Search Results (199)

Objective: The objective of this study is to examine the detailed pregnancy and neonatal outcomes of women without male partners undergoing intrauterine insemination (IUI) compared to women with male partners. Methods: This is a retrospective cohort study of all patients who completed an IUI cycle from 2017 to 2023. 2414 cycles were included in the study: 149 cycles for women without male partners (including single and lesbian women) and 2265 cycles for women with male partners. Primary outcomes were the rates of clinical pregnancy, miscarriage, and live birth. Secondary outcomes were obstetric complication rates and neonatal outcomes. Results: Women without male partners undergoing IUI were significantly older than the reference cohort (median age 42 years versus 38 years, p < 0.0001). 84.1% of women without male partners did not have a diagnosis of the common causes of female infertility. Both cohorts had similar cycle characteristics and number of IUI cycles until pregnancy and live birth. The mean clinical pregnancy rate per cycle for women without male partners was 11.4% versus 12.5% for the reference group (p = 0.56), and the mean live birth rate was 8.1% versus 8.2% (p = 0.95). Multiple pregnancy, cumulative pregnancy, and clinical miscarriage rates were also similar. Similarities persisted after adjusting for confounders: age, BMI, race, and infertility diagnosis. Importantly, there were no statistically significant differences in obstetric complications (such as hypertensive disorders of pregnancy, gestational diabetes, placental disorders) and neonatal outcomes. Conclusions: Compared to women with male partners undergoing IUI, women without male partners had similar rates of clinical pregnancy (per cycle and cumulative), miscarriage, and live birth; there were no significant differences in obstetric complications or neonatal outcomes. Full article

The article presents the results of a study on heat transfer within the engine block of the Eco Arrow 3 prototype vehicle, developed for participation in Shell Eco-marathon competitions. The main objective of these events is to minimize fuel consumption during track races, which leads to a specific driving strategy characterized by frequent engine shut-downs and restarts. Such a driving style introduces challenges not typically encountered in conventional vehicles, including the need to maintain the engine within an optimal temperature range. In this work, several geometric variants of cylinder finning were investigated with respect to their influence on cooling, heating, and heat accumulation. Four configurations of finning were analysed: the original fins with a height of h = 15 mm, a cylinder with fins completely removed (h = 0 mm), and two intermediate variants with fin heights of 5 mm and 10 mm. The original and finless cylinders were studied both experimentally and numerically, while the intermediate variants were analysed solely using numerical methods. A comparison between experimental and numerical results showed satisfactory agreement in terms of maximum temperatures, with differences of approximately 10–15 °C. Considering the specific operating conditions of such an engine, characterized by irregular on–off cycles, the numerical analysis indicated that fins with a height of h = 10 mm provide the most favourable balance, ensuring that the engine remains within the optimal temperature range required to achieve minimal fuel consumption. Full article

In road cycling, developing bike handling skills can prevent crashes and falls. Nevertheless, bike handling remains largely unexplored in the world of road cycling. The goal of this research was to develop a methodology to assess bike handling during races and training by estimating the rider–bicycle roll angle and road-plane accelerations from global positioning system (GPS) data only. A multi-dimensional bike-rider mathematical model was included in an optimal control framework to follow a reference trajectory generated from GPS data points. Estimated variables and experimental data collected with a cost-effective setup showed good agreement, i.e., root mean square error (RMSE) of 12° and 0.1 g for roll angle and both longitudinal and lateral accelerations, respectively, in the worst-case scenarios. This methodology might allow for the estimation of key bike handling variables during fast segments with cost-effective instrumentation. It can therefore constitute a tool for objectively assessing bike handling in road cycling training and racing. Full article

In this paper, we analyze the possibilities of optimizing the driving strategy for energy-efficient electric vehicles competing in the Shell Eco-marathon race. The base method we already developed and successfully applied for several years—winning the Urban Concept Battery Electric competition of the 2022, 2023, and 2024 Shell Eco-marathon races—was further tested, with small modifications to our optimization method. We only used an optimizer tool based on a genetic algorithm. We were interested in determining how a modification to the minimalization problem could help our optimizer find the best driving cycle to reach the minimum energy consumption. We successfully applied the modification to our method at the 2025 competition, where we beat our own record and proved its practical applicability. Full article

