Search Results (11)

Coral reefs experience numerous natural and anthropogenic environmental gradients that alter biophysical conditions and affect biodiversity. While many studies have focused on drivers of reef biodiversity using traditional diversity metrics (e.g., species richness, diversity, evenness), less is known about how environmental variability may influence functional diversity. In this study, we tested the impact of submarine groundwater discharge (SGD) on taxonomic and functional diversity metrics in Mo‘orea, French Polynesia. SGD is the expulsion of terrestrial fresh or recirculated seawater into marine environments and is associated with reduced temperatures, pH, and salinity and elevated nutrient levels. Using a regression approach along the SGD gradient, we found that taxon and functional-entity richness displayed unimodal relationships to SGD parameters, primarily nitrate + nitrite and phosphate variability, with peak richness at moderate SGD for stony coral and the full benthic community. Macroalgae showed this unimodal pattern for functional-entity but not taxonomic richness. Functional community composition (presence and abundance of functional entities) increased along the gradient, while taxonomic composition showed a nonlinear relationship to SGD-related parameters. SGD is a common feature of many coastal ecosystems globally and therefore may be more important to structuring benthic functional diversity than previously thought. Further, studying community shifts through a functional-trait lens may provide important insights into the roles of community functions on ecosystem processes and stability, leading to improved management strategies. Full article

The sorting of machine-picked fresh tea leaves after mechanized harvesting remains a challenge because of the complex morphological characteristics and physicochemical properties of fresh tea leaves. First, the recognition results of four types of models, namely, YOLOv5, YOLOv3, Fast RCNN, and SSD, were compared. It was found that YOLOv5, with guaranteed recognition accuracy, had a recognition speed of 4.7 ms/frame (about four times that of the second ranked YOLOv3). Therefore, this study presents a novel fresh tea leaf sorting system that provides rapid and high-precision multi-channel sorting for four grades of tea leaves using a tea leaf recognition model based on the You Only Look Once (YOLOv5) deep learning model. Subsequently, a raw dataset, consisting of 6400 target images of different grades and different moisture contents, was used to evaluate three different optimization methods. Among these, the Stochastic Gradient Descent (SGD) optimization method was found to provide the best model training results with an average recognition accuracy of 98.2%. In addition, the recognition efficacy of the recognition model was found to be positively correlated with the gradient coverage of tea’s moisture content in the training set. Theoretical analysis was then conducted, along with the experimental investigation of the air-blowing force on the fresh tea leaves in the sorting process, with 30° determined to be the optimal air-blowing angle. Finally, the overall results showed that the construction of the full moisture content training set enabled a model recognition accuracy of up to 88.8%, a recall of 88.4%, a recognition speed of 4.7 ms/frame, and an overall sorting accuracy of 85.4%. This result is promising for multi-channel sorting of fresh tea leaf grades in complex situations, and as such provides a strong basis for the application of tea leaf sorting equipment. Full article

Quantifying the freshwater component of submarine groundwater discharge (SGD) is critical in the analysis of terrestrial influences on marine ecosystems and in assessing the water budget and groundwater recharge of coastal aquifers. In semi-arid to arid settings, this quantification is difficult because low SGD rates translate into low concentrations of groundwater solutes in coastal waters. In this study, fresh SGD (FSGD) was quantified for Toyon Bay on Catalina Island, California, for wet and dry seasons using a combination of radon and salinity mass balance models, and the results were compared to watershed-specific groundwater recharge rates obtained from soil water balance (SWB) modeling. Calculated FSGD rates vary only slightly with season and are remarkably similar to the recharge estimates from the SWB model. While sensitivity analyses revealed FSGD estimates to be significantly influenced by uncertainties in geochemical variability of the groundwater end-member and fluctuations of water depth, the results of this study support the SWB-model-based recharge rates. The findings of this study highlight the utility of the radon-and-salinity-mass-balance-based FSGD estimates as groundwater recharge calibration targets, which may aid in establishing more refined sustainable groundwater yields. Full article

