Search Results (1,825)

Background/Objectives: The concurrent use of smartphones and backpacks presents notable ergonomic challenges for students and young adults. However, the influence of walking speed on this dual-task biomechanical strain remains unclear. This study investigated how walking speed, backpack load, and gender affect postural alignment and neck–shoulder muscle activity during smartphone use. Methods: Thirty healthy university students (15 males and 15 females) were assessed using a motion capture system and surface electromyography to quantify postural alignment and muscle activity. Each participant completed twelve randomized conditions comprising three backpack loads (0%, 5%, and 10% of body weight) combined with four locomotor states (standing and walking at slow, normal, and fast speeds). Outcome measures included neck flexion, upper-trunk angle, lumbosacral angle, and normalized surface electromyography of the cervical erector spinae (CES) and upper trapezius (UTZ). A three-way ANOVA was used to evaluate main and interaction effects. Results: Increasing backpack load significantly increased neck flexion and upper-trunk angle while reducing the lumbosacral angle (all p < 0.001). Muscle activity rose proportionally with load, with UTZ activation nearly doubling from 10.7% to 21.1% maximum voluntary contraction (MVC) at 10% body weight. Faster walking increased lumbar flexion and elevated CES and UTZ activation (p < 0.05), while neck and upper-trunk postures remained stable across speeds. Females maintained more upright postures but exhibited higher muscle activity than males (p < 0.01). UTZ activation frequently exceeded the 15% MVC fatigue threshold during walking with backpacks. Conclusions: Walking speed critically modulates musculoskeletal strain during concurrent smartphone use and load carriage. The combined effects of backpack load and smartphone use can elevate neck–shoulder muscle demands, with UTZ activity occasionally approaching fatigue thresholds under moderate load and faster walking. Based on the current findings, backpack loads above 5% of body weight may increase the risk of neck–shoulder strain. Additionally, reducing smartphone use during fast walking may help prevent neck–shoulder fatigue and related musculoskeletal discomfort. Full article

Introduction: Manual therapists routinely evaluate changes in pain, movement, and function through clinical tests that support clinical reasoning. The Prone Hip Extension Test (PHET) is commonly used as a self-perturbation task to assess lumbopelvic control and hip motion patterns related to gait. Performing the PHET actively and passively may reveal how voluntary activation and passive structures influence joint kinematics and contribute to force production. This study aimed to compare active and passive PHET execution and investigate how initial (IP) and final hip positions (FP) correlate with lower-limb neuromuscular function. Methods: Seven healthy volunteers (24.3 ± 3.4 years; 173.1 ± 7.5 cm; 72.1 ± 9.5 kg) without musculoskeletal conditions participated. Hip kinematics were recorded using a 12-camera Qualisys Oqus system (200 Hz) with 22 reflective markers, processed in Qualisys Track Manager 2.13 and exported to Visual3D. Participants performed three PHET trials in both IP and FP, with mean an-gles considered for analysis. Knee isokinetic performance was assessed on a Biodex System 4 at 180°/s and 300°/s for flexion and extension. Results: Significant differences between active and passive PHET emerged in the FP for rotational movements bilaterally (p = 0.02) and in IP adduction/abduction for both hips (right p = 0.03; left p = 0.02). No side-to-side differences were observed. Passive FP of the right hip showed multiple significant correlations with isokinetic flexion and extension parameters at 180°/s and 300°/s, particularly with torque/body weight, acceleration and deceleration times, and agonist/antagonist ratios (ρ ranging from −0.86 to 0.90). Conclusions: Meaningful differences exist between active and passive PHET performance, especially in frontal-plane IP and rotational FP measures. Additionally, passive FP strongly correlates with several neuromuscular variables, suggesting that PHET kinematics may reflect lower-limb isokinetic function. Full article

Background/Objectives: Testosterone and cortisol are key regulators of metabolic, psychological, and physiological responses to environmental and lifestyle factors. This study aimed to analyze the relationship between free testosterone and cortisol concentrations and dietary patterns, stress levels, sleep quality, physical activity, and body composition in healthy young men (aged 18–35 years). Methods: This study included 40 volunteers who met our inclusion criteria. They underwent anthropometric measurements, body composition analysis, and biochemical determination of serum free testosterone and cortisol concentrations. Additionally, participants completed a set of validated questionnaires: a questionnaire regarding the frequency of consumption of specific foods and stimulants, a 3-day food diary, the PSS-10, the Holmes and Rahe Scale, the PSQI, and the Baecke Physical Activity Questionnaire. Results: Free testosterone concentration in blood was negatively correlated with body fat content and positively correlated with the percentage of energy, protein, fat, sodium, and folic acid requirements. Morning blood cortisol levels negatively correlated with body weight and height. Higher intakes of cholesterol, folic acid, and vitamin A resulted in statistically significant reductions in cortisol levels. A significant correlation was identified between poor sleep quality and low cortisol levels, particularly among men aged < 26 years. A positive correlation was also found between leisure-time physical activity and testosterone levels, particularly in the older group. Furthermore, a higher body weight and greater muscle mass were correlated with lower cortisol levels. Conclusions: These results provide a starting point for further research on neuroendocrine mechanisms in active individuals exposed to environmental stress. Full article

