Search Results (260)

Raceway ponds would allow the sustainable production of algal biomass because of their lower cost. However, for successful cultivation, the target organism needs to prevail despite unavoidable contamination by environmental strains. The development of efficient methods to control cyanobacterial proliferation is thus highly desirable. With the aim to identify new cyanobactericidal substances, a set of natural compounds was screened for the ability to inhibit the growth of a model cyanobacterial strain, Synechococcus elongatus PCC 6301. Three compounds, namely hydroquinone, juglone and plumbagin, were found to be active in the 10⁻⁶ to 10⁻⁴ M range. Activity was confirmed on a panel of 10 other cyanobacteria that showed different sensitivity, with concentrations causing 50% growth inhibition varying up to 2 orders of magnitude. Co-cultivation experiments showed that the growth of Microcystis aeruginosa PCC 7941 was almost completely suppressed at quinone concentrations at which that of Tolypothrix PCC 7601 was substantially unaffected. Juglone and plumbagin in the micromolar range also exerted toxic effects on eukaryotic microalgae, bacteria and yeast, whereas the growth of higher plants was affected only at higher concentrations. In the case of juglone, activity was lost with time after being dissolved, allowing water discharge/recycling. The results point at the aromatic 1.4-quinone/diol ring as a lead moiety for the development of chemicals to help maintaining monospecificity of microalgae cultures. Full article

Sediment buildup in storage tanks over extended operation periods may compromise their efficiency. To prevent pollutant deposition in storage tanks and enhance their hydraulic self-cleaning efficiency, this study addressed the unique structural configuration of lateral flow in storage tanks. Conducting numerical simulations to investigate the hydraulic characteristics within storage tanks, an integrated approach combining physical experiments and response surface methodology (RSM) was employed to optimize flow distribution. Key findings reveal that tangential and normal velocity differences lead to flow distribution nonuniformity, exacerbated by increased inflow Froude number (F_r) and reduced relative weir height (h_i). Based on the flow-splitting mechanism, an optimized “combined raised baffle” was proposed. Through single-factor experiments, Plackett–Burman (PB) screening, and RSM experiments, the optimal combination for maximal flow uniformity was determined as h₁ = 1.27, h₂ = 1.23, and h₃ = 1.24, achieving an 87.18% improvement in Q_y compared to the initial design. After optimization, the incoming flow pattern of the inlet channel of the storage pond was improved, and the difference between tangential and normal flow velocity in the flow field was significantly reduced. This research provides a novel approach and methodological paradigm for optimizing storage tanks and other hydraulic structures, demonstrating significant academic and engineering value. Full article

As metal resource extraction increases, heavy metal ion pollution in the saturated zone intensifies. Hence, research on the migration of heavy metal ions in aquifers and the efficacy of protective measures is essential to inform pollution prevention and control engineering. This study focuses on the slag pond and its surrounding area of a smelting plant. Utilizing field hydrological surveys and experiments, and data from previous studies, we employed FEFLOW7.0 simulation software to model the groundwater system of the boulder aquifer in this region. The model divides the domain based on natural topography: the eastern river serves as a constant-head boundary, while other areas are set as specified-flux boundaries. The impermeable layer at the bottom is treated as a no-flow boundary, with a maximum simulation period of 2500 days. The simulation examines the natural movement of zinc ions and how the construction of the wall impacts their migration, as well as the wall’s effectiveness in preventing seepage. Findings indicate that the movement of zinc ions is significantly influenced by the reaction coefficient. When the reaction coefficient exceeds 10⁻⁸ s⁻¹, zinc ions decrease rapidly in the area. After the construction of the cutoff wall, the maximum migration distance of zinc ions within 2500 days decreased from 220 m to 77 m, demonstrating its effectiveness in controlling zinc transport in groundwater. Full article

