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16 pages, 2899 KiB  
Article
The Coupling Mechanism of the Electricity–Gas System and Assessment of Attack Resistance Based on Interdependent Networks
by Qingyu Zou and Lin Yan
Eng 2025, 6(8), 193; https://doi.org/10.3390/eng6080193 - 6 Aug 2025
Abstract
Natural gas plays a critical role in integrated energy systems. In this context, the present study proposes an optimization model for the electricity–gas coupling system, grounded in the theory of interdependent networks. By integrating network topology parameters with real-time operational metrics, the model [...] Read more.
Natural gas plays a critical role in integrated energy systems. In this context, the present study proposes an optimization model for the electricity–gas coupling system, grounded in the theory of interdependent networks. By integrating network topology parameters with real-time operational metrics, the model substantially enhances system robustness and adaptability. To quantify nodal vulnerability and importance, the study introduces two novel evaluation indicators: the Electric Potential–Closeness Fusion Indicator (EPFI) for power networks and the Pressure Difference–Closeness Comprehensive Indicator (PDCI) for natural gas systems. Leveraging these indicators, three coupling paradigms—assortative, disassortative, and random—are systematically constructed and analyzed. System resilience is assessed through simulation experiments incorporating three attack strategies: degree-based, betweenness centrality-based, and random node removal. Evaluation metrics include network efficiency and the variation in the size of the largest connected subgraph under different coupling configurations. The proposed framework is validated using a hybrid case study that combines the IEEE 118-node electricity network with a 20-node Belgian natural gas system, operating under a unidirectional gas-to-electricity energy flow model. Results confirm that the disassortative coupling configuration, based on EPFI and PDCI indicators, exhibits superior resistance to network perturbations, thereby affirming the effectiveness of the model in improving the robustness of integrated energy systems. Full article
22 pages, 6784 KiB  
Article
A Second-Order LADRC-Based Control Strategy for Quadrotor UAVs Using a Modified Crayfish Optimization Algorithm and Fuzzy Logic
by Kelin Li, Guangzhao Wang and Yalei Bai
Electronics 2025, 14(15), 3124; https://doi.org/10.3390/electronics14153124 - 5 Aug 2025
Abstract
To enhance the rapid and stable tracking of a specified trajectory by quadcopter drones, while ensuring a degree of resistance to external wind disturbances, this paper proposes an integrated control strategy that combines an optimization algorithm and fuzzy control. In this system, both [...] Read more.
To enhance the rapid and stable tracking of a specified trajectory by quadcopter drones, while ensuring a degree of resistance to external wind disturbances, this paper proposes an integrated control strategy that combines an optimization algorithm and fuzzy control. In this system, both the position and attitude loops utilize second-order Linear Active Disturbance Rejection Control (LADRC) controllers, supplemented by fuzzy controllers. These controllers have been optimized using a modified crayfish optimization algorithm (MCOA), resulting in a dual-closed-loop control system. In comparisons with both the dual-closed-loop LADRC controller and the dual-closed-loop fuzzy control LADRC controller, the proposed method reduces the rise time by 52.87% in the X-channel under wind-free conditions, reduces the maximum trajectory tracking error by 86.37% under wind-disturbed conditions, and reduces the ITAE exponent by 66.2%, which demonstrates that the newly designed system delivers excellent tracking speed and accuracy along the specified trajectory. Furthermore, it remains effective even in the presence of external disturbances, it can reliably maintain the target position and the attitude angle, demonstrating strong resistance to interference and stability. Full article
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25 pages, 13557 KiB  
Article
Mechanical Properties and Energy Absorption Characteristics of a Combined Gradient BCC Lattice Structure: A Numerical Study
by Xiangheng Zhao, Xiaoqiang Wang and Yunmiao Shang
Materials 2025, 18(15), 3652; https://doi.org/10.3390/ma18153652 - 4 Aug 2025
Viewed by 133
Abstract
As a new functional graded lattice structure construction strategy, the relative density gradient strategy has a promising future due to its ease of realization in various lattice structures. This paper proposes a BCC lattice structure combining two different lattice single cells. Based on [...] Read more.
