural disasters (eruptions, earthquakes, tsunamis) or technological catastrophes (nuclear power plant explosions) follows emergency phases when the phenomenon occurs. The communication processes, in and emergency, must be able to explain, persuade but also confer an assist the political decision-maker and the decision-making process itself through an alert system (especially in the first phase), followed by continuous dissemination through the media that the digital society offers, as well as through the usual systems adopted by government bodies (for example, bulletins and news), specialized research institutions and institutes with information and communication functions. In risk and emergency management, information and communication are to be considered, respectively, a basic element and a means of dissemination and training to educate the population to perceive a risk, to recognise emergencies and the possible impact of the risk. Differences will be expressed and analysed with reference to international examples. Full article

The growing number of patients after oncological treatment makes knowledge about potential cardiovascular complications of cancer therapy particularly important. Early recognition of symptoms enables the rapid initiation of appropriate therapy and improves outcomes. Education in this field increases awareness of the need for regular cardiology follow-up and adherence to health recommendations. It is advisable for patient education on the risk of cardiotoxicity to be included during visits with both the oncologist and the cardiologist. A self-developed questionnaire was used. It consisted of 40 questions (including 16 from the Health Behavior Scale) and 8 additional sociodemographic questions. An anonymous questionnaire was completed by 243 patients of the cardio-oncology outpatient clinic operating within the Department of Cardiology in Poland. In the survey conducted, patients were asked to define the concept of cardio-oncology; only 23.5% of respondents provided a correct answer. The highest level of awareness was observed among individuals under the age of 40 (p = 0.001) and of higher education levels (p < 0.001). Better knowledge was also noted among respondents who recalled being informed by their doctor about complications (p < 0.001) and among those who had undergone cardiological examinations (p = 0.005). The findings further revealed that respondents who recognized the importance of cardiac monitoring following therapy were significantly more likely to engage in health behaviors (p < 0.001). Particularly concerning was the limited communication regarding cardiovascular risks associated with cancer treatment. Only 24.3% of patients reported having been informed (or recalled being informed) by their oncologist about the potential cardiotoxic effects of anticancer drugs. Approximately one-third of respondents (32%) had not been referred for a cardiology consultation during their cancer treatment. Despite this, an overwhelming majority (95.5%) expressed the belief that a cardiologist should assess all oncology patients. These findings underscore critical deficiencies in patients’ education within the field of cardio-oncology. Health education interventions during oncological follow-up visits are needed Full article

Canine prostatic carcinoma is a highly aggressive neoplasm with a strong tendency to metastasize, most commonly to regional lymph nodes, lungs, and bones. However, skeletal muscle and myocardial involvement are rarely reported. This report describes an 11-year-old intact male Maltese dog with a two-month history of anorexia and lethargy, referred for further evaluation after failing to respond to piroxicam therapy. Diagnostic imaging revealed a prostatic mass and multiple rim-enhancing lesions in the epaxial musculature and left ventricular wall, without evidence of metastasis to the lymph nodes, lungs, or other visceral organs. Hemostatic analysis indicated a hypercoagulable state. Postmortem examination confirmed metastatic prostatic carcinoma involving the semispinalis, multifidus, and myocardium. Histologically, the neoplastic cells exhibited similar morphology at the primary and metastatic sites. Immunohistochemistry revealed strong cytokeratin expression and absence of uroplakin III, consistent with a non-urothelial epithelial origin. No evidence of lymphatic involvement was observed. To the best of our knowledge, this is the first documented case of canine prostatic carcinoma with exclusive myotropic and myocardial metastases. These findings suggest a possible hematogenous metastatic route and highlight the importance of including muscle and cardiac tissues in staging protocols for canine prostatic carcinoma, even when lymphadenopathy or pulmonary lesions are absent. Full article