Building on the early investigation by Sidney W. Fox that dry-heated amino acids can spontaneously form microspheres, this research studies the self-organization of glycine and alanine with hydrogen in a liquid system. This study aimed to investigate the spontaneous formation of membraneless, microscale amino acid assemblies under simulated prebiotic hydrothermal conditions, such as hot mineral sources and ponds. Aqueous solutions of glycine and alanine were prepared in a hydrogen-rich mineral buffer and thermally incubated at 75 °C. Phase-contrast microscopy, transmission electron microscopy (TEM), and molecular modeling were employed to analyze the morphology and internal organization of the resulting structures. Microscopy revealed that zwitterionic glycine and alanine spontaneously self-organize into spherical microspheres (~12 µm), in which the charged –NH₃⁺ and –COO⁻ groups orient outward, while the hydrophobic methyl groups of alanine point inward, forming a stabilized internal core. The primary studies were performed with hot mineral water from Rupite, Bulgaria, at 73.4 °C. The resulting osmotic pressure difference Δπ ≈ 2490 Pa, derived from the van’t Hoff equalization. This suggests a chemically asymmetric system capable of sustaining directional water flux and passive molecular enrichment. The zwitterionic nature of glycine and alanine, which possesses both –NH₃⁺ and –COO⁻ groups, supports the formation of microspheres in our experiments. Under conditions with hot mineral water and hydrogen acting as a reducing agent in the primordial atmosphere, these amino acids self-organized into dense interfacial microspheres. These findings support the idea that thermally driven, zwitterion-mediated aggregation of simple amino acids, such as glycine and alanine, with added hydrogen, could generate membraneless, selectively organized microenvironments on the early Earth. Such microspheres may represent a plausible intermediate between dispersed organisms and microspheres. Full article

The dual benefit of wastewater and microalgal biomass is a major advantage of high-rate algal ponds, enabling the environmental valorization of these byproducts. This research explored the effect of treated wastewater on the agri-food species Hordeum vulgare (L.) and its associated weed, Emex spinosa (L.) Campd., along with the effects of algal biomass (primarily composed of Closterium, Chlorella, and Scenedesmus spp.) and Diplotaxis harra leaf powder. Initial pot trials applied microalgae and D. harra at 2, 4, and 6 g·kg⁻¹ soil, also confirming that the treated wastewater met reuse standards and did not affect plant growth. The combined treatment at 4 g·kg⁻¹ led to the highest H. vulgare increases in fresh weight (162.71%), root length (73.75%), and shoot length (72.87%), while reducing E. spinosa shoot and root lengths by 30.79% and 52.18%, and fresh weight by 68.24%. Subsequent field experiments using 1.26 t ha⁻¹ of 0.5-cm-applied D. harra and microalgae powders enhanced H. vulgare growth, while reducing the growth of E. spinosa. The reduction in E. spinosa growth was associated with increased electrolyte leakage and malondialdehyde content. These results support the integration of high-rate algal ponds into agriculture, promoting water reuse and reducing reliance on synthetic fertilizers and herbicides in barley production. Full article

Traditional intensive agricultural system impedes ecological functions, such as nutrient cycling and biodiversity conservation, resulting in excessive nitrogen discharge, CH₄ emission, and ecosystem service losses. To enhance critical ecosystem services and mitigate environmental externalities in paddy fields, we developed a multi-objective agricultural system (MIA system), which combines two eco-functional units: paddy wetlands and Beitang (irrigation water collection pond). Pilot study results demonstrated that the MIA system enhanced biodiversity and inhibited pest outbreak, with only a marginal reduction in rice production compared with the control. Additionally, the paddy wetland effectively removed nitrogen, with removal rates of total nitrogen and dissolved inorganic nitrogen ranging from 0.06 to 0.65 g N m⁻² d⁻¹ and from 0.02 to 0.22 g N m⁻² d⁻¹, respectively. Continuous water flow in the paddy wetland reduced the CH₄ emission by 84.4% compared with the static water conditions. Furthermore, a simulation experiment indicated that tide flow was more effective in mitigating CH₄ emission, with a 68.3% reduction compared with the drying–wetting cycle treatment. The emergy evaluation demonstrated that the MIA system outperformed the ordinary paddy field when considering both critical ecosystem services and environmental externalities. The MIA system exhibited higher emergy self-sufficiency ratio, emergy yield ratio, and emergy sustainable index, along with a lower environmental load ratio. Additionally, the system required minimal transformation, thus a modest investment. By presenting the case of the MIA system, we provide a theoretical foundation for comprehensive management and assessment of agricultural ecosystems, highlighting its significant potential for widespread application. Full article

