Search Results (117)

Fat-tailed dunnarts (Sminthopsis crassicaudata) are an emerging model species for developmental, reproductive, and conservation biology research. Understanding their husbandry and enrichment needs is integral to ensuring best welfare in captive-bred populations. This was made evident following the sudden deaths of three dunnarts from a population housed at a university research facility between 2018 and 2022. Necropsy results revealed significant trichobezoars causing gastrointestinal obstruction. Following these findings, adjustments were made to the dunnart enclosures with the addition of autoclaved tree bark to capture loose fur as well as the inclusion of paraffin oil in the diet for the purpose of gastrointestinal lubrication. Since these husbandry interventions, no further deaths attributed to trichobezoars have been reported. Here, we present these data and outline new best practice methods for captive dunnart husbandry. Full article

The welfare of animals in zoos has come under increasing scrutiny as public awareness grows around the biological needs of captive species. It is also becoming clear that promoting positive welfare experiences upholds population management and conservation aims. This paper re-evaluates current welfare frameworks in zoological institutions, advocating for evidence-based practices, multi-dimensional welfare metrics, and greater emphasis on species-specific needs, as well as the importance of input-based approaches to assess the welfare of zoo animals. By evaluating the limitations of current welfare practices (e.g., a lack of species-specific assessment protocols or sound husbandry evidence to base measures on) and presenting potential areas for improvement, this paper identifies ways that sound baselines for meaningful zoo animal welfare outputs can be created. Although current welfare policies from large zoo membership organisations stipulate assessment of welfare outputs as key to improving animal welfare standards, such outputs can only be positive if inputs are species-specific and relevant to the animals being housed. Practices such as the use of environmental enrichment (for example) need to be further refined to ensure they provide meaningful outputs (for the individuals) from the inputs that create them. Understanding the animal’s needs to ensure that the goal of enrichment is clear benefits both the animal who is provided with the enrichment and the human caregivers as husbandry and management becomes easier. A focus on welfare outputs is commendable and (especially when considering emotional outputs) is indeed a gold standard to aim for, yet we must not lose sight of striving for improvements to housing, husbandry, and species-specific care. Without such fundamental support from correct inputs, outputs are unlikely to be truly (or meaningfully) positive. Therefore, consistent re-examination of inputs is required to make sure they uphold an individual’s attainment of good welfare. Full article

Feather pecking (FP) is a significant welfare and economic problem in laying hen husbandry. While there is growing evidence that the glutamatergic system plays a crucial role in regulating FP behavior, the biological mechanisms remain unclear, largely due to the limited uptake of peripheral glutamate across the blood–brain barrier (BBB). Here, we applied a multi-omics approach combined with physiology assays to answer this question from the perspective of the gut–brain axis. A total of 108 hens were randomly assigned to two groups (treatment and control) with six replicates each, and the treatment group was subjected to chronic environmental stressors including re-housing, noise, and transport. We found that chronic exposure to environmental stressors induced severe FP, accompanied by reduced production performance and increased anxiety- and depression-related behaviors, compared to controls. In addition, the immune system was potentially disrupted in FP chickens. Notably, gut microbiota diversity and composition were significantly altered, leading to decreased microbial community stability. Non-targeted metabolomic analysis identified a variety of differential metabolites, primarily associated with arginine and histidine biosynthesis. A significant increase in glutamate levels was also observed in the hippocampus of FP chickens. Transcriptome analysis revealed the upregulated expressions of glutamate-related receptors GRIN2A and SLC17A6 in the hippocampus. Correlation analysis indicated that GRIN2A and SLC17A6 are positively associated with arginine levels in the duodenum, while Romboutsia in the duodenum is negatively correlated with arginine. These findings suggest that intestinal bacteria, including Romboutsia, may influence FP behavior by altering plasma arginine and histidine levels. These changes, in turn, affect glutamate levels and receptor gene expression in the hippocampus, thereby regulating the glutamatergic system. Our research offers insights into novel strategies for mitigating harmful behaviors in poultry farming, with potential benefits for animal performance and welfare. Full article

Research on the welfare of pet guinea pigs and connections between their behaviour and husbandry practices is scarce. Through an online survey among 1181 German-speaking guinea pig caretakers, we investigated husbandry practices (including social composition, housing type, free roaming, enrichment, and feeding), human–animal interactions, and guinea pig behaviour. Most participating caretakers seemed attentive to their animals’ needs and welfare, as shown by the prevalence of self-built enclosures (42.8%) and fenced floor areas (21.1%), with various enrichment and furnishings, and mostly balanced nutrition. Husbandry practices that can negatively impact welfare, such as individual housing and no constant access to hay, were observed for 7.5% and 0.4% of the animals, respectively. Eating and resting next to conspecifics and locomotor play occurred several times per day in 87.6%, 48.1%, and 19.9% of animals, respectively. Biting, running up and down and bar chewing were rare (several times per day in 0.4%, 2.9%, and 0.7% of animals, respectively). We found significant associations of guinea pig behaviour with human–animal interactions and housing conditions. For instance, affiliative behaviour and locomotor play and use of enrichment were more frequently reported in the case of more frequent positive human–animal interactions and food enrichment. Our results provide additional knowledge to support consultations on behaviour and husbandry practices. Full article

