Search Results (201)

Bacterial nanocellulose (BNC) is a highly pure biopolymer with promising applications in the biomedical, food, and textile industries. However, the high production costs and low yields obtained in static conditions limit its scalability and industrial applications. This study addresses the sustainable production of BNC using a rotary disk bioreactor (RDB) and explores the use of grape pomace extract as an alternative carbon source for BNC production. Parameters such as the BNC production and biomass yield were evaluated using Komagataeibacter xylinus ATCC 53524 under different operational conditions (disk surface, rotation speed, and number of disks). The results showed that cellulose production increased using silicone-coated disks at 7–9 rpm (up to 2.72 g L⁻¹), while higher yields (5.23 g L⁻¹) were achieved when using grape pomace extract as the culture medium in comparison with conventional HS medium. FTIR and TGA characterizations confirmed that BNC obtained with grape pomace extract presents the same thermal and chemical characteristics than BNC produced with HS medium. This work provides insight into the feasibility of upscaling BNC production using a bioprocessing strategy, combining production in the RDB system and the use of an agro-industrial waste as a sustainable and cost-effective alternative. Full article

Jute, a vital lignocellulosic fiber crop with substantial industrial and ecological relevance, continues to suffer considerable yield and quality degradation due to pervasive foliar pathologies. Traditional diagnostic modalities reliant on manual field inspections are inherently constrained by subjectivity, diagnostic latency, and inadequate scalability across geographically distributed agrarian systems. To transcend these limitations, we propose DERIENet, a robust and scalable classification approach within a deep ensemble learning framework. It is meticulously engineered by integrating three high-performing convolutional neural networks—ResNet50, InceptionV3, and EfficientNetB0—along with regularization, batch normalization, and dropout strategies, to accurately classify jute leaf diseases such as Cercospora Leaf Spot, Golden Mosaic Virus, and healthy leaves. A key methodological contribution is the design of a novel augmentation pipeline, termed Geometric Localized Occlusion and Adaptive Rescaling (GLOAR), which dynamically modulates photometric and geometric distortions based on image entropy and luminance to synthetically upscale a limited dataset (920 images) into a significantly enriched and diverse dataset of 7800 samples, thereby mitigating overfitting and enhancing domain generalizability. Empirical evaluation, utilizing a comprehensive set of performance metrics—accuracy, precision, recall, F1-score, confusion matrices, and ROC curves—demonstrates that DERIENet achieves a state-of-the-art classification accuracy of 99.89%, with macro-averaged and weighted average precision, recall, and F1-score uniformly at 99.89%, and an AUC of 1.0 across all disease categories. The reliability of the model is validated by the confusion matrix, which shows that 899 out of 900 test images were correctly identified and that there was only one misclassification. Comparative evaluations of the various ensemble baselines, such as DenseNet201, MobileNetV2, and VGG16, and individual base learners demonstrate that DERIENet performs noticeably superior to all baseline models. It provides a highly interpretable, deployment-ready, and computationally efficient architecture that is ideal for integrating into edge or mobile platforms to facilitate in situ, real-time disease diagnostics in precision agriculture. Full article

This study investigates how pandemic-induced emotional disruption has reshaped sustainable consumer behavior in the digital age, with a focus on the continued influence of safety measures in the restaurant industry. As societies transition beyond COVID-19 restrictions, health-related anxieties persist, driving consumers to prioritize hygiene and risk reduction in their decision-making. Drawing on large-scale data from TripAdvisor and OpenTable, we analyze the effects of digitally communicated safety protocols on restaurant booking behavior across major U.S. cities. Our findings reveal that safety communication remains a salient factor in consumer choice, even after the acute phase of the pandemic. This effect is particularly pronounced in lower-tier restaurants, where visible digital safety signals help build trust and compensate for weaker brand equity. Conversely, in upscale establishments, where baseline hygiene standards are presumed, the marginal benefit of safety signaling is reduced. The study also identifies enduring patterns of emotional expression and anxiety in online reviews, indicating the long-term psychological imprint of the pandemic on consumer sentiment. By situating safety communication as both a psychological reassurance mechanism and a strategic digital marketing tool, this research contributes to the emerging discourse on sustainable marketing in post-crisis contexts. The results offer theoretical and managerial insights into how businesses can integrate health assurance into long-term brand strategies, reinforcing trust and resilience in digitally mediated, post-pandemic consumption environments. Full article

