Search Results (421)

Pulses and pseudocereals are sustainable protein sources of bioactive peptides (BAPs) with potential antioxidant, antihypertensive, antidiabetic, antimicrobial, and immunomodulatory activities. BAPs are typically liberated during gastrointestinal digestion or through bio-based processes, among which enzymatic hydrolysis and microbial fermentation represent the most widely applied strategies. Enzymatic hydrolysis provides controlled and reproducible release of short peptide motifs; recent advances such as ultrasound- or high-pressure–assisted hydrolysis enhance yield and bioactivity. Fermentation exploits microbial proteolytic activity to generate complex peptide mixtures, while improving sensory quality, reducing antinutritional compounds, and responding to consumer demand for natural and “clean-label” products. In silico tools increasingly complement these approaches by accelerating peptide discovery, predicting interactions with molecular targets, and guiding process design. This review provides an updated overview of bio-based methods to produce BAPs from pulses and pseudocereals, emphasizing the comparative advantages of enzymatic and fermentation technologies and their integration with computational tools. Moreover, it examines regulatory frameworks in the European Union, the United States, Japan, and China, while discussing current challenges for industrial scale-up and application in functional foods and nutraceuticals. These combined strategies offer a promising pathway to unlock the health and sustainability potential of plant proteins. Full article

Clean-label preservation of beef patties remains challenging due to rapid oxidative and microbiological spoilage during refrigeration. Incorporating Salicornia extract yielded clear, dose-dependent quality gains over 15 days at 4 ± 1 °C. Relative to the control, 1.5% Salicornia lowered secondary lipid oxidation by 42% (TBARS: 1.15 vs. 1.98 mg MDA/kg) and primary oxidation by 33% (PV: 3.30 vs. 4.95 meq O₂/kg), while maintaining a substantially higher antioxidant status (TPC: 20.6 vs. 6.8 mg GAE/100 g; DPPH: 45.8% vs. 14.5%). Microbiological loads were attenuated (SPC: 4.88 vs. 6.20 log CFU/g; psychrotrophs: 1.46 vs. 2.00 log CFU/g; yeasts/molds: 1.44 vs. 1.74 log CFU/g), accompanied by moderated physicochemical drift (pH: 6.16 vs. 5.86; a_w: 0.847 vs. 0.828). Color retention was markedly improved, with higher redness and lower overall discoloration (a*: 13.6 vs. 9.8; ΔE*: 3.96 vs. 9.13). The 1.0% treatment showed intermediate benefits, indicating a robust dose response. Collectively, these outcomes demonstrate that 1.0–1.5% Salicornia delivers multifaceted protection, limiting lipid oxidation, curbing microbial growth, preserving color, and stabilizing matrix attributes, thereby extending the refrigerated shelf-life of beef patties and supporting clean-label reformulation, particularly when combined with oxygen-limiting packaging. Full article

To address the dual challenges of fossil fuel depletion and environmental pollution, developing clean, renewable alternative fuels is an urgent need. Biomass gas, including biomass syngas and biogas, offers significant potential as an internal combustion engine alternative fuel due to its widespread availability and carbon-neutral properties. This review summarizes research on biomass gas application in dual-fuel diesel engines. Firstly, biosyngas and biogas production methods, characteristics, and purification needs are detailed, highlighting gas composition variability as a key factor impacting engine performance. Secondly, dual-fuel diesel engine operating modes and their integration with advanced low-temperature combustion technologies are analyzed. The review focuses on how biomass gas affects combustion characteristics, engine performance, and emissions. Results indicate dual-fuel mode effectively reduces diesel consumption, emissions, while its carbon-neutrality lowers life-cycle CO₂ emissions and generally suppresses NO_x formation. However, challenges include potential BTE reduction and increased CO and HC emissions at low loads. Future research should prioritize gas quality standardization, intelligent combustion system optimization, and full-chain techno-economic evaluation to advance this technology. Overall, this review concludes that dual-fuel operation with biomass gases can achieve high diesel substitution rates, significantly reducing NO_x and particulate matter emissions. However, challenges such as decreased brake thermal efficiency and increased CO and HC emissions under low-load conditions remain. Future efforts should focus on gas composition standardization, intelligent combustion control, and system-level optimization. Full article