This study aimed to characterize and analyse the kinematic parameters and muscle activity of swimmers in open water swimming (OWS). Nine male swimmers (age: 25.4 ± 11.9 years; body mass: 75.9 ± 9.0 kg; height: 180.7 ± 6.7 cm; and arm span: 185.6 ± 10.3 cm) were evaluated in an open environment (lake), performing 5 m × 1000 m at maximum intensity, with a rest of 30 s every 1000 m. For kinematical analyses, the stroke rate (SR), swimming velocity (v), stroke length (SL), and stroke index (SI) were calculated. Surface EMG data were recorded in seven muscles—upper trapezius (UP); latissimus dorsi (LD); pectoralis major (PM); posterior deltoid (PD); anterior deltoid (AD); triceps brachii (TB); and biceps brachii (BB)—for the underwater and recovery phases of the stroke. SL (F = 3.41, p = 0.06, η² = 0.30) and SI (F = 3.29, p = 0.08, η² = 0.29) changed along the covered distances, and SR (F = 1.54, p = 0.24, η² = 0.16) increased, especially in the last 1000 m (32.5 ± 3.0 cycles-min⁻¹). AD was highly activated in recovery, showing statistical differences from the beginning (p ≤ 0.01) to the end of the race (p = 0.03). The TB muscle was mostly recruited in the underwater phase, from the start (p ≤ 0.01) to the finish (p = 0.03), showing a significant difference in each lap, with a large effect. LD showed significant differences in muscle activation, from 1000 m (p ≤ 0.01) with a huge effect, to 5000 m (p ≤ 0.01), with a large effect. These results suggested that the UT and AD muscles had higher activity in recovery than the underwater phase, and TB and LD were higher in the underwater phase. Full article

Autism spectrum disorder (ASD) deals with several symptoms, including language and speech impairment and developmental delays. The main brain regions affected could be the prefrontal cortex (PFC) or the temporal lobe. The detrimental features could include oxidative stress, mitochondrial dysfunction, and neuroinflammation. Most often, these phenomena are interrelated and can lead to one another, creating a vicious cycle. They also influence the regulation of certain genes involved in the pathogenesis of ASD or related behavior. In the brain regions prone to these detrimental features, a cascade of free radicals, inflammatory cytokines, and mitochondrial energy disruptions is initiated. These actions during the prenatal or developmental stage of the child potentially lead to ASD symptomatic features, such as social isolation, communication difficulty, speech and language impairment, cognitive dysfunction, and intellectual disability. The more recent theories, including genetics, epigenetics, and the gut–brain axis, have been demonstrated to play a greater role in ASD pathology, often being associated with the more common ones as mentioned above. We also introduced some of the neurological disorders possessing shared genetic and behavioral traits with ASD. Many genes playing a role in ASD-like features and their potential targeted drugs were explained briefly. However, there are limited therapeutic options, and molecular pathways related to this disorder are less explored. Currently, researchers and therapists are racing to uncover a concrete remedy. This review also provides a brief outline of potential antioxidant, mitochondrial, and anti-inflammatory therapies. We finally included some novel strategies to diagnose and manage autistic pathology and symptoms. Full article

Rowing is a cyclic sport that consists of repetitive biomechanical actions, with performance being influenced by the balance between propulsive and resistive forces. The current study aimed to assess the relationships between intracycle velocity variation (IVV) and key biomechanical and performance variables in male and female single scullers. Twenty-three experienced rowers (10 females) completed a 2000 m rowing competition, during which boat position and velocity were measured using a 15 Hz GPS, while cycle rate was derived from the integrated triaxial accelerometer sampling at 100 Hz. From these data, it was possible to calculate distance per cycle, IVV, the coefficient of velocity variation (CVV), and technical index values. Males presented higher mean, maximum and minimum velocity, distance per cycle, CVV, and technical index values than females (15.40 ± 0.81 vs. 13.36 ± 0.88 km/h, d = 0.84; 21.39 ± 1.68 vs. 18.77 ± 1.52 km/h, d = 1.61; 11.15 ± 1.81 vs. 9.03 ± 0.85 km/h, d = 1.45; 7.68 ± 0.32 vs. 6.89 ± 0.97 m, d = 0.69; 14.13 ± 2.02 vs. 11.64 ± 1.93%, d = 2.06; and 34.25 ± 4.82 vs. 26.30 ± 4.23 (m²/s·cycle), d = 4.56, respectively). An association between mean velocity and intracycle IVV, CVV, and cycle rate (r = 0.68, 0.74 and 0.65, respectively) was observed in males but not in female single scullers (which may be attributed to anthropometric specificities). In female single scullers, mean velocity was related with distance per cycle and was associated with technical index in both males and females (r = 0.76 and 0.66, respectively). Despite these differences, male and female single scullers adopted similar pacing strategies and CVV remained constant throughout the 2000 m race (indicating that this variable might not be affected by fatigue). Differences were also observed in the velocity–time profile, with men reaching peak velocity first and having a faster propulsive phase. Data provided new information on how IVV and CVV relate to commonly used biomechanical variables in rowing. Technical index (r = 0.87): distance per cycle was associated with technical index in both males and females (r = 0.76 and 0.66, respectively). Future studies should include other boat classes and other performance variables such as the power output and arc length. Full article

