Search Results (21)

The tectonic evolution of Hainan Island during the Late Permian–Early Triassic period is still unclear. This study identified two types of basalts on the island and presented detailed geochronology, whole-rock geochemistry, and Hf isotope data of the Late Permian–Early Triassic basalts. U-Pb dating results indicated that baddeleyites and zircons of one sample from Group 1 basalts had formation ages of 256 ± 3 Ma and 255 ± 3 Ma, respectively, and two samples from Group 2 gave formation ages of 241 ± 2 Ma and 240 ± 3 Ma, respectively. Both groups are characterized by negative anomalies of Nb, Ta, and Ti, and enrichment in Ba, Th, U, and K. Group 1 belongs to sub-alkaline basalt and exhibited SiO₂ contents ranging from 50.50% to 51.05%, with ΣREE concentration of 136–148 ppm. Hf isotope analysis showed that the ε_Hf(t) values of baddeleyites and zircons were −10.56 to −4.70 and −14.94 to −6.95, respectively. Group 2 belongs to alkaline basalt and had a higher SiO₂ content of 52.48%–55.49% and ΣREE concentration of 168–298 ppm. They showed more depleted Hf isotopic composition with ε_Hf(t) values ranging from −2.82 to +4.74. These data indicate that the source area of Group 1 was an enriched mantle, likely derived from partial melting of spinel lherzolite mantle, and was modified by subduction-derived fluids. Group 2 was derived from depleted mantle, most likely originating from partial melting of garnet + spinel lherzolite mantle. They were contaminated by crustal materials and metasomatized by subduction-derived fluids with a certain degree of fractional crystallization. Comprehensive analysis suggests that Group 1 samples likely formed in an island arc tectonic setting, while Group 2 formed in a continental intraplate extensional (or initial rift) tectonic setting. Their formation was mainly controlled by the Paleo-Tethys tectonic domain. Group 1 basalts implied that subduction of the Paleo-Tethys oceanic crust lasted at least in the late Permian (ca. 255 Ma). Group 2 basalts revealed that the intra-plate extensional (or initial rift) stage occurred in the middle Triassic (ca. 240 Ma). Full article

Bovine herpesvirus type 1 (BoHV-1) establishes lifelong latency in trigeminal ganglionic (TG) neurons following intranasal and ocular infection in cattle. Periodically, the latent virus reactivates in the TG due to stress and is transported anterogradely to nerve endings in the nasal epithelium, where the virus replicates and sheds. Consequently, BoHV-1 is transmitted to susceptible animals and maintained in the cattle population. Modified live BoHV-1 vaccine strains (BoHV-1 MLV) also have a similar latency reactivation. Therefore, they circulate and are maintained in cattle herds. Additionally, they can regain virulence and cause vaccine outbreaks because they mutate and recombine with other circulating field wild-type (wt) strains. Recently, we constructed a BoHV-1 quadruple mutant virus (BoHV-1qmv) that lacks immune evasive properties due to UL49.5 and glycoprotein G (gG) deletions. In addition, it also lacks the gE cytoplasmic tail (gE CT) and Us9 gene sequences designed to make it safe, increase its vaccine efficacy against BoHV-1, and restrict its anterograde neuronal transport noted above. Further, we engineered the BoHV-1qmv-vector to serve as a subunit vaccine against the Rift Valley fever virus (BoHV-1qmv Sub-RVFV) (doi: 10.3390/v15112183). In this study, we determined the latency reactivation and nasal virus shedding properties of BoHV-1qmv (vector) and BoHV-1qmv-vectored subunit RVFV (BoHV-1qmv sub-RVFV) vaccine virus in calves in comparison to the BoHV-1 wild-type (wt) following intranasal inoculation. The real-time PCR results showed that BoHV-1 wt- but not the BoHV-1qmv vector- and BoHV-1qmv Sub-RVFV-inoculated calves shed virus in the nose following dexamethasone-induced latency reactivation; however, like the BoHV-1 wt, both the BoHV-1qmv vector and BoHV-1qmv Sub-RVFV viruses established latency, were reactivated, and replicated in the TG neurons. These results are consistent with the anterograde neurotransport function of the gE CT and Us9 sequences, which are deleted in the BoHV-1qmv and BoHV-1qmv Sub-RVFV. Full article

