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Keywords = quasi-static self-quenching

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13 pages, 5036 KB  
Review
Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics
by Simin Cao, Haoyang Li, Zenan Zhao, Sanjun Zhang, Jinquan Chen, Jianhua Xu, Jay R. Knutson and Ludwig Brand
Molecules 2021, 26(1), 211; https://doi.org/10.3390/molecules26010211 - 3 Jan 2021
Cited by 12 | Viewed by 6222
Abstract
In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, [...] Read more.
In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, reduced nicotinamide adenine dinucleotide (NADH), and nucleic acids. In the tryptophan dynamics field, especially for proteins, two types of solvation dynamics on different time scales have been well explored: ~1 ps for bulk water, and tens of picoseconds for “biological water”, a term that combines effects of water and macromolecule dynamics. In addition, some proteins also show quasi-static self-quenching (QSSQ) phenomena. Interestingly, in our more recent work, we also find that similar mixtures of quenching and solvation dynamics occur for the metabolic cofactor NADH. In this review, we add a brief overview of the emerging development of fluorescent RNA aptamers and their potential application to live cell imaging, while noting how ultrafast measurement may speed their optimization. Full article
(This article belongs to the Special Issue Probes for Detection, Sensing and Imaging)
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