As a new functional graded lattice structure construction strategy, the relative density gradient strategy has a promising future due to its ease of realization in various lattice structures. This paper proposes a BCC lattice structure combining two different lattice single cells. Based on this, the single cells of different structures are assigned different relative density gradients, resulting in 18 combined gradient lattice structures. Based on proving the experimental feasibility of numerical simulation, the mechanical properties and energy absorption characteristics of the combined gradient lattice structure are investigated by numerical simulation. When applied to composite lattice structures, the proposed wave-like gradient design significantly improves mechanical properties. Among the various gradient strategies examined, several have achieved mechanical performance close to that of uniform lattice structures. To some extent, this approach mitigates the common drawback of gradient lattice structures—where the relative density of the weakest layer is consistently lower than the interlayer relative density of uniform lattice structures—resulting in varying degrees of mechanical performance degradation compared to their uniform counterparts. The proposed linearly enhanced gradient strategy (Strategy-LE) possesses higher SEA and CLE values when the lattice structure is subjected to compressive loading, with an improvement of 6.36% in SEA and 61.6% in CLE over the uniform structure. Through the relative density gradient design, the adaptability of the BCC lattice structure in actual complex application scenarios is greatly enhanced, and the energy-absorbing properties of the lattice structure are greatly improved. Full article
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21 pages, 5609 KiB  
Article
Carbonation and Corrosion Durability Assessment of Reinforced Concrete Beam in Heavy-Haul Railways by Multi-Physics Coupling-Based Analytical Method
by Wu-Tong Yan, Lei Yuan, Yong-Hua Su, Long-Biao Yan and Zi-Wei Song
Materials 2025, 18(15), 3622; https://doi.org/10.3390/ma18153622 - 1 Aug 2025
Viewed by 235
Abstract
The operation of heavy-haul railway trains with large loads results in significant cracking issues in reinforced concrete beams. Atmospheric carbon dioxide, oxygen, and moisture from the atmosphere penetrate into the beam interior through these cracks, accelerating the carbonation of the concrete and the [...] Read more.
The operation of heavy-haul railway trains with large loads results in significant cracking issues in reinforced concrete beams. Atmospheric carbon dioxide, oxygen, and moisture from the atmosphere penetrate into the beam interior through these cracks, accelerating the carbonation of the concrete and the corrosion of the steel bars. The rust-induced expansion of steel bars further exacerbates the cracking of the beam. The interaction between environmental factors and beam cracks leads to a rapid decline in the durability of the beam. To address this issue, a multi-physics field coupling durability assessment method was proposed, considering concrete beam cracking, concrete carbonation, and steel bar corrosion. The interaction among these three factors is achieved through sequential coupling, using crack width, carbonation passivation time, and steel bar corrosion rate as interaction parameters. Using this method, the deterioration morphology and stiffness degradation laws of 8 m reinforced concrete beams under different load conditions, including those of heavy and light trains in heavy-haul railways, are compared and assessed. The analysis reveals that within a 100-year service cycle, the maximum relative stiffness reduction for beams on the heavy train line is 20.0%, whereas for the light train line, it is only 7.4%. The degree of structural stiffness degradation is closely related to operational load levels, and beam cracking plays a critical role in this difference. Full article
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28 pages, 10224 KiB  
Article
A Vulnerability Identification Method for Distribution Networks Integrating Fuzzy Local Dimension and Topological Structure
by Kangzheng Huang, Weichuan Zhang, Yongsheng Xu, Chenkai Wu and Weibo Li
Processes 2025, 13(8), 2438; https://doi.org/10.3390/pr13082438 - 1 Aug 2025
Viewed by 216
Abstract
As the scale of shipboard power systems expands, their vulnerability becomes increasingly prominent. Identifying vulnerable points in ship power grids is essential for enhancing system stability, optimizing overall performance, and ensuring safe navigation. To address this issue, this paper proposes an algorithm based [...] Read more.
