Search Results (36)

Shear wave velocity (V_S) measurements are widely used to characterize geomaterials, evaluate small-strain stiffness, and develop indirect approaches for estimating the liquefaction resistance of various soil types. In this study, the bender element method was employed to investigate the V_S characteristics of sand–gravel mixtures (SGMs), with the aim of clarifying the combined effect of key factors such as gravel content (G_C), relative density (D_r), packing state, and soil fabric. Laboratory tests were performed on reconstituted specimens composed of two sandy soils and pea gravel with G_C of 0, 10, 25, 40, 60, 80 and 100% and D_r of 20, 30, 45 and 60%. Specimens were prepared using wet tamping (WT) and air pluviation (AP) techniques. V_S measurements were conducted under effective confining stresses (${{\sigma }^{\prime }}_0$) of 50, 100, 150 and 200 kPa. The results show that the V_S of SGMs increases with increasing D_r and ${p^{\prime }}_0$, whereas the influence of G_C depends on the limiting and threshold sand contents. The effect of soil fabric was found to be marginal. Furthermore, the combined effects of G_C and D_r on V_S can be uniquely captured using the equivalent void ratio approach for SGMs with sand-dominated microstructures, while the skeleton void ratio approach is more appropriate for SGMs with gravel-dominated microstructures. Full article

Expansive clay slopes are vulnerable to progressive strength loss induced by repeated wetting and drying, a mechanism that drives shallow failure in active moisture zones. Reproducing this degradation experimentally is time-consuming and resource-intensive. This study evaluates whether Fully Softened Strength (FSS) can serve as a practical substitute for five wet–dry cycles in expansive clay slope stability assessment. Direct shear tests were conducted on wet–dry-cycled and reconstituted FSS specimens across fourteen experimental water contents. Strength parameters were incorporated into homogeneous and heterogeneous limit equilibrium slope models, considering degraded layer thicknesses of 1–5 m and suspended water table conditions. Equivalence was assessed using root mean square error (RMSE), prediction bias, and physical representativeness. Five wet–dry cycles produced a dominant cohesion reduction of 70.4% with minor changes in friction angle, reaching a quasi-stationary degraded state. FSS reproduced an equivalent system response through mechanical compensation between cohesion and friction—not through equality of strength parameters—under shallow failure conditions. The best statistical fit was obtained at w = 43.5% (RMSE = 0.314); however, w = 42.0%, coinciding with the liquid limit, provided a physically more robust interpretation with near-zero bias. Equivalence was found to be valid only for normal stresses ≤ 50 kPa, representative of shallow failure depths of 1–4 m. Full article

This study explores interpretation methods for determining the preconsolidation stress from one-dimensional consolidation test results. Twelve reconstituted clay specimens with targeted preconsolidation stresses of 60 and 120 kPa were prepared using commercial kaolinite and marine clays collected from coastal regions of South Korea. Five representative interpretation methods were applied, and the influence of maximum applied stress levels of 320, 640, and 1280 kPa was evaluated. The results indicate that the estimated preconsolidation stress varies considerably depending on both the interpretation method and the maximum applied stress, particularly for rounded compression curves with gradually changing virgin compression slopes. To address these limitations, a new interpretation approach is proposed. The method utilizes the rebound slope obtained from an unload–reload cycle and defines the virgin compression line through linear regression without identifying the recompression–virgin compression boundary. The proposed method demonstrated reduced sensitivity to the maximum applied stress and provided more reproducible estimates for rounded compression curves, although further validation using natural clays considering sample disturbance effects is required. Full article