Anaerobic digestion (AD) offers a sustainable solution by treating biodegradable waste while recovering bioenergy, enhancing the share of renewable energy. Thus, this study aims to investigate the AD for managing and valorizing residues from the potato chip industry: potato peel (PP), potato offcuts (OC), waste cooking oil (WCO), wastewater (WW), and sewage sludge (SS). In particular, the biochemical methane potential (BMP) of each residue, anaerobic co-digestion (AcoD), and greenhouse gas (GHG) emissions of an AD plant are assessed. WW, OC, and SS present a BMP of around 232–280 NmL_CH4/kg of volatile solids (VS). PP and WCO reach a BMP slightly lower than the former substrates (174–202 NmL_CH4/g_VS). AcoD results in methane yields between 150 and 250 NmL_CH4/g_VS. An up-scaled anaerobic digester is designed to manage 1.60 Mg/d of PP. A residence time of 12 days and a digester with 165 m³ is estimated, yielding 14 Nm³_CH4/Mg_VS/d. A simulated AD plant integrated with a combined heat and power unit results in a carbon footprint of 542 kg of CO₂-eq/Mg_db PP, primarily from biogenic GHG emissions. These findings highlight the potential of AD to generate renewable energy from potato industry residues while reducing fossil fuel-related GHG emissions and promoting resource circularity. Full article

As the global plastic pollution crisis intensifies, the development of sustainable food packaging materials has become a priority. Starch-based films present a viable, biodegradable alternative to petroleum-derived plastics but face challenges such as poor moisture resistance and mechanical fragility. This review comprehensively examines state-of-the-art advancements in starch-based packaging, including polymer modifications, bio-nanocomposite incorporation, and innovative processing techniques that enhance functionality. Furthermore, the role of advanced analytical tools in elucidating the structure–performance relationships of starch films is highlighted. In particular, we provide an in-depth exploration of advanced characterization techniques, not only to assess starch-based food packaging but also to monitor starch retrogradation, including Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), nuclear magnetic resonance (NMR), and iodine binding (Blue Value). We also explore cutting-edge developments in active and intelligent packaging, where starch films are functionalized with bioactive compounds for antimicrobial protection and freshness monitoring. While substantial progress has been made, critical challenges remain in upscaling these technologies for industrial production. This review provides a roadmap for future research and the industrial adoption of starch-derived packaging solutions. Full article

Upscaling perovskite solar cells and modules requires precise laser patterning for series interconnection and spatial characterization of cell parameters to understand laser–material interactions and their impact on performance. This study investigates the use of nanosecond (ns) and picosecond (ps) laser pulses at varying fluences for the P3 patterning step of perovskite solar cells. Hyperspectral photoluminescence (PL) imaging was employed to map key parameters such as optical bandgap energy, Urbach energy, and shunt resistance. The mappings were correlated with electrical measurements, revealing that both ns and ps lasers can be utilized for effective series interconnections with minimal performance losses at optimized fluences. Our findings provide a deeper understanding of fluence-dependent effects in P3 patterning. Moreover, the results demonstrate that the process window is robust, allowing for reasonable cell performance even with deviations from optimal parameters. This robustness, coupled with the scalability of the laser patterning process, emphasize its suitability for industrial module production. Full article

Lateral flow tests (LFTs) had a pivotal role in combating the spread of the SARS-CoV-2 virus throughout the COVID-19 pandemic thanks to their affordability and ease of use. Most of LFT devices were based on nitrocellulose membrane strips whose industrial upscaling to billions of devices has already been extensively demonstrated. Nevertheless, the assay option in an LFT format is largely restricted to qualitative detection of the target antigens. In this research, we surveyed the potential of UV nanoimprint lithography (UV-NIL) and extrusion coating (EC) for the high-throughput production of disposable capillary-driven, foil-based tests that allow multistep assays to be implemented for quantitative readout to address the inherent lack of on-demand fluid control and sensitivity of paper-based devices. Both manufacturing technologies operate on the principle of imprinting that enables high-volume, continuous structuring of microfluidic patterns in a roll-to-roll (R2R) production scheme. To demonstrate the feasibility of R2R-fabricated foil chips in a point-of-care biosensing application, we adapted a commercial chemiluminescence multiplex test for COVID-19 antibody detection originally developed for a capillary-driven microfluidic chip manufactured with injection molding (IM). In an effort to build a complete ecosystem for the R2R manufacturing of foil chips, we also recruited additional processes to streamline chip production: R2R biofunctionalization and R2R lamination. Compared to conventional fabrication techniques for microfluidic devices, the R2R techniques highlighted in this work offer unparalleled advantages concerning improved scalability, dexterity of seamless handling, and significant cost reduction. Our preliminary evaluation indicated that the foil chips exhibited comparable performance characteristics to the original IM-fabricated devices. This early success in assay translation highlights the promise of implementing biochemical assays on R2R-manufactured foil chips. Most importantly, it underscores the potential utilization of UV-NIL and EC as an alternative to conventional technologies for the future development in vitro diagnostics (IVD) in response to emerging point-of-care testing demands. Full article