As the scale of shipboard power systems expands, their vulnerability becomes increasingly prominent. Identifying vulnerable points in ship power grids is essential for enhancing system stability, optimizing overall performance, and ensuring safe navigation. To address this issue, this paper proposes an algorithm based on fuzzy local dimension and topology (FLDT). The algorithm distinguishes contributions from nodes at different radii and within the same radius to a central node using fuzzy sets, and then derives the final importance value of each node by combining the local dimension and topology. Experimental results on nine datasets demonstrate that the FLDT algorithm outperforms degree centrality (DC), closeness centrality (CC), local dimension (LD), fuzzy local dimension (FLD), local link similarity (LLS), and mixed degree decomposition (MDD) algorithms in three metrics: network efficiency (NE), largest connected component (LCC), and monotonicity. Furthermore, in a ship power grid experiment, when 40% of the most important nodes were removed, FLDT caused a network efficiency drop of 99.78% and reduced the LCC to 2.17%, significantly outperforming traditional methods. Additional experiments under topological perturbations—including edge addition, removal, and rewiring—also show that FLDT maintains superior performance, highlighting its robustness to structural changes. This indicates that the FLDT algorithm is more effective in identifying and evaluating vulnerable points and distinguishing nodes with varying levels of importance. Full article
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9 pages, 7006 KiB  
Interesting Images
Coral Bleaching and Recovery on Urban Reefs off Jakarta, Indonesia, During the 2023–2024 Thermal Stress Event
by Tries B. Razak, Muhammad Irhas, Laura Nikita, Rindah Talitha Vida, Sera Maserati and Cut Aja Gita Alisa
Diversity 2025, 17(8), 540; https://doi.org/10.3390/d17080540 - 1 Aug 2025
Viewed by 206
Abstract
Urban coral reefs in Jakarta Bay and the Thousand Islands, Indonesia, are chronically exposed to land-based pollution and increasing thermal stress. These reefs—including the site of Indonesia’s first recorded coral bleaching event in 1983—remain highly vulnerable to climate-induced disturbances. During the fourth global [...] Read more.
Urban coral reefs in Jakarta Bay and the Thousand Islands, Indonesia, are chronically exposed to land-based pollution and increasing thermal stress. These reefs—including the site of Indonesia’s first recorded coral bleaching event in 1983—remain highly vulnerable to climate-induced disturbances. During the fourth global coral bleaching event (GCBE), we recorded selective bleaching in the region, associated with a Degree Heating Weeks (DHW) value of 4.8 °C-weeks. Surveys conducted in January 2024 across a shelf gradient at four representative islands revealed patchy bleaching, affecting various taxa at depths ranging from 3 to 13 m. A follow-up survey in May 2024, which tracked the fate of 42 tagged bleached colonies, found that 36% had fully recovered, 26% showed partial recovery, and 38% had died. Bleaching responses varied across taxa, depths, and microhabitats, often occurring in close proximity to unaffected colonies. While some corals demonstrated resilience, the overall findings underscore the continued vulnerability of urban reefs to escalating thermal stress. This highlights the urgent need for a comprehensive and coordinated national strategy—not only to monitor bleaching and assess reef responses, but also to strengthen protection measures and implement best-practice restoration. Such efforts are increasingly critical in the face of more frequent and severe bleaching events projected under future climate scenarios. Full article
(This article belongs to the Collection Interesting Images from the Sea)
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12 pages, 1752 KiB  
Article
From Myofascial Chains to the Polyconnective Network: A Novel Approach to Biomechanics and Rehabilitation Based on Graph Theory
by Daniele Della Posta, Immacolata Belviso, Jacopo Junio Valerio Branca, Ferdinando Paternostro and Carla Stecco
Life 2025, 15(8), 1200; https://doi.org/10.3390/life15081200 - 28 Jul 2025
Viewed by 449
Abstract
In recent years, the concept of the myofascial network has transformed biomechanical understanding by emphasizing the body as an integrated, multidirectional system. This study advances that paradigm by applying graph theory to model the osteo-myofascial system as an anatomical network, enabling the identification [...] Read more.
