Search Results (1,195)

SARS-CoV-2 undergoes frequent mutations that drive viral evolution and genomic diversity, influencing transmissibility, immune escape, and disease severity. In this study, we performed whole-genome sequencing on SARS-CoV-2 isolates from patients in New York City and identified several globally rare mutations across multiple viral lineages. The isolates analyzed for rare mutations belonged to three lineages: B.1.1.7 (Alpha), B.1.526 (Iota), and B.1.623. We identified 16 rare mutations (global incidence <1000) in non-structural protein genes, including nsp2, nsp3, nsp4, nsp6, nsp8, nsp13, nsp14, ORF7a, and ORF8. Three of these mutations—located in nsp2, nsp13, and ORF8—have been reported in fewer than 100 individuals worldwide. We also detected five rare mutations in structural proteins (S, M, and N), including two—one in M and one in N—previously reported in fewer than 100 cases globally. We present clinical profiles of three patients, each infected with genetically distinct viral isolates from the three lineages studied. Furthermore, we illustrate a local transmission chain inferred from unique mutation patterns identified in the Omicron genome. These findings underscore the importance of whole-genome sequencing for detecting rare mutations, tracking community spread, and identifying emerging variants with clinical and public health significance. Full article

This paper enhances the calibration procedure for pricing spread options with liquidity risk. The novelty is the use of Chebyshev interpolation to fit the prices.Numerical experiments reveal that the calibrated parameters are close to the ones obtained by a previous work. However, the fit obtained by this paper is superior as shown by our plots. Full article

Educators must understand current practices and gaps in knowledge regarding environmental sustainability in simulation education to reduce the environmental impact of plastic waste while still maintaining fidelity in simulation education. Therefore, a scoping review was conducted to answer the PICO question, “In healthcare institutions and hospitals, what are the environmentally sustainable practices that can be translated into simulation labs as best practice?” Fourteen studies were identified through a search of seven databases, critically appraised, and analyzed. Three key themes emerged: (1) the 5 R’s, (2) getting people motivated, and (3) larger external collaboration. These themes highlight practical strategies and motivational factors for sustainable practices. An expanded 5 R’s framework (reduce, reuse, recycle, research, and rethink) was introduced to guide a holistic approach. The literature highlights the importance of education, stakeholder engagement, and clearly defined standards as key drivers for motivating individuals and teams to engage in sustainable behaviors. These efforts are most effective when supported by interdisciplinary collaboration, regulatory frameworks, national policies, and technological innovation. Sustainability initiatives should extend beyond individual institutions to foster broader systemic change. Full article

The formation of hot tearing during direct chill casting of aluminum alloys, specifically AA6111, is a significant challenge in the production of ingots for industrial applications. This study investigates the role of hydrostatic pressure and tensile stress in the formation of hot tearing during direct chill casting of rectangular ingots. Combining experimental results and finite element modeling with ABAQUS/CAE 2022, the mechanical behavior of the semi-solid AA6111 alloy was analyzed under different cooling conditions. “Hot” (low water flow) and “Cold” (high water flow) conditions were the two types of cooling conditions that produced cracked and sound ingots, respectively. The outcomes indicate that high tensile stress and localized negative hydrostatic pressure in the hot condition are the main factors promoting the initiation and propagation of cracks in the mushy zone, whereas the improvement of the cooling conditions reduces these defects. Full article

Miami, Florida, renowned for its cultural richness and coastal beauty, also faces the concerning challenges created by urban heat islands (UHIs). As one of the hottest cities of the United States, Miami is facing escalating temperatures and threatening heat-related vulnerabilities due to urbanization and climate change. Our study addresses the critical issue of mapping and investigating UHIs in complex urban settings. This study leveraged Planet satellite data and Landsat data to conceptualize and develop appropriate mitigation strategies for UHIs in Miami. Utilizing the Planet satellite imagery and Landsat data, we conducted a combined study of land cover and land surface temperature variations within the city. This approach fuses remotely sensed data to identify the UHI hotspots. This study aims for dynamic approaches for UHI mitigation. This includes studying the status of green spaces present in the city, possible expansion of urban green spaces, the propagation of cool roof initiatives, and exploring the recent climatic trend of the city. The research revealed that built-up areas consistently showed higher land surface temperatures while zones with dense vegetation have lower surface temperatures, supporting the role of urban green spaces in surface temperature reduction. This research can also set a robust model for addressing UHIs in other cities facing rapid urbanization and experiencing mounting temperatures each passing year by helping in assessing LST, land cover, and related spectral indices as well. Full article