Additive manufacturing (AM) of copper is subject to dynamic development regarding available processes and the quality of produced parts. While challenging, AM processes for copper provide parts with a quality comparable to other metallic material groups like steels. The reasons for the lower prevalence of additive manufacturing of copper components in industrial applications are currently not sufficiently researched, especially in light of the significant progress made in the maturity of this technology. A survey is used to investigate the assessments of protagonists in the field of copper AM. The needs of current and potential users of copper AM are analyzed and outlined. This study reveals that the most relevant technical limitation for users is the reduced surface quality of parts, while overall processes need to become less costly and more reliable to find broader use. Answers given hint to a higher degree of automation, the possibility of multi-material processing, and the upscaling of machine and part sizes as relevant future trends in the copper AM sector. Full article

Incineration remains Europe’s main practice for plastic packaging waste treatment, primarily due to the limitations of mechanical recycling technology. Consequently, research and development of more sustainable and flexible approaches are of high importance. Thermochemical conversion of polypropylene, polystyrene, and municipal plastic packaging mix via high-temperature flash pyrolysis (1000 °C/s) is studied in this research, focusing on the kinetics and yields of the devolatilisation stage. The primary stage results in the formation of volatile organic compounds considered intermediate products for carbon black production. The experiments were conducted in a pressurised wire mesh reactor, investigating the influence of temperature (600–1200 °C), residence time (0.5–10 s), and pressure (1–25 bar). The positive effect of temperature on the volatile yield was observed up to 2–5 s. The devolatilisation stage was completed within a maximum of 5 s at temperatures ranging from 800 to 1200 °C. The pressure was determined to be a kinetically limiting factor of the process to up to 800 °C, and the effect was not present at ≥1000 °C. Raman spectroscopy measurements revealed that pyrolytic carbon deposited on the post-experimental meshes is structurally similar to the industrially produced carbon black. The kinetic data and developed model can be further applied in the upscale reactor design. Full article

The shift from fossil-based packaging materials to more sustainable alternatives is driven by evolving environmental regulations aiming for enhanced recyclability and biodegradability. Dispersion coatings, as opposed to extrusion-based approaches, offer significant advantages by reducing the coat weights, but generally, multiple coating layers are needed to meet functional performance requirements. This study explores the application of upscaled multilayer dispersion coatings comprising polylactic acid-based coating (PLAX) and hybrid nanomaterial lacquer (bioORMOCER^®) on commercial base papers for barrier packaging using semi-pilot reverse gravure and industrial-scale rod coaters. One multilayer structure demonstrated a low water vapour transmission rate (WVTR), achieving a WVTR of 12 g/(m²·day) under standard conditions and a 78% reduction of WVTR compared to the substrate under elevated humidity. The other multilayer structure exhibited an excellent oxygen transmission rate (OTR) of 2.3 cc/(m²·day·bar) at dry conditions, which is comparable to conventional high-performance alternatives. Both multilayer coatings enhanced the grease and mineral oil barriers significantly, as heptane vapour transmission rate (HVTR) reductions exceeded 97%. The multilayer coatings demonstrated strong potential for scalable production of sustainable, high-barrier packaging materials. These findings highlight the capability of dispersion coatings to replace traditional fossil-based barriers, advancing the development of environmentally friendly packaging solutions. Full article

Dendrobine, a significant medicinal compound, typically accumulates at low concentrations within several Dendrobium species, including Dendrobium nobile, Dendrobium catenatum, and Dendrobium moniliforme. As D. nobile and D. catenatum are established ingredients in traditional Chinese medicine and have been cultivated extensively, they present ideal plant chassis for upscaling dendrobine production for industrial and research applications. This study employed two approaches: the ectopic overexpression of seven genes through multigene stacking and the activation of multiple key endogenous genes in the dendrobine synthesis pathway using CRISPR/Act3.0 in either D. nobile or D. catenatum. These methods enhanced dendrobine production in transiently infiltrated leaves by 30.1% and transgenic plants by 35.6%. The study is the first to apply CRISPR/Act3.0 to Dendrobium orchids, enhancing dendrobine production, and thus laying a solid foundation for further improvements. CRISPR/Act3.0 is a recently developed technique that demonstrates high efficiency in model plant species, including rice, maize, and Arabidopsis. By combining CRISPR with transcriptional regulatory modules, activation of multiple endogenous genes in the metabolic pathway can be achieved. The successful application in orchid molecular breeding reveals promising potential for further exploration. Full article