In recent years, the concept of the myofascial network has transformed biomechanical understanding by emphasizing the body as an integrated, multidirectional system. This study advances that paradigm by applying graph theory to model the osteo-myofascial system as an anatomical network, enabling the identification of topologically central nodes involved in force transmission, stability, and coordination. Using the aNETomy model and the BIOMECH 3.4 database, we constructed an undirected network of 2208 anatomical nodes and 7377 biomechanical relationships. Centrality analysis (degree, betweenness, and closeness) revealed that structures such as the sacrum and thoracolumbar fascia exhibit high connectivity and strategic importance within the network. These findings, while derived from a theoretical modeling approach, suggest that such key nodes may inform targeted treatment strategies, particularly in complex or compensatory musculoskeletal conditions. The proposed concept of a polyconnective skeleton (PCS) synthesizes the most influential anatomical hubs into a functional core of the system. This framework may support future clinical and technological applications, including integration with imaging modalities, real-time monitoring, and predictive modeling for personalized and preventive medicine. Full article
(This article belongs to the Section Medical Research)
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24 pages, 9395 KiB  
Article
Experimental Investigation of the Seismic Behavior of a Multi-Story Steel Modular Building Using Shaking Table Tests
by Xinxin Zhang, Yucong Nie, Kehao Qian, Xinyu Xie, Mengyang Zhao, Zhan Zhao and Xiang Yuan Zheng
Buildings 2025, 15(15), 2661; https://doi.org/10.3390/buildings15152661 - 28 Jul 2025
Viewed by 278
Abstract
A steel modular building is a highly prefabricated form of steel construction. It offers rapid assembly, a high degree of industrialization, and an environmentally friendly construction site. To promote the application of multi-story steel modular buildings in earthquake fortification zones, it is imperative [...] Read more.
A steel modular building is a highly prefabricated form of steel construction. It offers rapid assembly, a high degree of industrialization, and an environmentally friendly construction site. To promote the application of multi-story steel modular buildings in earthquake fortification zones, it is imperative to conduct in-depth research on their seismic behavior. In this study, a seven-story modular steel building is investigated using shaking table tests. Three seismic waves (artificial ground motion, Tohoku wave, and Tianjin wave) are selected and scaled to four intensity levels (PGA = 0.035 g, 0.1 g, 0.22 g, 0.31 g). It is found that no residual deformation of the structure is observed after tests, and its stiffness degradation ratio is 7.65%. The largest strains observed during the tests are 540 × 10−6 in beams, 1538 × 10−6 in columns, and 669 × 10−6 in joint regions, all remaining below a threshold value of 1690 × 10−6. Amplitudes and frequency characteristics of the acceleration responses are significantly affected by the characteristics of the seismic waves. However, the acceleration responses at higher floors are predominantly governed by the structure’s low-order modes (first-mode and second-mode), with the corresponding spectra containing only a single peak. When the predominant frequency of the input ground motion is close to the fundamental natural frequency of the modular steel structure, the acceleration responses will be significantly amplified. Overall, the structure demonstrates favorable seismic resistance. Full article
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15 pages, 3131 KiB  
Article
Real-Time Experiments for Decentralized Adaptive Synchronized Motion Control of a Closed-Kinematic Chain Mechanism Robot Manipulator
by Charles C. Nguyen, Tri T. Nguyen, Tu T. C. Duong, Tuan M. Nguyen, Ha T. T. Ngo and Lu Sun
Machines 2025, 13(8), 652; https://doi.org/10.3390/machines13080652 - 25 Jul 2025
Viewed by 241
Abstract
This paper presents the results of real-time experiments conducted to evaluate the performance of a developed adaptive control scheme applied to control the motion of a real closed-kinematic chain mechanism (CKCM) robot manipulator with two degrees of freedom (DOFs). The developed control scheme, [...] Read more.
