Search Results (6,463)

Understanding the migratory strategies of plateau-endemic species is essential for informing effective conservation, especially under climate change. The Black-necked Crane (Grus nigricollis), a high-altitude specialist, has shown notable population growth in recent years. We analysed satellite tracking data from 16 individuals of a western subpopulation in the lake basin region of northern Tibet (2021–2024), focusing on migration patterns, stopover use, and habitat selection. This subpopulation exhibited short-distance (mean: 284.21 km), intra-Tibet migrations with low reliance on stopover sites. Autumn migration was shorter, more direct, higher in altitude, and slower in speed than spring migration. Juveniles used smaller, more fragmented habitats than subadults, and their spatial range expanded over time. Given these patterns, we infer that the short-distance migration strategy may reduce energetic demands and mortality risks while increasing route flexibility—characteristics that may benefit population growth. We refer to this as a low-energy, high-efficiency migration strategy, which we hypothesise could support faster population growth and enhance resilience to environmental change. We recommend prioritizing the conservation of short-distance migration corridors, such as the typical lake basin area in northern Tibet–Yarlung Tsangpo River system, which may help sustain plateau-endemic migratory populations under future climate scenarios. Full article

This study examines the influence of mass media on the career choices of high school students from Brașov County, Romania, with a focus on their underlying motivational factors. Employing a quantitative design, it draws on data from a standardized questionnaire completed by 1314 students from local high schools. Descriptive and inferential statistical methods were used in the analysis. While some students identify mass media as a key source of career guidance, documentaries and career fairs are more frequently cited as trusted sources. Students’ perceptions of mass media are ambivalent: 55.1% see it as manipulative, while 41.7% and 24.7% acknowledge its informative and educational roles. Personal motivation emerges as the most significant influence, with 64.8% guided by individual talents and abilities, compared to a lower influence from family or media role models. Correlational analysis highlights the importance of personal development, creativity, and collaboration in career motivation. This study suggests that mass media indirectly shapes students’ aspirations by reinforcing values like social recognition, mobility, and identity. Finally, it reveals a strong link between career interest and expectations for respectful, stable, and growth-oriented work environments, pointing to a pragmatic orientation toward professional sustainability. Full article

Common prosperity serves as a pivotal condition for achieving sustainable development by fostering social equity, bolstering economic resilience, and promoting environmental stewardship. Differential land revenue, as a crucial form of property based on spatial resource occupation, significantly contributes to the achievement of common prosperity, though empirical evidence of its impact is limited. This study explores the potential influence of land utilization revenue disparity on common prosperity from the perspective of urban macro differential rent (UMDR). Utilizing panel data from 280 Chinese cities spanning 2007 to 2020, we discover that UMDR and common prosperity levels exhibit strikingly similar spatiotemporal evolution. Further empirical analysis shows that UMDR significantly raises urban common prosperity levels, with a 0.217 standard unit increase in common prosperity for every 1 standard unit rise in UMDR. This boost stems from enhanced urban prosperity and the sharing of development achievements, encompassing economic growth, improved public services, enhanced ecological civilization, and more equitable distribution of development gains between urban and rural areas and among individuals. Additionally, we observe that UMDR has a more pronounced effect on common prosperity in eastern cities and those with a predominant service industry. This study enhances the comprehension of the relationship between urban land revenue disparities, prosperity, and equitable sharing, presenting a new perspective for the administration to contemplate the utilization of land-based policy tools in pursuit of the common prosperity goal and ultimately achieve sustainable development. Full article