Hydrothermal liquefaction (HTL) technology has garnered immense research interest due to its potential to convert wet biomass into petroleum-like biocrude. Understanding the reaction mechanism and kinetics of HTL is crucial for understanding the process better, estimating the yields, and scaling up. On the other hand, reaction mechanisms and kinetics largely depend upon the feedstock composition and reaction parameters of HTL. However, the literature lacks an in-depth analysis of the reaction mechanism and kinetics concerning biocrude yield and product distribution for a single to multi-feedstock scenario. This review focuses on the reaction mechanisms of various biomolecular components of lignocellulosic biomass, proteins, and lipids in the HTL process under sub- and supercritical conditions. Furthermore, the HTL reaction kinetics, effect of reaction conditions on reaction mechanisms, and product distribution are explored. The findings agree that reaction temperature and retention time follow inverse relations for high biocrude yield. A high heating rate is recommended for higher biocrude yield to avoid cracking and recombination processes. A high solvent/feedstock ratio, depending on feedstock composition, was favored for optimum biocrude yield. In addition, catalysts and reaction solvents, especially organic solvents, effectively contribute towards high biocrude yield, even up to 70%. Heterogeneous catalysts are favored due to reusability and improved biocrude quality. Also, hydrothermal co-liquefaction (multi-feedstock) use for improving biocrude yield was debated. A detailed discussion on the reaction kinetics of various biomolecular components in the HTL process revealed that reactions in HTL normally follow the first-order rate law. Finally, the authors outline the pointers for future research in HTL for industrial upscaling. Full article

The hydrogen plasma smelting reduction process has the potential to drastically reduce the CO₂ emissions of the steel industry by using molecular, atomic and ionized hydrogen as a reducing agent for iron ores. To increase the hydrogen and thermal efficiency of the process, a pre-reduction and pre-heating stage should be incorporated in a future upscaling of an existing HPSR demonstration plant within the scope of the “SuSteel follow-up” project to a target capacity of 200 kg/h of iron ore. The determination of the optimal process parameters is followed by a review of possible reactor types. A fluidized bed cascade, a cyclone cascade and a rotary kiln are compared for this purpose. Their applicability for the hydrogen plasma smelting is discussed, based on their fundamental design and operational procedures. Additionally, critical features of the different reactor types are outlined. A cyclone cascade with at least 3 stages is proposed to be the optimal reactor for pre-heating and pre-reducing the input material for the upscaled hydrogen plasma smelting reduction demonstration plant, based on the assessment. Full article

In this study, we assess an alternative Fc-fusion protein purification method that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc domain of human IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed of the bathophenanthroline (henceforth, batho) chelator with either Zn²⁺ or Cu²⁺ ions (i.e., [(batho)₃:Zn²⁺] or [(batho)₂:Cu²⁺]) in the presence of polyethylene glycol 6000 (PEG-6000). In a three-step purification process conducted at pH 7, AChE-Fc was captured by the aromatic complexes (Step 1); unbound or weakly bound protein impurities were removed with 20 mM NaCl (Step 2); and AChE-Fc was then extracted at pH 7 (Step 3) using 100 mM Na citrate buffer in 250 mM NaCl. Purified AChE-Fc was not aggregated (as determined by dynamic light scattering (DLS) and Native PAGE). However, full enzymatic activity was only preserved with the [(batho)₃:Zn²⁺] complex. Interaction between AChE-Fc and [(batho)₃:Zn²⁺] led to ~83–88% overall protein yield. Thirty-fold process upscaling by volume required only proportional increase in the amounts of [(batho)₃:Zn²⁺] and PEG-6000. Efficient (95–97%) chelator recycling was achieved by recrystallization. Chelator leaching into purified AchE-Fc was estimated to be ~0.3% relative to the total amount used. Taken together, this novel procedure has the potential to provide an economical and practical avenue for the industrial purification of Fc-fusion proteins. Full article

While multilayer plastic is difficult for recycling, innovative technologies and tactics are being developed to improve the process. New technologies in chemical recycling show promising results; however, the net improvement brought to the environment, economy, and society should be assessed for their wider adoption and diffusion. This study focuses on a Life Cycle Sustainability Assessment (LCSA) of an innovative process for the sorting and chemical recycling of multilayer plastic packaging waste from post-consumer sources to obtain new packaging for the food industry. The analysis indicated that the packaging made of rPET obtained through depolymerization is environmentally and economically competitive compared to the virgin PET. Packaging made of rPET and rLDPE obtained through delamination usually performed worse (or comparable) than the virgin counterparts. The social impact assessment indicated some areas of concern (e.g., workers’ health and safety risks due to exposure to hazardous substances), as well as potential opportunities (e.g., improved local employment). This paper is the first to present a synergetic approach to the sustainability assessment of chemical recycling technologies to obtain new high-performance packaging solutions. It provides useful insights to academics, managers, and decision makers in the plastic recycling sector whether (and under what conditions) the chemical recycling of multilayer plastic waste is feasible from the environmental, economic, and social perspectives. Despite associated uncertainties, the results are promising as an attractive option for further research, optimization, and upscaling. Full article