The use of effective vaccines is among the most important strategies for the prevention and progressive control of transboundary infectious animal diseases. However, the use of vaccine is often impeded by the cost, a lack of cold chains and other factors. In resource-limited countries in Africa, one approach to improve coverage and reduce cost is to vaccinate against multiple diseases using combined vaccines. Therefore, the objective of this study was to evaluate a combined vaccine for the prevention and control of Lumpy Skin Disease (LSD), Contagious Bovine Pleuropneumonia (CBPP) and Rift Valley fever (RVF). The LSD and CBPP were formulated as a combined vaccine, and the RVF was formulated separately as live attenuated vaccines. These consisted of a Mycoplasma MmmSC T1/44 strain that was propagated in Hayflick-modified medium, RVF virus vaccine, C13T strain prepared in African green monkey cells (Vero), and the LSDV Neethling vaccine strain prepared in primary testis cells. The vaccines were tested for safety via the subcutaneous route in both young calves and pregnant heifers with no side effect, abortion or teratogenicity. The vaccination of calves induced seroconversions for all three vaccines starting from day 7 post-vaccination (PV), with rates of 50% for LSD, 70% for CBPP and 100% for RVF, or rates similar to those obtained with monovalent vaccines. The challenge of cattle vaccinated with the LSD/CBPP and the RVF vaccine afforded full protection against virulent strains of LSDV and RVFV. A satisfactory level of protection against a CBPP challenge was observed, with 50% of protection at 6 months and 81% at 13 months PV. A mass vaccination trial was performed in four regions of Burkina Faso that confirmed safety and specific antibody responses induced by the vaccines. The multivalent LSD/CBPP+RVF vaccine provides a novel and beneficial approach to the control of the three diseases through one intervention and, therefore, reduces the cost and improves vaccination coverage. Full article

We have previously shown in model studies that rapid quenches of systems of monomers interacting to form polymer chains can fix nonequilibrium chemistries with some lifelike properties. We suggested that such quenching processes might have occurred at very high rates on early Earth, giving an efficient mechanism for natural sorting through enormous numbers of nonequilibrium chemistries from which the most lifelike ones could be naturally selected. However, the model used for these studies did not take account of activation barriers to polymer scission (peptide bond hydrolysis in the case of proteins). Such barriers are known to exist and are expected to enhance the quenching effect. Here, we introduce a modified model which takes activation barriers into account and we compare the results to data from experiments on quenched systems of amino acids. We find that the model results turn out to be sensitive to the width of the distribution of barrier heights but quite insensitive to its average value. The results of the new model are in significantly better agreement with the experiments than those found using our previous model. The new parametrization of the model only requires one new parameter and the parametrization is more physical than the previous one, providing a chemical interpretation of the parameter p in our previous models. Within the model, a characteristic temperature $T_c$ emerges such that if the temperature of the hot stage is above $T_c$ and the temperature of the cold stage is below it, then the ‘freezing out’, in a quench, of a disequilibrium ensemble of long polymers is expected. We discuss the possible relevance of this to models of the origin of life in emissions from deep ocean rifts. Full article

We applied teleseismic tomography to investigate the 3D P-wave velocity (Vp) structure of the crust and upper mantle at depths of 50–400 km beneath the Yarlung–Tsangpo suture (YTS), by using 6164 P-wave relative travel-time residuals collected from 495 teleseismic events recorded at 20 three-component broadband seismograms. A modified multi-channel cross-correlation method was adopted to automatically calculate the relative arrival-time residuals of all teleseismic events, which significantly improved the efficiency and precision of the arrival-time data collection. Our results show that alternating low- and high-Vp anomalies are visible beneath the Himalayan and Lhasa blocks across the YTS, indicating that strong lateral heterogeneities exist beneath the study region. A significant high-Vp zone is visible beneath the southern edge of the Lhasa block at 50–100 km depths close to the YTS, which might indicate the rigid Tibetan lithosphere basement. There exists a prominent low-Vp zone beneath the Himalayan block to the south of the YTS extending to ~150 km depth, which might be associated with the fragmentation of the underthrusting Indian continental lithosphere (ICL) and induce localized upwelling of asthenospheric materials from the upper mantle. In addition, significant low-Vp anomalies were observed beneath the Yadong–Gulu rift and the Cona–Sangri rift extending to ~300 km depth, indicating that the tearing of the subducted ICL might provide pathways for the localized asthenospheric materials upwelling, which contributes to the widespread distribution of north–south trending rifts and geothermal activities in southern Tibet. Full article

