Search Results (1,746)

Acantholyda posticalis (Hymenoptera: Pamphiliidae) is a forestry pest in China. They primarily infest pine trees, causing serious ecological damage. The research aims to identify the key environmental factors influencing the suitable distribution area of Acantholyda posticalis and their optimal conditions, and investigate the impacts of climate change and possible impacts of its main host plants on the distribution of Acantholyda posticalis. By utilizing the MaxEnt model, we predict the potential distribution of Acantholyda posticalis and its main host plant, Pinus tabuliformis, under current and future climatic conditions. The results indicate that under current climatic conditions, the suitable areas for Acantholyda posticalis in China are extensive in the Loess Plateau and North China Plain regions and have extensive overlapping area with the distribution of Pinus tabuliformis. The dominant environmental factors influencing the distribution of suitable areas for Acantholyda posticalis are the Minimum Temperature of the Coldest Month, Precipitation of the Wettest Quarter, Altitude and Temperature Seasonality. Under the SSP126 and SSP585 climate scenarios for the period 2081–2100, the overall suitable area for Acantholyda posticalis is projected to follow a decreasing trend, exhibiting a tendency to extend toward the southern and eastern regions. Meanwhile, the moderately and highly suitable areas are more concentrated and extensive. The research provides a theoretical foundation for the control of Acantholyda posticalis and the protection of the ecological environment. Full article

Microbial necromass carbon (MNC) is recognized as an important and relatively stable component of soil organic carbon (SOC); however, it is often overlooked and poorly understood in soil management practices, particularly in the context of Loess Plateau farmlands. Here, a 13-year field experiment was carried out to examine the differences in MNC distribution, the role of MNC in SOC storage, and the impact of environmental factors under long-term mulching practices. The experiment used four treatments: (1) no mulching (NT), (2) straw mulching (NSM), (3) plastic mulching (NPM), and (4) ridge mulching (NRM). Compared to NT, all mulching methods increased SOC levels, phospholipid fatty acids (PLFAs), and amino sugar (AS) content. Straw mulching enhanced microbial biomass carbon (MBC), reduced the gap in AS content between rhizosphere and non-rhizosphere soils, and significantly increased MNC. Conversely, NPM and NRM primarily increased MBC and MNC within the rhizosphere soil. Generally, the rhizosphere soil had higher AS content than non-rhizosphere soil. However, regarding the proportion of MNC contributing to SOC, non-rhizosphere soil showed a significantly greater contribution than rhizosphere soil (p < 0.05). The contribution of MNC to SOC ranged from 10.70% to 26.38% under different treatments. Fungal-derived MNC generally contributed more to SOC (7.96–19.73%) than bacterial-derived MNC (2.62–6.65%). Soil temperature, the C/N ratio, pH, and total phosphorus influence microbial community structure and MBC, which in turn affect MNC and regulate SOC. These results enhance our understanding of how agricultural management practices on the Loess Plateau affect carbon sequestration. Full article

Traditional Chinese brick-and-stone archways are not merely architectural products shaped by geographical constraints; they also embody a highly rational structural logic. Drawing on the unique earthen environment of the Loess Plateau and the region’s traditions of brick-and-stone construction, the Jinzhong region of China has developed a distinct system of archways. Consequently, to deconstruct the mechanical wisdom inherent in the traditional building techniques of the Jinzhong region, this study selected residential buildings in Qi County and Pingyao, as well as Qing Dynasty (1636–1912 AD) official architecture, as case studies. Through field investigations into the masonry techniques of three typical vault forms—the single-centre arch, the double-centre arch, and the four-centre arch—the study revealed their evolutionary characteristics in terms of geometric form. Static numerical simulation analysis was conducted using the Abaqus CAE 2025 (Dassault Systèmes, Vélizy-Villacoublay, France) platform. The study found that, under a simulated surface load of 0.027 N/mm², different arch profiles exhibited significant quantitative mechanical differences, and their stress distributions and deformation thresholds showed distinct scenario-specific tendencies. The results show that, compared to a semicircular arch, the official double-centred arch reduces maximum displacement by approximately 20%, and the maximum principal stress decreased from 1.35 MPa to 1.215 MPa, effectively mitigating the risk of cracking at the arch crown. With this high sectional stiffness and displacement-constraining capability, it supports the high load requirements of defensive city fortifications. Compared to the Pingyao gentle-type four-centre arch, its maximum displacement increased by only about 10%, and the maximum principal stress rose by only about 8%. Therefore, given similar mechanical performance but considering construction feasibility, the official double-centred arch was selected for the construction of defensive city fortifications. Furthermore, although the stress concentration at the corners (arch feet) of the Pingyao gentle-curved four-centred arch is approximately 4.8% higher than that of the pointed four-centred arch, its spatial utilization is improved by 15–20%; This geometric trade-off achieved through composite curvature maximizes interior clear space while maintaining structural stability, aligning with the functional requirements of guyao architecture for large-span living spaces. Meanwhile, the semicircular vaults of Qi County demonstrate universal value in low-load residential door and window components due to their low construction threshold. These quantitative data and qualitative observations indicate that the evolution of traditional forms is not merely an esthetic pursuit, but rather a precise optimization of structural performance within the constraints of material strength. This coupled relationship between “geometric form, load-bearing mechanism and usage context” confirms the inherent principles of resource efficiency and performance balance within traditional building systems. The quantitative assessment framework established in this study provides scientific guidance, grounded in construction logic, for the preventive conservation and precise reinforcement strategies of historic masonry structures. Full article

