Search Results (329)

Background and Objectives: To characterize the epidemiological profile of patients undergoing revision total hip arthroplasty (THA) using bone allografts from a tissue bank, and to identify clinical and surgical factors associated with the selection of graft type in cases of severe periprosthetic bone loss. Materials and Methods: This observational, cross-sectional study involved a retrospective review of medical records from a specialized referral center, including revision THA procedures performed between 2013 and 2019. Data were collected on 36 variables covering demographic details (age, sex), surgical history of both hips, comorbidities, medication use, perioperative complications, hospitalization, surgical technique, and characteristics of the bone grafts used. Patients were grouped based on the type of allograft received—structured or morselized (impacted)—and comparative analyses were performed. Results: A total of 67 revision THA cases were evaluated, with a mean patient age of 63.2 years. Nearly half (47.8%) had no prior hip revision. The average number of previous procedures per patient was 1.73, and the mean interval from primary THA to revision was 178.4 months. Morselized bone allografts were used in 66.7% of cases, and structured allografts in 33.3%. Patients receiving structured grafts had undergone a significantly higher number of prior surgeries (p = 0.01) and had a longer duration since the initial THA (p = 0.04). Conclusions: These findings suggest that younger patients undergoing primary total hip arthroplasty may be at increased risk for complex revision procedures involving structured grafts later in life, underscoring the need for long-term monitoring and tailored surgical planning in this population. Full article

Amid the ongoing digital transformation of higher education, increasing attention has been paid to the impact of digital technologies on teaching quality—particularly in physical education settings that require high levels of interaction and physical engagement. This study examined 1158 university students across China using a cross-sectional design, integrating interpretable machine learning models with structural equation modeling to systematically assess how the frequency of use of seven common digital technologies influences teaching quality in physical education classes. The study also investigated the mediating roles of perceived teacher support and academic self-efficacy. Nine machine learning models and logistic regression analyses were conducted to predict teaching quality, employing feature permutation importance and SHapley Additive exPlanations (SHAP) to evaluate the relative contribution of each digital tool. Results identified multimedia courseware, e-textbooks, and question banks as the most influential predictors of teaching quality. The SHAP analysis further revealed that management software, educational websites, and multimedia materials had significant positive effects and exhibited synergistic interactions in improving teaching outcomes. Structural equation modeling showed that digital technology use had a significant direct positive effect on teaching quality (B = 0.347, p < 0.001). Moreover, perceived teacher support and academic self-efficacy not only served as independent mediators (95% CI = [0.146, 0.226], p < 0.001; 95% CI = [0.024, 0.063], p < 0.001) but also functioned as a chain mediating effect (95% CI = [0.036, 0.083], p < 0.001). This study is the first to integrate interpretable machine learning with structural modeling to elucidate the mechanisms through which digital technologies influence teaching quality in university-level physical education. The findings underscore the critical mediating roles of teacher support and student self-efficacy, providing both theoretical contributions and practical implications for enhancing digital pedagogy in higher education. Full article

Background/Objectives: Although the nanocomposite of poly(L-lactic acid) with graphene oxide (PLLA-GO) shows promise for tissue engineering, its specific bioactive interactions with diverse cell lineages during early tissue regeneration remain unclear. This study comprehensively investigated the in vitro multifaceted biocompatibility of PLLA-GO using human fibroblasts (FN1 cells), murine mesenchymal stem cells (mBMSCs), and human umbilical vein endothelial cells (HUVECs). Methods: Morphological analyses were performed using optical and scanning electron microscopy, while proliferation dynamics were assessed via CFSE staining. Cell cycle progression was evaluated using flow cytometry, mitochondrial activity was examined through TMRE staining, and inflammatory cytokine profiling was performed via Cytometric Bead Array (CBA). Results: PLLA-GO exhibited primary biocompatibility across all evaluated cell lines, characterized by efficient adhesion and proliferation. However, significant cell-type-dependent modulations were observed. The FN1 cells exhibited proliferative adaptation but induced accelerated scaffold degradation, as evidenced by a substantial increase in cellular debris (5.93% control vs. 34.38% PLLA-GO; p = 0.03). mBMSCs showed a transient initial proliferative response and a significant 21.66% increase in TNF-α production (179.67 pg/mL vs. 147.68 pg/mL in control; p = 0.03). HUVECs demonstrated heightened mitochondrial sensitivity, exhibiting a 32.19% reduction in mitochondrial electrical potential (97.07% control vs. 65.82% PLLA-GO; p ≤ 0.05), alongside reductions in pro-inflammatory cytokines TNF-α (8.73%) and IL-6 (12.47%). Conclusions: The PLLA-GO processing method is crucial for its properties and subsequent cellular interactions. Therefore, rigorous and specific preclinical evaluations—considering both cellular contexts and fabrication—are indispensable to ensure the safety and therapeutic potential of PLLA-GO in tissue engineering and regenerative medicine. Full article