Catharanthus roseus (L.) G. Don is a plant belonging to the genus Catharanthus of the Apocynaceae family. It contains more than one hundred alkaloids, of which some exhibit significant pharmacological activities. Chitooligosaccharides are the only basic aminooligosaccharides with positively charged cations in nature, which can regulate plant growth and antioxidant properties. In this study, the leaves of Catharanthus roseus were sprayed with chitooligosaccharides of different molecular weights (1 kDa, 2 kDa, 3 kDa) and different concentrations (0.01 μg/mL, 0.1 μg/mL, 1 μg/mL and 10 μg/mL). The fresh weights of its root, stem and leaf were all improved after chitooligosaccharides treatments. More importantly, the chitooligosaccharides elicitor strongly stimulated the accumulation of vindoline and catharanthine in the leaves, especially with the treatment of 0.1 μg/mL 3 kDa chitooligosaccharides, the contents of them were increased by 60.68% and 141.54%, respectively. Furthermore, as the defensive responses, antioxidant enzymes activities (catalase, glutathione reductase, ascorbate peroxidase, peroxidase and superoxide dismutase) were enhanced under chitooligosaccharides treatments. To further elucidate the underlying mechanism, qRT-PCR was used to investigate the genes expression levels of secologanin synthase (SLS), strictosidine synthase (STR), strictosidine glucosidase (SGD), tabersonine 16-hydroxylase (T16H), desacetoxyvindoline-4-hydroxylase (D4H), deacetylvindoline-4-O-acetyltransferase (DAT), peroxidase 1 (PRX1) and octadecanoid-responsive Catharanthus AP2-domain protein 3 (ORCA3). All the genes were significantly up-regulated after chitooligosaccharides treatments, and the transcription abundance of ORCA3, SLS, STR, DAT and PRX1 reached a maximal level with 0.1 μg/mL 3 kDa chitooligosaccharides treatment. All these results suggest that spraying Catharanthus roseus leaves with chitooligosaccharides, especially 0.1 μg/mL of 3 kDa chitooligosaccharides, may effectively improve the pharmaceutical value of Catharanthus roseus. Full article

‘Esmeralda’ is an orange fleshed peach cultivar primarily used for juice extraction and secondarily used for the fresh fruit market. Fruit yield and quality depend on several local environmental and managerial factors, mainly on nitrogen, which must be balanced with other nutrients. Similar to other perennial crops, peach trees show carryover effects of carbohydrates and nutrients and of nutrients stored in their tissues. The aims of the present study are (i) to identify the major sources of seasonal variability in fruit yield and qu Fruit Tree Department of Federal University of Pelotas (UFPEL), Pelotas 96010610ality; and (ii) to establish the N dose and the internal nutrient balance to reach high fruit yield and quality. The experiment was conducted from 2014 to 2017 in Southern Brazil and it followed five N treatments (0, 40, 80, 120 and 160 kg N ha⁻¹ year⁻¹). Foliar compositions were centered log-ratio (clr) transformed in order to account for multiple nutrient interactions and allow computing distances between compositions. Based on the feature ranking, chilling hours, degree-days and rainfall were the most influential features. Machine learning models k-nearest neighbors (KNN) and stochastic gradient decent (SGD) performed well on yield and quality indices, and reached accuracy from 0.75 to 1.00. In 2014, fruit production did not respond to added N, and it indicated the carryover effects of previously stored carbohydrates and nutrients. The plant had a quadratic response (p < 0.05) to N addition in 2015 and 2016, which reached maximum yield of 80 kg N ha⁻¹. In 2017, harvest was a failure due to the chilling hours (198 h) and the relatively small number of fruits per tree. Fruit yield and antioxidant content increased abruptly when foliar clr_Cu was >−5.410. The higher foliar P linearly decreased total titratable acidity and increased pulp firmness when clr_P > 0.556. Foliar N concentration range was narrow at high fruit yield and quality. The present results have emphasized the need of accounting for carryover effects, nutrient interactions and local factors in order to predict peach yield and nutrient dosage. Full article