As a typical mountain ecosystem in the western Tianshan Mountains, the Ili River Valley possesses abundant vegetation resources. Soil nematodes are effective biological indicators for evaluating soil micro-food webs. Nevertheless, the response mechanisms of nematode community structure to distinct vegetation types, especially native trees and forest-edge shrubs, remain poorly understood in this region. In this study, two dominant tree species (Picea schrenkiana and Malus sieversii) and two forest-edge shrub species (Berberis heteropoda and Berberis sibirica) were investigated. We analyzed the composition, diversity, and energy structure of rhizosphere soil nematodes and further compared their differences among plant species. The results indicated that tree rhizospheres had significantly higher amounts of nitrate nitrogen ($\mathrm{N}{\mathrm{O}}_3^{-}$-N and microbial biomass carbon (MBC), along with a lower amount of extractable organic carbon/extractable total nitrogen (EOC:ETN) than shrub rhizospheres (p < 0.05). Picea schrenkiana (PS) exhibited greater root carbon storage, higher root biomass, and a higher root carbon-to-nitrogen ratio (RC:RN) than Berberis heteropoda (BH) and Berberis sibirica (BS) (p < 0.05). The genus Chiloplacus dominated the nematode community across all four woody plants. The relative abundance of omnivore-predatory nematodes was markedly higher in shrubs (BH and BS) than in trees (PS and MS). The soil food webs of PS and MS were degraded, whereas shrub food webs were in a transitional state between structured and degraded habitats. Shrubs presented a higher maturity index, structural metabolic footprint, and energy flux of omnivore-predatory nematodes, but a lower energy flux of bacterivorous nematodes. Additionally, PS had the highest nematode carbon use efficiency (NCUE) and the lowest energy flux uniformity (U). $\mathrm{N}{\mathrm{O}}_3^{-}$-N extractable total nitrogen (ETN), soil organic carbon (SOC), and root traits were the primary factors driving variations in nematode communities and carbon indicators. Therefore, nematode carbon indicators closely associated with soil carbon and nitrogen cycling have the potential to serve as sensitive auxiliary biological metrics for evaluating material cycling and energy flow in pure forests and shrub ecosystems. This study provides empirical support for the assessment of regional ecosystem stability. Full article

This study comprehensively analyzed the compounded effects of climatic factors and non-climatic factors on vegetation dynamics in the northern Shaanxi Loess Plateau region in China. The objective was to provide robust scientific insights and a solid theoretical framework to support the long-term stability and sustainable development of the local ecosystem. The temperature vegetation dryness index was used to improve the water stress factor of the CASA model, so as to estimate the NPP of vegetation on the Loess Plateau of northern Shaanxi from 2000 to 2020. The temporal and spatial change characteristics of vegetation NPP and its relationship with climatic factors were analyzed using the coefficient of variation, the Mann–Kendall test of significance, and second-order partial correlation analysis. The partial derivative residual trend method was used to isolate the specific impacts of climatic factors and non-climatic factors on vegetation NPP. The results indicate the following: (1) The vegetation NPP shows a notable upward trend, with an annual growth rate of 9.4195 gC·m⁻²·a⁻¹ and a long-term average of 269.71 gC·m⁻², with the spatial distribution showing markedly high south, low north, and latitudinal zonation characteristics. (2) Vegetation NPP exhibits positive correlations with temperature, precipitation, and solar radiation. Among these factors, precipitation shows the strongest correlation with variations in vegetation NPP. (3) Non-climatic factors are the main factor affecting vegetation NPP across most parts of the study area, which is greater than the effect of selected climatic factors, and human activities may be the key component within non-climatic factors. Full article