Through laboratory experiments in an S-shaped channel, this study analyzes how the flow Froude number, the ratio of ice-to-flow rate, pier spacing-diameter ratio, and bed material median grain size influence scour depth around side-by-side double piers under ice-jammed flow conditions. Unlike the development of a scour hole around a bridge pier in a straight channel, where the scour depth increases with the flow Froude number under ice-covered conditions, this study reveals that in an S-shaped channel, scour depth increases with the flow Froude number near the convex bank pier and decreases near the concave bank counterpart. Irrespective of ice conditions, a higher ratio of pier spacing-diameter correlates with augmented scour depth at the convex bank and diminished scour at the concave bank. As the ice-to-flow rate ratio increases, the ice jam thickness in the S-shaped channel also increases, leading to a significant decrease in the flow area and resulting in deeper scour holes around the piers. Equations have been developed to calculate the maximum scour depth around side-by-side double piers positioned in an S-shaped channel with ice-jammed flow. Full article

Estimating bed load in rivers is a critical aspect of river engineering. Numerous methods have been developed to quantify bed load transport, often yielding varying results depending on the bed surface texture and grain size. This study aims to investigate how vegetation on channel banks and bed material particle size influence bed load transport, bed shear stress, velocity distribution, and the Shields parameter. It also examines the impact of geometric changes in the channel cross-section on bed load transport capacity. To address these objectives, a novel simulation method was developed to analyze the effects of vegetated banks, bed material size, and channel geometry. Field investigations were carried out in two reaches of the Taleghan River in Iran—one with vegetated banks and one without. Complementary flume experiments were conducted at two scales, incorporating vegetation on the sidewalls. Results showed that Shields parameter distribution corresponded with bed load distribution across cross-sections. Increase in flow rate and the Shields parameter led to higher bedload transport rates. Near vegetated banks, flow velocity, shear stress, and bedload transport were significantly reduced, with velocity profiles showing distinct variations compared to non-vegetated sections. Full article

Our knowledge of the molecular data on viruses infecting potato (Solanum tuberosum) and its weeds in Indonesia still needs to be expanded. Thirteen potato leaves, together with one carrot (Daucus carota subsp. sativus) and one Oxalis latifolia leaves grew adjacent to potato fields were sampled in West, Central, and East Java Provinces of Indonesia to be tested using RT-PCR with universal primers to monitor the presence of potyviruses, carlaviruses, and begomoviruses in the surveyed regions. According to the test results and BLAST nucleotide analysis, four potato samples were found to be infected by potato virus Y (PVY, Potyvirus). All samples tested negative for carlaviruses and begomoviruses. Both phylogenetic trees constructed by MEGA11 software based on partial coat protein and partial coat protein + 3′-UTR sequences demonstrated that the four new PVY isolates belong to strain N. Nucleotide and amino acid homology analysis determined that all four new and 12 other strain N isolates registered in NBCI GenBank were identical at the observed genome region, suggested the same origin of Indonesian isolates. This study improved our understanding of the variation in PVY isolates in Indonesia and highlighted the need for tight surveillance since the country imports most of its tubers used as planting material. Full article

Transparency in ESG financial materiality disclosure by corporations is now in doubt due to the inconsistent ESG framework that governs ESG disclosures, particularly in developing nations like South Africa. This is evident in the financial performance of banks and manufacturing firms as a result of the higher rate of susceptibility to ESG issues. Hence, this study empirically investigated the effect of ESG financial materiality disclosure on the financial performance of banks and manufacturing firms in South Africa from 2015 to 2024. Also, the moderating role of ESG transparency on the relationship between ESG financial materiality disclosure and financial performance was investigated. Descriptive analysis, a correlation matrix, and panel regression analysis were employed for analysis purposes. The financial metrics include ROA, ROE, and Tobin’s Q, while ESG financial materiality disclosure and the ESG disclosure score of the firms were the independent variable and moderating variable, respectively. The results show that ESG financial materiality exerts a significant adverse impact on ROA and ROE but an insignificant positive effect on Tobin’s Q in banks. For manufacturing firms, the impact is insignificant and negative on ROA, ROE, and Tobin’s Q. Also, the interactive effect of transparency insignificantly weakens the effect of ESG financial materiality disclosure on financial performance in both banks and manufacturing firms. This concludes that the transparency in ESG financial materiality disclosure is not sufficient to improve financial performance in both sectors but should be integrated in the core business objectives of firms. Also, it suggests that over-disclosure and greenwashing of ESG reports should be avoided. Full article