In this paper, certain areas of the Kingdom of Saudi Arabia (KSA) are assessed in order to map potential geothermal energy zones. To evaluate high-resolution aerial magnetic data, spectral depth analysis using a modified centroid approach was used. The calculated geothermal parameters were gridded in order to delineate the regions characterised by a shallow Curie point depth (CPD) and a high geothermal gradient (GG) as well as a high heat flow (HF). The CPD, GG and HF calculated from the analysed data varied in the ranges of 6.0–15.0 km, 40.0–100.0 °C/km and 90.0–270.0 mW/m², respectively. The obtained results show the concurrence of the positions of shallow CPD (<8.0 km), high GG (>83.5 °C/km) and high HF (>211.0 mW/m²). The geothermal systems that are oriented in the E–W direction are related to the Red Sea tectonics, the tectonic opening of the Red Sea/Gulf of the Suez Rift, hot subterranean anomalies and high enthalpy from radioactive granites. Likewise, the geologic structures (fractures and faults) related to the Red Sea tectonics serve as channels for the movement of hydrothermal fluids and the deposition of associated minerals. All in all, another geophysical study involving deep boreholes, and seismic, magnetotelluric, electromagnetic and geochemical data should be conducted to evaluate and estimate precisely the economic reserves of geothermal resources. Full article

High-resolution thermal infrared (TIR) remote sensing images can more accurately retrieve land surface temperature and describe the spatial pattern of urban thermal environment. The Thermal Infrared Spectrometer (TIS), which has high spatial resolution among spaceborne thermal infrared sensors at present, and global data acquisition capability, is one of the sensors equipped in the SDGSAT-1. It is an important complement to the existing international mainstream satellites. In order to produce standard data products, rapidly and accurately, the automatic registration and geometric correction method needs to be developed. Unlike visible–visible image registration, thermal infrared images are blurred in edge details and have obvious non-linear radiometric differences from visible images, which make it challenging for the TIR-visible image registration task. To address these problems, homomorphic filtering is employed to enhance TIR image details and the modified RIFT algorithm is proposed to achieve TIR-visible image registration. Different from using MIM for feature description in RIFT, the proposed modified RIFT uses the novel binary pattern string to descriptor construction. With sufficient and uniformly distributed ground control points, the two-step orthorectification framework, from SDGSAT-1 TIS L1A image to L4 orthoimage, are proposed in this study. The first experiment, with six TIR-visible image pairs, captured in different landforms, is performed to verify the registration performance, and the result indicates that the homomorphic filtering and modified RIFT greatly increase the number of corresponding points. The second experiment, with one scene of an SDGSAT-1 TIS image, is executed to test the proposed orthorectification framework. Subsequently, 52 GCPs are selected manually to evaluate the orthorectification accuracy. The result indicates that the proposed orthorectification framework is helpful to improve the geometric accuracy and guarantee for the subsequent thermal infrared applications. Full article

Rainfall and temperature trends detection is vital for water resources management and decision support systems in agro-hydrology. This study assessed the historical (1983–2005) and future (2026–2100) rainfall, maximum temperature (T_max), and minimum temperature (T_min) trends of the Ziway Lake Basin (Ethiopia). The daily observed rainfall and temperature data at eleven stations were obtained from the National Meteorological Agency (NMA) of Ethiopia, while simulated historical and future climate data were obtained from the Coupled Model Intercomparison Project 5 (CMIP5) datasets under Representative Concentration Pathways (RCP) of 4.5 and 8.5. The CMIP5 datasets were statistically downscaled by using the climate model data for hydrologic modeling (CMhyd) tool and bias corrected using the distribution mapping method available in the CMhyd tool. The performance of simulated rainfall, T_max, and T_min of the CMIP5 models were statistically evaluated using observation datasets at eleven stations. The results showed that the selected CMIP5 models can reasonably simulate the monthly rainfall, T_max, and T_min at the majority of the stations. Modified Mann–Kendall trend test were applied to estimate the trends of annual rainfall, T_max, and T_min in the historical and future periods. We found that rainfall experienced no clear trends, while T_max, and T_min showed consistently significant increasing trends under both RCP 4.5 and 8.5 scenarios. However, the warming is expected to be greater under RCP 8.5 than RCP 4.5 by the end of the 21st century, resulting in an increasing trend of T_max and T_min at all stations. The greatest warming occurred in the central part of the basin, with statistically significant increases largely seen by the end of the 21st century, which is expected to exacerbate the evapotranspiration demand of the area that could negatively affect the freshwater availability within the basin. This study increases our understanding of historic trends and projected future change effects on rainfall- and evapotranspiration-related climate variables, which can be used to inform adaptive water resource management strategies. Full article

