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Keywords = destabilase

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19 pages, 616 KiB  
Article
Blind Spot for Pioneering Farmers? Reflections on Dutch Dairy Sustainability Transition
by Anne-Charlotte Hoes and Lusine Aramyan
Sustainability 2022, 14(17), 10959; https://doi.org/10.3390/su141710959 - 2 Sep 2022
Cited by 4 | Viewed by 3149
Abstract
This study explores the questions of how to govern the transition towards sustainable farming in a responsible and inclusive manner by exploring the Dutch dairy case. Sustainability transition is about fundamental social–technical changes to address the grand challenges that society faces today. It [...] Read more.
This study explores the questions of how to govern the transition towards sustainable farming in a responsible and inclusive manner by exploring the Dutch dairy case. Sustainability transition is about fundamental social–technical changes to address the grand challenges that society faces today. It includes breaking down and phasing out unsustainable practice as well as scaling up sustainable alternatives. Transition literature argues that governments should implement a mix of transition tasks to give direction, support the new and destabilase the unsustainable. In addition, market-based instruments (MBIs) and policy interventions rewarding sustainable farming stimulate transition. This study illustrates that strong and prolonged pressure of not meeting international environmental agreements triggered the implementation of stronger policy interventions that destabilize the unsustainable. However, less policy attention seems to be given to supporting the “new”, such as pioneering alternative farmers who develop sustainable alternatives to mainstream farming. To achieve more responsible and inclusive sustainability transitions, it is important to implement tailor-made policies that support pioneering alternative farmers who are already taking steps in developing sustainable farms which, in addition to food, provide ecological and other benefits to community. Full article
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14 pages, 1999 KiB  
Article
Recombinant Destabilase from Hirudo medicinalis Is Able to Dissolve Human Blood Clots In Vitro
by Pavel Bobrovsky, Valentin Manuvera, Izolda Baskova, Svetlana Nemirova, Alexandr Medvedev and Vassili Lazarev
Curr. Issues Mol. Biol. 2021, 43(3), 2068-2081; https://doi.org/10.3390/cimb43030143 - 20 Nov 2021
Cited by 6 | Viewed by 14855
Abstract
Leeches are amazing animals that can be classified as conditionally poisonous animals since the salivary cocktail they produce is injected directly into the victim, and its components have strictly defined biological purposes, such as preventing blood clot formation. Thrombolytic drugs are mainly aimed [...] Read more.
Leeches are amazing animals that can be classified as conditionally poisonous animals since the salivary cocktail they produce is injected directly into the victim, and its components have strictly defined biological purposes, such as preventing blood clot formation. Thrombolytic drugs are mainly aimed at treating newly formed blood clots. Aged clots are stabilized by a large number of isopeptide bonds that prevent the action of thrombolytics. These bonds are destroyed by destabilase, an enzyme of the leech’s salivary glands. Here, we conducted a pilot study to evaluate the feasibility and effectiveness of the use of destabilase in relation to blood clots formed during real pathological processes. We evaluated the isopeptidase activity of destabilase during the formation of a stabilized fibrin clot. We showed that destabilase does not affect the internal and external coagulation cascades. We calculated the dose–response curve and tested the ability of destabilase to destroy isopeptide bonds in natural blood clots. The effect of aged and fresh clots dissolving ability after treatment with destabilase coincided with the morphological characteristics of clots during surgery. Thus, recombinant destabilase can be considered as a potential drug for the treatment of aged clots, which are difficult to treat with known thrombolytics. Full article
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