Considering the taxonomic uncertainties of Neotropical deer species, as well as the threat status of many of them, new studies and strategies for their maintenance are urgently needed. Obtaining live cells is of great importance for the conservation of wild species in order to allow cytogenetic and molecular studies to be carried out and for the construction of genomic resource banks. In order to increase the genetic diversity stored in these banks, the possibility of collecting skin fragments from dead animals (e.g., run over, hunted, deaths related to disease or natural causes) becomes a valuable source and a last alternative for obtaining material from these individuals. However, the interval between the death of the animal and the collection of tissue can directly interfere with the quality of the sample obtained and it is therefore essential to identify the maximum time during which viable cells are still found. Thus, this study sought to establish a protocol for the collection, storage, cryopreservation, and cultivation of skin obtained postmortem from individuals of the species Subulo gouazoubira (gray brocket deer) and Mazama rufa (red brocket deer). The collection of tissue fragments at different postmortem intervals (0 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, and 11 h) was evaluated. The tissues were analyzed for fibroblast cell viability immediately after collection. Their ability to undergo cryopreservation was evaluated based on techniques that can be directly applied to samples obtained in the field and their subsequent thawing and success of cell cultures was performed in the laboratory. Regarding the genetic integrity of the cells, the number of metaphases was observed by the mitotic index. The cell viability presented by the samples always remained above 60%. It was possible to establish cell cultures even with the tissues obtained 11 h after the death of the individuals; however, they required twice as many days to reach bottle confluence compared to the cultures performed with the tissues obtained 0 h after the death of the individuals. The results suggest that the best rates of cell viability, time to reach confluence, and number of metaphases per cell (mitotic index) are found in skin fragments collected up to 5 h after the death of individuals when their carcasses are kept at room temperature. Full article

The genus Musa L. is one of the most important genera worldwide due to its use in food as a source of carbohydrates. A morphological characterization was performed to evaluate the potential of 100 accessions of Musa spp. from the Amazon region of Ecuador, applying 73 qualitative and quantitative descriptors in addition to the ecogeographic characterization. The multivariate analyses identified four large groups: The first is composed of the Musa AAB Simmonds ecotype “Hartón Plantain” and the “Cuerno Clone”. The second group is composed of the Musa acuminata Colla ecotype “Orito”. The third group is composed of the Musa acuminata ecotype “Malay plantain or red plantain”; and the fourth group is composed of the Musa × paradisiaca L. AAB ecotype “Barraganete” and banana or banana materials and the Musa AAB Simmonds ecotype “Plátano Dominico”. The qualitative descriptors with the highest discriminant value were the shape of the ♂ floret bud, the appearance of the rachis, and the pigmentation of the compound tepal, and the quantitative discriminant characters were the height of the pseudostem, the length of the leaf blade, the width of the leaf blade, and the weight of the raceme. The analysis with CAPFITOGEN of these 100 accessions through the ecogeographic characterization map identified 23 categories, highlighting category 20 with a coverage of 40.35%, which mainly includes the provinces of Orellana, Sucumbíos, part of Napo, Pastaza, and Morona Santiago. This category occurs within an annual temperature range between 21.6 °C and 27 °C, an apparent density of 1.25 to 1.44 g cm⁻³, and a cation exchange capacity (CEC) of 4 to 29 Cmol kg⁻¹. The morphological characterization of 100 Musa accessions revealed significant phenotypic variability, with four distinct morphological groups identified through cluster analysis. Key differences were observed in traits such as bunch weight, fruit length, and vegetative vigor. This variability highlights the potential of certain accessions for use in genetic improvement programs. The findings contribute valuable information for the efficient conservation, selection, and utilization of the Musa germplasm in Ecuadorian agroecosystems. The results demonstrate the existence of an important genetic variability in the INIAP Musa Germplasm Bank in the Ecuadorian Amazon region. Full article