Non-human primates (hereafter ‘primates’) constitute a common group of animals in captivity but their captive maintenance involves ethical and husbandry issues, many of which can be addressed through environmental enrichment (hereafter ‘enrichment’). Enrichment is often applied in a trial-and-error fashion or based on subjective assessments of efficacy. Thus, a predictive framework for enrichment is necessary to ensure it achieves the desired outcomes. As one of the initial steps towards the development of a predictive enrichment science, we aimed to identify the most effective enrichment approaches for captive primates. We conducted a comprehensive meta-analysis of the peer-reviewed literature and extracted information on methodological approaches, experimental design, species and environmental context. We developed and applied a novel efficacy index score to each study protocol as a comparable metric of enrichment efficacy in order to appraise current enrichment practices. Our findings suggest that captive primate enrichment approaches vary in their efficacy, with training-based enrichment being the most effective. Furthermore, the social context of subjects appears to influence efficacy, with primates housed alone deriving the most benefit from enrichment. A species’ natural minimum group size may also influence enrichment efficacy but this relationship requires further investigation. Testing and reporting trends for captive primate enrichment are presented and discussed, highlighting important gaps in the literature and avenues of future research while identifying the beneficial effects of different enrichment practices. Full article

One of the primary challenges encountered in managing captive callitrichids is a group of gastrointestinal disorders that are collectively known as Marmoset Wasting Syndrome (MWS). Unfortunately, MWS is still not well understood. It is suggested that MWS has a multifactorial etiology; stress, infection, malnutrition, malabsorption, and enteritis or colitis may be associated with MWS. Usually, animals demonstrate more than one of the following clinical signs: progressive weight loss, alopecia, chronic diarrhea, muscle atrophy, hypoproteinemia, and anemia. Recent advances in diagnostics, mostly noninvasive fecal and urine tests, yielded promising results. Good treatment results have been achieved with the administration of glucocorticoids and tranexamic acid, although no cure has been found yet. Probiotics and gel diets also demonstrated to be potentially useful. However, once animals are affected by MWS, treatments eventually become ineffective, and animals will inevitably require euthanasia to prevent further suffering. Preventative measures should be aimed at reducing stress and optimizing housing, husbandry, and diets. More research is warranted to elucidate the pathogenesis of MWS in captive callitrichids and to refine diagnostic and treatment methods. Full article

Regarding the ongoing global development of commercial ostrich husbandry, it is necessary to establish science-based recommendations for the appropriate treatment and welfare of ostriches. In this study, we determined the minimum housing area required for ostriches aged between 6 and 15 months. The planimetry revealed that the animals in this age group cover an average area of 0.31 m² to 0.57 m² with their bodies. To investigate the effects of space differences, the animals were divided into three groups and kept in 2.5 m², 5 m², and 10 m² of available space per animal. When comparing their weight and growth, a deficit was found in the animals that were provided with a housing area of 2.5 m². Furthermore, the animals exhibited differences in their integument and plumage score. The ostriches with the smallest housing area showed poorer scores in the plumage and integument than the animals with more space. At the conclusion of this study, the animals were slaughtered, and their carcass weights were compared. Similarly, lower carcass weights of the animals with a 2.5 m² barn area were observed. Prior studies have indicated that long-term stress leads to immune deficiencies associated with the inadequate development of the immune organs. However, a preliminary examination of the heart and spleen did not reveal any evidence for this. Further ethological studies are necessary to provide specific recommendations for minimum housing areas for ostriches. Full article

India, the leading producer and consumer of milk, houses 307 million cattle, nearly a third of the world’s total. A ban on cow slaughter exists in the majority of the Indian states. Intricately interweaved with the disputable yet publicly acknowledged sentiment of the cow’s sacrality, and with the animal’s utility value, the ban is capable of influencing the humans’ treatment of the dairy animals. Against this background, this research aims to evaluate the welfare state of the Indian dairy bovines by analyzing their welfare at various life phases using the Five Freedoms framework. A scoping review of the relevant published studies that met all inclusion criteria was conducted using bibliographic databases Google Scholar, Scopus, and ScienceDirect. Data were extracted and organized using Microsoft Excel, version 22H2. The literature reviewed demonstrates a loss of one or more freedoms in multiple phases of the bovines’ productive lives, and a parallel loss of two or more freedoms in their post-productive lives; indicating the role of religious values, legislation, and the animal’s economic utility in their treatment. This study highlights the role of culturally shaped human influences on dairy husbandry and creates grounds for studying human–bovine relationships through interdisciplinary lenses to generate culturally viable solutions to improve cattle welfare and promote a sustainable inter-species coexistence. Full article