Runoff on the Chinese Loess Plateau has declined substantially over recent decades, but the relative roles of climate change and non-climatic disturbance remain debated. Here, we provide a robust regional attribution of runoff reduction across 14 major catchments during 1961–2009 by integrating seven Budyko-based climate elasticity methods with long-term hydro-meteorological analysis and change-point detection. Across the region, runoff and runoff coefficients decreased markedly, while evapotranspiration and leaf area index increased, indicating a widespread reduction in catchment water yield. Runoff showed consistently greater sensitivity to precipitation than to potential evapotranspiration, highlighting precipitation as the primary climatic control on runoff variability. However, the Budyko-based climatic component explained only part of the observed runoff decline, and the residual component not explained by annual precipitation and potential evapotranspiration was large in many catchments, with estimated contributions generally exceeding 50% and reaching more than 80% in several basins. Independent evidence, including vegetation greening, the expansion of ecological engineering measures, and increasing anthropogenic water demand, suggests that this residual was at least partly associated with human disturbance, although other non-Budyko climatic and hydrological processes may also contribute. These results indicate that annual precipitation and potential evapotranspiration alone cannot explain runoff decline across much of the Loess Plateau and underscore the need to jointly consider climatic forcing, land surface alteration, and direct human water use in regional water management. Full article

To elucidate the mechanisms of nutrient cycling in rhizosphere soil and microbial metabolism during the prolonged continuous cropping of lavender, this study examined the rhizosphere soil of lavender with different continuous cropping years (1, 4, 7, 10, 15, and 20 years) in the Ili River Valley of Xinjiang, China, measuring physicochemical properties, microbial biomass C/N/P, and eight extracellular enzyme activities. Microbial carbon use efficiency (CUE) and nutrient limitation were quantified using vector analysis, threshold elemental ratios (TERs), and two derived indices (TER_EEA and TER_L). Soil properties exhibited distinct nonlinear patterns: SOC peaked at 4 years (p < 0.05), TN was highest at 20 years, and TP was lowest at 4–7 years. MBC and MBN peaked at 20 years, whereas MBP was significantly lower than in 1-, 4-, and 10-year fields (p < 0.05). EEC and EEN were highest at 20 years, while EEP was lowest at 4 years (p < 0.05). The activity of carbon-related acquisition enzymes increases from 134.81 μmol/g·h in the first year to 393.86 μmol/g·h in the 20th year, an increase of 192%; the activity of nitrogen acquisition enzymes increases from 686.11 μmol/g·h in the first year to 1430.58 μmol/g·h in the 20th year, an increase of 108%. This indicates that the decomposition of organic matter and the nutrient cycling capacity continue to enhance. Vector analysis showed a mean VA of 46° and VL of 0.25, with VA > 45° (P limitation) at 1–4 years shifting to VA < 45° (N limitation) at 20 years. Critically, TEREEA and TERL produced opposite dominant limitations due to differing normalization frameworks—TEREEA scales by microbial biomass stoichiometry—while TERL normalizes against enzyme-derived thresholds. CUET and CUEE ranged from 0.42 to 0.56, with the minimum at 10 years and relatively high values at 15–20 years (p < 0.05). RDA identified CBH (26.2%) and NO₃⁻–N (19.8%) as primary drivers, with extractable phosphorus exhibiting the strongest regulatory effect (pseudo-F = 26.0). These results demonstrate that multi-model stoichiometric assessment is essential, as single indices may yield contradictory diagnoses. These results demonstrate that multi-model stoichiometric assessment is essential, as single indices may yield contradictory diagnoses, and the observed nonlinear shifts in dominant limitation type provide a mechanistic basis for targeted nutrient management in sustainable lavender cultivation. Full article