Titanium dioxide (TiO₂) is widely used in coatings, plastics, rubber, papermaking, and other industries. The microstructural characteristics of its inorganic shell largely determine the overall performance of the product, significantly affecting optical behavior, dispersibility, weather resistance, and stability. Currently, coating quality evaluation in industry still relies primarily on manual inspection, lacking objective, standardized, and reproducible quantitative methods. This study focuses on lab-prepared core–shell TiO₂ powders comprising a TiO₂ core and a thin inorganic shell enriched in alumina/silica. This study presents Titanium Dioxide U-Net (TD U-Net)—a deep learning approach for transmission electron microscopy (TEM) image segmentation and shell thickness evaluation of core–shell structured TiO₂ particles. TD U-Net employs an encoder–decoder architecture that effectively integrates multi-scale features, addressing challenges such as blurred boundaries and low contrast. We constructed a dataset of 1479 TEM images processed through a six-step workflow: image collection, data cleaning, annotation, mask generation, augmentation, and cropping. Results show that TD U-Net achieves a Dice coefficient of 0.967 for segmentation accuracy and controls shell-thickness measurement error within 5%, significantly outperforming existing image-processing models. An intelligent analysis system developed from this technology has been successfully applied to titanium dioxide product quality assessment, providing an efficient and reliable automated tool for coating-process optimization and quality control. Full article

This study evaluated the effects of enzymatically produced collagen hydrolysate and Salicornia perennans extract on the quality, oxidative stability, and nutritional composition of canned canine meat pâtés. Two formulations were prepared: a control 2% NaCl, no hydrolysate and an experimental sample containing 3% collagen hydrolysate sheep:camel:bovine = 1:1:1, 1% Salicornia perennans extract, and 1% NaCl. Physicochemical, textural, amino-acid, fatty-acid, and oxidative parameters were monitored over 10 days of storage. The treated pâtés showed similar proximate composition moisture 76.1%, protein 9.2%, metabolizable energy (ME) 102 kcal·100 g⁻¹; p > 0.05 but exhibited enhanced functional stability, with reduced water loss syneresis 1.8 vs. 3.1%; p < 0.05 and improved cohesiveness 0.46 vs. 0.41; p < 0.05. Amino-acid enrichment included higher aspartic acid +33%; p < 0.05, methionine +53%; p < 0.05, and tryptophan +39%; p < 0.05, while the lipid profile showed lower SFA 52.8 vs. 56.4%; p < 0.05, higher n-3 PUFA 1.5 vs. 0.8%; p < 0.05, and a reduced n-6:n-3 ratio 3.8 vs. 5.6; p < 0.05. During storage, oxidative markers decreased: TBARS −45%, carbonyls −14%, acid value −18%, and color stability improved by +2.0 pp. These findings confirm the synergistic antioxidant and structuring effects of collagen-derived peptides and Salicornia polyphenols, as evidenced by a 45% reduction in TBARS, 14% lower protein carbonyls, and 18% lower acid value relative to the control (p < 0.05). This synergy enabled a sodium-reduced, clean-label formulation with improved technological performance, oxidative resistance, and shelf-life stability for functional wet dog foods. In addition, it enhanced the color and visual appeal—key attributes that influence both animal palatability and the purchasing decisions of pet owners. Full article

This study investigated the effect of storage time on the quality of low-sugar apple jams partially substituted with steviol glycosides (SGs). Apple jams were prepared with 0%, 10%, 20%, 30%, and 40% sugar replacement using highly purified SGs (95.1%). The jams were evaluated immediately after production and after 3 and 6 months of storage at 22 °C in the dark. Physicochemical analyses included dry matter, total soluble solids, vitamin C, total ash, pH, titratable acidity, malic acid, and color parameters (L*, a*, b*). Sensory and microbiological assessments were also carried out. During storage, the dry matter content significantly decreased from 41.4% (control) to 35.6% (40% SGs), while titratable acidity increased from 10.69° to 16.73° (p < 0.05), and pH values remained stable (3.15–3.29). Vitamin C content decreased significantly (from 0.56 mg/100 g to 0.19 mg/100 g; 33–66% degradation). The color of jams became lighter with increasing SG substitution (L* increased from 17.19 to 24.73; ΔE up to 9.66) and slightly darkened after storage (ΔL ≈ −1.0). Microbiological analysis confirmed complete safety, with total colony counts < 10 CFU/g and no presence of Listeria monocytogenes or coagulase-positive Staphylococcus. Sensory evaluation by a trained panel (10 assessors, aged 34–56 years, with similar training in fruit and vegetable preserve evaluation) showed that jams with 10–30% SG substitution maintained desirable apple aroma and sweetness, whereas higher SG levels enhanced metallic odor (0.12–0.95 c.u.) and bitterness (0.2–1.9 c.u.) while slightly reducing apple flavor intensity (p < 0.05). Despite these differences, all jams remained acceptable after 6 months of storage. Overall, replacing up to 40% of sucrose with steviol glycosides provided microbiological stability, controlled color changes, and acceptable sensory quality, supporting the production of low-sugar jams in line with clean-label and sustainability trends in modern food technology. Full article