The rhesus monkey (Macaca mulatta) is a non-human primate with a genome that is 93.5% identical to that of humans. Both species, therefore, have numerous phenotypical similarities in common. Consequently, this non-human primate is regularly studied in biomedical research. Not only does the rhesus monkey play an important role as an animal model for studying human disease, but it is also often featured in zoos, and there are substantial feral populations that live in Asia. Since they are exploited as research subjects, their appropriate housing and husbandry and the validation of obtained research data benefit from the comprehension of the rhesus monkey anatomy. Unexpectedly, the number of anatomical documents on the rhesus monkey are largely outnumbered by publications on the anatomy of domestic animals. In addition, the limited number of available anatomical books and atlases are, unfortunately, outdated, e.g., by presenting black-and-white photographs and using archaic nomenclature, or failing to cover the in-depth anatomy of various anatomical systems. Since state-of-the-art data on the rhesus monkey anatomy are requested by biomedical researchers and veterinarians responsible for the daily care of these captive animals, the present study describes the musculature of the foot of the rhesus monkey. It builds on a recently published manuscript on the topographical anatomy of the pelvic limb of this non-human primate. Full-color anatomical (stereomicroscopic) photographs are taken during layer-by-layer dissections of the feet of three rhesus monkeys. All the muscles, from the superficial to the deepest layer, are described using veterinary anatomical nomenclature and annotated on multipaneled figures. Although the foot musculature of the rhesus monkey largely parallels that of its human counterparts, the small number of dissimilarities should be recognized when extrapolating these research data. In addition, a solid understanding of the rhesus monkey anatomy by veterinarians can be valuable during medical interventions, such as surgery for foot injuries. Full article

Red wolves (Canis rufus) are the most critically endangered wolf globally, with over 95% of individuals living under human care. Gastrointestinal (GI) disease is one of the leading causes of death among adult red wolves under human care, yet links between management practices and this condition remain unclear. By integrating studbook data, survey-based husbandry information, veterinary records, and necropsy reports, our study explored the relationships between management practices and post-mortem diagnoses of GI mucosal disease among 36 adult red wolves between 2004 and 2022. For final diets fed for a minimum of 4 months before death, we report that wolves fed diets limited to only kibble were more likely to exhibit a greater severity of GI mucosal disease than those fed diets that integrated whole prey or other meat items. In addition, while living at their final facilities for a minimum of 9 months before death, wolves treated with a nonsteroidal anti-inflammatory drug exhibited more severe GI issues than those who were not. Conversely, we found no effect of housing and life history traits on GI health. This study highlights the importance of evaluating management and veterinary protocols on red wolf GI health. Full article

Background/Objectives: The emergence of the mobilized colistin resistance 1 (mcr-1) gene, which causes colistin resistance, is a serious concern in animal husbandry, particularly in pigs. Although antibiotic regulations in many countries have prohibited the use of colistin in livestock, the persistence and dissemination of this plasmid-mediated gene require effective and rapid monitoring. Therefore, a rapid, sensitive, and specific method combining recombinase polymerase amplification (RPA) with an in-house lateral flow assay (LFA) for the mcr-1 gene detection was developed. Methods: The colistin agar test and broth microdilution were employed to screen 152 E. coli isolates from pig fecal samples of five antibiotic-used farms. The established RPA-in-house LFA was validated with PCR for mcr-1 gene detection. Results: The RPA-in-house LFA was completed within 35 min (20 min of amplification and 5–15 min on LFA detection) at 37 °C. The sensitivity, specificity, and accuracy were entirely 100% in concordance with PCR results. No cross-reactivity was detected with seven common pathogenic bacteria or other mcr gene variants. Conclusions: Therefore, the in-house RPA-LFA serves as a point-of-care testing tool that is rapid, simple, and portable, facilitating effective surveillance of colistin resistance in both veterinary and clinical settings, thereby enhancing health outcomes. Full article

The Washington National Primate Research Center (WaNPRC) maintains the largest domestic breeding colony of pigtail macaques (Macaca nemestrina) in the United States, with animals housed in small to medium-sized social groups. As part of the animal care plan, a programmatic framework is utilized, which integrates clinical care with socialization considerations for nonhuman primates (NHPs). This framework encompasses the following areas: (1) socialization in the clinical setting; (2) positive reinforcement training (PRT); (3) measures to ensure proper identification and medication distribution; and (4) in-group treatments. The success of this framework is demonstrated by the high socialization rate for hospitalized animals (99.5% social pairing success), with the majority of clinical cases (95%) being treated in social groups. Ultimately, this framework seeks to buffer stressors when animals require clinical care or husbandry manipulations. Taken together, the above components foster an environment that provides a comprehensive approach to NHP medical and behavioral management. Full article