The growth in the adoption of circular economy principles in the construction industry has given rise to material passports as a critical implementation tool. Given the existing problems of high resource use and high waste generation in the construction industry, there is a pressing need to adopt novel strategies and tools to mitigate the adverse impacts of the built environment. However, research on the application of material passports in the context of construction waste management remains limited. The aim of this paper is to identify the contextual uses, stakeholders, requirements, and challenges in the application of material passports for managing waste generated from building construction and demolition processes through a systematic review approach. Comprehensive searches in Scopus and the Web of Science databases are used to identify relevant papers and reduce the risk of selection bias. Thirty-five (35) papers are identified and included in the review. The identified key contexts of use included buildings and cities as material banks, waste management and trading, and integrated digital technologies. Asset owners, waste management operators, construction and deconstruction teams, technology providers, and regulatory and sustainability teams are identified as key stakeholders. Data requirements related to material, components, building stock data, lifecycle, environmental impact data, and deconstruction and handling data are critical. Moreover, the key infrastructure requirements include modeling and analytical tools, collaborative information exchange systems, sensory tracking tools, and digital and physical storage hubs. However, challenges with data management, costs, process standardization, technology, stakeholder collaboration, market demand, and supply chain logistics still limit the implementation. Therefore, it is recommended that future research be directed towards certification and standardization protocols, automation, artificial intelligence tools, economic viability, market trading, and innovative end-use products. Full article

Background and Objectives: Malaria is a disease that can result in a variety of complications. Diagnosis is carried out by an optical microscope and depends on operator experience. The use of artificial intelligence to identify morphological patterns in erythrocytes would improve our diagnostic capability. The object of this study was therefore to establish computer viewing models able to classify blood cells infected with Plasmodium spp. to support malaria diagnosis by optical microscope. Materials and Methods: A total of 27,558 images of human blood sample extensions were obtained from a public data bank for analysis; half were of parasite-infected red cells (n = 13,779), and the other half were of uninfected erythrocytes (n = 13,779). Six models (five machine learning algorithms and one pre-trained for a convolutional neural network) were assessed, and the performance of each was measured using metrics like accuracy (A), precision (P), recall, F1 score, and area under the curve (AUC). Results: The model with the best performance was VGG-19, with an AUC of 98%, accuracy of 93%, precision of 92%, recall of 94%, and F1 score of 93%. Conclusions: Based on the results, we propose a convolutional neural network model (VGG-19) for malaria diagnosis that can be applied in low-complexity laboratories thanks to its ease of implementation and high predictive performance. Full article

The loss of plant species, especially endangered and endemic ones, poses a significant threat to global biodiversity. These species cannot easily be replaced when their populations decline or become extinct, which makes their loss particularly devastating. This study focuses on the geobotanical study of nine Rosaceae species (Cotoneaster karatavicus, Crataegus ambigua, Malus niedzwetzkyana, Malus sieversii, Prunus tenella, Prunus ulmifolia, Sibiraea laevigata, Sorbus persica, and Spiraeanthus schrenkianus) and the development of ex situ approaches for the conservation of Rosaceae species listed in the Red Book of Kazakhstan. The geobotanical study revealed an alarming trend of biodiversity loss in five regions of Kazakhstan. This loss is driven by threats from diseases and pests, as well as the aging of plants, small population sizes, weak in situ fruiting, and other factors, such as climate change. We have established an in vitro collection for the short- and medium-term conservation of seeds, embryos and shoots taken either directly from field-grown plants or from budwood cuttings forced indoors. We also use long-term sexual conservation methods, such as the cryopreservation of seed and embryonic axes, alongside conventional seed banking at −20 °C. Ex situ conservation efforts that use multiple propagules and storage methods for the same species are well-suited to a diverse genebank facility. These efforts enable future generations to use this valuable reservoir of genetic diversity for crop improvement and may also serve as a basis for propagating planting material to restore degraded populations. Full article

Metaphors help to simplify complex concepts, making them more accessible and understandable for children. Children can build a more concrete understanding of these concepts by associating abstract financial ideas with familiar situations or objects. The present study aims to explore what children with above-average abilities understand by financial literacy, using words and images as tools of expression. During a workshop, 22 children with above-average abilities participated in two tasks, one individual and one group task. The results showed that “save” (90.9%), “money” (63.9%), “invest” (59.1%), and “bank” (54.5%) are the words most strongly associated with the concept of financial literacy among the children. Regarding images, money (M = 1.77), a clock or calendar (M = 2.50), a pig (M = 2.75), and a house (M = 2.84) were identified as the most representative symbols of financial literacy for this group of children. In the group task, children perceive financial literacy mainly as managing and using money to satisfy needs and desires. The results can inform educators about the need to adapt educational materials to match children’s level of understanding better, promoting more effective and accessible financial education. Full article