Microalage are broadly recognized as promising agents for sustainable wastewater treatment and biomass generation. However, industrial effluents such as petroleum refinery wastewater (WW) present challenges due to toxic growth inhibiting substances. Three marine microalgae species: Pseudochloris wilhelmii, Nannochloropsis gaditana and Synechococcus sp. MK568070 were examined for cultivation potential in oil refinery WW. Their performance was evaluated in terms of growth dynamics, lipid productivity, and toxicity reduction, with a focus on their suitability for largescale industrial use. N. gaditana demonstrated the highest growth rate and lipid content (37% d.w.) as well as lipid productivity (29.45 mg/(Lday)) with the N-uptake rate of 0.698 mmol/(gday). The highest specific DIN uptake rate was observed inn P. wilhelmii (0.895 mmol/(gday) along with the highest volumetric productivity (93.9 mg/L/day) and WW toxicity removal (76.5%), while Synechococcus sp. MK568070 demonstrated lower performance metrics. A simple numerical model was applied to calculate continuous operation based on empirical results of batch experiments. Sustainability of the microalgae-based WW remediation under the conditions of optimized lipid biomass production was estimated, regarding 2019–2022–2025 cost dynamics. Parameters for optimum open raceway pond cultivation were calculated, and the biomass production accumulation was estimated, with the highest biomass production noted in P. wilhelmii (171.38 t/year). Comparison of treatment costs, production costs and revenue showed that the best candidate for WW remediation is N. gaditana. Full article

Aquaculture expansion to meet global protein demand has intensified concerns over nutrient pollution and greenhouse gas (GHG) emissions. While floating treatment wetlands (FTWs) are proven for water quality improvement, their potential to mitigate GHG emissions in marine aquaculture remains poorly understood. This study quantitatively evaluated the dual capacity of Sesuvium portulacastrum FTWs to (a) regulate dissolved inorganic nitrogen (DIN) and (b) reduce CO₂/N₂O emissions in grouper aquaculture systems. DIN speciation (NH₄⁺, NO₂⁻, NO₃⁻) and CO₂/N₂O fluxes of six controlled ponds (three FTW and three control) were monitored for 44 days. DIN in the FTW group was approximately 90 μmol/L lower than that in the control group, and the water in the plant group was more “oxidative” than that in the control group. The former groups were dominated by NO₃⁻, with lower dissolved inorganic carbon (DIC) and N₂O concentrations, whereas the latter were dominated by NH₄⁺ during the first 20 days of the experiment and by NO₂⁻ at the end of the experiment, with higher DIC and N₂O concentrations on average. Higher primary production may be the reason that the DIC concentration was lower in the plant group than in the control group, whereas efficient nitrification and uptake by plants reduced the availability of NH₄⁺ in the plant group, thereby reducing the production of N₂O. A comparison of the CO₂ and N₂O flux potentials in the plant group and control group revealed that, in the presence of FTWs, the CO₂ and N₂O emissions decreased by 14% and 36%, respectively. This showed that S. portulacastrum FTWs effectively couple DIN removal with GHG mitigation, offering a nature-based solution for sustainable aquaculture. Their low biomass requirement enhances practical scalability. Full article

With the rapid expansion of the aquaculture scale, the environmental pollution caused by the accumulation of fish pond sediment (FPS) has become increasingly prominent, making it urgent to establish sustainable resource utilization solutions. This study investigates the potential of using FPS as a soil amendment to improve saline–alkali soil (SAS) quality and enhance vegetable growth, while also quantifying ecological benefits through Gross Ecosystem Product (GEP) accounting. A pot experiment was conducted to evaluate the effects of different FPS mass percentages (0%, 20%, 40%, 80%, and 100%) on the growth of three vegetables (water spinach, lettuce, and chili) and soil quality. The results demonstrated that FPS addition at ≥40% significantly improves SAS quality, reducing the pH and salinity (p < 0.05), while enhancing organic matter, nutrient availability, and microbial activity. Among the treatments, 80% FPS maximized vegetable yields, with water spinach achieving the highest edible biomass (37.32 g). Compared to the control, nutritional quality under ≥80% FPS treatment showed substantial increases: vitamin C (133.33–307.03%), soluble sugars (49.97–73.53%), and protein (26.14–48.08%). An economic analysis revealed that 80% FPS with water spinach cultivation generated peak ecological benefits (274,951 CNY·ha⁻¹; 185% above control). These findings provide a scientific basis and effective model for the resource utilization of FPS and the improvement of saline–alkali soil, offering significant implications for the sustainable development of agriculture and environmental protection. Full article