Background: Previous research has linked hypertensive disorders of pregnancy (HDP) and long-term cardiovascular disease (CVD) with assisted reproductive technology (ART). It is not clear whether this reflects the background population cardiovascular profiles or whether ART independently increases the long-term risk for CVD and alters cardiovascular function. Furthermore, CVD has been associated with pathological cardiovascular function before and after the establishment of the disease. The aim of this study was to compare cardiac function in women attending for ART between those who had previous treatment and those who had not after controlling for demographic characteristics which have been shown to affect cardiovascular function. Methods: This was a prospective observational cohort study at a London fertility clinic. Women were consecutively enrolled between May 2021 and March 2022. Maternal demographics and cardiac function using transthoracic echocardiography were assessed before the current treatment cycle in the mid-luteal phase of the menstrual cycle. Maternal demographics included age, body mass index, smoking, race, and parity. Cardiovascular parameters included blood pressure and indices of left-ventricular systolic and diastolic function. Differences between cardiac variables after controlling for maternal demographics and history of previous ART were assessed by multivariate linear regression. Results: There were 232 healthy women who agreed to participate in the study; of those, 153 (58%) had undergone previous ART. After controlling for maternal demographic characteristics, previous assisted reproductive technology was not an independent predictor of cardiac function. Conclusions: Previous ART is not associated with significant changes in cardiac function. Full article

Background: Circadian rhythms regulate critical cellular processes, including DNA repair and cell cycle dynamics, potentially influencing the effectiveness of radiotherapy (RT). This study evaluated whether RT timing impacts clinical outcomes and symptom burden in prostate cancer patients. Patients/Methods: This retrospective study (n = 336, median follow-up 55 months) included men who received curative intent external beam RT between 2010 and 2019 (median age 69, 69% black, median PSA 11.3, 40% with Gleason 8–10). Treatment times (TTs) were averaged and analyzed by quartile/median. Outcomes included freedom from biochemical failure (FFBF) and distant metastasis (FFDM), GI and GU toxicity, and quality of life (QOL). Subgroup analyses by race and hormone therapy status were performed. Results: Across the overall cohort, TT was not associated with FFBF or FFDM. However, in white men, earlier TTs were significantly associated with higher 5-year FFBF (89% vs. 67%, p = 0.0139) and FFDM (93% vs. 72%, p = 0.0268). In the multivariate analysis (MVA), TT was not associated with FFBF or FFDM for all men, but in white men, earlier TT was associated with improved FFBF (HR 2.8, p = 0.06) in a model also including risk category (p = 0.21). Overall, no significant differences were observed for grade 2–3+ toxicity and TT. Trends for inferior QOL, and worse grade 2+ (p = 0.2) and 3+ GU toxicity (p = 0.1) were observed for later TTs. In white men, bowel, urinary continence, and irritative/obstructive urinary QOL were worse with later TTs (p < 0.05). Conclusions: TT may influence clinical outcomes and symptom burden, particularly in white men. These findings underscore the potential of chronoradiotherapy as a personalized treatment strategy and highlight the need for prospective trials. Full article