In recent years, the successful application of gravity-flow deposit theory in major petroliferous basins in China had attracted extensive attention in the field of sedimentology and had become a key research frontier. This study utilized core, drilling, logging, and microphotograph data, along with low-temperature nitrogen adsorption and high-pressure mercury injection experiments. It discussed the characteristics of gravity-flow deposits, sedimentary microfacies, sedimentary models, and the significance of gravity-flow deposits to pore heterogeneity in shale reservoirs, focusing on the first submember of the Dongyuemiao Member (referred to as the Dong 1 Member) in the Fuling area of the Sichuan Basin. The results indicated the development of four types of mudrock in the Dong 1 Member: massive to planar laminated shell mudrock (F1), planar laminated bioclastic mudrock (F2), planar laminated silty mudrock (F3), and massive mudrock (F4). These corresponded to debris flow deposits (F1, F2), turbidite deposits (F3), and suspension deposits (F4). According to the characteristics of lithofacies combinations and sedimentary features, four sedimentary microfacies were identified: gravity-flow channel, tongue-shaped, lobate, and semi-deep lake mud. The Shell Banks were disturbed by earthquakes, tides, storms, and other activities. Silt, clay, fossil fragments, plant debris, and other materials were deposited under the influence of gravity, mixing with surrounding water to form an unbalanced and unstable fluid. When pore pressure exceeded viscous resistance, the mixed fluid became unbalanced, and gravity flow began to migrate from the slope to the center of the lake basin. A sedimentary unit of gravity-flow channel-tongue-shaped-lobate was developed in the Fuling area. The Fuling area’s gravity-flow depositional system resulted in distinct microfacies within the Dongyuemiao Member, each exhibiting characteristic lithofacies associations. Notably, lobate deposits preferentially developed lithofacies F3, which is distinguished by significantly higher clay mineral content (60.8–69.1 wt%) and elevated TOC levels (1.53–2.45 wt%). These reservoir properties demonstrate statistically significant positive correlations, with clay mineral content strongly influencing total pore volume and TOC content specifically enhancing mesopore development (2–50 nm pores). Consequently, the F3 lithofacies within lobe deposits emerges as the most prospective shale gas reservoir unit in the study area, combining optimal geochemical characteristics with favorable pore-structure attributes. Full article

Bone grafting is one of the most used surgical techniques to favor bone regeneration and repair in orthopedic procedures. Despite autografting continuing to be considered the gold standard, allogeneic bone tissues remain a viable alternative albeit in the last decades, only a few studies have been carried out to translate enhanced allogeneic bone grafts into clinical solutions. In this in vitro study, cortical allogeneic bone samples were coated with copper-doped bioactive glass 45S5 (Cu-BG) by means of the pulsed-laser deposition technique to combine the mechanical support and osteoconductive properties of human bone with the osteogenic and pro-angiogenic features of the bioactive material. Contact angle (CA), scanning electron microscopy (SEM), and atomic force microscopy (AFM) measurements were carried out to quantitatively compare the impact on the bone surface properties of the morphological changes induced by the presence of the coating. Specifically, the obtained results have shown a total absorption of the drop on the coated samples. The coating on the bone tissue surface consisted of a homogeneous Cu-BG background layer with micrometric grain-like aggregates on it—a morphological feature that can facilitate osteoblast adhesion and proliferation. Cytotoxicity and cell viability were carried out on Saos-2 osteoblast-like cells, demonstrating the biocompatibility of the novel composite bone tissue and the absence of cytotoxic residuals. Moreover, human bone marrow-derived mesenchymal stem cells (hBMSCs) were seeded on Cu-BG and not-coated (NC) samples to evaluate the bioactivity and their differentiation toward the osteogenic phenotype. Our findings showed the pro-osteogenic and pro-angiogenic potential of Cu-BG coatings, although dynamic changes were observed over time. At seven days, the Cu-BG samples exhibited significantly higher expressions of SP7, SPP1, and BGLAP genes, indicating an enhanced early osteogenic commitment. Moreover, VEGF expression was significantly increased in Cu-BG compared to the control. These results pave the way for the development of an innovative class of bone-based products distributed by tissue banks. Full article