Search Results (160)

Aerogel sponges of bio-based polymers loaded with metal–organic frameworks (MOFs) are highly promising for environmental applications, but a central challenge is to improve their stability and efficiency for removal processes. Here, the effective incorporation of the MOFs MIL-100(Fe) and ZIF-8 in composite aerogels of chitosan–pectin–lactic acid is reported. The presence of pectin was critical to loading the MOFs efficiently and homogeneously, while the incorporation of lactic acid induced a large increase in the Young’s modulus and provided structural preservation in aqueous solutions. The presence of MOFs enhanced the removal of two dyes, methyl orange (MO) and methylene blue (MB), under batch and flow conditions, with removal efficiencies of methyl orange of about 85% and 90% when loaded with ZIF-8 and MIL-100(Fe), respectively. Bentonite, celite 545, and two ionenes were loaded for comparison. Factors beyond charge-to-charge electrostatic interactions influenced the removal, since no correlations were obtained between the electrical charges of dyes, fillers, and polymers. The kinetic data were analyzed by adapting the Langmuir kinetic model, incorporating absorption and desorption processes, which allowed the recovery of the respective rate constants. Full article

Sourdough fermentation has emerged as a promising biotechnological approach to reducing gluten content and modifying gluten proteins in wheat-based products. This review assesses the current scientific literature on the enzymatic degradation and hydrolysis of gluten during lactic acid bacteria (LAB) sourdough fermentation. It explores implications for individuals with gluten-related disorders, including celiac disease, non-celiac gluten sensitivity and intolerance, as well as irritable bowel syndrome (IBS). In addition, LAB sourdough effect on fermentable oligo-, di-, monosaccharides and polyols (FODMAPs), amylase-trypsin inhibitors (ATIs), and phytate are revised. Selected homo- and heterofermentative LAB are capable of degrading gluten proteins, especially the polypeptides derived from the action of native cereal proteases. Mixed cultures of LAB degrade gluten peptides more effectively than monocultures. However, LAB sourdough is not sufficient to remove the toxic peptides to the minimal level (<20 ppm). This goal is achieved only if sourdough is combined with fungal proteases during sourdough fermentation. LAB sourdough directly contributes to lower FODMAPs but not ATIs and phytate. Phytate is reduced by the endogenous cereal phytases activated at acidic pHs (pH < 5.0), conditions generated during sourdough fermentation. ATIs are also lowered by endogenous cereal proteases instead of LAB proteases/peptidases. Despite LAB sourdough not fully degrading the gluten or directly reducing the ATIs and phytate, it participates through peptidases activity and acidic pH that trigger the action of endogenous cereal proteases and phytases. Full article

Natural organic matter (NOM) in surface waters is a major challenge for drinking water treatment due to its role in the formation of disinfection byproducts (DBPs) during chlorination. This study evaluated the performance of chitosan, a biodegradable coagulant, dissolved in acetic, lactic, and L-ascorbic acids for NOM removal under three turbidity levels (403, 1220, and 5038 NTU). Jar tests were conducted using raw water from the Río Grande de Añasco (Puerto Rico), and TOC, DOC, and UV₂₅₄ were measured at multiple time points. TOC removal ranged from 39.8% to 74.3%, with the highest performance observed in high-turbidity water treated with chitosan–L-ascorbic acid. DOC and UV₂₅₄ reductions followed similar trends, with maximum removals of 76.4% and 76.2%, respectively. Estimated THM formation potential (THMFP) was reduced by up to 81.6%. Across all acids, flocculation efficiencies exceeded 95%. Compared to conventional aluminum-based coagulants, chitosan demonstrated comparable performance, while offering environmental benefits. These results confirm the potential of chitosan–acid systems for effective organic matter removal and DBP control, supporting their application as sustainable alternatives in drinking water treatment. Full article