This paper presents the results of real-time experiments conducted to evaluate the performance of a developed adaptive control scheme applied to control the motion of a real closed-kinematic chain mechanism (CKCM) robot manipulator with two degrees of freedom (DOFs). The developed control scheme, referred to as the decentralized adaptive synchronized control scheme (DASCS), was the result of the combination of model reference adaptive control (MRAC) based on the Lyapunov direct method and the synchronization technique. CKCM manipulators were considered in the experimental study due to their advantages over their open-kinematic chain mechanism (OKCM) manipulator counterparts, such as higher stiffness, better stability, and greater payload. The conducted computer simulation study showed that the DASCS was able to asymptotically converge tracking errors to zero, with all the active joints moving synchronously in a prescribed way. One of the important properties of the DASCS is the independence of robot manipulator dynamics, making it computationally efficient and therefore suitable for real-time applications. The present paper reports findings from experiments in which the DASCS was applied to control the above manipulator and carry out various paths. The DASCS’s performance was compared with that of a traditional adaptive control scheme, namely the SMRACS, when both schemes were applied to track the same paths. Full article
(This article belongs to the Section Automation and Control Systems)
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14 pages, 3906 KiB  
Article
An Investigation of the Process of Risk Coupling and the Main Elements of Coal-Mine Gas-Explosion Risk
by Shugang Li and Lu Gao
Fire 2025, 8(8), 294; https://doi.org/10.3390/fire8080294 - 25 Jul 2025
Viewed by 416
Abstract
This study suggests a method for analyzing the risk of methane explosions using the N-K model and Social Network Analysis (SNA) to understand how different risk factors related to coal-mine methane explosions are connected and change over time, aiming to prevent these accidents [...] Read more.
This study suggests a method for analyzing the risk of methane explosions using the N-K model and Social Network Analysis (SNA) to understand how different risk factors related to coal-mine methane explosions are connected and change over time, aiming to prevent these accidents effectively. We identified 41 secondary risk factors and four fundamental risk factors—human, equipment, environment, and management—based on the 4M accident causation theory. The SNA model was utilized to determine the main risk factors and their evolutionary routes, while the N-K model was utilized to quantify the degree of risk coupling. The findings show that the number of risk variables engaged in the methane-explosion risk system closely correlates with the number of accidents that occur and the maximum coupling level among the four elements. The primary control factors in the methane-explosion risk system are poor equipment management, broken safety monitoring and control systems, inadequate safety education and training, safety regulation violations, and poor safety production responsibility system implementation. We utilized the primary evolution paths and key elements to propose risk control approaches. A reference for ensuring safety in coal-mine operations can be found in the research findings. Full article
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20 pages, 1901 KiB  
Article
Inverse Sum Indeg Spectrum of q-Broom-like Graphs and Applications
by Fareeha Jamal, Nafaa Chbili and Muhammad Imran
Mathematics 2025, 13(15), 2346; https://doi.org/10.3390/math13152346 - 23 Jul 2025
Viewed by 132
Abstract
A graph with q(a+t) vertices is known as a q-broom-like graph KqB(a;t), which is produced by the hierarchical product of the complete graph Kq by the rooted [...] Read more.