Background/Objectives: This cross-sectional study aimed to estimate the Locally Estimated Scatterplot Smoothing (LOESS)-smoothed percentiles for growth trajectories and evaluate age-related tendencies across groups using visual cross-sectional graphs. Methods: A total of 1147 patient records were analyzed, including 648 females and 469 males aged 5–20 years, with a mean age of 11.9 (SD ± 3.8) years. Twenty-seven cephalometric variables were organized into six measurement domains: cranial base, maxillary complex, mandibular complex, occlusal plane, vertical relationship, and sagittal relationship. Percentile curves were generated using LOESS regression across an age range of 5–20 years. Results: The LOESS-smoothed curves showed age-related trends across age groups. An upward trend in the curves was observed for the anterior and posterior cranial bases between 5 and 12 years of age, a plateau indicating reduced age-related change across groups during mid-adolescence. Maxillary measurements showed a similar pattern, with a clear upward tendency during childhood and reduced age-related change after approximately 12 years. Mandibular length and projection showed increasing trends during childhood, followed by a plateau or reduced slope across later age groups. The occlusal plane and vertical dimensions showed consistent patterns that approached a plateau around 12 years, indicating minimal age-related differences between groups. Changes in the ANB angle and Wits appraisal reflected a progressive forward tendency of the mandible across childhood age groups, followed by reduced age-related change during adolescence. Conclusions: These findings suggest that many craniofacial measurements show an upward trend during childhood followed by a plateau or reduced age-related change across age groups between approximately 12 and 14 years. The percentile-based growth curves presented here offer a practical reference for clinicians to evaluate craniofacial growth trajectories as population-level approximations derived from cross-sectional data in the pediatric population. Full article

Against the backdrop of global climate change and the “carbon neutrality” target, the ecological quality improvement of the Loess Plateau—a key region for ecological restoration in China—and its impact on vegetation carbon sources hold significant importance for regional carbon balance and ecological security. Based on MODIS and meteorological reanalysis data from 2002 to 2024, this study constructed the Remote Sensing Ecological Index (RSEI). Combined with a carbon source/sink model, it systematically assessed the spatiotemporal coupling evolution characteristics of ecological environment quality and vegetation carbon storage capacity in the Loess Plateau, and explored the synergistic driving mechanisms of major hydrothermal and surface factors. The results indicate the following: (1) From 2002 to 2024, the ecological environment of the Loess Plateau improved significantly, with the RSEI rising from moderate to good. This improvement was accompanied by a marked decrease in surface dryness, an increase in surface wetness, and notable growth in vegetation cover, revealing a positive coupling relationship characterized by “reduced surface dryness—increased surface wetness—enhanced vegetation restoration.” (2) Regional vegetation carbon storage capacity strengthened markedly. Gross Primary Productivity (GPP), Net Primary Productivity (NPP), and Net Ecosystem Productivity (NEP) all showed significant increasing trends, and the proportion of area classified as carbon sink increased substantially. (3) Spatially, carbon sink distribution exhibited a pattern of “higher in the southeast, lower in the northwest.” Sub-regions A and D were identified as core areas with higher ecological quality and carbon sink capacity, whereas sub-regions B and C were more ecologically fragile and served as primary carbon source areas. (4) The implementation of soil and water conservation measures on the Loess Plateau has effectively enhanced regional carbon storage capacity. Vegetation restoration, improved water conditions, and reduced surface dryness have jointly driven the transition of the Loess Plateau ecosystem from a “vulnerable type” to a “recovering type”, while ecological restoration projects have played a certain role in enhancing the carbon sink. This study provides a theoretical basis and scientific–technological support for ecological protection and high-quality development in the Yellow River Basin. Full article

Soil erosion and nutrient loss degrade the soil resource base and water quality in dryland agricultural landscapes, yet optimal design of vegetation buffers for soil conservation under intensifying rainfall remains poorly quantified, particularly for nutrient retention. This study is novel in integrating event-scale rainfall-simulation experiments, Bayesian hierarchical modelling, Causal Forest analysis, and WEPP simulations to quantify how the sequential addition of biocrusts and grasses to shrub buffers shifts density thresholds for runoff, soil loss, and nutrient export across varying rainfall intensities. Experiments were conducted across a continuous shrub-density gradient (0–11,429 plants ha⁻¹) representing three configurations: shrub monoculture, shrub-biocrust, and shrub-biocrust-grass on agricultural hillslopes of the Chinese Loess Plateau. Runoff, soil loss, and exports of total nitrogen (TN) and total phosphorus (TP) were measured. Results demonstrate three main findings. First, multilayer shrub–biocrust–grass buffers exhibited lower soil loss than monocultures. Posterior estimates indicate reductions from approximately 3.8 t ha⁻¹ at moderate monoculture density to below 1.0 t ha⁻¹ at lower planting densities, with 94% of the highest-density intervals reflecting uncertainty in these estimates. Second, Causal Forest analysis reveals a functional separation of controls: rainfall intensity dominates soil loss (88% importance) and runoff (84%), whereas nutrient retention responds more strongly to buffer structure and density management. Third, WEPP simulations across rainfall intensities (50–180 mm h⁻¹) and slopes (10–30%) identify an optimal multilayer buffer density of 3800–5700 plants ha⁻¹, which delivers robust multifunctional benefits with 50–67% lower planting requirements than conventional high-density monocultures. These findings demonstrate that multilayer vegetation buffers enhance soil retention and reduce nitrogen and phosphorus losses from hillslopes, sustaining the soil resource base and protecting water quality in dryland agricultural landscapes. The integrated modelling framework provides transferable, evidence-based density recommendations for climate-resilient soil conservation in similar dryland regions. Full article