The aim was to investigate the genetic effects of a SNP located in the precursor region of gga-miR-3528. (1) Single-nucleotide polymorphisms within precursor regions of microRNAs play crucial biological roles. (2) Utilizing a Gushi–Anka F₂ resource population (n = 860), we screened and validated miRNA SNPs. A SNP mutation in the miR-3528 precursor region was identified. Specific primers were designed to amplify the polymorphic fragment. Genotyping was performed for this individual SNP across the population, using the MassArray system. Association analyses were conducted between this SNP and chicken growth and body measurement traits, carcass traits, meat quality traits, and serum enzyme activities. (3) The rs14098602 (+12 bp A > G) was identified within the precursor region of gga-miR-3528. Significant associations (p < 0.05) were observed between this SNP and chicken growth traits (body weight at the age of 0 day, body weight at the age of 2 weeks, and body weight at the age of 4 weeks), carcass traits (evisceration weight), meat quality traits (subcutaneous fat rate and pectoral muscle density), and serum enzyme activities (total protein, albumin, globulin, cholinesterase, and lactate dehydrogenase). (4) These findings suggest that the polymorphism at rs14098602 may influence chicken growth, meat quality, and serum biochemical indices, through specific mechanisms. The gga-miR-3528 gene likely plays an important role in chicken development. Therefore, this SNP can serve as a molecular marker for genetic breeding and auxiliary selection of growth-related traits, facilitating the rapid establishment of elite chicken populations with superior genetic resources. Full article

Precise recognition of individual ovine specimens plays a pivotal role in implementing smart agricultural platforms and optimizing herd management systems. With the development of deep learning technology, sheep face recognition provides an efficient and contactless solution for individual sheep identification. However, with the growth of sheep, their facial features keep changing, which poses challenges for existing sheep face recognition models to maintain accuracy across the dynamic changes in facial features over time, making it difficult to meet practical needs. To address this limitation, we propose the lifelong biometric learning of the sheep face network (LBL-SheepNet), a feature decoupling network designed for continuous adaptation to ovine facial changes, and constructed a dataset of 31,200 images from 55 sheep tracked monthly from 1 to 12 months of age. The LBL-SheepNet model addresses dynamic variations in facial features during sheep growth through a multi-module architectural framework. Firstly, a Squeeze-and-Excitation (SE) module enhances discriminative feature representation through adaptive channel-wise recalibration. Then, a nonlinear feature decoupling module employs a hybrid channel-batch attention mechanism to separate age-related features from identity-specific characteristics. Finally, a correlation analysis module utilizes adversarial learning to suppress age-biased feature interference, ensuring focus on age-invariant identifiers. Experimental results demonstrate that LBL-SheepNet achieves 95.5% identification accuracy and 95.3% average precision on the sheep face dataset. This study introduces a lifelong biometric learning (LBL) mechanism to mitigate recognition accuracy degradation caused by dynamic facial feature variations in growing sheep. By designing a feature decoupling network integrated with adversarial age-invariant learning, the proposed method addresses the performance limitations of existing models in long-term individual identification. Full article

Degenerative disc disease (DDD) is a major cause of chronic low back pain (LBP), yet the molecular mechanisms driving disc degeneration and pain remain poorly understood. This study analyzed intervertebral disc (IVD) tissue from 36 young patients (median age = 36.00 [31.00, 42.50] years) with herniated discs and LBP, alongside healthy controls, to investigate changes in the extracellular matrix (ECM) and neurochemical alterations. Disc degeneration was assessed using MRI (Pfirrmann grading) and histology (Sive’s criteria). Histochemical and immunohistochemical methods were used to evaluate aggrecan content, calcification, and the expression of nerve growth factor (NGF), substance P (SP), and S-100 protein. MRI findings included Pfirrmann grades V (30.55%), IV (61.11%), III (5.56%), and II (2.78%). Severe histological degeneration (10–12 points) was observed in three patients. Aggrecan depletion correlated with longer pain duration (r = 0.449, p = 0.031). NGF expression was significantly elevated in degenerated discs (p = 0.0287) and strongly correlated with SP (r = 0.785, p = 5.268 × 10⁻⁹). Free nerve endings were identified in 5 cases. ECM calcification, present in 36.1% of patients, was significantly associated with radiculopathy (r = 0.664, p = 0.005). The observed co-localization of NGF and SP suggests a synergistic role in pain development. These results indicate that in young individuals, aggrecan loss, neurochemical imbalance, and ECM calcification are key contributors to DDD and chronic LBP. Full article