A graph with q(a+t) vertices is known as a q-broom-like graph KqB(a;t), which is produced by the hierarchical product of the complete graph Kq by the rooted broom B(a;t), where q3,a1 and t1. A numerical quantity associated with graph structure is called a topological index. The inverse sum indeg index (shortened to ISI index) is a topological index defined as ISI(G)=vivjE(G)dvidvjdvi+dvj, where dvi is the degree of the vertex vi. In this paper, we take into consideration the ISI index for q-broom-like graphs and perform a thorough analysis of it. We find the ISI spectrum of q-broom-like graphs and derive the closed formulas for their ISI index and ISI energy. We also characterize extremal graphs and arrange them according to their ISI index and ISI energy, respectively. Further, a quantitative structure–property relationship is used to predict six physicochemical properties of sixteen alkaloid structures using ISI index and ISI energy. Both graph invariants have significant correlation values, indicating the accuracy and utility of the findings. The conclusions made in this article can help chemists and pharmacists research alkaloids’ structures for applications in industry, pharmacy, agriculture, and daily life. Full article
(This article belongs to the Special Issue Advances in Combinatorics, Discrete Mathematics and Graph Theory)
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17 pages, 8082 KiB  
Article
NPS6D100—A 6D Nanopositioning System with Sub-10 nm Performance in a Ø100 mm × 10 mm Workspace
by Steffen Hesse, Alex Huaman, Michael Katzschmann and Ludwig Herzog
Actuators 2025, 14(8), 361; https://doi.org/10.3390/act14080361 - 22 Jul 2025
Viewed by 158
Abstract
This paper presents the development of a compact nanopositioning stage with long-range capabilities and six-degree-of-freedom (DOF) closed-loop control. The system, referred to as NPS6D100, provides Ø100 mm planar and 10 mm vertical travel range while maintaining direct force transfer to the moving platform [...] Read more.
This paper presents the development of a compact nanopositioning stage with long-range capabilities and six-degree-of-freedom (DOF) closed-loop control. The system, referred to as NPS6D100, provides Ø100 mm planar and 10 mm vertical travel range while maintaining direct force transfer to the moving platform (or slider) in all DOFs. Based on an integrated planar direct drive concept, the system is enhanced by precise vertical actuation and full 6D output feedback control. The mechanical structure, drive architecture, guiding, and measurement subsystems are described in detail, along with experimental results that confirm sub-10 nm servo errors under constant setpoint operation and in synchronized multi-axis motion scenarios. With its scalable and low-disturbance design, the NPS6D100 is well suited as a nanopositioning platform for sub-10 nm applications in nanoscience and precision metrology. Full article
(This article belongs to the Special Issue Recent Developments in Precision Actuation Technologies)
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21 pages, 1188 KiB  
Article
Enhanced Array Synthesis and DOA Estimation Exploiting UAV Array with Coprime Frequencies
by Long Zhang, Weijia Cui, Nae Zheng, Song Chen and Yuxi Du
Drones 2025, 9(8), 515; https://doi.org/10.3390/drones9080515 - 22 Jul 2025
Viewed by 201
Abstract
The challenge of achieving high-precision direction-of-arrival (DOA) estimation with enhanced degrees of freedom (DOFs) under a limited number of physical array elements remains a critical issue in array signal processing. To address this limitation, this paper makes the following three key contributions: (1) [...] Read more.