South Asia’s worsening air pollution crisis represents one of the most urgent public health and environmental challenges of the 21st century. Nearly two billion people—over one-quarter of the global population—reside in this region, where air quality levels routinely exceed World Health Organization (WHO) guidelines by factors of 10 to 15. This has translated into an unprecedented health burden, with approximately two million premature deaths annually, widespread chronic respiratory and cardiovascular disease, and rising economic losses. According to recent World Bank estimates, welfare losses amount to over 5% of regional GDP, a figure far exceeding the projected costs of coordinated mitigation. Despite this, South Asia continues to lack a binding regional framework capable of addressing its shared airshed. Existing cooperative efforts—such as the Malé Declaration on Control and Prevention of Air Pollution (1998)—have provided a useful platform for dialog and pilot monitoring, but they remain voluntary, under-resourced, and insufficient to manage the transboundary nature of the crisis. National-level programs, including India’s National Clean Air Programme (NCAP), Bangladesh’s National Air Quality Management Plan (NAQMP), and Nepal’s National Air Quality Management Action Plan (AQMAP), demonstrate domestic commitment but are constrained by fragmentation, limited financing, and lack of regional integration. This gap represents the central knowledge and governance challenge that prompted the present commentary. To address it, we propose a dual-track architecture designed to institutionalize binding regional cooperation. Track A would establish a United Nations-anchored South Asian Transboundary Air Pollution Protocol, under the auspices of the United Nations Environment Programme, the World Health Organization (WHO), and the United Nations Economic and Social Commission for Asia and the Pacific (UNESCAP). This protocol would codify legally enforceable emission standards, compliance committees, financial mechanisms, and harmonized monitoring. Track B would establish a South Asian Association for Regional Cooperation (SAARC) Prime Ministers’ Council on Air Quality (SPMCAQ) to provide political leadership, align domestic implementation, and authorize rapid responses to cross-border haze events. Lessons from the Indian Ocean Experiment, the ASEAN Agreement on Transboundary Haze Pollution, and Europe’s Convention on Long-Range Transboundary Air Pollution demonstrate that legally binding agreements combined with high-level political ownership can achieve durable reductions in pollution despite geopolitical tensions. By situating South Asia within these global precedents, the proposed framework provides a pragmatic, enforceable, and politically resilient pathway to protect health, reduce economic losses, and deliver cleaner air for nearly one-quarter of humanity. Full article

Effective carbon emission control at the provincial level is essential for advancing the high-quality development of the national economy under the “dual carbon” targets. Although Hubei Province is endowed with abundant natural resources and significant potential for sustainable growth, it still faces considerable challenges in industrial and energy restructuring. Therefore, improving carbon emission efficiency (CEE) is imperative. This study thoroughly analyzes the spatial and temporal characteristics of CEE in Hubei Province. Furthermore, the spatial Durbin model (SDM) and geographically and temporally weighted regression (GTWR) were applied to analyze the determinants of changes in CEE. The results indicate that significant disparities in CEE exist across Hubei Province, with the eastern region exhibiting the highest efficiency and the central region the lowest. The year 2016 represented a turning point, as Moran’s I increased from −0.0006 in 2016 to 0.5134 in 2017, indicating a shift in the spatial pattern of CEE from a weak and insignificant spatial autocorrelation to a strong positive spatial autocorrelation. In addition, the CEE in Hubei Province demonstrated a “siphon effect” and exhibited pronounced polarization. Based on these findings, region-specific policies are proposed. The eastern region should optimize its industrial structure and strengthen urban governance. The western region should leverage its clean energy advantage and enhance carbon sink capacity. The central region should advance low-carbon industrial transformation and coordinated governance to prevent core cities from transferring resources and pollution to surrounding areas. Full article

With the increasing age and traffic load of highway networks in China, preventive maintenance has become a critical strategy for extending pavement service life and improving infrastructure sustainability. However, the lack of standardized post-evaluation systems has hindered the scientific assessment of maintenance effectiveness. This study proposes a systematic post-evaluation framework for highway preventive maintenance projects based on the Hazard Analysis and Critical Control Points (HACCP)-Inspired methodology (Applying Principles of Hazard Analysis and CCP Identification). Adopting a full life-cycle perspective, the framework identifies critical control points (CCPs) across pre-, mid-, and post-implementation phases, targeting six key dimensions: ecological and environmental hazards, resource utilization hazard, engineering safety risks, engineering quality risks, socioeconomic benefit hazards, and social living environment hazards. A multi-level evaluation indicator system is constructed using hierarchical clustering and weighted through the Analytic Hierarchy Process (AHP). The framework is applied to a preventive maintenance project on the Jinghuan Expressway in Tianjin, China, demonstrating strong practical applicability. The final evaluation score of 84.1 out of 100 confirms the technical adequacy of the project while revealing areas for improvement in clean energy adoption and substructure monitoring. This framework provides a robust basis for standardizing post-evaluation practices and promoting sustainable highway maintenance management. Full article

