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Keywords = intravirion location

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14 pages, 997 KiB  
Article
Intravirion DNA Can Access the Space Occupied by the Bacteriophage P22 Ejection Proteins
by Justin C. Leavitt, Eddie B. Gilcrease, Brianna M. Woodbury, Carolyn M. Teschke and Sherwood R. Casjens
Viruses 2021, 13(8), 1504; https://doi.org/10.3390/v13081504 - 30 Jul 2021
Cited by 4 | Viewed by 3589
Abstract
Tailed double-stranded DNA bacteriophages inject some proteins with their dsDNA during infection. Phage P22 injects about 12, 12, and 30 molecules of the proteins encoded by genes 7, 16 and 20, respectively. After their ejection from the virion, they assemble into [...] Read more.
Tailed double-stranded DNA bacteriophages inject some proteins with their dsDNA during infection. Phage P22 injects about 12, 12, and 30 molecules of the proteins encoded by genes 7, 16 and 20, respectively. After their ejection from the virion, they assemble into a trans-periplasmic conduit through which the DNA passes to enter the cytoplasm. The location of these proteins in the virion before injection is not well understood, although we recently showed they reside near the portal protein barrel in DNA-filled heads. In this report we show that when these proteins are missing from the virion, a longer than normal DNA molecule is encapsidated by the P22 headful DNA packaging machinery. Thus, the ejection proteins occupy positions within the virion that can be occupied by packaged DNA when they are absent. Full article
(This article belongs to the Special Issue Phage Assembly Pathways - to the Memory of Lindsay Black)
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