Food enrichment represents a novel feeding strategy for aquaculture. In the current study, juvenile Nibea albiflora (average weight 29.65 ± 0.13 g) were used and three feeding regimes (A—commercial diet; B—a diet comprising 90% commercial feed and 10% ice-fresh Palaemon gravieri; C—a diet consisting of 90% commercial diet, 5% ice-fresh Palaemon gravieri and 5% live Perinereis nuntia; named control group, Group 1, and Group 2) with comparable nutrient compositions: were designed to establish the food enrichment model and explore the effects of such feeding strategies on the fish. The cultivation period was 60 days, and the physiological, pathological, and RNA-seq analyses were performed to evaluate the effects. The results showed that the food enrichment feeding strategy significantly enhanced fish growth performance, immunity, and stress resistance without increasing the unit production cost (UPC). Furthermore, the tri-combined food feeding (C) was better than the two-combined food feeding (B). Liver transcriptomic analysis revealed that, in the comparison between the control group and Group 1, the up-regulated genes (alox15b, gng7, hif1a, ppara, and pla2g) and down-regulated genes (ins, gck, il4i1) influenced fish physiology and further improved growth. Similar to the comparison between the control group and Group 2, the major functional genes included ugt, nlrp3, mx1, col1a, gst (up-regulated), and map2k1, myc, mmp9, wnt7, socs3 (down-regulated) that participated in regulating the body growth, immunity, and stress resistance. The up-regulated genes (ins, mhc2, foxo3, ppara, and mx1) alongside the down-regulated genes (egfr, fos, cyc, myc, and mmp9) probably contributed to the enhanced efficacy of the tri-combined food feeding compared to the two-combined food feeding. In summary, this study demonstrates the beneficial effects of such a food enrichment model on the fish and provides empirical evidence supporting the implementation of the feeding strategies in the healthy culturing of the fish. Full article

The skull provides essential diagnostic features for species identification and sex determination. Cranial sexual dimorphism in guinea pigs (Cavia porcellus) is particularly important to understand, as these animals are frequently used in experimental research and veterinary practice, yet detailed morphometric evaluations remain limited. This study aimed to assess cranial size and shape variation between sexes using three-dimensional geometric morphometric (GM) methods. Computed tomography scans of 30 clinically healthy guinea pigs were used to reconstruct three-dimensional skull models. Twenty-one anatomical landmarks were digitized, and the dataset was subjected to generalized Procrustes analysis, followed by principal component analysis, Procrustes ANOVA, and regression-based allometric assessments. The results revealed pronounced sexual dimorphism. Males exhibited significantly larger centroid sizes and more robust cranial morphologies, whereas females presented smaller and more gracile skull forms. Procrustes ANOVA confirmed significant shape differences between sexes (p < 0.01), with size being the primary driver of morphological divergence. Body weight had a weaker but detectable influence on cranial variation, although its effect diminished after accounting for centroid size (R²: 0.085). These findings demonstrate that three-dimensional geometric morphometrics can effectively detect subtle patterns of cranial dimorphism in guinea pigs. Beyond their anatomical relevance, the results provide a valuable reference for veterinary sciences, taxonomy, and future biomedical research requiring precise morphological characterization. These findings show that three-dimensional geometric morphometrics can effectively detect subtle patterns of sexual dimorphism in cranial shape, which is particularly relevant for sex identification in skeletal collections and for the development of comparative anatomical databases in veterinary and experimental research. Full article