The by-products that may contain tetrodotoxin (TTX) produced during the processing of farmed pufferfish have caused food safety risks and environmental pollution. Peptidoglycan (PG) of lactic acid bacteria could adsorb TTX; however, its complex structure and poor solubility limited adsorption efficiency. In this study, hydroxyl modifications of three PGs (A3α, A1γ and A4α) were realized via TEMPO-mediated selective oxidation of the primary hydroxyl group. Compared with PGs, it was found that the carboxyl density of hydroxyl-modified PGs (HM-PGs) increased from 1.66 mmol/g to 3.33 mmol/g and the surface electronegativity increased from −36 mV to −59 mV. The adsorption capacity of HM-PGs to TTX reached 1.48 μg/mg, which was comparable to the adsorption of the conventional adsorbent chitosan for aflatoxin B1 (1.39 μg/mg). Moreover, HM-PGs decreased the toxicity of TTX from strong toxic to nearly non-toxic, with the toxicity reduction rate reached 99.85%. After treatment with HM-PGs, the mouse hippocampus and neuronal cell model confirmed that lower neural injury and sodium channel blocking effects were observed in the residual TTX, whose neurotoxicity was lower. Molecular docking simulation and physicochemical analysis revealed that the adsorption of TTX by HM-PGs was a complex adsorption mode driven by the synergy of physicochemical interaction. There were both physical adsorptions based on electrostatic and hydrophobic interactions and chemical binding with strong hydrogen bonding (1.46 Å) and Mayer bond order (0.1229). This study not only developed a new, efficient and safe tool for TTX removal, but also provided a theoretical basis for the development of biological toxin removal material. Full article

Seven wild-type lactic acid bacteria, belonging to Lactiplantibacillus plantarum and Lactococcus cremoris species, were isolated from beetroots and white mushrooms and evaluated for their safety and functional profile. Lc. cremoris isolates were sensitive to all antibiotics tested, while L. plantarum strains exhibited resistance in certain antibiotics. Among them, Lc. cremoris FBMS_5810 showed the highest cholesterol removal ability (51.89%) and adhesion capacity to Caco-2 cell lines (32.14%), while all plant origin strains exhibited strong antagonistic and inhibitory activity against foodborne pathogens, as well as high survival potential during an in vitro digestion model. Subsequently, freeze-dried immobilized Lc. cremoris FBMS_5810 cells on oat flakes were prepared with initial cell loads >8.5 log CFU/g, and the effect of trehalose as a cryoprotectant in cell viability during storage at room and refrigerated temperatures for up to 180 days was studied. A significant reduction in cell loads was observed in all cases studied. However, freeze-dried immobilized Lc. cremoris FBMS_5810 cells on oat flakes prepared using trehalose as a cryoprotectant stored at 4 °C exhibited the highest cell viability (8.75 log CFU/g) after 180 days. In the next step, functional breakfast cereals enriched with freeze-dried immobilized Lc. cremoris FBMS_5810 cells on oat flakes (produced with (MLT) or without (ML) trehalose) were developed and stored at room and refrigerated temperatures for 180 days. The initial cell levels ≥ 9.18 log CFU/g were achieved, while a significant decrease was recorded during storage in all cases. The maintenance of cell loads ≥ 7.75 log CFU/g was documented in the case of both ML and MLT samples stored at 4 °C; however, the presence of trehalose in MLT samples resulted in cell viability 7.52 log CFU/g after 180 days of storage at room temperature. Importantly, the functional breakfast cereals were accepted by the panel during the sensory evaluation. Full article

Background: Pediatric diaphyseal forearm fractures, often caused by sports or leisure activities, require treatment based on fracture stability. While stable fractures can be managed conservatively, unstable fractures typically require surgery, with elastic stable intramedullary (IM) nailing (ESIN) being the gold standard. Bioabsorbable IM nails (BINs) offer an alternative by eliminating the need for implant removal surgery. Methods: Between May 2023 and January 2025, we consecutively managed 161 children with poly-L-lactic-co-glycolic acid (PLGA) BINs in two healthcare centers for diaphyseal forearm fracture and evaluated every reported difficulty and complication of resorbable IM nails. Results: Seven unique peri- or postoperative events occurred during the study period. Even with a high success rate, some complications occurred during the study period. Difficulties and complications were mainly dependent on the surgical technique. Iatrogenic complications such as bone cortex perforation and implant end split were evaluated, along with anatomical variations like focal and general medullary cavity stenosis narrowings that affected implant insertion. Secondary malalignment, one early (2 months) refracture, and one recurrent fracture (2 years) were also noted. Conclusions: Although BINs reduce the need for a second surgery, careful planning, technique, and follow-up are crucial for optimal outcomes. Further research is needed to assess long-term results and complications. Full article