The mud crab (Scylla serrata) is an economically important species found in the mangroves and estuaries of tropical countries and is a popular seafood product in the coastal areas of Southeast Asian markets. The aim of this study is to identify factors affecting consumer preference of mud crabs, and to understand consumers’ willingness to pay (WTP) for these factors in a Singapore market where imported mud crabs from other Asian countries are sold. The results showed that the country of origin matters to participants, affecting purchasing decisions, and consumers were willing to pay approximately 16.48 SGD (11.49 USD, the average price of four shops: 35.55 USD/kg for one mud crab) more if the mud crabs were from Sri Lanka if compared with Indonesian or Cambodian mud crabs. Moreover, consumers were willing to pay 25.08 SGD (17.48 USD) more if the mud crabs were alive and 13.40 SGD (9.34 USD) less if the mud crabs were frozen compared with fresh, but not alive, mud crabs. Furthermore, consumers’ preference in mud crab was associated with the consumers’ religion. Some groups of consumers preferred female mud crabs with eggs over male mud crabs, while other groups preferred male crabs. The results identified diverse consumer preference of mud crabs and, therefore, could provide new insights that are useful for sustainable consumption of mud crabs in the region. Full article

Submarine groundwater discharge (SGD) is an important component of many coastal environments and hydrologic processes, providing sources of nutrients to marine ecosystems, and potentially, an important source of fresh water for human populations. Here, we use a combination of unpiloted aerial systems (UAS) thermal infrared (TIR) imaging and salinity measurements to characterize SGD on the remote East Polynesian island of Rapa Nui (Easter Island, Chile). Previous research has shown that coastal freshwater seeps are abundant on Rapa Nui and strongly associated with the locations of ancient settlement sites. We currently lack, however, information on the differential magnitude or quality of these sources of fresh water. Our UAS-based TIR results from four locations on Rapa Nui suggest that locations of variably-sized SGD plumes are associated with many ancient settlement sites on the island and that these water sources are resilient to drought events. These findings support previous work indicating that ancient Rapa Nui communities responded to the inherent and climate-induced hydrological challenges of the island by focusing on these abundant and resilient freshwater sources. Our results highlight the efficacy of using UAS-based TIR for detecting relatively small SGD locations and provide key insights on the potential uses of these water sources for past and current Rapa Nui communities. Full article

Submarine Groundwater Discharge (SGD) represents a significant mode of chemical transport to water bodies, making it an important flux to understand. Small Unmanned Aircraft Systems-deployed thermal infrared sensors (sUAS-TIR) provide a financially and logistically inexpensive means of identifying SGD source zones and quantifying SGD thermal infrared (TIR) plume areas over regional scales at high spatial resolutions. sUAS-TIR additionally offers the unique capability of high temporal resolution measurements of SGD. As a developing science application, the use of sUAS-TIR to image SGD requires substantial background knowledge. We present a proposed methodological construct for implementing a sUAS-TIR program for SGD-TIR data gathering, with applications extending to other research fields that can benefit from airborne TIR. Several studies have used airborne TIR in combination with empirical SGD flux measurements to quantify SGD, reporting a consistently strong regression between SGD flux and SGD TIR plume area. We additionally discuss novel research opportunities for sUAS-TIR technologies, as applied to SGD flux. The combination of high spatial and temporal resolution capabilities, at relatively low costs, make sUAS-TIR a promising new technology to overcome the scaling challenges presented by empirical studies and modeling of SGD fluxes, and advance our understanding of the controls on SGD fluxes. Full article