(1) Background: How variations of the push-up plus (PUP)—particularly changes in the base of support and scapular excursion—affect scapular muscle activation remains unclear. This study compared phase-specific electromyographic (EMG) activity of scapular muscles during four protraction–retraction exercises. (2) Methods: Twenty-six healthy male participants (age: 22.88 ± 1.45 years; height: 1.74 ± 0.05 m; weight: 77.31 ± 8.61 kg; body mass index (BMI): 25.61 ± 2.43 kg/m²) with Pilates experience performed four scapular protraction–retraction exercises under two base-of-support (quadruped vs. single-leg) and two movement-range (PUP vs. STD) conditions. Exercise order was randomized, and sufficient rest intervals were provided to minimize fatigue effects. Surface electromyography was recorded from six scapular muscles and normalized to maximal voluntary isometric contraction. The study was registered on CRIS (KCT0010032). (3) Results: Single-leg PUP showed the greatest serratus anterior (SA) activation, with increases of approximately 30% in protraction, 20–25% in isometric, and 15–20% in retraction. STD variations elicited higher trapezius activation, especially during large scapular excursions. The UT/SA ratio was significantly lower in PUP conditions (η²_p = 0.544), reflecting a more favorable stabilization pattern. (4) Conclusions: This experimental repeated-measures study demonstrates that combining single-leg support with traditional PUP meaningfully increases SA recruitment across all phases, whereas increased scapular range enhances trapezius engagement. These findings provide novel phase-specific insights into how PUP variations modulate closed-chain scapular stabilization and may assist clinicians in selecting targeted exercises. Interpretation should be limited to trained healthy males. Full article

Recently, a growing interest has been focused to the role of vitamin C in chronic diseases. Type 2 Diabetes Mellitus and the Metabolic Syndrome are complex, chronic disorders intrinsically linked by a common underlying element, such as chronic low-grade inflammation and excessive oxidative stress. Vitamin C, or ascorbic acid, is an essential water-soluble micronutrient and a highly potent non-enzymatic antioxidant that is critical for scavenging reactive oxygen species and maintaining cellular redox balance. It represents a cofactor for many enzymes, being involved in many biological functions, such as normal immune system functioning, catecholamine metabolism, dietary iron absorption, and collagen biosynthesis. Individuals with type 2 diabetes mellitus and metabolic syndrome frequently exhibit lower circulating and dietary vitamin C levels compared to healthy controls, a deficiency that may be associated with disease-related inflammation and higher body weight. In this sense, it has been shown that vitamin C improves skeletal muscle insulin sensitivity in experimental settings and modulates critical functions like vascular endothelial health. However, this potential is challenged by the fact that chronic hyperglycemia can interfere with the active cellular uptake and transport of vitamin C, potentially leading to relative intracellular deficiency in diabetic patients regardless of intake. It is interesting to note that different studies have demonstrated an inverse relationship between vitamin C concentrations and the prevalence of metabolic syndrome and type 2 diabetes. Vitamin C supplementation in people with diabetes and metabolic syndrome has controversial effects. While several studies indicate a significant reduction in fasting blood glucose or HbA1c, others revealed no significant effect on insulin resistance. This review aims to explore the pathophysiological role and therapeutic potential of vitamin C in type 2 diabetes and metabolic syndrome. Full article

In recent years, donkey milk has gained growing interest for its nutritional and therapeutic properties, stimulating both research and commercial interest. Monitoring udder health is essential to reduce production losses and ensure animal welfare. Despite its importance, information about the ultrasonographic anatomy of teat structures in lactating jennies is limited, and normal reference values are not well established. This study aimed to describe the ultrasonographic appearance of the teat and provide reference measurements in healthy lactating standard dairy jennies. Twenty-eight subjects were examined using a 13 MHz linear transducer, and longitudinal and transverse scans were performed to assess teat canal length, teat canal diameter, and cranial and caudal teat wall thickness. All measurements were repeatable and showed high bilateral symmetry. Teat canal diameter was positively correlated with the month of lactation (p < 0.05), whereas no significant associations were found with age or body weight. These findings establish normative ultrasonographic parameters for teat structures in jennies and highlight the progressive adaptation of the teat canal during lactation. Standardized measurements can support early detection of mammary gland pathologies, guide preventive management of mastitis, and improve udder health monitoring in donkey dairy farming. Full article