Due to small population size, Population Viability Analysis (PVA) of endangered species often pools all individuals into a single matrix to decrease variation in estimation of transition rates. These pooled populations may mask significant environmental variation among populations, affecting estimates. Using 10 years of population data (2000–2010) on the endangered plant Jacquemontia reclinata in Southeastern Florida, USA, I parameterized a stage-structured matrix model and calculated annual growth rates (lambdas)and elasticity for each year using stochastic matrix models. The metapopulation model incorporating actual dynamics of the two largest populations showed a lower occupancy rate and higher risk of extinction at an earlier time compared to a model that used the average of all natural populations. Analyses were consistent that incorporating population variation versus average dynamics in modeling J. reclinata demography results in more variation and greater extinction risk. Local variation may be due to both weather (including minimum winter temperature and total annual precipitation) and local disturbance dynamics in these urban preserves. Full article

Background/Objectives: Lateral patellar dislocation (LPD) is a common pathology of the adolescent knee and a major predisposing factor for patellofemoral instability (PFI). The pathogenesis of PFI involves a combination of anatomical and biomechanical contributors, with increasing evidence pointing to sex-specific differences in knee morphology. Despite this, the developmental course of these parameters and their variation between sexes remain insufficiently characterized. This study aims to investigate sex-related differences in patellofemoral joint geometry among skeletally immature patients with a history of PFI, focusing on how these anatomical variations evolve with increasing knee size, as represented by femoral condylar width. Methods: A total of 315 knee MRIs from patients under 18 years with documented PFI were retrospectively analyzed. Trochlear morphology, patellar tilt, axial positioning, and sagittal alignment were assessed using established MRI-based parameters. All measurements were normalized to bicondylar width to account for individual knee size. Sex-specific comparisons were performed using independent t-tests and linear regression analysis. Results: Females exhibited significantly smaller femoral widths, shallower trochlear depth (TD), shorter tibial tubercle–posterior cruciate ligament (TTPCL) distances, and lower patellar trochlear index (PTI) values compared to males (p < 0.05). In males, increasing femoral width was associated with progressive normalization of patellar tilt and sagittal alignment parameters. In contrast, these alignment parameters in females remained largely unchanged or worsened across different femoral sizes. Additionally, patellar inclination angle and PTI were significantly influenced by knee size in males (p < 0.05), whereas no such relationship was identified in females. Conclusions: Sex-specific morphological differences in patellofemoral geometry are evident early in development and evolve distinctly with growth. These differences may contribute to the higher prevalence of PFI in females and underscore the importance of considering sex and knee size in anatomical assessments. Full article

Nano- and microplastic pollution, together with the ongoing rise in global temperatures driven by climate change, represent increasingly critical environmental challenges. Although these stressors often co-occur in the environment, their combined effects on plant systems remain largely unexplored. To test the hypothesis that their interaction may exacerbate the effects observed under each stressor individually, we investigated the response of seedlings of Triticum turgidum to treatments with fluorescent polystyrene nanoplastics under optimal (25 °C) and elevated (35 °C) temperature conditions. We evaluated seedling growth, photosynthetic pigment content, and oxidative stress markers using both biochemical and histochemical techniques. In addition, we assessed enzymatic and non-enzymatic antioxidant responses. The use of fluorescently labeled nanoplastics enabled the visualization of their uptake and translocation within plant tissues. Elevated temperatures negatively affect plant growth, increasing the production of proline, a key protective molecule, and weakly activating secondary defense mechanisms. Nanoplastics disturbed wheat seedling physiology, with these effects being amplified under high temperature conditions. Combined stress enhances nanoplastic uptake in roots, increases oxidative damage, and alters antioxidant responses, reducing defense capacity in leaves while triggering compensatory mechanisms in roots. These findings underscore a concerning interaction between plastic pollution and climate warming in crop plants. Full article