High-water-content dredged slurry from port dredging requires geotechnical improvement via drainage and consolidation. The small-strain dynamic properties (shear stiffness, damping characteristics) of reconstituted and consolidated clays are critical to the dynamic response and serviceability of overlying infrastructure. This study uses resonant column tests to investigate how initial water content affects the small-strain dynamic behaviour of reconstituted Ningbo soft clay, focusing on the evolution of the dynamic shear modulus ($G$) and damping ratio ($\lambda$) under different initial water contents and confining pressures. The test results indicate that the initial water content exerts a pronounced effect on the maximum small-strain shear modulus ($G_{\mathrm{m}\mathrm{a}\mathrm{x}}$) and on the strain-dependent degradation pattern of $G$. $G_{\mathrm{m}\mathrm{a}\mathrm{x}}$ increases with decreasing water content, and confining pressure exerts a more pronounced enhancing effect on $G_{\mathrm{m}\mathrm{a}\mathrm{x}}$ under low water content conditions. For specimens with different initial water contents, the maximum shear modulus normalised by confining pressure $(G_{\mathrm{m}\mathrm{a}\mathrm{x}}/{({\sigma }_0^{\prime }/P_a)}^n)$ exhibits a consistent, material-specific functional relationship with void ratio ($e$) within the investigated ranges. By contrast, initial water content exerts limited effects on the normalised $G/G_{\mathrm{m}\mathrm{a}\mathrm{x}}-\gamma$ and $\lambda -\gamma$ curves in the tested small-strain range. On this basis, an empirical model for small-strain shear modulus incorporating initial water content effects is proposed to guide dynamic soil parameter selection for geotechnical design under the tested conditions. Full article

This study investigates the behaviour of dense silty sands with kaolinite clay under static drained/undrained conditions at low confining stress. Conventional laboratory tests assessed the mixtures’ physical properties, but standard void ratio methods proved inadequate for silty sands with kaolinite. Despite targeting 80% relative density, specimens exhibited loose sand behaviour in both drained and undrained tests. With increasing kaolinite content, conventionally reconstituted mixtures exhibit reduced peak stress ratios up to 10% fines, with little change beyond, while critical ratios generally rise at 25 kPa but remain unchanged or decrease slightly at 50 kPa. Analytical redefinition of minimum/maximum void ratios (based on sand–clay volumetric fractions) improved specimen reconstitution, yielding dense behaviour matching that of the host sand. The alternatively reconstituted mixtures display increasing drained peaks and minor changes in undrained peaks with increasing kaolinite content, with critical ratios increasing markedly at 25 kPa and only slightly at 50 kPa. However, this analytical void ratio determination method is limited to non-expansive, low-plasticity clays. Void ratios in silty sands with clay mineras are influenced by confining stress, drainage, saturation, clay content, and the sand skeleton structure. Unlike pure sands, these mixtures exhibit variable void ratios due to changes in the clay phase under different saturation levels. A new evaluation method is needed that accounts for clay composition, saturation-dependent consistency, and initial sand skeleton configuration to characterise these soils accurately. The findings highlight the limitations of conventional approaches and stress the need for advanced frameworks to model complex soil behaviour in geotechnical applications. Full article

Humanized mice generated by hematopoietic stem cell (HSC) transplantation are limited by the immune system developed being allogeneic to the tumor. We have innovated a platform to reconstitute an autologous human immune system (HIS) in immunodeficient NOG-EXL mice from mobilized peripheral blood (MPB)-CD34 cells, along with PDX generated from the same patient’s tumor tissue. Patients consented under an IRB-approved protocol for tumor biopsy and HSC apheresis at Emory University. HSC collection included mobilization with G-CSF and plerixafor, immunomagnetic bead isolation with CliniMACS, and cryopreservation of CD34⁺ cells. PDX were established from biopsies or surgical specimens by passaging into immunodeficient mice. Irradiated NOG-EXL mice were engrafted with HSCs by intravenous transplantation of CD34⁺ HSC. Engraftment of human T cells, B cells, and myeloid cells in peripheral blood was assessed by serial flow cytometry of blood samples, with final assessment of immune components in spleen and bone marrow at 30 weeks. Twenty-eight PDX models were generated from 43 patients with HNSCC; 1 patient underwent apheresis. HSC engraftment in blood was observed in 100% of NOG-EXL mice at 8 weeks post-transplant, with 5–20% hCD45⁺ cells present in the periphery. B-cell development was predominant at early time points and declined over time. Human T-cell and subset development of CD4⁺ and CD8⁺ T cells were observed in blood from 15 weeks post-transplant. Strong development of the myeloid lineage (CD33⁺) was observed starting at 8 weeks and persisted throughout the study. These data demonstrate that mobilization and apheresis of HNSCC patients is technically and clinically feasible and may allow the establishment of autologous HIS-PDX mice. Full article