(1) Although lotus ponds exhibit ecological benefits in wetland restoration, their efficacy in water purification and eutrophication mitigation remains unclear. (2) This study utilized Jinluo lotus pond as the experimental group and the adjacent river as the control. Five sampling points were established in each area, with water samples collected in June 2022, April 2025, and May 2025. (3) The pH, BOD, COD, TN, and NH₃-N concentrations in Jinluo lotus pond water are higher than those in rivers, while the TP, NO₃-N, Chl-a, and algal cell density in rivers are higher. However, there was no significant difference in the nine parameters (p > 0.05) in June 2022. The pH, DO, algal cell density, and algal biomass of the Jinluo lotus pond were significantly higher (p < 0.05 for DO); the concentrations of BOD, COD, TN, TP, NH₃-N, NO₃-N, PI, and Chl-a in rivers are higher, with significant differences in Chl-a (p < 0.05) in April 2025. The BOD, COD, TP, NO₃-N, and PI of the Jinluo lotus pond were relatively high (p < 0.05 for PI); the pH, TN, NH₃-N, DO, Chl-a, algal cell density, and algal biomass of rivers are higher, with significant differences in Chl-a (p < 0.05) in May 2025. The results showed that there was no significant difference in the four diversity indicators in June 2022, April 2025, and May 2025. There was no significant difference in the algal diversity indices, including species richness (S), Shannon–Wiener diversity index (H), Simpson diversity index (P), and Pielou evenness index (E) between Jinluo lotus pond and rivers. (4) Conclusions and Recommendations: The Jinluo lotus pond and adjacent rivers suffer from severe nutrient overload, especially with BOD, COD, and TN all being classified as Class 5 water. Expanding natural and constructed reed communities is recommended to enhance nutrient removal. However, given the limited purification capacity of lotus ponds, maintaining or increasing their area may not be justified. Full article

Forest thinning treatments are expanding in scope and scale to counter increasing wildfire risk. Such treatments are being applied in aquatic-adjacent forests that provide a critical habitat for sensitive amphibians, yet little is known about the impact of these treatments. We used a 5-year (2017–2021) before–after–control–impact experiment to investigate the effects of hand-thinning on pond-breeding amphibian activity around an ephemeral lake in the southern Cascade Range of California. We found that hand-thinning had no detectable negative effects on long-toed salamanders (Ambystoma macrodactylum) and western toads (Anaxyrus boreas) and significantly increased adult Sierran chorus frog (Pseudacris sierra) activity (χ² = 4.70, df = 1, p = 0.030) in upland habitats. These results are consistent with pre-treatment habitat associations—chorus frog activity was higher when tree density was lower, and the treatment reduced tree density; adult long-toed salamanders and western toads were positively associated with canopy closure, which was not significantly reduced by the treatment. In addition, late-season surface activity of adult long-toed salamanders and chorus frogs was strongly associated with fall rain events when they tend to cluster very near the lake edge. Hand-thinning in aquatic-adjacent habitat may have minimal negative impacts, and even some positive impacts, on pond-breeding amphibians in coniferous forests, especially if treatments are not implemented during periods of high amphibian activity and do not substantially alter canopy closure. Full article

Fiber optic distributed acoustic sensing (DAS) technology can monitor vibrational strain of vast areas with fine spatial resolution at high sampling rates. The fiber optic cable portion of DAS may directly monitor, measure, and map potentially unsafe areas such as thin ice sheets. Once the fiber optic cable is emplaced, DAS can provide “rapid-response” information along the cable’s length through remote sampling. A field campaign was performed to test the sensitivity of DAS to spatial variations within thin ice sheets. A pilot field study was conducted in the northeastern United States in which fiber-optic cable was deployed on the surface of a freshwater pond. Phase velocity transformations were used to analyze the DAS response to strike testing on the thin ice sheet. The study results indicated that the ice sheet was about 5 cm thick generally, tapering to about 3.5 cm within 2 m of the pond’s edge and then disappearing at the margins. After validation of the pilot study’s methodology, a follow-up experiment using DAS to collect on a rapidly deployed, surface-laid cable atop a larger freshwater pond was conducted. Using phase velocity transformations, the ice thickness along the fiber optic cable was estimated to be between 25.5 and 28 cm and confirmed via ice auger measurements along the fiber optic cable. This field campaign demonstrates the feasibility of employing DAS systems to remotely assess spatially variable properties on thin freshwater ice sheets. Full article