Food-Based Dietary Guidelines (FBDGs) are key tools for providing comprehensive, evidence-based recommendations for healthy eating using simple and understandable holistic diet approaches. This study aims to review national FBDGs in the Eastern Mediterranean Region (EMR), examine and compare their dietary and lifestyle recommendations, and explore the inclusion of environmental sustainability considerations. The Food and Agriculture Organization’s (FAO’s) global online FBDGs repository, Google Scholar, and ministry websites were consulted. Results showed that national FBDGs were available in 12 out of the 22 EMR countries (54.5%). The main purpose of these FBDGs was the promotion of general health by emphasizing the adoption of healthy dietary and lifestyle patterns. The various FBDGs categorized their foods into five, six, or seven different groups, the main differences being related to the inclusion of legumes and nuts as a distinct food group in some guides (instead of being in the group of meats and alternative), and to the featuring of fats and oils as a separate food group. In addition to promoting healthy and varied diets, all FBDGs in the region addressed food safety (100%), physical activity (100%), meal patterns (42%), maintenance of healthy body weight (83%), sleep (50%), and emotional well-being (17%). Aspects related to environmental protection were only considered by six national FBDGs (50%), of which only four addressed sustainability (33%). The study’s findings show that a minority of the FBDGs have undertaken revisions, which are particularly important in light of the global calls for promoting diets that are healthy for both people and the planet. Full article

This study addresses key challenges in intensive aquaculture, such as passive environmental control, high energy consumption, and neglected fish stress, through the development of a multi-objective environmental regulation system for crucian carp utilizing behavioral stress feedback. It combines YOLOv8s-FasterNet for behavior recognition, a specific growth rate model and an energy cost model to form an intelligent decision-making mechanism that maximizes the output–input ratio. In a 25-day experiment, the system showed strong performance. Final body weight and specific growth rate were comparable to the control group. Economically, the system achieved periodic profits that were 8.93, 1.43, and 1.03 times greater than those of traditional threshold control at external temperatures of 2 °C, 8 °C, and 14 °C, respectively, demonstrating significant energy savings. In terms of animal welfare, principal component analysis confirmed significantly lower stress-induced damage in the experimental group, with a comprehensive score (−0.036) closer to the initial healthy group (0.223) versus the control group (−0.348). These results indicate that the system successfully optimized both economic efficiency and fish health, providing a viable solution for intelligent aquaculture management. Full article

Pecans are a tree nut native to America with a rich content of unsaturated fatty acids, minerals, fiber, and a diverse array of bioactive components, including polyphenols, tocopherols, and phytosterols. This review summarizes variations in the phenolic composition of pecans from various parts of the world based on cultivar, maturity stage, postharvest storage, and processing. Additionally, the review delves into the bio-accessibility and bioavailability of bioactive components from pecans and their potential influence on diet quality, body weight, satiety, cardiometabolic, brain and gut health. Data from human clinical trials suggest that replacing foods/snacks with pecans improves overall diet quality and lipid profiles. However, inconsistent effects are observed on vascular function, glycemia, and inflammation. Body weight changes after pecan intake are reported as neutral, with promising results on satiety peptides and appetite regulation. Cognition and gut health are emerging areas of research with very limited data from both human and preclinical models, warranting further investigation. Overall, the current literature supports the cardiometabolic benefits of pecans within healthy dietary patterns. Future research should focus on well-controlled studies targeting at-risk populations to understand mechanistic endpoints such as metabolomics, microbiome, and vascular function assessments to substantiate the role of pecans in dietary guidance. Full article

Cardiovascular diseases (CVDs), including coronary artery disease (CAD), are the main causes of mortality and morbidity worldwide. The pathophysiology of CAD includes atherosclerosis, a chronic process leading to atherosclerotic plaque development. Clinical manifestations could be chronic, such as in the chronic coronary syndrome (CCS) scenario, or acute, such as acute coronary syndrome (ACS). The risk of subsequent cardiovascular (CV) events depends on the risk category defined by international guidelines. In particular, patients who have experienced a CV event requiring percutaneous coronary intervention (PCI) remain at heightened residual risk for subsequent events, despite advancements in standard-of-care strategies. Therefore, comprehensive residual risk management is essential in this population to mitigate ischemic risk. Secondary prevention includes different targets of treatments—hypertension, dyslipidemia, diabetes mellitus, body weight control, smoking habit reduction, and healthy lifestyle promotion. Nevertheless, there is a critical, unmet need for therapeutic strategies for this high-risk population. Growing evidence shows that atherogenic lipids and vascular inflammation drive residual risk after PCI, despite guideline-directed therapy. This review summarizes more recent evidence about secondary prevention focusing on optimal medical therapy (OMT), targeting lipids and inflammation for patients undergoing PCI. Full article