Background: Thymoma-associated pure white cell aplasia (PWCA), characterized by agranulocytosis with absent myeloid precursors in the bone marrow in the setting of preserved erythropoiesis and megakaryopoiesis, is exceedingly rare, with only a few cases reported in the literature. We present a case of type-B2-thymoma-associated PWCA and immune reconstitution following marrow recovery. Case Presentation: A 75-year-old woman was incidentally found to have a concomitant mediastinal mass and peripheral leukopenia with absent granulocytes and monocytes. Bone marrow assessment was notable for a hypocellular marrow (<10%) with absent granulopoiesis and monopoiesis. Chest CT demonstrated a large lobulated anterior mediastinal mass, for which the patient underwent a video-assisted thoracoscopic thymectomy. Pathological evaluation of the mediastinal mass specimen revealed a type B2 thymoma. A tentative diagnosis of thymoma-associated PWCA was made, and the patient was started on cyclosporine/granulocyte-colony stimulating factor (G-CSF)/filgrastim therapy. Despite promising marrow recovery, she developed several comorbidities and had a leukemoid reaction, provoking concern for immune reconstitution following prolonged neutropenia and subsequent treatment. She passed away on post-operative day 15, and the results of a post-mortem bone marrow examination were consistent with granulocytic hyperplasia. Conclusions: This case of thymoma-associated PWCA heightens awareness regarding this entity, providing a note of caution regarding the possibility of immune reconstitution following treatment and marrow recovery. Full article

In order to investigate the influence of the coexistence of clay and silt on the compression characteristics of sand, one-dimensional compression consolidation tests were carried out on reconstituted saturated sand–silt–clay mixtures with a constant initial void ratio, and the effects of fines content (FC) and clay–silt ratio (CS) on the compression characteristics of mixed soils were studied. The mechanism of the experimental results was additionally explained from a microscopic perspective. The test results show that: the compressibility of mixed soil increased with the increase in FC; the compressibility change rule of mixed soils with different CS is consistent under the same FC; the influence of CS on the e–lgp (the void ratio (e) versus logarithm of the pressure (p)) curve of mixed soil is inconsistent when FC is different: when FC = 3%, the compressibility of mixed soil decreased with the increase in CS; when FC = 7% and 10%, the compressibility of mixed soil gradually increased with the increase in CS; when FC = 5%, the compressibility of mixed soil did not show an obvious changing law with the increase in CS, and the compressibility of the specimen with FC = 5%–CS = 1 (FC = 5%, CS = 1) was the largest; when CS was same, the difference between e–lgp curves of mixed soil with different FC increased with the increase in CS. The compression model of sand–silt–clay mixtures was established, which can consider the effects of FC and CS. The reliability and applicability of the proposed model were verified by combining the experimental results of this paper and the test data of sand–clay mixture and sand–silt mixture in other literature. Full article

The formation of layering during the sedimentation process of tailings makes it of great significance to investigate tailings and to analyze their susceptibility to flow liquefaction. In this study, homogeneous iron ore tailings (IOTs) specimens were reconstituted with pure coarser grains and pure finer grains sampled from a typical tailings storage facility. Additionally, an improved sample preparation method was developed to create heterogeneous IOTs samples containing a fine-grained interlayer with different thicknesses and dip angles using the above two materials. A series of standard drained and undrained triaxial compression tests were conducted to investigate the effects of the presence of a layered structure and its geometry on the stress–strain responses, and the properties of the IOTs under the critical state soil mechanics framework, which has been widely adopted in the analysis of liquefaction in mine tailings. The results showed that for the two homogeneous specimens, unique critical state lines (CSLs) can be identified, but they have different degrees of curvature in the e-ln p′ plane, causing a decrease in the susceptibility to liquefaction with increasing fines content. With increasing fine-grained interlayer thickness (FGLT) within 0–40 mm, the critical state friction angle (φ_cs) decreased steadily, while the CSLs in the e-ln p′ plane translated upward. This may be because the morphology of the microstructure within the fine-grained interlayer restricted the compression of the intergranular pores. With increasing fine-grained interlayer dip angle (FGLA) within the range 0–30°, φ_cs decreased until a discontinuity occurred at a dip angle of 15°, while the CSLs in the e-ln p′ plane rotated clockwise through a pivot point. Different FGLAs could change the contact area between the different layers and the axial distribution of the fine-grained interlayer and thus may further contribute to the rotation of the CSLs. Full article