Submarine groundwater discharge (SGD) is evidenced around Taiwan, but the seasonal/temporal changes of SGD have not been fully examined. Here, we report a time-series investigation of SGD into a tide-dominated coastal wetland, the Gaomei Wetland, located to the south of the Da-Chia River’s mouth, western Taiwan, by using environmental tracers (²²²Rn, ²²⁴Ra_ex, ²²⁸Ra, δD, and δ¹⁸O). Our results showed that regardless of dry and wet seasons, the ²²²Rn activities in coastal waters were high at low tide but low at high tide. It represents the continuous input of ²²²Rn-enriched groundwater. However, the ²²⁴Ra_ex and ²²⁸Ra activities showed seasonal changes with tide conditions. In the dry season, the ²²⁴Ra_ex and ²²⁸Ra activities in coastal waters were low at low tide but high at high tide; whereas in the wet season, an opposite relation was observed with quite high ²²⁴Ra_ex and ²²⁸Ra activities in the low-tide waters. Coupled with the lower δD and δ¹⁸O values of coastal and pore waters in the dry season, in comparison to those in the wet season, it is suggested that these phenomena probably reflected a seasonal difference in the main SGD component with fresh SGD in the dry season, but saline ones in the wet season. Based on a ²²²Rn mass balance model, the estimated SGD fluxes into the Gaomei Wetland varied with tidal fluctuations and ranged from 0.2 to 25 cm d⁻¹ and from 0.1 to 47 cm d⁻¹ for the dry and wet seasons, respectively. A slightly high SGD flux occurring during the wet season at spring tide, implied a stronger tidal pumping coupled with a larger hydraulic gradient between land and sea. In this study, we demonstrated that the variation of SGD into the Gaomei Wetland is not only controlled by the seasonal changes of groundwater recharge, but also by the tidal pumping process. Full article

The paper presents an improved approach for investigating submarine groundwater discharge (SGD) based on radon mapping and radon mass balancing in the coastal sea. While the use of radon as an environmental tracer in SGD studies is well-established, we identified based on our longstanding experience six methodical shortcomings of the conventional approach and suggest corresponding developments. The shortcomings include: (1 and 2) inadequate consideration of both detection equipment response delay and influence of tidal stage; (3 and 4) incorrect quantification of radon losses, due to offshore mixing and degassing resulting in a potentially incorrect radon mass balance; (5) inaccurate determination of the terrestrial groundwater endmember, due to inhomogeneous radon distribution in the coastal aquifer; and (6) difficulties in distinguishing between discharged fresh groundwater and recirculated seawater. The improved approach is practically demonstrated in a step by step manner in a large-scale field study, which was carried out in False Bay (South Africa) and which consisted of two parts, namely (i) qualitative SGD localization along the entire False Bay coastline based on coastal radon distribution patterns and (ii) quantitative SGD investigation within a defined coastal area of interest (AOI) based on a radon mass balance (RMB). The plausibility of the AOI related results was evaluated by a hydrogeological model, used for qualitative SGD localization, and a hydrological model, applied for estimating groundwater recharge within the AOI catchment. Full article

Submarine groundwater discharge (SGD) consists of fresh submarine groundwater discharge (FSGD) and recirculated submarine groundwater discharge (RSGD). In this study, we conducted simultaneous 25-hour time-series measurements of short-lived ²²²Rn and ²²⁴Ra activities at two sites with differing SGD rates in the central Seto Inland Sea of Japan to evaluate SGD rates and their constituents. At both sites, we also quantified the total SGD, FSGD, and RSGD using a seepage meter to verify the water fluxes estimated with ²²²Rn and ²²⁴Ra. SGD rates estimated using ²²²Rn and ²²⁴Ra at the site with significant SGD approximated the total SGD and RSGD measured by the seepage meter. However, SGD rates derived using ²²²Rn at the site with minor SGD were overestimated, since ²²²Rn activity at the nearshore mooring site was lower than that in the offshore area. These results suggest that the coupling of short-lived ²²²Rn and ²²⁴Ra is a powerful tool for quantification of FSGD and RSGD, although it is important to confirm that tracer activities in coastal areas are higher than those in offshore. Full article