Traffic in large cities is increasing due to continuous urbanization, the construction of new housing complexes and the accompanying new street network. The growth of cities creates prerequisites for increasing the intensity of transport, pedestrian, and bicycle flows, especially during peak periods. To improve the conditions in which traffic flows, it is necessary to introduce an effective method for reducing delays that arise at intersections, especially those regulated by traffic light systems. One of the possible approaches to this is to coordinate the operation of traffic light systems. The main thing in this is to determine relatively accurate times for the movement of individual flows, for which adequate traffic models are needed. This article presents a model of the movement of transport flows when starting from the first intersection in a coordinated mode of operation of traffic light systems. This is of particular importance when determining the times of individual signals and, above all, has an impact on the moment for switching on the permitting signal at the next intersection. The presented model aims to provide an opportunity to determine accurate times of passage of vehicles through consecutive intersections that operate in a coordinated mode of traffic light systems. Full article

Pebble Bed Reactors (PBRs) represent a new generation of nuclear reactors. However, modeling TRi-structural ISOtropic (TRISO) fuel particles employed in PBRs presents a unique challenge in comparison to most conventional reactor designs. Rapid generation of different possible fuel particle configurations for Monte-Carlo simulations provides improved insights into the effects of particle distribution irregularities on the neutron economy. Defective pebbles could cause changes in the neutron flux in a nuclear reactor due to increased or decreased moderating effects. Different configurations of particle fuel also impact isotope production within the nuclear reactor. This study simulates several TRISO configurations representing limited capabilities of randomization algorithms, manufacturing defects configurations and/or special pebble design. All predictions are compared to an equivalent homogenized model used as baseline. The results show that the TRISO configuration has a non-negligible impact on the parameters under consideration. To explain these results, the ratio of the thermal flux of each model to the thermal flux of the homogeneous model is calculated. A clear pattern is observed in the data: as irregularities in the moderator medium emerge due to the distribution of TRISO particles, the neutron spectrum softens, leading to higher values of k_∞ and better fuel utilization. This dependence of the spectrum on the TRISO configuration is used to explain the pattern observed in the depletion calculation. The results open the possibility of optimizing the TRISO configuration in manufactured pebbles for fuel utilization and safeguards. Future work should focus on full core simulations to determine the extent of these findings. Full article

The study of traffic parameters in cities is the basis for making adequate decisions related to the organization and regulation of traffic. This publication presents a study of one of the main parameters of transport flows, namely, its intensity. The study covers one of the busiest streets in the city of Sofia, which is part of the radial connection in the radial circular street network of the city, for the evening peak period of the day. Data analysis presents the influence of the intensity of transport flows at the intersections, which are formed by the intersection with other streets, on the load of the studied street. The share of the load of each transport flow at the individual intersections on the total load of the studied section was recorded for the subsequent assessment of the existing traffic management. The results have been provided to the relevant directorates in the structure of Sofia Municipality for information and use. Full article

Tourism offers many economic benefits but can have long-lasting ecological effects when improperly managed. Tourism can cause overwhelming pressure on wastewater treatment systems, as in Belize, where some of the over 400 small islands (cayes) that were once temporary sites for fishermen have become popular tourist destinations. An overabundance of nitrogen, in part as a result of incomplete wastewater treatment, threatens human health and ecosystem services. The tourism industry is a complex and dynamic industry with many sectors and stakeholders with conflicting goals. In this study, a systems thinking approach was adopted to study the dynamic interactions between stakeholders and the environment at Laughing Bird Caye National Park in Belize. The project centered on nutrient discharges from the caye’s onsite wastewater treatment system. An archetype analysis approach was applied to frame potential solutions to nutrient pollution and understand potential behaviors over time. “Out of control” and “Underachievement” were identified as system archetypes; “Shifting the Burden” and ‘‘Limits to Success’’ were used to model specific cases. Based on these results, upgrading of the wastewater treatment system should be performed concurrently with investments in the user experience of the toilets, education on the vulnerability of the treatment system and ecosystem, and controls on the number of daily tourists. Full article