This study shows the influence of the inclusion of abaca fiber (Musa Textilis) on the coefficients of consolidation, expansion, and compression for normally consolidated clayey silt organic soil specimens using reconstituted samples. For this purpose, abaca fiber was added according to the dry mass of the soil, in lengths (5, 10, and 15 mm) and concentrations (0.5, 1.0, and 1.5%) subjected to a curing process with sodium hydroxide (NaOH). The virgin and fiber-added soil samples were reconstituted as slurry, and one-dimensional consolidation tests were performed in accordance with ASTM D2435. The results showed a reduction in void ratio (compared to the soil without fiber) and an increase in the coefficient of consolidation (Cv) as a function of fiber concentration and length, with values corresponding to 1.5% and 15 mm increasing from 75.16 to 144.51 cm²/s. Although no significant values were obtained for the compression and expansion coefficients, it was assumed that the soil maintained its compressibility. The statistical analysis employed hierarchical linear models to assess the significance of the effects of incorporating fibers of varying lengths and percentages on the coefficients, comparing them with the control samples. Concurrently, mixed linear models were utilized to evaluate the influence of the methods for obtaining the Cv, revealing that Taylor’s method yielded more conservative values, whereas the Casagrande method produced higher values. Full article

The understanding of naturally occurring materials such as clay, sand, hard and soft rocks under a common theoretical framework has been a topic of persistent research interest. Over the past few decades, various sample reconstitution techniques have been developed in the literature to mimic in situ conditions, and to parse carefully the influence of various components in a cohesive-frictional geomaterial such that their behavior can be folded into the broad ambit of a continuum mechanics framework. The initial fabric of natural rock specimens is compared with reconstituted cemented sand samples using X-ray computed tomography (XRCT) scans. The efficacy of laboratory reconstitution techniques in replicating the initial microstructural features of natural rocks is evaluated here. Additionally, discrete element method (DEM) protocols which are often employed in generating cohesive granular ensembles are employed here and compared against the naturally occurring and artificially reconstituted fabric. A significant difference is observed in the grain boundaries of reconstituted and naturally occurring rocks. Additionally, the arrangement of particles, the orientation of grain contacts, and their coordination number are examined to assess the efficacy of laboratory-reconstituted specimens at micro-length scale. Full article

Kaolin clay, with its consistent properties, fine particle size, high surface area, and extensive historical use, stands out as a reliable choice for laboratory research. This study aims to assess the interface shear adhesion behaviour between compacted clay and a metallic surface. For this purpose, a new testing approach was developed. The proposed method is simple, requires neither advanced equipment nor specialised test procedures, and, thus, represents an improvement over existing practices in this field. The experimental program focuses on determining the interface shear adhesion strength between reconstituted kaolin clay and a metallic surface. The kaolin clay testing specimens were dynamically compacted at various energy levels and moisture contents. The results indicate that the optimum moisture content is 30%, which provides the highest density to the sample and divides the compaction curve into dry and wet sides. Furthermore, the results demonstrate that the interface shear adhesion strength increases with the clay’s dry density. Conversely, there is a significant decrease in strength as the moisture content specifically rises on the wet side of the compaction curve. The adhesion behaviour was also attributed to matric suction, where high suction enhanced interfacial adhesion, while low suction weakened bonding and diminished adhesion. Additionally, this study presents a unique three-dimensional contour graph illustrating the combined effects of dry density and moisture content on the interfacial adhesion. Full article