The use of eco-organic ingredients as a source of protein in aquaculture diets needs important attention due to the growing demand for organic seafood products. The present study evaluated the effects of fish meal substitution by different organic ingredients on the growth, body composition, retention efficiency, enzyme activity, and nutrient digestibility of white shrimp Penaeus vannamei. The four dietary formulations tested were formulated with organic ingredients and the fish meal was replaced by the following organic protein meals: Iberian pig viscera meal (PIG), trout by-product meal (TRO), insect meal (FLY), and organic vegetable meal (WHT), in addition to a control diet (CON) that included 15% fish meal. A growth trial was carried out for 83 days, raising 1 g shrimp to commercial size (20 g). Shrimp were stocked at 167 shrimp/m³ (15 individuals per 90 L tank). The results showed that the growth obtained by shrimp fed with TRO (19.27 g) and PIG (19.35 g) were similar in weight gain to the control diet (20.76 g), while FLY (16.04 g) and WHT (16.73 g) meals resulted in a significant lower final weight. The FLY diet showed significantly lower protein digestibility (68.89%) compared to the CON, PIG, TRO, and WHT diets, and significantly higher trypsin activity (0.17 mU/g) compared to shrimp fed with the PIG, TRO, and WHT diets. Shrimp fed with WHT have a significantly lower body weight percentage of protein (19.69%) than shrimp fed with the WHT and TRO diets, and some significant differences in dietary aminoacidic levels affecting amino acid body composition. These results indicate that Iberian pig viscera and trout by-product meal can successfully replace fish meal in Pacific white shrimp aquaculture. Full article

Background: Central precocious puberty (CPP), defined as the onset of secondary sexual characteristics before age 8 in girls, is increasingly prevalent worldwide. CPP is often caused by early activation of the HPG axis, leading to accelerated growth and bone maturation. However, the diagnostic accuracy of standard bone age (BA) methods remains uncertain in this context. Objective: To compare the diagnostic accuracy of the Greulich–Pyle atlas (GPA) and Tanner–Whitehouse 3 (TW3) methods in estimating skeletal age in girls with CPP and to assess the predictive value of serum hormone levels for estimating chronological age (CA). Methods: An observational, cross-sectional diagnostic study was conducted, involving n = 109 girls aged 6–12 years with confirmed CPP (Ethics Committee approval: CHUC_2023_86; 13 July 2023). Left posteroanterior hand–wrist (PA–HW) radiographs were assessed using the GPA and TW3 methods. Anthropometric measurements were recorded, and serum concentrations of estradiol, LH, FSH, DHEA-S, cortisol, TSH, and free T4 were obtained. Comparisons between CA and BA estimates were conducted using repeated-measures ANOVA, and ANCOVA was applied to examine the hormonal predictors of CA. Results: Both GPA and TW3 overestimated CA between 7 and 12 years, with the GPA showing larger deviations (up to 4.8 months). The TW3 method provided more accurate estimations, particularly at advanced pubertal stages. Estradiol (η²_p = 0.188–0.197), LH (η²_p = 0.061–0.068), and FSH (η²_p = 0.008–0.023) emerged as the strongest endocrine predictors of CA, significantly enhancing the explanatory power of both radiological methods. Conclusions: The TW3 method demonstrated superior diagnostic accuracy over GPA in girls with CPP, especially between 7 and 12 years. Integrating estradiol, LH, and FSH into BA assessment significantly improved the accuracy, supporting a more individualized and physiologically grounded diagnostic approach. Full article

Background/Objectives: Prior research suggest that poor working memory significantly contributes to the growth of overweight and obesity. This study investigated the behavioral and neural aspects of general and food-specific working memory in females with overweight or obesity (OW/OB). Method: A total of 54 female participants, with 26 in the OW/OB group and 28 in the normal-weight (NW) group, completed a general and a food-related two-back task while an EEG was recorded. Results: In the general task, the OW/OB group showed significantly poorer performance (higher IES) than the NW group (p = 0.018, η² = 0.10), with reduced theta power during non-target trials (p = 0.040, η² = 0.08). No group differences were found for P2, N2, or P3 amplitudes. In the food-related task, significant group × stimulus interactions were observed. The OW/OB group showed significantly higher P2 amplitudes in high-calorie (HC) versus low-calorie (LC) food conditions (p = 0.005, η² = 0.15). LPC amplitudes were greater in the OW/OB group for HC targets (p = 0.036, η² = 0.09). Alpha power was significantly lower in OW/OB compared to NW in HC non-targets (p = 0.030, η² = 0.09), suggesting a greater cognitive effort. Conclusions: These findings indicate that individuals with OW/OB exhibit deficits in general working memory and heightened neural responses to high-calorie food cues, particularly during non-target inhibition. The results suggest an interaction between reward salience and cognitive control mechanisms in obesity. Full article