Isotopic and geochemical data delineate passive margin, rift and active margin cycles in northwestern South America since ~623 Ma, spanning from the Iapetus Wilson Cycle. Ultramafic and mafic rocks record rifting associated with the formation of the Iapetus Ocean during 623–531 Ma, while the initiation of subduction of the Iapetus and Rheic oceans is recorded by continental arc plutons that formed during 499–414 Ma, with alternating compressive and extensional stages. Muscovite ⁴⁰Ar/³⁹Ar dates suggest there may have been a phase of Carboniferous metamorphism, although this remains tentative. A Passive margin was modified by active margin magmatism that started at ~294 Ma and culminated with collisional tectonics that signaled the final stages of the amalgamation of western Pangaea. Early Pangaea fragmentation included back-arc rifting during 245–216 Ma, leading to a Pacific active margin that spanned from 213–115 Ma. Trench retreat accelerated during 144–115 Ma, forming a highly attenuated continental margin prior to the collision of the Caribbean Large Igneous Province at ~75 Ma. Full article

Many biological and biotechnological processes are controlled by protein–protein and protein–solvent interactions. In order to understand, predict, and optimize such processes, it is important to understand how solvents affect protein structure during protein–solvent interactions. In this study, all-atom molecular dynamics are used to investigate the structural dynamics and energetic properties of a C-terminal domain of the Rift Valley Fever Virus L protein solvated in glycerol and aqueous glycerol solutions in different concentrations by molecular weight. The Generalized Amber Force Field is modified by including restrained electrostatic potential atomic charges for the glycerol molecules. The peptide is considered in detail by monitoring properties like the root-mean-squared deviation, root-mean-squared fluctuation, radius of gyration, hydrodynamic radius, end-to-end distance, solvent-accessible surface area, intra-potential energy, and solvent–peptide interaction energies for hundreds of nanoseconds. Secondary structure analysis is also performed to examine the extent of conformational drift for the individual helices and sheets. We predict that the peptide helices and sheets are maintained only when the modeling strategy considers the solvent with lower glycerol concentration. We also find that the solvent-peptide becomes more cohesive with decreasing glycerol concentrations. The density and radial distribution function of glycerol solvent calculated when modeled with the modified atomic charges show a very good agreement with experimental results and other simulations at $298.15$$\mathrm{K}$. Full article

Non-transform discontinuity (NTD) is one category of tectonic units along slow- and ultraslow-spreading ridges. Some NTD-related hydrothermal fields that may reflect different driving mechanisms have been documented along slow-spreading ridges, but the discrete survey strategy makes it hard to evaluate the incidence of hydrothermal activity. On ultraslow-spreading ridges, fewer NTD-related hydrothermal activities were reported. Factors contributing to the occurrence of hydrothermal activities at NTDs and whether they could be potential targets for hydrothermal exploration are poorly known. Combining turbidity and oxidation reduction potential (ORP) sensors with a near-bottom camera, Chinese Dayang cruises from 2014 to 2018 have conducted systematic towed surveys for hydrothermal activity around a large NTD along the ultraslow-spreading Southwest Indian Ridge (SWIR, 48.1–48.7° E). Five new potential hydrothermal anomaly sites (2 inferred and 3 suspected) of high or low temperature and the previously inferred Sudi hydrothermal field occurred in diverse morphotectonic settings along a 78 km long ridge axis. The calculated vent frequency (Fs, sites/100 km) was ~7.7 over the entire study area, higher than the modified value (Fs ≈ 6.5) between 48 and 52° E of SWIR. Even only for the 54 km long large NTD, three hydrothermal anomaly sites yielded an Fs of ~5.6, which is higher than that of most ridge sections and is comparable to some fast-spreading ridges with high-resolution surveys. This indicates that NTDs along ultraslow-spreading ridges could be promising areas in fertilizing hydrothermal activities. Moreover, the deeply penetrating faults on the rift valley walls and strain-focused areas may contribute to the formation of NTD-related hydrothermal circulations. We suggest that NTDs along ultraslow-spreading ridges may be potential targets for further exploration of hydrothermal activities and seafloor sulfide deposits. Full article

Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen that causes periodic outbreaks of abortion in ruminant species and hemorrhagic disease in humans in sub-Saharan Africa. These outbreaks have a significant impact on veterinary and public health. Its introduction to the Arabian Peninsula in 2003 raised concerns of further spread of this transboundary pathogen to non-endemic areas. These concerns are supported by the presence of competent vectors in many non-endemic countries. There is no licensed RVF vaccine available for humans and only a conditionally licensed veterinary vaccine available in the United States. Currently employed modified live attenuated virus vaccines in endemic countries lack the ability for differentiating infected from vaccinated animals (DIVA). Previously, the efficacy of a recombinant subunit vaccine based on the RVFV Gn and Gc glycoproteins, derived from the 1977 human RVFV isolate ZH548, was demonstrated in sheep. In the current study, cattle were vaccinated subcutaneously with the Gn only, or Gn and Gc combined, with either one or two doses of the vaccine and then subjected to heterologous virus challenge with the virulent Kenya-128B-15 RVFV strain, isolated from Aedes mosquitoes in 2006. The elicited immune responses by some vaccine formulations (one or two vaccinations) conferred complete protection from RVF within 35 days after the first vaccination. Vaccines given 35 days prior to RVFV challenge prevented viremia, fever and RVFV-associated histopathological lesions. This study indicates that a recombinant RVFV glycoprotein-based subunit vaccine platform is able to prevent and control RVFV infections in target animals. Full article

Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus that causes an important disease in ruminants, with great economic losses. The infection can be also transmitted to humans; therefore, it is considered a major threat to both human and animal health. In a previous work, we described a novel RVFV variant selected in cell culture in the presence of the antiviral agent favipiravir that was highly attenuated in vivo. This variant displayed 24 amino acid substitutions in different viral proteins when compared to its parental viral strain, two of them located in the NSs protein that is known to be the major virulence factor of RVFV. By means of a reverse genetics system, in this work we have analyzed the effect that one of these substitutions, P82L, has in viral attenuation in vivo. Rescued viruses carrying this single amino acid change were clearly attenuated in BALB/c mice while their growth in an interferon (IFN)-competent cell line as well as the production of interferon beta (IFN-β) did not seem to be affected. However, the pattern of nuclear NSs accumulation was modified in cells infected with the mutant viruses. These results highlight the key role of the NSs protein in the modulation of viral infectivity. Full article

Compared to free antigens, antigens immobilized on scaffolds, such as nanoparticles, generally show improved immunogenicity. Conventionally, antigens are conjugated to scaffolds through genetic fusion or chemical conjugation, which may result in impaired assembly or heterogeneous binding and orientation of the antigens. By combining two emerging technologies—i.e., self-assembling multimeric protein scaffold particles (MPSPs) and bacterial superglue—these shortcomings can be overcome and antigens can be bound on particles in their native conformation. In the present work, we assessed whether this technology could improve the immunogenicity of a candidate subunit vaccine against the zoonotic Rift Valley fever virus (RVFV). For this, the head domain of glycoprotein Gn, a known target of neutralizing antibodies, was coupled on various MPSPs to further assess immunogenicity and efficacy in vivo. The results showed that the Gn head domain, when bound to the lumazine synthase-based MPSP, reduced mortality in a lethal mouse model and protected lambs, the most susceptible RVFV target animals, from viremia and clinical signs after immunization. Furthermore, the same subunit coupled to two other MPSPs (Geobacillus stearothermophilus E2 or a modified KDPG Aldolase) provided full protection in lambs as well. Full article

Well-crafted and scientifically accurate videos and animations can be effective ways to teach dynamic Earth processes such as continental rifting, both in live course offerings as well as in online settings. However, a quick search of the internet reveals too few high-quality videos/animations describing deep Earth processes. We have modified a hybrid 10.5 min video/animation about continental rifting and the formation of new oceans and passive continental margins created for an upper-division geology audience, retailoring it for a lower-division geology audience. A key challenge in successfully modifying such resources is aligning the cognitive load that the video/animation imposes on students, in part related to the technical geoscientific jargon used in explaining such phenomena, with that which they encounter on these topics in their textbooks and classrooms. We used expert feedback obtained at a 2019 GeoPRISMS (Geodynamic Processes at Rifting and Subducting Margins) workshop in San Antonio to ensure the accuracy of the science content of the upper-division video. We followed this with a review of the terminology and language used in the video/animation, seeking to align the video narrative with the technical language used in introductory geology offerings, which we based on examining five current introductory geology textbooks and feedback from students in introductory geoscience courses. The revised introductory-level video/animation was piloted in an online introductory course, where it provided an improved conceptual understanding of the related processes of continental rifting, opening new oceans, and formation of passive continental margins. Full article