The challenge of achieving high-precision direction-of-arrival (DOA) estimation with enhanced degrees of freedom (DOFs) under a limited number of physical array elements remains a critical issue in array signal processing. To address this limitation, this paper makes the following three key contributions: (1) a novel moving sparse array synthesis model incorporating time-frequency-spatial joint processing for coprime frequencies signal sources; (2) an optimized coprime frequencies-based unmanned aerial vehicle array (CF-UAVA) configuration with derived closed-form expressions for the distribution of synthesized array; and (3) two DOA estimation methods: a group sparsity-based approach universally applicable to the proposed aperture synthesis model and a joint group sparsity and virtual array interpolation tailored for the proposed CF-UAVA configuration. Comprehensive simulation results demonstrate the superior DOA estimation accuracy and increased DOFs achieved by our proposed aperture synthesis model and DOA estimation algorithms compared to conventional approaches. Full article
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21 pages, 7180 KiB  
Article
Characteristics and Expression Profiles of Identified WRKY Genes in Barley Landraces Under Cold Stress
by Yuancheng Zhou, Yiling Wang, Ting Gao, Yongli Cao, Yong Yao, Yukun Zhao and Zhen Wang
Int. J. Mol. Sci. 2025, 26(14), 6948; https://doi.org/10.3390/ijms26146948 - 19 Jul 2025
Viewed by 239
Abstract
The WRKY gene family comprises important transcription factors widely distributed in plants and plays significant roles in the growth and development, diverse (biotic and abiotic) stress responses, and various biological processes. In the current study, 96 identified HvLWRKY genes were classified into three [...] Read more.
The WRKY gene family comprises important transcription factors widely distributed in plants and plays significant roles in the growth and development, diverse (biotic and abiotic) stress responses, and various biological processes. In the current study, 96 identified HvLWRKY genes were classified into three groups and seven subgroups. Among these, 89 genes possessed the conserved domain WRKYGQK. A total of ten motifs were harbored in HvLWRKY genes with two to four introns. Fragmental duplication was suggested to be the prime force that drove the evolution of HvLWRKY genes. A high degree of collinearity was observed between barley and Triticum spelta. Cis-elements of HvLWRKYs were closely associated with abiotic stress, light response, and hormone response; however, there were differences in the numbers among groups. HvLWRKY genes, even the paralogous gene pairs, from different clades were differentially regulated under cold treatments in two landraces. HvLWRKY33, 43, 44, 57, 65, and 77 were homologous with the relative AtWRKY genes in Arabidopsis thaliana. They are suggested to regulate abiotic and pathogen resistance of two barley landraces via SA and JA pathways. Meanwhile, some genes (for example, HvLWRKY1 and HvLWRKY32) were specifically expressed in either cold-tolerant or cold-sensitive landraces. Under cold stress, different cold-responsive patterns occurred in different barley landraces. These findings provide a foundation for further studies on cold resistance in barley landraces and offer new insights for application of WRKY genes in barley breeding. Full article
(This article belongs to the Section Molecular Plant Sciences)
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22 pages, 9633 KiB  
Article
Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
by Gustavo Carvalho, Susana Guerra, María I. Martínez-Jiménez and Luis Blanco
Int. J. Mol. Sci. 2025, 26(14), 6947; https://doi.org/10.3390/ijms26146947 - 19 Jul 2025
Viewed by 309
Abstract
The human PrimPol counteracts DNA replication stress by repriming DNA synthesis when fork progression is hindered by UV light or hydroxyurea treatment, or by encountering complex DNA structures, such as G-quadruplexes, R-loops, or interstrand crosslinks. The Mus musculus PrimPol (MmPrimPol) shares [...] Read more.
The human PrimPol counteracts DNA replication stress by repriming DNA synthesis when fork progression is hindered by UV light or hydroxyurea treatment, or by encountering complex DNA structures, such as G-quadruplexes, R-loops, or interstrand crosslinks. The Mus musculus PrimPol (MmPrimPol) shares a high degree of amino acid similarity with its human ortholog; however, as shown here, MmPrimPol exhibits a more powerful primase activity compared to the human enzyme. Such a robust primase activity relies on an enhanced ability to bind the 5′ site nucleotide, and consequently to form initial dimers and further mature primers. Additionally, a shorter linker between the AEP core and the Zn finger domain (ZnFD) in the murine homolog likely promotes a constitutive closing of these domains into a primase-ready configuration. Consequently, a reinforced close configuration of the ZnFD would explain why MmPrimPol has a more robust primase, but a very limited DNA polymerization on an existing primer. Full article
(This article belongs to the Section Molecular Genetics and Genomics)
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