The longstanding debate over early hominin subsistence strategies, particularly the hunting-versus-scavenging hypothesis, as well as discussions regarding the functionality of Oldowan sites, has been primarily centered on the archeological and paleoanthropological record of Olduvai Gorge. Historically, FLK Zinj has been at the core of these debates, serving as a principal empirical reference due to the prevailing assumption that most other Bed I sites at Olduvai represented non-anthropogenic accumulations However, recent discoveries have significantly reshaped this perspective. Newly identified early sites, including PTK, DS, and AGS, situated within the paleolandscape and thin stratigraphic context of FLK Zinj, provide crucial new anthropogenic datasets. These sites offer additional dimensions to the study of early hominin behavior, facilitating a more nuanced reconstruction of their adaptive strategies in this paleoenvironment. Furthermore, methodological advancements in recent years—including controlled experimental and actualistic studies, sophisticated statistical modeling, and the integration of machine learning algorithms—have greatly enhanced the analytical frameworks available for investigating early hominin behavior. These innovations have refined the ability to formulate and test hypotheses within a rigorous scientific paradigm, significantly improving the resolution of archeological and taphonomic interpretations. This study presents an in-depth taphonomic analysis of the faunal assemblage from level 22A at DS, a Bed I site at Olduvai Gorge dated to approximately 1.84 Ma. The assemblage exhibits exceptional preservation, enabling detailed assessments of skeletal part representation, fragmentation patterns, and surface modifications. By combining traditional taphonomic methodologies with state-of-the-art AI-driven bone surface modification (BSM) analyses, this research contributes novel insights into the interactions between early hominins and carnivores, elucidating the complex ecological dynamics of an Early Pleistocene African paleolandscape. Full article

Background/Objectives: Rates of receiving opioid use disorder (OUD) treatment among adolescents and young adults (AYA) aged 16–25 are low. The current study qualitatively analyzed informants’ perspectives regarding the availability of, developmental considerations relevant to, and barriers associated with OUD treatment for AYA. Methods: Thirty key informants involved with OUD treatment in the northeastern United States completed individual, semi-structured interviews, including treatment providers (N = 11) and clinic leaders in programs that provide medication and psychosocial treatments for AYA with OUD (N = 10), as well as opioid-related policymakers (N = 6) and patient advocates (N = 3). Interviews were transcribed and independently double coded. Template-style thematic analysis methods were used and revealed seven themes. Results: The first theme highlighted limited treatment program availability for adolescents (aged < 18 years) with OUD. Four themes related to developmentally optimizing OUD treatment for AYA, describing the importance of caregiver involvement, AYA peer connections, wraparound services, and early intervention. Two themes described barriers to AYA OUD treatment, including stigma and knowledge gaps about medications for OUD as well as deficits in AYA’s access to basic resources (e.g., housing, food security) that prohibit effective participation in treatment. Conclusions: Results highlight concerns from systems-level key informants regarding gaps in OUD treatment options for youth under the age of 18 and a high need for OUD treatment that is developmentally tailored to AYA. Findings point toward potential modifications and additions to existing adult treatment programs to make OUD treatment more accessible, relevant, and engaging for AYA. Full article

Urban blue–green space (UBGS) plays a critical role in mitigating the urban heat island (UHI) effect and reducing land surface temperatures (LSTs). However, existing research has not sufficiently explored the optimization of UBGS spatial configurations or their interactions with urban morphology. This study takes New York City as a case and systematically investigates small-scale urban cooling strategies by integrating multiple factors, including adjustments to the blue–green ratio, spatial layouts, vegetation composition, building density, building height, and layout typologies. We utilize multi-source geographic data, including LiDAR derived land cover, OpenStreetMap data, and building footprint data, together with LST data retrieved from Landsat imagery, to develop a prediction model based on generative adversarial networks (GANs). This model can rapidly generate visual LST predictions under various configuration scenarios. This study employs a combination of qualitative and quantitative metrics to evaluate the performance of different model stages, selecting the most accurate model as the final experimental framework. Furthermore, the experimental design strictly controls the study area and pixel allocation, combining manual and automated methods to ensure the comparability of different ratio configurations. The main findings indicate that a blue–green ratio of 3:7 maximizes cooling efficiency; a shrub-to-tree coverage ratio of 2:8 performs best, with tree-dominated configurations outperforming shrub-dominated ones; concentrated linear layouts achieve up to a 10.01% cooling effect; and taller buildings exhibit significantly stronger UBGS cooling performance, with super-tall areas achieving cooling effects approximately 31 percentage points higher than low-rise areas. Courtyard layouts enhance airflow and synergistic cooling effects, whereas compact designs limit the cooling potential of UBGS. This study proposes an innovative application of GANs to address a key research gap in the quantitative optimization of UBGS configurations and provides a methodological reference for sustainable microclimate planning at the neighborhood scale. Full article