Accurate quantification of ecohydrological processes is essential for effective water and carbon management in terrestrial ecosystems. Traditional simulations mainly rely on mechanistic models, yet their accuracy is often limited by inconsistencies in representing physical processes and uncertainties in parameterization. Integrating remote sensing signals offers a promising way to reduce these uncertainties and enhance model applicability. In this study, in-situ observations from a wheat cropland in the Guanzhong Plain were used to simulate gross primary productivity (GPP) and latent heat flux (LE) by comparing a forward model (STEMMUS-SCOPE) with a remote sensing-driven inverse model (STEMMUS-MLR). We further examined the role of solar-induced chlorophyll fluorescence (SIF), an emerging proxy for photosynthesis, as an input to improve mechanistic modeling of GPP and LE. Results show that STEMMUS-MLR outperformed STEMMUS-SCOPE in estimating water and carbon fluxes, demonstrating that incorporating SIF effectively reduces bias associated with uncertainties in parameters and forcing data. The contribution of SIF was quantified using Random Forest regression and Shapley additive explanations (SHAP), revealing that SIF markedly reduced the dependence of GPP and LE simulations on shortwave radiation (SW), air temperature (Ta), and leaf area index (LAI). These findings highlight the critical role of SIF in ecohydrological modeling of semi-arid cropland ecosystems and provide a scientific basis for advancing process understanding and improving the precision management of water and carbon budgets in terrestrial ecosystems. Full article

Elevated temperatures due to global climate change adversely affect plant growth and development, which has become a major factor restricting wheat (Triticum aestivum L.) production. Despite the introduction of dwarfing genes that have enhanced lodging resistance as well as productive potential in wheat breeding, lodging still affects wheat yields. Plant growth regulators are widely recognized as effective agents in mitigating crop lodging. Few studies have investigated the high-temperature lodging sensitivity of wheat genotypes from different breeding periods, nor have they examined how uniconazole-sucrose regulates lodging resistance under heat stress. To fill this research gap, an experiment was conducted in which two contrasting wheat genotypes from different periods, Bima 1 (BM1, ~135 cm tall, a historical genotype released in 1953, lodging-susceptible) and Shannong 28 (S28, ~75 cm tall, a modern genotype released in 2014, lodging-resistant), were exposed to high temperature stress combined with uniconazole-sucrose application. The results showed that high-temperature-induced increases in plant gravity center height, together with decreased stem diameter coefficient, stem plumpness, and lignin deposition, were the main factors responsible for the reduction in bending section factor and mechanical strength of wheat stems. These modifications are associated with reduced lodging resistance, increased susceptibility to lodging, and significant yield losses. Nevertheless, exogenous application of uniconazole-sucrose lowers plant gravity center height, enhances stem diameter coefficient, stem plumpness, and lignin content, thus alleviating lodging risk and boosting wheat yield under high temperature stress. High temperature stress was associated with downregulated relative expression levels of key genes involved in lignin metabolism and reduced activities of the corresponding key enzymes, as well as inhibited lignin biosynthesis and accumulation in stems and increased incidence of wheat lodging. Conversely, foliar spraying of uniconazole-sucrose alleviated these suppressive effects on lignin biosynthesis, thus enhancing stem mechanical strength and reducing the lodging index of wheat. Moreover, these indicators were more sensitive to heat stress or uniconazole-sucrose treatment in BM1. The two genotypes examined suggested a potential trend that S28 may exhibit reduced sensitivity to high temperature in terms of mechanical traits and lignin synthesis, which could contribute to enhanced lodging resistance under heat stress. Full article