Background Objectives: Parental anxiety and depression demonstrate bidirectional connections with child developmental outcomes (e.g., disruptive behavior). Directly targeting child development through behavioral parent training (BPT) has potential for reversing this cycle. Parent–Child Interaction Therapy (PCIT), a BPT with robust research evidence for decreasing child disruptive behaviors, has demonstrated promise in also decreasing caregiver anxiety and depression. However, the mechanisms that explain this relationship are less understood. Methods: The current study examined whether caregivers (N = 840) completing time-limited PCIT experienced significant reductions in depression and anxiety symptoms and improvements in child disruptive behaviors at each time point. Generalized estimate equation analyses assessed whether caregiver anxiety and depression moderated changes in child disruptive behavior. Mediation analyses explored the extent that changes in caregiver–child interactions over time explained changes in family outcomes. Results: Child disruptive behavior and caregiver depression and anxiety symptoms improved significantly at each time point of PCIT. Change in child behavioral outcomes was significantly moderated by caregiver race. Caregivers with higher anxiety reported fewer improvements in child disruptive behavior compared to other caregivers. Changes in caregiver anxiety and depression over the course of treatment were partially mediated by improvement in caregiver–child interaction skills. Changes in child disruptive behavior were not mediated by improvement in caregiver–child interaction skills. Conclusions: Results demonstrate that time-limited PCIT could significantly improve caregiver anxiety and depression, and some PCIT-taught parenting skills are direct drivers of this process. Further research is needed to understand other mechanisms underlying the relationship between PCIT and improved family outcomes. Full article

Autosomal trisomy, a genetic disorder characterized by the presence of an extra autosome, is a rare but important chromosomal abnormality in horses, often associated with infertility, developmental abnormalities, and reduced life expectancy. This study represents the largest population-level screening for autosomal trisomy in horses; the analysis used single nucleotide polymorphism (SNP) panel genotype intensity data from 17,078 horses, 6601 of which were juveniles (i.e., ≤12 months of age) when genotyped. Using methodologies adapted from similar screening studies in cattle, the only aneuploidy detected was trisomy 27 in two juvenile male Irish Sport Horses (ISH) (0.03% prevalence among juveniles or 0.01% prevalence in the overall population). One ISH colt was cytogenetically confirmed and displayed no overt external phenotypic abnormalities, while cytogenetics was not undertaken on the other ISH colt, nor was it phenotypically assessed. Parentage analysis revealed that one ISH colt inherited two different copies of chr27 from the sire, demonstrating heterodisomy, likely due to a nondisjunction event during meiosis I in the sire. The other ISH colt inherited two different copies of chr27 from the dam, also indicating heterodisomy; the dam was 23 years of age when the colt was born. Based on the observed prevalence of autosomal trisomy, it can be estimated that at least 3 foals per 10,000 live births are likely to have autosomal trisomy. Though, given that only 74 (i.e., 0.004%) of horses were genotyped within a month of birth, this is likely an underestimate. The economic consequence of undiagnosed trisomy in high-value breeding horses that are potentially infertile could be substantial. As horse genotyping for parentage verification and discovery is transitioning to medium-density single nucleotide polymorphism panels, routine genomic screening for autosomal aneuploidy could be readily undertaken and potentially should form a standard screening prerequisite along with other genetic defects at horse sales. Currently, thoroughbred horses registered for racing are not genotyped, and only a limited number of sport horse studbooks are using SNP genotyping. This highlights an opportunity for those already genotyping to expand their support for breeders through low-cost, high-value chromosomal screening at the time of registration rather than incurring additional costs over the horse’s life cycle to determine the root cause of certain phenotypes owing to the undiagnosed trisomy. Full article

Triathlon is a multi-sport event that combines swimming, cycling and running. The distances vary and the physiological demands are high. Objectives: This review compiles information on nutritional strategies and ergogenic supplements for triathlon training, competition and recovery. It aims to provide an understanding of the specific challenges and needs of the sport to help triathletes and coaches optimise performance through effective training and nutrition plans. Methods: English-language publications were searched using the keywords triathlon, nutrition, recovery and ergogenic aids, alone or in combination, in databases. Results: Maintaining good glycogen levels, consuming enough carbohydrates and staying properly hydrated are key to athletic performance, especially for triathletes. Education regarding nutrition, the role of probiotics and supplements, and diet modification for the enhancement of performance and recovery are pivotal considerations. Conclusions: Triathletes are at risk of RED-S due to negative energy balance and high fibre/plant protein diets, especially women. Optimising muscle glycogen through tailored diet and training, especially pre- and in-race nutrition, including carbohydrate loading and hydration strategies, is critical. Education is needed to improve post-exercise nutrition, while probiotics and certain supplements may aid performance and recovery. Dietary support is important for resistance training to optimise performance and recovery. Full article