Background/Objectives: Juzentaihoto (JTT), a traditional Kampo formula composed of ten medicinal herbs, is widely prescribed in Japan for immune enhancement and general health maintenance. This exploratory, open-label pilot study aimed to evaluate the feasibility and immunomodulatory effects of JTT in cancer patients and to explore its potential mechanisms of action. Methods: Ten cancer patients received oral JTT (7.5 g/day) for 14 days, while healthy volunteers served as a reference group. Peripheral natural killer (NK) cell phenotypes and CD95 expression were analyzed by flow cytometry, and serum Fas ligand (FasL) concentrations were measured by ELISA. Complementary in vitro assays using PBS-extracted, autoclaved JTT were conducted to assess Fas/FasL-mediated apoptosis in Jurkat and primary T cells by flow cytometry and Western blotting for cleaved caspase-8 and -3. Additional experiments with staurosporine (intrinsic apoptosis) and TRAIL in OSC-19 carcinoma cells were performed to determine pathway specificity. Results: In patients, most NK-cell markers showed no statistically significant within-subject changes, although a trend-level increase in NKp46 and a significant reduction in NK-cell CD95 expression (paired p = 0.014) were observed. Between-group differences primarily reflected baseline disparities between cancer patients and healthy controls. In vitro, JTT (50–100 µg/mL) partially attenuated FasL-induced apoptosis and reduced cleaved caspase-3 without affecting cleaved caspase-8, suggesting selective downstream modulation of the extrinsic pathway. Conclusions: Within the limitations of a small, non-randomized cohort without placebo, these findings are hypothesis-generating and indicate that JTT selectively modulates Fas-mediated lymphocyte apoptosis without promoting tumor immune evasion. Further randomized trials and mechanistic studies incorporating co-culture or 3D tumor–immune models are warranted to confirm these observations and identify active constituents. Full article

Background/Objectives: The cell surface proteoglycan glypican-3 (GPC3) is reportedly overexpressed in hepatocellular carcinoma (HCC) tissues, but not in benign liver tissues, rendering this protein a potential target for radionuclide theranostic approaches. Peptides are generally a promising class of targeting molecules for the development of radioligands because they combine straightforward synthetic access with favorable pharmacokinetics. Among the published peptides with disclosed structures, one of the most promising radioligands is [¹⁸F]AlF-NOTA-TJ12P2, which has a reported comparably high binding affinity to GPC3 and a high hydrophilicity. In this study, we aimed to design novel GPC3-targeting radioligands based on the TJ12P2 peptidic scaffold. Methods: Peptides were synthesized on solid phase using an Fmoc protecting group strategy. For comparative investigations, the reference nanobody HN3 was expressed in E. coli, isolated and subsequently modified with NODA-GA or SulfoCy3. The binding of native peptides, scrambled variants and reference nanobodies to GPC3 was investigated by surface plasmon resonance (SPR) interaction analysis, and fluorescently labeled versions of peptides and nanobodies were used for fluorescence microscopy in HepG2 (GPC3+) or SK Hep1 (GPC3−) cells. The chelator-bearing peptides were radiolabeled with gallium-67 and their stability towards radiolysis and in human serum was investigated. The binding of radiolabeled peptides and nanobodies to HepG2 cells was assessed in real-time ligand binding experiments. Results: The synthesized native peptides did not exhibit binding towards GPC3 in SPR interaction analyses, and the observed response was comparable to that of the scrambled variants at equal concentrations. Additionally, no binding to or uptake of the fluorescent constructs into cells was observed with fluorescence microscopy regardless of cellular GPC3 expression level. In real-time radioligand binding experiments, very fast association and dissociation of the gallium-67 labeled peptides to GPC3 positive HepG2 cells was observed, suggesting either extremely fast binding kinetics or unspecific binding of the peptides. Conclusions: Taken together, these findings suggest that the peptide TJ12P2 lacks specific binding to GPC3 in vitro and might not serve as a basis for the development of radioligands targeting GPC3. Full article