This study evaluated prevalence and risk factors in health, management, and biosecurity of bovine coronavirus (BCoV) in neonatal and weaned dairy calves on 125 dairy farms in Europe. Nasal and fecal swabs from neonatal calves, weaned calves, and fresh cows were analyzed for BCoV using RT-PCR, and blood and bulk milk samples were collected for BCoV antibody levels using ELISA. Multiple logistic regression models with random effects of herds were used to evaluate the herd health status, husbandry, management, and biosecurity associated with BCoV shedding (nasal and/or fecal PCR positive samples) in neonatal and weaned calves. BCoV was detected in 80% of herds and in 24% of neonatal calves, 23% of weaned calves, and 5% of fresh cows. The biosecurity scored on 109 dairies with Biocheck.Ugent was, on average, 60% (external score 71%, internal score 47%), and there was no clear association between various biosecurity measures on BCoV shedding in calves. Dry cow vaccination against BCoV reduced shedding in neonatal calves, whereas it was linked to increased shedding in weaned calves in these farms. Several husbandry factors, including nutrition (transition milk feeding and milk feeding levels) and management (group housing and weaning age), were associated with BCoV shedding in calves. Full article

Zoo enclosure design, and housing and husbandry protocols, will always be a compromise between what a species has evolved to do and what is possible to offer in a human-created environment. For some species, behaviours that are commonly performed in the wild may be constrained by husbandry practices that are used for ease or aesthetics or are accepted conventions. As zoos place more emphasis on positive animal welfare states, zoo enclosures should be scrutinised to check that what is provided, in terms of useful space, appropriate replication of habitat features, and maximal potential for natural behaviour performance, is relevant to the species and individuals being housed. For some species, zoos need to grapple with tough questions where the answer may not seem immediately obvious to ensure they are continuously improving standards of care, opportunities for the performance of species-typical behaviours, and advancing the attainment of positive welfare states. Determining the importance of flight, for example, and what this behaviour adds to the quality of life of a zoo-housed bird, is an important question that needs addressing to truly advance aviculture and how we determine bird welfare. This paper provides questions that should be answered and poses measures of what flight means to a bird, to provide evidence for the development and evolution of zoo bird housing. If we can devise some way of asking the animals in our care what they need, we can more firmly support decisions made that surround enclosure design, and housing decisions. Ultimately, this means gathering evidence on whether birds like to fly (e.g., from birds in training or demonstration activities) by applying mixed methods approaches of behavioural analysis, data on wild ecology, qualitative behavioural assessment, and cognitive bias testing to develop a robust suite of tools to address avian welfare considerations. Avian welfare scientists should attempt to define what meaningful flight is (i.e., flight that truly suggests a bird is flying) in order to support guidelines on aviary dimensions, space allowance, and welfare outputs from birds in both flighted and flight-restricted populations, and to determine what is most appropriate for an individual species. Changing the term “best practice” husbandry guidelines to “better practice” husbandry guidelines would instil the importance of regular review and reassessment of housing and management suitability for a species to ensure such care regimes remain appropriate. With an increasingly welfare-savvy public visiting zoos, it is essential that we seek more evidence to support and justify how birds are kept and ultimately use such evidence to enact changes to practices that are shown to infringe on avian welfare. Full article

This study investigates the captive environments of oriental small-clawed otter (OSO; Aonyx cinereus) cafés in Japan, aiming to identify discrepancies with established welfare guidelines. Improved management of these commercial facilities could raise awareness about the difficulties of keeping OSOs as pets and enhance their welfare. Utilizing the role of commercial facilities in public outreach, we consider that the rigorous implementation of proper care practices in these establishments could help mitigate the increasing demand for OSOs domestically and contribute to the conservation of the OSO. In this study, we focused on the critical aspects of the captive environment necessary to maintain the psychological well-being of OSOs and evaluated the captive environments of OSOs housed in animal cafés for OSO welfare using non-invasive methods. Based on zoo husbandry standards, it was found that there were deficiencies in aquatic environments and solitary housing conditions; however, the enrichment tools aligned with the guidelines. The results suggest that deviations from the recommended elements in the environment of OSOs in captivity are associated with a tendency of these OSOs to develop various diseases. It is deemed necessary to amend the regulations governing animal-handling businesses to include welfare criteria, and it is considered essential for each establishment to operate only after ensuring sufficient welfare for the OSOs. Full article