Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (2)

Search Parameters:
Keywords = nanolaccase

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
12 pages, 2971 KiB  
Article
Highly Sensitive Amperometric Sensor Based on Laccase-Mimicking Metal-Based Hybrid Nanozymes for Adrenaline Analysis in Pharmaceuticals
by Olha Demkiv, Nataliya Stasyuk, Galina Gayda and Mykhailo Gonchar
Catalysts 2021, 11(12), 1510; https://doi.org/10.3390/catal11121510 - 11 Dec 2021
Cited by 10 | Viewed by 3059
Abstract
Nanozymes are nanomaterials which exhibit artificial enzymatic activities and are considered as alternatives to natural enzymes. They are characterized by good catalytic activity and high stability, as well as ease and low cost of preparation. In this study, the mimetics of laccase or [...] Read more.
Nanozymes are nanomaterials which exhibit artificial enzymatic activities and are considered as alternatives to natural enzymes. They are characterized by good catalytic activity and high stability, as well as ease and low cost of preparation. In this study, the mimetics of laccase or “nanolaccases” (NLacs) were synthesized by a simple method of chemical reduction of transition metal salts. The NLacs were tested for their catalytic activity in solution and on the electrode surface. The most effective NLacs, namely nAuCePt and nPtFe, were found to possess excellent laccase-like activities capable of oxidizing the endocrine hormone adrenaline (AD). These NLacs were characterized in detail and used for the development of amperometric sensors for AD determination. The amperometric sensors containing the best NLacs, as well as a natural fungal laccase, were constructed. The most effective nAuCePt-containing sensor had good specificity in relation to AD and improved analytical characteristics. It possessed a 384-fold higher sensitivity than adrenaline (230,137 A·M−1·m−2), a 64-fold lower limit of detection (0.025 µM), and a broader linear range (0.085–45 µM) in comparison with the sensor based on natural laccase. The constructed nAuCePt-containing sensor was successfully used for AD analysis in pharmaceutical formulation. Full article
(This article belongs to the Special Issue Enzymes in Materials Science)
Show Figures

Graphical abstract

42 pages, 4930 KiB  
Review
Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review
by Nataliya Stasyuk, Oleh Smutok, Olha Demkiv, Tetiana Prokopiv, Galina Gayda, Marina Nisnevitch and Mykhailo Gonchar
Sensors 2020, 20(16), 4509; https://doi.org/10.3390/s20164509 - 12 Aug 2020
Cited by 93 | Viewed by 9617
Abstract
The current review is devoted to nanozymes, i.e., nanostructured artificial enzymes which mimic the catalytic properties of natural enzymes. Use of the term “nanozyme” in the literature as indicating an enzyme is not always justified. For example, it is used inappropriately for nanomaterials [...] Read more.
The current review is devoted to nanozymes, i.e., nanostructured artificial enzymes which mimic the catalytic properties of natural enzymes. Use of the term “nanozyme” in the literature as indicating an enzyme is not always justified. For example, it is used inappropriately for nanomaterials bound with electrodes that possess catalytic activity only when applying an electric potential. If the enzyme-like activity of such a material is not proven in solution (without applying the potential), such a catalyst should be named an “electronanocatalyst”, not a nanozyme. This paper presents a review of the classification of the nanozymes, their advantages vs. natural enzymes, and potential practical applications. Special attention is paid to nanozyme synthesis methods (hydrothermal and solvothermal, chemical reduction, sol-gel method, co-precipitation, polymerization/polycondensation, electrochemical deposition). The catalytic performance of nanozymes is characterized, a critical point of view on catalytic parameters of nanozymes described in scientific papers is presented and typical mistakes are analyzed. The central part of the review relates to characterization of nanozymes which mimic natural enzymes with analytical importance (“nanoperoxidase”, “nanooxidases”, “nanolaccase”) and their use in the construction of electro-chemical (bio)sensors (“nanosensors”). Full article
(This article belongs to the Special Issue Biosensors – Recent Advances and Future Challenges)
Show Figures

Figure 1

Back to TopTop