Background: Birnaviruses infect a wide range of aquatic and terrestrial hosts, including several economically important fish species. This study aimed to isolate and characterize a novel birnavirus strain from mandarin fish (Siniperca chuatsi), a high-value freshwater species in Chinese aquaculture. Methods: A novel strain, designated mandarin fish birnavirus (MFBV), was isolated from diseased fish and propagated in SCK cells. The complete genome was determined using high-throughput sequencing and RACE. Viral replication kinetics, tissue distribution, and pathogenicity were assessed through in vitro infection, RT-qPCR, histopathology, and experimental challenges. In addition, disinfectant sensitivity and environmental stability were evaluated. Results: The MFBV genome comprises two segments (A: 3539 bp; B: 2719 bp), and phylogenetic analysis revealed close relatedness to largemouth bass birnavirus (LBBV) and Lates calcarifer birnavirus (LCBV). MFBV displayed rapid replication in SCK cells, completing a replication cycle in 8–10 h. In juvenile and fry fish, an experimental infection caused acute disease with cumulative mortality ranging from 41.8% to 83.6%, with fry showing higher susceptibility. Viral RNA was detected in multiple tissues (7.9 × 10⁶–7.9 × 10⁷ copies/μg RNA), and histopathological lesions were observed in the intestine, spleen, and kidney. MFBV was highly sensitive to glutaraldehyde (20 ppm), while other disinfectants showed reduced efficacy. Viral half-life ranged from 36.5 to 144.5 h at room temperature. Conclusions: These findings demonstrate that MFBV can induce acute systemic infection in mandarin fish. The results offer new insights into the genomic and biological features of birnaviruses, contributing to improved disease management and viral taxonomy. Full article

Background: Individual nutrients are associated with cognitive performance, but there is less evidence on the relationship between overall dietary patterns and cognitive performance in midlife. Objective: To examine the relation between dietary patterns and cognitive performance in midlife adults within the Bogalusa Heart Study (BHS). Methods: Cross-sectional data from the 2013–2016 cycle of the Bogalusa Heart Study, a life-course prospective cohort study, were used to generate diet quality scores, including the Alternate Healthy Eating Index 2010 (AHEI), the Healthy Eating Index 2015 (HEI), and the Alternate Mediterranean Dietary Pattern (aMed), based on food frequency questionnaires. Cognitive scores assessing attention and processing, episodic memory, and executive function were generated through validated cognitive tests. Generalized linear and logistic regression models were fit with adjustment for potential confounders. Results: Of 1053 participants included in the analysis, the mean age was 48.18 (SD = 5.22) years; 38.94% were male; and 31.14% identified as Black. Higher diet quality scores were associated with a higher global cognitive score (P-trend = 0.01 for AHEI, 0.02 for HEI, and 0.04 for aMed) after adjusting for age, sex, race, employment, education, smoking status, total energy intake, physical activity, BMI, diabetes, and hypertension. In dichotomous outcome analyses, higher AHEI and aMed scores remained inversely associated with low cognition when adjusting for similar covariates (P-trend = 0.03 for AHEI, 0.03 for aMed), but the association was attenuated to non-significance for HEI. In joint effect analysis between employment and dietary patterns (P-interaction = 0.28 for AHEI, 0.01 for HEI, and 0.11 for aMed), unemployment with a lower quality diet was associated with lower cognitive performance (P-trend = 0.02 for AHEI, 0.02 for HEI, and 0.01 for aMed). Conclusions: A high-quality diet is associated with optimal cognitive performance among midlife adults, and unemployment status may influence this association. These findings suggest that dietary interventions during midlife may represent a public health strategy to preserve cognitive function and reduce future neurodegenerative disease burden, especially in disadvantaged populations. Full article

This study aimed to examine how physiological determinants are associated with skiing technique and race performance in cross-country roller skiing by integrating motion data obtained via a Global Navigation Satellite System (GNSS) with laboratory-based physiological assessments. Nineteen well-trained male skiers completed a 10 km roller ski race, during which skiing velocity, cycle length, cycle time, and sub-technique usage were measured using GNSS. Whole-body and upper-body endurance and power were evaluated on the treadmill and ski ergometer. Time to exhaustion during the double poling test (r = −0.84, p < 0.01) and VO_2max from the pole walk and run test (r = −0.72, p < 0.01) were the strongest predictors of race performance, and both were significantly associated with skiing velocity (VO_2max: r = 0.79, p < 0.01; TTE-DPT: r = 0.81, p < 0.01) and cycle length (VO_2max: r = 0.58, p < 0.01; TTE-DPT: r = 0.47, p < 0.05) in the most frequently used technique. These findings suggest that the development of both whole-body and upper-body endurance plays a crucial role in improving technical efficiency and race performances. Full article