Formaldehyde (FA) is commonly used for hatchery disinfection, where it reduces microbial growth, ensures successful egg hatch and enhances healthy production, but its specific effects on embryonic development remain unclear. MicroRNAs (miRNAs) regulate gene expression post-transcriptionally and may mediate FA-induced transcriptional responses. Here, we investigated the impact of FA treatment on miRNA profiles in chicken embryo liver. Small RNA-seq libraries were constructed and sequenced using the Illumina NextSeq platform. Reads were trimmed and quantified using miRDeep2 version 2.0.0.3. Differential expression analysis was performed with DESeq2 (p-adjusted < 0.05 and |log2FC| > 1). Target genes of differentially expressed miRNAs (DEMs) were predicted with miRDB, and GO/KEGG/Reactome enrichment was conducted. Out of 662 total mature miRNAs detected, differential expression analysis identified 30 DEMs (11 up-regulated, 19 down-regulated). The highest fold increase was determined for gga-miR-3533 (log2FC = 4.45), and the most significant decrease was determined for gga-miR-133b (log2FC = −3.38). Pathway analysis revealed miRNAs affecting signaling pathways along with modules related to post-translational protein modification, immune system, and oxidative stress pathways. Our study demonstrates that FA treatment can affect critical biological processes by altering miRNA-mediated regulation in the developing embryonic liver and point to the need for functional validation of miRNA-target interactions to help determine mechanisms for FA benefits. Long term, these data may help serve as reference to identify new treatments with optimized response profiles. Full article

Anastatus orientalis, a prominent egg parasitoid of Lycorma delicatula, demonstrates considerable potential for biological control. A. orientalis is dependent on host volatiles to identify and locate appropriate hosts for reproduction, with its olfactory system playing a vital role in volatile detection. There is little known about the chemosensory genes in A. orientalis. Therefore, here, we conducted a transcriptome analysis of the males and females from A. orientalis. Overall, 24 odorant binding proteins (OBPs), 4 chemosensory proteins (CSPs), 26 odorant receptors (ORs), 3 gustatory receptors (GRs), 3 ionotropic receptors (IRs), and 2 sensory neuron membrane proteins (SNMPs) were identified by transcriptome analysis. The values for fragments per kilobase per million (FPKM) indicated that the chemosensory protein gene families in A. orientalis exhibit different expression levels in male and female adults, with some genes showing significant differences and displaying sex-biased expression. Furthermore, RACE technology, phylogenetic analysis, and expression analysis were used to investigate the role that AoOBP8 plays in olfaction in A. orientalis. AoOBP8 was highly expressed in females and the heads of adults, indicating that the gene has a crucial role to play in the search for hosts and in oviposition in female adults, while the head is crucial in recognizing chemical information. These results contribute to a deeper understanding of the functions of chemosensory protein gene families in A. orientalis and offer a reference for developing biocontrol methods for forestry pests. Full article

Diabetic retinopathy (DR), a major complication of diabetes, is driven by chronic inflammation in which retinal microglial cells play a central role. The Hippo pathway kinases NDR1/2 regulate macrophage function, but their role in microglia and DR remain unknown. This study investigates the function of the NDR2 kinase in microglial cells under high-glucose (HG) conditions. Using CRISPR-Cas9, we partially knocked out the Ndr2/Stk38l gene in BV-2 mouse microglial cells and analyzed metabolic activity, phagocytosis, migration, and cytokine release. We confirmed NDR2 expression in microglia and observed increased levels under HG, suggesting a role in hyperglycemia-induced stress. Ndr2/Stk38l (hereafter referred to as Ndr2) downregulation impaired mitochondrial respiration and reduced metabolic flexibility, indicating defective stress adaptation. Functionally, microglia with a partial downregulation of Ndr2 displayed reduced phagocytic and migratory capacity—both dependent on cytoskeletal dynamics. Moreover, Ndr2 downregulation altered the secretory profile, elevating pro-inflammatory cytokines (IL-6, TNF, IL-17, IL-12p70) even under normal glucose levels. These findings identify NDR2 protein kinase as a key regulator of microglial metabolism and inflammatory behavior under diabetic conditions. By modulating immune and metabolic responses, NDR2 may contribute to the neuroinflammatory processes underlying DR. Targeting NDR2 function in microglia may offer novel therapeutic strategies to mitigate retinal inflammation and progression of DR. Full article

Vertical line source irrigation is a localized water-saving technique suitable for deep-rooted crops, but the geometric structure of the wetted bulb lacks a systematic analytical modeling method. This study established a simplified three-dimensional (3D) analytical model to predict the wetted volume under vertical line source irrigation conditions. First, the model determined boundary points based on an empirical wetting-front equation and fitted the wetting profile with ellipse–parabola functions to derive analytical expressions for area and volume. Then, using aeolian sandy soil as the research object, the model predicted that during 0–250 min of irrigation, the wetted pattern area increased from 80.0 cm² to 5050.6 cm², and the wetted volume increased from 251.3 cm³ to 208,014.4 cm³. At 250 min, the lower, middle, and upper volume components accounted for 67.3%, 24.2%, and 8.4%, respectively. Finally, the model was validated using loam soil, and the results showed good agreement between the calculated and measured values. The model requires only simple input and enables fast computation. It effectively characterizes the three-dimensional spatiotemporal variation of the wetted bulb and provides a theoretical reference for the design of pipe spacing and irrigation quota. Full article