At present, there is a lack of effective and usable machinery in the harvesting of aquatic vegetables. The harvesting of most aquatic vegetables such as Brasenia schreberi relies entirely on manual labor, resulting in a high labor demand and labor shortages, which restricts the industrial development of aquatic vegetables. To address this problem, an intelligent harvesting system for the aquatic vegetable Brasenia schreberi was developed in response to the challenging working conditions associated with harvesting it. The system is composed of a catamaran mobile platform, a picking device, and a harvesting manipulator control system. The mobile platform, driven by two paddle wheels, is equipped with a protective device to prevent vegetable stem entanglement, making it suitable for shallow pond aquatic vegetable environments. The self-designed picking device rapidly harvests vegetables through lateral clamping and cutting. The harvesting manipulator control system incorporates harvesting posture perception based on the YOLO-GS recognition algorithm and combines it with an improved RRT algorithm for robotic arm path planning. The experimental results indicate that the intelligent harvesting system is suitable for aquatic vegetable harvesting and the improved RRT algorithm surpasses the traditional one in terms of the planning time and path length. The vision-based positioning error was 4.80 mm, meeting harvesting accuracy requirements. In actual harvest experiments, the system showed an average success rate of 90.0%, with an average picking time of 5.229 s per leaf, thus proving its feasibility and effectiveness. Full article

Thus far, various aquaculture modes have been developed to facilitate the rapid growth of the aquaculture industry and thus meet the heavy demand for aquatic products for human consumption. However, the effects of different culture modes on fish muscular nutritional content and volatile flavor are rarely reported. In the present study, we evaluated the differences in muscular nutrition content and dietary flavor between Chinese longsnout catfish (Leiocassis longirostris) groups cultured in two different modes, i.e., flow-through water tanks (CWWL) and traditional ponds (CWWC). Our statistical results showed that a significantly higher crude protein content and lower crude fat levels were observed in the CWWL group than in the CWWC group (p < 0.05). Similarly, the contents of total aromatic amino acids (Total ∑TAA) and total dicarboxylic amino acids (Total ∑DAA) were also significantly higher in the CWWL group. Among the fatty acids, long-chain polyunsaturated fatty acids (LC-PUFAs), including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (ARA), were recorded at 1.44%, 2.5%, and 9.09%, respectively, in the CWWL group, which were dramatically higher than in the CWWC group. Conversely, the contents of volatile compounds, including 2-tridecanone, dimethyl trisulfide, and isophorone, in the CWWC group were also significant higher, which, however, may contribute to an unpleasant sensory experience. Conversely, other compounds like 2-methylbutanal and 2,3-butanedione were prevalent in the CWWL group, which can induce rich nutty and buttery flavors and thus enhance the freshness of flavor profiles. In conclusion, Chinese longsnout catfish cultured in flow-through tanks show higher nutritional value and better sensory flavor in comparison with those raised in ponds. These findings not only provide novel insights into the potential effects of aquaculture modes on muscular nutrition content and dietary flavor for Chinese longsnout catfish but also lay a solid foundation for optimizing practical culture modes to improve the global aquaculture industry. Full article

This study investigated the effects of culture density on the phytoplankton communities in an integrated culture model of the blood clam Tegillarca granosa and Pacific white shrimp Litopenaeus vannamei. Two treatments were set up: L (L. vannamei 18 ind/m², T. granosa 33 ind/m²) and H (L. vannamei 36 ind/m², T. granosa 66 ind/m²). The management methods were the same during the experimental period, and the feed intake was determined according to L. vannamei. The results showed that there were more than 38 species of phytoplankton in the ponds, belonging to six phyla and 28 genera; the diversity index of the phytoplankton was higher in the early stage of the experiment and lower in the later stage; the average biomass of phytoplankton was 21.74 × 10⁴ ind/L; and the culture density had no significant effect on water quality. This study showed that a reasonable increase in culture density would not cause significant adverse effects on the environment. It is possible to increase the stocking density of shrimp with the mollusk without causing inadequate conditions for the phytoplankton community and water quality. Full article