Background: The Human Papillomavirus (HPV) vaccine generates high antibody titers against targeted HPV types. This study investigated vaccine-induced anti-HPV18 immunoglobulin (IgG) antibody titers and subsequent HPV18/45 infections. Methods: We performed a nested matched case-control study leveraging a prospective longitudinal cohort of adolescent and young adult women (AYW) vaccinated with the quadrivalent HPV vaccine (4vHPV) attending the Mount Sinai Adolescent Health Center (MSAHC) in Manhattan, NY. The case individuals included AYW who had an incident detection of cervical HPV18 (n = 3) or HPV45 (n = 34) DNA after vaccination and were compared to two vaccinated control individuals (HPV18/45-negative); one random control (RC, n = 37) and one high-risk control (HRC, n = 37) selected from the upper quartile of a sexual risk behavior score. Serological titers against HPV18 were measured by end-point dilution and enzyme-linked immunosorbent assay (ELISA) in serum collected before the incident detection of HPV. Matching was performed based on age at first dose, follow-up time, and sexual risk behavior score. Conditional logistic regression was used to assess the association between case-control status and anti-HPV antibody titers, consistent with the matched-pair design. Results: Antibody titers for HPV18 were most different between AYW who developed an HPV18/45 infection compared to high-risk controls OR = 1.66, 95% CI: 0.96–2.85 (p = 0.1629). Analyses of pooled data from vaccinated recipients including who developed HPV16/31 or HPV18/45 infections demonstrated that the odds of a one-log unit increase in anti-HPV16 or 18 antibody titers, respectively, were 40% higher in the combined control groups (RC + HRC, n = 160) (OR = 1.40, 95% CI: 1.09–1.79, p = 0.0135) and 73% higher in the HRC (n = 80) (OR 1.73, 95% CI: 1.34, 2.52, p = 0.0117) compared to HPV16/18/31/45 cases (n = 80). Conclusions: Overall, these findings suggest that higher IgG antibodies to HPV16/18 after vaccination represent an increased likelihood of protection from homologous and cross-reactive HPV types (HPV16/18/31/45). These results show that differences in antibody titers are associated with breakthrough infection after vaccination, suggesting that further study of long-term antibody titers and infection should be pursued. Full article

Background/Objectives: How to best assist people living with dementia (PLWD) and their care partners to maximize quality of life and quality of living, through appropriate and effective non-pharmaceutical approaches, remains a focus of dementia societies and organizations worldwide. This scoping review explored the types, opportunities, benefits and challenges of outdoor-based care and support programs for community-dwelling PLWD and their care partners. Methods: The methodological framework proposed by Arksey & O’Malley (2005) and modified by Levac et al. (2010) was followed. Four research databases were searched from January 2000 to November 2024; 2817 articles were retrieved and 20 met inclusion criteria and underwent data extraction. Results: Program types included the following: nature-based, care farm, green care farm, and farm-based dementia care programs, representing more than half of the included articles; health walks; and outdoor horticulture/garden/gardening programs. The number of PLWD participants ranged from 4 to 136. The total number of care partner participants was 151 and the total number of service provider participants was 87. The essence of, and connections with, nature and the outdoors had notable relevance and value for PLWD. Reported benefits, assessed quantitatively and qualitatively, were numerous and those in the social domain were reported most often. Identified challenges and barriers were related to risks and safety and program development, and implementation and delivery. Conclusions: The noteworthy potential of outdoor-based care and support programs to promote and enhance the quality of life and quality of living of community-dwelling PLWD and their care partners was elucidated. As most PLWD prefer to, and do, continue to reside in their homes, including those with advancing symptoms, the findings have significant relevance and implications for real-world practice and policy. Future research addressing current gaps will strengthen this growing field of dementia research. Full article

Miraruira is a medicinal plant-based product (MPBP) that is widely used in the state of Amazonas for the treatment of diabetes, though its botanical identity remains unclear, which raises concerns about authenticity and therapeutic consistency. One solution to this problem is the use of mass spectrometry-based approaches, which have emerged as powerful tools for verifying botanical origin based on chemical composition. Thus, to confirm the botanical authenticity of miraruira, direct-injection mass spectrometry (DI-MS) and chemometric analyses (PCA and HCA) were conducted on methanol fractions of Salacia impressifolia and Connarus ruber, both suspected sources of miraruira, as well as commercial samples obtained in street markets in Manaus, Brazil. Additionally, the hexane extracts of C. ruber and the commercial samples were screened for benzoquinones using DI-MS, as these compounds are recurrent in the genus Connarus. The DI-MS and PCA analyses revealed distinct chemical profiles for each species, and identified mangiferin and epicatechin as chemical markers for S. impressifolia and C. ruber, respectively. Furthermore, PCA demonstrated that all the commercial samples exhibited chemical profiles closely aligned with C. ruber. However, the HCA indicated variability among these samples, suggesting C. ruber or related Connarus species are the primary sources of miraruira. Moreover, embelin, rapanone, and suberonone were identified as the main compounds in the hexane extracts of C. ruber and the commercial products. This study successfully confirmed the botanical authenticity of miraruira, identified key bioactive compounds related to its traditional use in the treatment of diabetes symptoms, and demonstrated the effectiveness of DI-MS as a valuable tool for addressing authenticity issues in MPBPs. Full article