This study systematically compares thermal power direct carbon emission data quality control systems in China, the EU, and the U.S., quantifying differences and proposing optimization strategies. Core results: (1) Regulatory frameworks: The EU’s “Directive-Regulation-Standard” system controls data uncertainty ≤ ±3%; the U.S. “Clean Air Act+40 CFR Parts 75/98” framework achieves Continuous Emission Monitoring System (CEMS) median accuracy ~2% but has regional gaps; China’s 2024 legalization of CEMS data leaves a 32% eastern–western installation gap (~90% vs.~58%). (2) Technical paths: The EU mandates CEMS for units > 20 MW (65% ultrasonic flowmeters); the U.S. uses CEMS for coal-fired (error ≤ 1%) and accounting for gas-fired units; China’s 70% S-type Pitot tubes have 17% error in complex flow fields, while pilot multi-channel ultrasonic flowmeters reach ±1.5%. (3) Mechanisms: The EU’s QAL1-QAL3+AST cuts uncertainty by 40–60%; the U.S. NIST calibration limits cross-plant deviation ≤ 1.5%; China’s big data boosts anomaly identification by 72% but lacks full-process control. (4) China-specific proposals: Mandate CEMS for units > 300 MW, build 3–5 national flue gas platforms by 2026, and offer 60% western equipment subsidies, supporting carbon data quality improvement and international mutual recognition. Full article

Food spoilage and contamination remain pressing global challenges, undermining food security and safety while driving economic losses. Conventional preservation strategies, including thermal treatments, refrigeration, and synthetic additives, often compromise nutritional quality and raise sustainability concerns, thereby necessitating natural, effective alternatives. Curcumin, a polyphenolic compound derived from Curcuma longa, has demonstrated broad-spectrum antimicrobial, antioxidant, and anti-inflammatory activities, making it a promising candidate for food preservation. However, its poor solubility, instability, and low bioavailability limit direct applications in food systems. Advances in nanotechnology have enabled the development of nanoformulated curcumin, enhancing solubility, stability, controlled release, and functional efficacy. This review examines the antimicrobial mechanisms of curcumin and its nanoformulations, including membrane disruption, oxidative stress via reactive oxygen species, quorum sensing inhibition, and biofilm suppression. Applications in active and smart packaging are highlighted, where curcumin nanoformulation not only extends shelf life but also enables freshness monitoring through pH-responsive color changes. Evidence across meats, seafood, fruits, dairy, and beverages shows improved microbial safety, oxidative stability, and sensory quality. Multifunctional systems, such as hybrid composites and stimuli-responsive carriers, represent next-generation tools for sustainable packaging. However, challenges remain with scale-up, migration safety, cytotoxicity, and potential promotion of antimicrobial resistance gene (ARG) transfer. Future research should focus on safety validation, advanced nanocarriers, ARG-aware strategies, and regulatory frameworks. Overall, nanoformulated curcumin offers a natural, versatile, and eco-friendly approach to food preservation that aligns with clean-label consumer demand. Full article

Metal components that undergo ultrasonic cleaning are often stored in rinse water before drying; during this dwell period, surface corrosion can nucleate and grow. Here, we investigate how two easily monitored water-quality parameters—chloride ion concentration (Cl⁻) and chemical oxygen demand (COD), a proxy for residual organic species—govern the initiation and propagation of corrosion on low-carbon steel. After ultrasonic cleaning in five representative cleaning solutions, test coupons were immersed for up to 72 h in the corresponding rinse water and the extent of corrosion was quantified by optical profilometry and mass loss. The surface area covered by corrosion scaled linearly with [Cl⁻] (0–150 mg L⁻¹) and COD (5–120 mg L⁻¹), with correlation coefficients of 0.92 and 0.88, respectively. When both parameters exceeded threshold values of 50 mg L⁻¹ (Cl⁻) and 30 mg L⁻¹ (COD), the corrosion rate doubled relative to the control. A two-step mitigation strategy—ion-exchange pretreatment followed by activated-carbon polishing—reduced Cl⁻ and COD below the thresholds and suppressed corrosion formation by >70%. These findings provide a simple water-quality guideline and a low-cost process retrofit for manufacturers that store steel parts in high-humidity environments. Full article