Assessing the construction suitability and sediment reduction potential of dry-farming wide terraces is critical for improving soil and water conservation in semi-arid and semi-humid regions, yet these aspects are seldom evaluated within an integrated framework. Focusing on the Loess Plateau, this study delineates potential construction areas based on precipitation constraints, quantifies soil erosion using the Revised Universal Soil Loss Equation, and develops a multidimensional framework to jointly evaluate construction suitability and sediment reduction potential on sloping farmland. Results indicate that slope, transportation accessibility, and soil erosion intensity are the primary determinants of suitability. Highly suitable, suitable, and marginally suitable areas account for 7.5%, 7.2%, and 4.3% of the study area, respectively, with Shanxi, Shaanxi, and Gansu provinces—and particularly Yulin, Yan’an, and Qingyang—emerging as priority regions for implementation. Scenario analysis suggests that targeting (i) highly suitable and suitable areas or (ii) all suitable classes would reclaim approximately 59.89 × 10³ km² and 77.19 × 10³ km² of sloping farmland, respectively, leading to reductions in mean soil erosion modulus of 16.6% and 22%. These findings provide a quantitative basis for optimizing terrace deployment and advancing regionally targeted soil erosion mitigation strategies on the Loess Plateau. Full article

Under the background of the global “polycrisis”, enhancing ecological resilience has become a key pathway for achieving sustainable urban development, particularly in ecologically fragile regions. This study takes the Loess Plateau as the study area and constructs an evaluation framework for urban ecological resilience based on three dimensions: resistance, adaptability, and recovery. By integrating multi-source data from 1990 to 2020, the spatiotemporal evolution of urban ecological resilience in the region is analyzed, and the Geodetector is employed to identify the impacts of natural, economic, and social factors on urban ecological resilience. The main findings are as follows: (1) The urban ecological resilience index on the Loess Plateau shows an overall trend of initial increase followed by decline. Cities in both the western and eastern parts exhibit similar evolutionary patterns, while cities in the central region demonstrate a continuous upward trend, with the resilience index increasing from 0.3658 in 1990 to 0.3838 in 2020. (2) Urban ecological resilience in the study area exhibits a spatial gradient pattern characterized by a decrease from core cities toward peripheral areas. Resilience levels gradually weaken outward from urban centers. The spatial agglomeration effect is relatively weak, with most regions showing insignificant clustering and lacking contiguous high-resilience clusters. (3) Urban ecological resilience on the Loess Plateau is jointly influenced by multiple factors, with the effects of key drivers displaying clear stage-specific characteristics. Socioeconomic factors—particularly population density, per capita GDP, and industrial structure—serve as the primary driving forces, while natural conditions provide basic constraints. Interactions among factors are dominated by bivariate interactions and nonlinear synergistic effects. These findings deepen the understanding of the evolution and driving mechanisms of urban ecological resilience in ecologically fragile regions and provide scientific support for enhancing urban resilience and promoting sustainable development in the Loess Plateau. Full article

Pathogen manipulation of host behavior is a widespread evolutionary strategy to enhance its transmission, yet whether different pathogens elicit distinct behavioral and molecular responses in the same host remains poorly understood. We performed parallel behavioral assays and comparative transcriptomic analyses on third-instar Lymantria dispar larvae infected with Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV, virus), Staphylococcus aureus (bacterium) and Metarhizium anisopliae (fungus). Climbing height was recorded over 72 h post-infection, and gene expression pattern was profiled using RNA-seq at 72 h. Only LdMNPV infection induced significant, sustained upward climbing behavior among the three pathogen infection groups. All three pathogens activated Toll and IMD immune pathways, but LdMNPV triggered substantially broader transcriptomic reprogramming. Notably, the virus specifically upregulated multiple energy metabolism pathways (nicotinate/nicotinamide metabolism, pyruvate metabolism, TCA cycle and oxidative phosphorylation) and the neuroactive ligand-receptor interaction pathway—a pattern absent in bacterial and fungal infections. LdMNPV drove tree-top disease through a virus-specific, multi-system manipulation strategy that couples metabolic activation with neural signaling modulation. This comparative study reveals fundamental differences in behavioral manipulation across pathogen kingdoms and provides candidate pathways for functional validation. Full article