The acid whey derived during the production of Greek yoghurt, i.e., yoghurt acid whey (YAW), is considered as environmental pollutant due to its low pH and high lactose content. YAW may be obtained by centrifugation or ultrafiltration or filtration through cloth bags (traditional method) of the initial yoghurt, methods that could affect its composition. In the last years, efforts have been made to improve its composition using membrane technology. In the present work, the composition of 35 different YAW samples from Greek Yoghurt dairies was studied. The YAW samples were grouped according to the way of production and the kind of milk in the case of the traditional method. The results showed that both the kind of milk and the way of yoghurt staining affected its composition. Ovine YAW derived from traditional straining was richer in lactic acid and calcium than bovine YAW. Moreover, the composition of bovine YAW varied among the different ways of straining, and this affected its behaviour during membrane processing. Nanofiltration of three representative bovine YAW samples and one ovine YAW sample at their natural pH, i.e., pH 4.5, and at 25 °C removed the lactic acid at a range from 40 to 55%, and the monovalent cations > 60% and retained lactose and galactose at percentages > 95% and 80% respectively. Full article

Background: Pediatric olecranon fractures, though rare, can be serious. Treatment varies by type and severity; displaced, intraarticular fractures usually need surgery, where biodegradable implants are emerging as an encouraging option to metal hardware. Methods: Our prospective, single-center, single-arm case series evaluates three pediatric olecranon fracture patients treated by resorbable poly-L-lactic-co-glycolic acid (PLGA) pins and polydioxanone (PDS) loops between Jan 2022 and January 2023 at the Department of Pediatrics, University of Pécs, Clinical Complex. Results: All patients achieved complete radiological healing with excellent functional recovery and no implant-related complications at one-year follow-up. Conclusions: Resorbable PLGA pins and PDS loops provide a promising alternative to conventional metallic fixation in pediatric olecranon fractures, abolishing the need for a second implant-removal surgery while maintaining stability and biocompatibility. Our findings support the child-friendly nature and growing role of biodegradable materials in pediatric fracture management. Full article

The present study aimed to evaluate the antibiofilm effect of combined sonication treatment with organic acids on polystyrene surfaces and to develop a predictive model for the inactivation of Escherichia coli biofilms. Polystyrene plates containing E. coli biofilms were subjected to sonication using different inactivation solutions (PBS, lactic acid, and acetic acid) at varying temperatures (20 °C, 40 °C, and 50 °C) and durations (2 and 5 min). The effects of temperature, treatment duration, and inactivation solution on E. coli biofilm removal were statistically significant (p < 0.05). The use of organic acids, along with increased treatment time and temperature, led to a significant reduction in viable cell counts (0.43–6.21 log CFU/mL) and optical density (0.13–0.72 at OD600) of E. coli biofilms (p < 0.05). The highest E. coli biofilm inactivation, with a reduction of 6.21 CFU/mL and 0.72 OD, was achieved by combining organic acid and thermosonication at 50 °C for 5 min. A significant positive correlation was observed between test methods based on viable cell count and optical density (OD) measurements. According to multiple linear regression analysis results, the R² values of the predictive models for biofilm inactivation, based on viable cell count and OD measurements, were 0.84 and 0.80, respectively. Due to its higher accuracy, the predictive model developed using viable cell count data is recommended for applications in the food industry and processing sectors. Full article