To investigate the effects of the properties of plastic fines on the pore water pressure generation characteristics of fine-grained soils during cyclic loadings, we used 29 sets of test data from the literature and prepared another 21 reconstituted specimens with different types of fines and fine contents (FCs) for cyclic triaxial testing. Two types of undisturbed soil specimens and three types of reconstituted soil specimens were also included for testing. The results indicated that under cyclic loading, the pore water pressure (PWP) ratios of clean sands increased slowly, stagnated, then finally accelerated until initial liquefaction, whereas those of the plastic soils containing fines with a plastic index (PI) value of >5 increased sharply in the initial stage. In addition, the cyclic stress ratio of specimens containing mudstone (PI = 12.4) and kaolinite (PI = 32.0) fines increased by 1.5–3.0 times more than non-plastic fines if the cyclic number chosen was 100. The range of the upper and lower limits of the PWP curves of the specimens with an FC of 30% were smaller that of the limits of the PWP curves of the specimens with an FC of 15%. The above results were further analyzed using a mathematical model. This paper systematically uses both the literature and laboratory test data to demonstrate that plastic fines and non-plastic fines have significantly different effects on water pressure generation under cyclic loading conditions, and a mathematical model also demonstrated the same trends. These findings are able to clarify previous unclear arguments. Thus, the model results developed in this study could also provide the field of engineering with a complete advanced calculation, requiring analysis only via software. Full article

Background: Opioids are considered the cornerstone of pain management: they show good efficacy as a first-line therapy for moderate to severe cancer pain. Since pharmacokinetic/pharmacodynamic information about the tissue-specific effect and toxicity of opioids is still scarce, their quantification in post-mortem autoptic specimens could give interesting insights. Methods: We describe an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry method for the simultaneous quantification of methadone, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone and fentanyl in several tissues: liver, brain, kidney, abdominal adipose tissue, lung and blood plasma. The presented method has been applied on 28 autoptic samples from different organs obtained from four deceased PLWH who used opioids for palliative care during terminal disease. Results: Sample preparation was based on tissue weighing, disruption, sonication with drug extraction medium and a protein precipitation protocol. The extracts were then dried, reconstituted and injected onto the LX50 QSight 220 (Perkin Elmer, Milan, Italy) system. Separation was obtained by a 7 min gradient run at 40 °C with a Kinetex Biphenyl 2.6 µm, 2.1 × 100 mm. Concerning the analyzed samples, higher opioids concentrations were observed in tissues than in plasma. Particularly, O-MOR and O-COD showed higher concentrations in kidney and liver than other tissues (>15–20 times greater) and blood plasma (>100 times greater). Conclusions: Results in terms of linearity, accuracy, precision, recovery and matrix effect fitted the recommendations of FDA and EMA guidelines, and the sensitivity was high enough to allow successful application on human autoptic specimens from an ethically approved clinical study, confirming its eligibility for post-mortem pharmacological/toxicological studies. Full article

Tectonic coal, an aggregate of coal particles formed by compacting pulverized coal, has been developed extensively in China. Currently, reconstituted coal specimens are widely adopted to investigate the mechanical properties of tectonic coal, but they have a low compaction degree compared to the tectonic coal in the field. Therefore, the current understanding of the mechanical properties of tectonic coal is not accurate. Herein, a new high–pressure–resistant mold was developed, and a heavy press was developed to prepare highly compacted reconstituted coal specimens. Based on the reconstituted coal specimens and the intact coal specimens obtained through coring, the mechanical properties of tectonic coal and intact coal were measured and compared systematically. The results show that the compaction degree of reconstituted coal specimen can be improved significantly by increasing the external force. For Sijiazhuang coal, the compaction degree of the reconstituted coal specimen almost reaches that of the tectonic coal in the field when the external force is increased to 550 KN. Moreover, the tectonic coal exhibits a low elastic modulus and low strength but high stress sensitivity. The elastic modulus and cohesion of tectonic coal are 22.08% and 43.47% of the corresponding values for intact coal. However, with the increase in the confining pressure from 5 to 20 MPa, the elastic modulus of tectonic coal increases by 1.14 times, while that of the intact coal increases just by 8.70%. In addition, tectonic coal and intact coal present different post-peak failure modes under the triaxial compression stress path. Typical shear failure occurs in the intact coal, while multiple shear failure occurs in the tectonic coal. Full article