Background/Objectives: Advanced Practice Nurses (APNs) counsel patients regarding diet and exercise behaviors and serve as role models for health promotion and prevention of chronic disease. This study evaluated personal diet and exercise behaviors of APNs and their association with body mass index (BMI) as a biomarker of obesity and disease risk. Methods: APNs (N = 1268) self-reported height and weight, and answered four questions regarding personal diet and exercise. Based on BMI, they were grouped as normal weight (≤24.9 kg/m²) and overweight/obese (≥25.0 kg/m²). Results: The prevalence of overweight/obesity was 55%. The majority of APNs engaged in muscle strengthening exercises (53%) and consumed fruits and vegetables (62%), and protein foods and/or supplements (94%), while less than half engaged in moderate–vigorous physical activity (46%). Exercise behaviors (moderate–vigorous physical activity and muscle strengthening exercises) had a statistically significant impact on BMI. The predicted decrease in BMI due to participation in moderate–vigorous physical activity was 2.06 kg/m² and the predicted decrease due to muscle strengthening exercises was 1.35 kg/m². Diet behaviors (consumption of fruit, vegetables, and protein) were not found to have a significant impact on BMI. Conclusions: The prevalence of overweight/obesity among APNs in the United States is less than what is reported for the general adult population. Exercise behaviors, especially moderate–vigorous physical activity, significantly impact BMI and are associated with clinically meaningful differences. By comparison, healthy diet behaviors, including consumption of fruits, vegetables, and protein, are relatively widespread among advanced practice nurses but do not appear to significantly impact BMI. Full article

Background: Neurofilament light chain (NfL) is a well-established biomarker of neuroaxonal damage, detectable in serum through immunoassays. Its potential relevance in psychiatric conditions, including anorexia nervosa (AN), is currently under investigation. This study aims to quantify serum NfL levels in individuals with AN, evaluate their correlation with autoantibodies detection, and critically examine the specificity of NfL as a biomarker in this context. Methods: A total of 100 participants were enrolled, comprising 50 individuals diagnosed with AN and 50 age-matched, normal-weight controls. Serum concentrations of NfL and immunoglobulin G (IgG) antibodies reactive to hypothalamic antigens were measured using validated immunoassay techniques. Results: Serum NfL concentrations were markedly higher in the AN group compared to healthy controls. Interestingly, NfL levels tended to decrease with longer disease duration and with the recovery of body mass index (BMI), indicating a possible association between clinical improvement and reduced neuroaxonal damage. Furthermore, the results confirmed the presence of anti-hypothalamic autoantibodies and revealed a positive correlation between their levels and serum NfL concentrations. Conclusions: Clinical remission in AN appears to be linked to a decrease in both markers neuronal damage and hypothalamic autoimmunity. However, as elevated serum NfL is observed across a spectrum of neurological and psychiatric disorders, its specificity as a biomarker for AN should be further investigated. While NfL may reflect neuroaxonal injury in AN, its interpretation should be contextualized within a broader clinical and immunological framework. Full article

Background: Plant-based diets are associated with reduced cardiometabolic risk, yet the influence of lifestyle behaviors on these benefits remains insufficiently understood. Objective: To assess the combined impact of dietary patterns and lifestyle behaviors on body composition, lipid profiles, and inflammatory biomarkers in healthy young adults. Methods: In this cross-sectional study, 155 participants aged 18–39 years were categorized into four dietary groups: vegans (n = 48), vegetarians (n = 49), pescatarians (n = 23), and omnivores (n = 35). Body composition was measured using bioelectrical impedance analysis. Blood samples were analyzed for lipid parameters, apolipoproteins, lipoprotein(a), and inflammatory markers (IL-6, TNF-α, and hsCRP). Participants were further stratified based on behavioral factors, including physical activity, sleep duration, smoking, and alcohol consumption. Results: Vegans demonstrated the lowest body fat and visceral adipose tissue, along with the second-highest skeletal muscle mass. Significant intergroup differences were observed in total cholesterol (p = 0.032), HDL-C (p = 0.006), and triacylglycerols (p = 0.005). Among vegans, suboptimal lifestyle behaviors were associated with elevated LDL-C, non-HDL-C, and homocysteine levels (p < 0.05). Positive correlations were identified between ApoB and BMI (r = 0.517) and between IL-6 and waist–to–hip ratio (ρ = 0.499). Conclusions: A vegan diet, when combined with healthy lifestyle behaviors, is associated with favorable body composition and lipid profiles. Regardless of dietary pattern, maintaining a healthy body weight and minimizing visceral adiposity are essential for reducing cardiovascular and inflammatory risk. These research findings underscore the importance of integrating high-quality plant-based diets with lifestyle modifications and advanced modeling approaches. Full article