Cultural ecosystem services (CES) represent fundamental expressions of human-environment interactions. A comprehensive assessment of CES supply and demand offers a robust scientific foundation for optimizing the transformation of ecosystem service values to improve human well-being. This study integrates multi-source datasets and employs Maximum Entropy (MaxEnt) modeling with the ArcGIS platform to analyze the spatial distribution of CES supply and demand in Hunan Province, a typical mountain tourism regions in China. Furthermore, geographical detector methods were used to identify and quantify the driving factors influencing these spatial patterns. The findings reveal that: (1) Both CES supply and demand demonstrate pronounced spatial heterogeneity. High-demand areas are predominantly concentrated around prominent scenic locations, forming a “multi-core, clustered” pattern, whereas high-supply areas are primarily located in urban centers, water systems, and mountainous regions, exhibiting a gradient decline along transportation corridors and river networks. (2) According to the CES supply-demand pattern, Hunan Province can be classified into demand, coordination, and enhancement zones. Coordination zones dominate (45–70%), followed by demand zones (20–30%), while enhancement zones account for the smallest proportion (5–20%). (3) Urbanization intensity and land use emerged as the primary drivers of CES supply-demand alignment, followed by vegetation cover, distance to water bodies, and population density. (4) The explanatory power of two-factor interactions across all eight CES categories surpasses that of any individual factor, highlighting the critical role of synergistic multi-factorial influences in shaping the spatial pattern of CES. This study provides a systematic analysis of the categories and driving factors underlying the spatial alignment between CES supply and demand in Hunan Province. The findings offer a scientific foundation for the preservation of ecological and cultural values and the optimization of spatial patterns in mountain tourist areas, while also serving as a valuable reference for the large-scale quantitative assessment of cultural ecosystem services. Full article

Per- and polyfluoroalkyl substances (PFASs) are persistent and bioaccumulative contaminants frequently detected in animal-derived foods, raising concerns for consumer health. In 2020, the European Food Safety Authority (EFSA) established a group tolerable weekly intake (TWI) of 4.4 ng/kg bw per week for four PFAS (PFOA, PFNA, PFOS, PFHxS) based on immunotoxicity, prompting the European Commission to set maximum levels in food. However, many other PFAS are present in the diet, and their cumulative risk is poorly characterized. This study applied the Relative Potency Factor (RPF) approach, using hepatic toxicity as the reference endpoint. The RPF approach addresses a key challenge in assessing human dietary exposure to PFAS by enabling cumulative risk assessment for complex mixtures found in food, moving beyond single-compound evaluations. Occurrence data from EFSA’s 2020 opinion were combined with European consumption data for fish, meat, eggs, and milk across four population groups (toddlers, adolescents, adults, elderly). Exposure estimates, expressed in PFOA equivalents, were compared with the group TWI. Results showed toddlers as the most vulnerable, with cumulative exposure approaching or exceeding TWI through fish, meat, and eggs, while milk contributed less. PFOS and PFOA were the main contributors across all food categories, with PFNA and PFDA also relevant, especially in younger populations. The findings highlight the added value of the RPF approach for cumulative PFAS risk assessment and emphasize the need for updated monitoring, refinement of potency factors for under-studied PFAS, and continued regulatory measures to protect high-risk consumers. Full article