Deep eutectic solvents (DES) represent an innovative and environmentally friendly approach for chitin isolation. Chitin is a natural nitrogenous polysaccharide, characterized by its abundance of amino and hydroxyl groups. The hydrogen bond network in DES can disrupt the crystalline structure of chitin, facilitating its isolation from bioresources by dissolving or degrading other components. DES are known for their low cost, natural chemical constituents, and recyclability. Natural deep eutectic solvents (NADES), a subclass of DES made from natural compounds, offer higher biocompatibility, biodegradability, and the lowest biotoxicity, making them highly promising for the production of eco-friendly chitin products. This review summarized studies on chitin isolation by DES, including reviews of biomass resources, isolation conditions (raw materials, DES compositions, solid–liquid ratios, temperature, and time), and the physicochemical properties of chitin products. Consequently, we have concluded that tailoring an appropriate DES-based process on the specific composition of the raw material can notably improve isolation efficiency. Acidic DES are particularly effective for extracting chitin from materials with high mineral content, such as crustacean bio-waste; for instance, the choline chloride-lactic acid DES achieved purity levels comparable to those of commercial chemical methods. By contrast, alkaline DES are better suited for chitin isolation from protein-rich sources, such as squid pens. DES facilitate calcium carbonate removal through H⁺ ion release and leverage unique hydrogen bonding interactions for efficient deproteination. Among these, potassium carbonate-glycerol DES have demonstrated optimal efficacy. Nonetheless, further comprehensive research is essential to evaluate the environmental impact, economic feasibility, and safety of DES application in chitin production. Full article

Some potentially probiotic strains of lactic acid bacteria (LAB) and yeast that inhabit the digestive tract of humans are known to detoxify xenobiotics, including acrylamide (AA). The objective of the subsequent research was to evaluate the AA-detoxification capability of LAB and yeast isolated from various sources. Namely, the effect of AA was tested on the growth of LAB and yeast strains, as well in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Subsequently, the AA-binding ability of LAB and yeast was investigated in various environments, including the pH, incubation temperature, cell density, and with inanimate cells. The ability of selected LAB and yeast to reduce the genotoxicity of AA was tested on Caco-2 and Hep-G2 cell lines. The results showed that all tested strains exhibited strong resistance to AA at concentrations of 5, 10, and 50 µg/mL. Also, AA was detected in the intracellular and membrane extracts of tested strains. The most effective binding strain was Pediococcus acidilactici 16 at pH = 5, cell density = 10⁹ CFU/mL, and incubation temperature = 37 °C (87.6% of AA removed). Additionally, all tested strains reduced the genotoxicity of AA, with the greatest reduction observed at the highest concentration of 50 µg/mL. The phenomena of detoxification by potentially probiotic strains could reduce the toxic and harmful effects of AA exposure to humans every day. Full article

The need to develop synthetic bone substitutes with structures, properties, and functions similar to bone and capable of preventing microbial infections is still an ongoing challenge. This research is focused on the preparation and characterization of three-dimensional porous scaffolds based on hydroxyapatite (HA)-functionalized calcium carbonate loaded with silver nanoparticles and simvastatin (SIMV). The scaffolds were prepared using the foam replica method, with a polyurethane (PU) sponge as a template, followed by successive polymer removal and sintering. The scaffolds were then coated with poly(lactic-co-glycolic) acid (PLGA) to improve mechanical properties and structural integrity, and loaded with silver nanoparticles and SIMV. The scaffolds were characterized by X-ray powder diffraction (XRD), field emission scanning electron microscopy (FE-SEM), ATR FT-IR, and silver and SIMV loading. Moreover, the samples were analyzed by Brillouin and Raman microscopy. Finally, in vitro bioactivity, SIMV and silver release, and antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis were evaluated. From the Brillouin spectra, samples showed characteristics analogous to those of bone tissue. They exhibited new hydroxyapatite growth, as evidenced by SEM, and good antimicrobial activity against the tested bacteria. In conclusion, the obtained results demonstrate the potential of the scaffolds for application in bone repair. Full article