This literature-based review draws on studies of thirty-four fish species; most are from northern temperate regions. Fish have flexible and indeterminate growth, and often they do not reach their growth and size potential. They may become stunted with impaired growth and early maturity, chiefly as a phenotypically plastic reaction. The main causes of stunted growth are negatively density-dependent food availability and keen intraspecific competition leading to environmental stress. Typically, their growth levels off early in life as energy consumptions approach energy costs of maintenance. Females typically attain maturity soon after the energy surplus from feeding starts to decrease. Males are often more variable in size at maturity owing to alternative mating strategies, and their size at maturity depends on both species-specific mating behaviours and environmental opportunities. In polyphenic/polymorphic populations, one phenotype may be stunted and the other phenotype non-stunted; stunted individuals do not perform the required ontogenetic niche shift needed to grow larger. The adult morphology of stunted fish is typically like the morphology of juveniles. Their secondary sexual characters are less pronounced, and they phenotypically retain adaptation to their early feeding niche, which is different from that of large-growing individuals. There are open questions regarding to what extent genetics and epigenetics regulate the life histories of stunted phenotypes. Full article

In this study, an in vitro co-culture system of Castilleja tenuiflora and its host, Baccharis conferta, was used, and the impact of their interaction on specialized metabolite content was analyzed. After 4 weeks of co-culture, haustoria formation was verified through environmental scanning electron and confocal microscopy, confirming the successful establishment of the plant–plant interaction. Shoot height and biomass of the aerial part of the hemiparasite were not affected significantly by co-culture. However, root biomass increased by 53% compared to individually grown plants. Co-culture significantly reduced the host’s root length without negatively affecting its overall growth or survival. Phytochemical profile alterations were observed in both species. For C. tenuiflora, the lignans sesamin and eudesmin are proposed as differentially accumulated metabolites, while in B. conferta, the caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and the flavonoid acacetin were expressed differently. The development and chemical profiles of B. conferta and C. tenuiflora change when they grow in a co-culture because of the host–parasite interaction. Here, we report the feasibility of using a hemiparasite–host system to investigate more profound research questions. Future biotechnological applications of this system include elucidating the genetic regulators involved in haustorium formation, as well as optimizing environmental and physiological conditions to enhance its biosynthetic capacity for the production of specialized metabolites with therapeutic value. Full article

Cultured meat is emerging as a sustainable alternative to conventional animal agriculture, with scaffolds playing a central role in supporting cellular attachment, growth, and tissue maturation. This review focuses on the development of gel-based hybrid biomaterials that meet the dual requirements of biocompatibility and food safety. We explore recent advances in the use of naturally derived gel-forming polymers such as gelatin, chitosan, cellulose, alginate, and plant-based proteins as the structural backbone for edible scaffolds. Particular attention is given to the integration of food-grade functional additives into hydrogel-based scaffolds. These include nanocellulose, dietary fibers, modified starches, polyphenols, and enzymatic crosslinkers such as transglutaminase, which enhance mechanical stability, rheological properties, and cell-guidance capabilities. Rather than focusing on fabrication methods or individual case studies, this review emphasizes the material-centric design strategies for building scalable, printable, and digestible gel scaffolds suitable for cultured meat production. By systemically evaluating the role of each component in structural reinforcement and biological interaction, this work provides a comprehensive frame work for designing next-generation edible scaffold systems. Nonetheless, the field continues to face challenges, including structural optimization, regulatory validation, and scale-up, which are critical for future implementation. Ultimately, hybrid gel-based scaffolds are positioned as a foundational technology for advancing the functionality, manufacturability, and consumer readiness of cultured meat products, distinguishing this work from previous reviews. Unlike previous reviews that have focused primarily on fabrication techniques or tissue engineering applications, this review provides a uniquely food-centric perspective by systematically evaluating the compositional design of hybrid hydrogel-based scaffolds with edibility, scalability, and consumer acceptance in mind. Through a comparative analysis of food-safe additives and naturally derived biopolymers, this review establishes a framework that bridges biomaterials science and food engineering to advance the practical realization of cultured meat products. Full article