CC motif chemokine ligand 20 (CCL20), a chemokine involved in immune cell migration through its receptor CCR6, has been implicated in kidney inflammation in crescentic glomerulonephritis and acute kidney injury. However, clinical information for other kidney diseases is scarce. We have analysed CCL20 levels in plasma and urine from patients with diabetic kidney disease (DKD, n = 98) and autosomal dominant polycystic kidney disease (ADPKD, n = 85) treated according to the guidelines and studied their association with baseline characteristics and long-term (median follow-up 4.9 and 7.1 years, respectively) clinical outcomes. Single-cell kidney transcriptomics were mined to identify CCL20-expressing cells. Plasma CCL20 was higher in DKD and ADPKD than in a reference group: median 12.8 (3.5–33.2), 6.0 (1.2–19.2), and 0.0 (0.0–9.0) pg/mL, respectively. Urinary CCL20 was quantifiable in 48% of patients with DKD but not in the reference group. Transcriptomics data support a local kidney source of CCL20. In DKD, plasma CCL20 was higher in early compared to advanced CKD. Urinary CCL20 was higher in patients with A2 albuminuria than in those with other albuminuria categories. In ADPKD, higher plasma and urinary CCL20 levels tended to be associated with lower eGFR, higher albuminuria, and larger kidneys. However, no significant association was found between CCL20 levels and progression to kidney failure or death. In conclusion, CCL20 is increased in biological fluids and locally produced in CKD. While this may point to a potential role in risk stratification, further studies are necessary. Full article

To obtain stable Chinese olive reference genes, eight genes (RPN2B, PIP1.4, NIFS1, RPS16, At5g12110, HSC-2, ABCG44, LOS1) exhibiting stable expression were identified as candidate reference genes from the transcriptome. The expression stability of these genes was evaluated across 33 Chinese olive fruit samples from different varieties and seven developmental stages. The most stable reference genes were determined through comparisons using ΔCq, geNorm, NormFinder, BestKeeper, and RefFinder. Analysis revealed that RPN2B and NIFS1 were consistently ranked among the most stable genes across the different algorithms and exhibited stable expression. Therefore, they are recommended as suitable reference genes for gene expression studies in Chinese olive fruits across different varieties and developmental stages. The four different methods of reference gene stability analysis were used to identify the most stable reference genes in different varieties and developmental stages of Chinese olive fruits, which can be used as a reference for the selection of reference genes in the subsequent gene expression studies of Chinese olive fruits. Full article

The ability to predict the vapor pressure and vapor-phase composition of hydrocarbon mixtures (such as jet fuel, sustainable aviation fuel or its un-refined precursors) and partially vaporized hydrocarbon mixtures is important to simulations of processes that involve vaporization such as distillations, flash points, combustion properties of partially vaporized fuels, etc. Raoult’s Law provides a simple algebraic formula relating liquid composition and temperature to vapor composition and pressure. However, Raoult’s Law is not accurate at low mole fractions, which is typical for complex mixtures such as fuels. A common approach to correcting Raoult’s Law is to apply a scale factor, a so-called activity coefficient. Numerous models exist for predicting activity coefficients. Here we benchmark against the UNIFAC model, which predicts activity coefficients based on mole fractions, group fractions, Van der Waals volume and surface area and temperature-dependent interaction terms between groups. While this approach is truly predictive, its accuracy at very low mole fractions has not been validated, and it is computationally intensive, particularly for simulations (especially optimizations) that require vapor composition or pressure within the inner-most loop. Here we present an alternative correction to Raoult’s law, where the vapor pressure of the i^th component is represented by a modified form of the Clausius–Clapeyron equation. The reference temperature ($T_{ref}$) is replaced by a simple algebraic function that converges to $T_{ref}$ as $x_i$ approaches 1 while smoothly increasing from this value as $x_i$ decreases. Simultaneously, the heat of vaporization ($\mathsf{\Delta }{H}_{vap,i}\left(T\right)$) term is replaced by another simple algebraic expression that converges to $\mathsf{\Delta }{H}_{vap,i}\left(T\right)$ as $x_i$ approaches 1 while smoothly decreasing as $x_i$ decreases. In this model, the temperature-dependent heat of vaporization is tuned at each temperature such that the Clausius–Clapeyron equation reproduces the correct vapor pressure of the neat material, while the parameterized algebraic corrections are tuned to vapor pressure data of mixtures involving n-pentane, toluene, and dodecane, where the mole fractions of n-pentane and toluene are maintained below 10%_mol. Validation of the resulting model is accomplished by comparing modeled vapor–liquid equilibrium systems with experimental measurements. This approach improves the accuracy and computational efficiency of volatility predictions, thereby supporting the development, certification, and adoption of sustainable aviation fuel. Full article