Cellulose nanofibrils (CNFs) have theoretically ideal properties for bio-based composite applications; however, the incorporation of these materials into polymers is made challenging by the strong binding of water to CNFs and the irreversible agglomeration of CNFs during drying. Previous methods used “contact dewatering”, wherein the addition of wood flour (WF) to CNFs facilitated the mechanical removal of water from the system via cold pressing, which showed potential in producing dried CNF fibrils attached to wood fibers for biocomposite applications. In this work, the viability of contact dewatering with poly(lactic) acid (PLA) powder for PLA/CNF composites was evaluated. The energy efficiency of dewatering, preservation of nanoscale CNF morphology, and mechanical properties were examined by mixing wet CNFs with pulverized PLA at various loading levels, pressing water out of the system, and compression molding and shear mixing composites for testing. The most impressive results from this dewatering method were the preservation of micron-to-nanoscale fibers with high aspect ratios in PLA-CNF composites; increased strength and modulus of 1.7% and 4.2%, respectively, compared to neat PLA; equivalent or better properties than spray-dried nanocellulose at similar loading levels; and an 11-194x reduction in drying energy compared to spray-drying CNFs. Full article

Bacteriocins produced by lactic acid bacteria are known to be useful tools for food biopreservation and fermentation control. Leuconostoc mesenteroides subsp. mesenteroides 406 and 213M0 isolated from different samples of Mongolian traditional fermented milk, airag, had been reported to produce listericidal bacteriocin-like inhibitory substances with similar but slightly different properties. In this study, the antibacterial properties and the related gene sequences of both strains were compared, and then their bacteriocins were purified and identified. Strain 406 was superior to strain 213M0 in cell growth and antibacterial activity against many strains. However, the activity of 213M0 was stronger than that of 406 against a few strains. DNA sequencing revealed two and three plasmids in 406 and 213M0, respectively, and each one of them harbored an almost identical mesentericin Y105–B105 gene cluster. Removal of these plasmids resulted in a complete loss of activity, indicating that the antibacterial activity of both strains was generated by bacteriocins encoded on the plasmids. Mesentericins Y105 and B105 were purified from both cultures, and another novel bacteriocin, named mesentericin M, was identified from the 213M0 culture only. Its structural gene was coded on a 213M0 plasmid and, surprisingly, its C-terminal three amino acid residues were post-translationally cleaved. To our knowledge, this is the first report of a C-terminal truncated bacteriocin. In conclusion, the novel bacteriocin should be mainly responsible for the difference in antibacterial properties between the two strains. Full article

Lignocellulosic biomass is a crucial renewable energy source for producing biofuels and valuable compounds, making it an attractive alternative to fossil resources. In this study, an environmentally friendly method was developed for cellulose fractionation from sugarcane bagasse using deep eutectic solvents (DESs), focusing on achieving high cellulose purity and specific physicochemical properties. The effects of different parameters were investigated by comparing four DESs: choline chloride–lactic acid (ChCl-LA), choline chloride–glycerol (ChCl-G), choline chloride–urea (ChCl-U), and choline chloride–polyalcohol (ChCl-P), under various reaction temperatures and times. The fractionation process was conducted under standard conditions at a temperature of 100 °C for 120 min with a 1:1 molar ratio. The results indicated that all DESs produced comparable cellulose recovery, ranging from 91.83% to 97.07%. A relatively high cellulose recovery was observed in the presence of ChCl-LA, at 95.47%. In addition, ChCl-LA demonstrated the highest efficiency in removing hemicellulose and lignin, at 95.36% and 93.38%, respectively, and high recovery yields of 70.45% for hemicellulose, and 70.66% for the lignin fraction. The fractionation conditions were further optimized using response surface methodology (RSM), achieving a ChCl-LA ratio of 1:2 v/v at 120 °C for 120 min. This resulted in impressive yields: 97.86% cellulose recovery, 96.50% hemicellulose removal, 74.40% hemicellulose recovery, 77.3% lignin recovery, and 71.5% lignin yield from sugarcane bagasse. These results closely match the predicted values, emphasizing the effectiveness of the process and its potential for economic application in lignocellulosic biorefinery operations. Full article