Consumers increasingly seek clean-label meat products with improved nutrition and stability. We evaluated a collagen hydrolysate–cranberry mixture (CH-CR) as a functional additive in cooked sausages. Two formulations—control and CH-CR—were assessed for fatty acid profile; lipid and protein oxidation during storage; antioxidant capacity ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and half-maximal inhibitory concentration (IC50); amino acid composition; and instrumental color. Relative to the control, CH-CR produced a more favorable lipid profile: lower saturated fatty acids (SFAs) 23.9% vs. 28.0%, higher monounsaturated fatty acids (MUFAs) 53.2% vs. 49.3%, slightly higher polyunsaturated fatty acids (PUFAs) 23.3% vs. 22.7%, a higher PUFA/SFA ratio of 0.97 vs. 0.81, and a lower omega-6/omega-3 (n-6/n-3) ratio of 13.5 vs. 27.1, driven by higher alpha-linolenic acid (ALA) 1.6% vs. 0.8%, with trans fats <0.1%. Storage studies showed attenuated oxidation in CH-CR: lower peroxide value (PV) at day 10 8.1 ± 0.4 vs. 9.8 ± 0.5 meq/kg and lower thiobarbituric acid-reactive substances (TBARS) at day 6 0.042 ± 0.004 vs. 0.055 ± 0.006 mg MDA/kg and day 10 0.156 ± 0.016 vs. 0.590 ± 0.041 mg MDA/kg); the acid value at day 10 was similar. Antioxidant capacity increased with CH-CR FRAP 30.5 mg gallic acid equivalents (GAE)/g vs. not detected; DPPH inhibition was 29.88% vs. 10.23%; IC50 56.22 vs. 149.51 µg/mL. The amino acid profile reflected collagen incorporation—higher glycine+proline+hydroxyproline 2.37 vs. 1.38 g/100 g and a modest rise in indispensable amino acids (IAAs) 5.72 vs. 5.42 g/100 g, increasing the IAA/total amino acid (TAA) ratio to 0.411 vs. 0.380. CH-CR samples were lighter and retained redness better under light, with comparable overall color stability. Overall, CH-CR is a natural strategy to improve fatty acid quality and oxidative/color stability in sausages. Full article

The daily milk yield (DMY) is defined as the sum of milk produced during morning, midday, and evening milkings. This metric is the key parameter for quantifying a cow’s absolute production level and serves as the foundation for calculating economic traits such as 305-day total milk yield, fat yield, and protein yield, making it essential for evaluating lactation performance. Based on the data cleaning pipeline, 2992 valid records were retained from 3332 initial records through rigorous quality-control screening, this study systematically evaluated three critical lactation traits—daily milk yield and its milk components (milk fat percentage and milk protein percentage)—using complete lactation records from Huaxi cows and estimated their phenotypic and genetic parameters. Non-genetic factors (parity, season, and mature body weight) were corrected via the MIXED procedure coupled with a multi-trait animal model. The results showed that all considered non-genetic factors significantly influenced the lactation traits (p < 0.05), while daily milk yield (DMY), milk fat percentage (FP), and milk protein percentage (PP) all exhibited moderate to high heritability (0.29–0.38) (p < 0.01). Genetic evaluation of DMY and its milk components provides quantitative evidence for precision selection and optimized mating decisions in the Huaxi dairy population, thereby accelerating genetic progress in milk production, improving herd profitability, and promoting the development of the regional dairy industry. Full article

Oxidative reactions accelerate quality loss in emulsified meats. This study evaluated a clean-label strategy in goat meat pates by co-fortifying Portulaca oleracea powder 1% and honey 4%. Control and treatment batches were cooked to 72 °C and stored as opened packs at ≤6 °C for 10 days. Oxidative stability of lipid and protein was monitored by peroxide value (PV), TBARS, acid value, and baseline protein carbonyls; total antioxidant capacity was assessed by FRAP and DPPH; color was quantified in CIE Lab; fatty acids were profiled by GC-FID; and protein integrity was examined by SDS-PAGE. The treatment modestly increased α-linolenic acid (ALA) (1.2% vs. 0.8%) in the control and markedly enhanced antioxidant status (FRAP 10.5 ± 0.04 mg GAE/g vs. not detected; DPPH 33.02 ± 0.009% vs. 22.33 ± 0.007%; IC₅₀ 106.10 ± 10.01 vs. 138.25 ± 11.15 µg/mL). Across storage, PV showed a small, non-significant delay on day 10 (13.0 ± 0.9 vs. 14.0 ± 0.9 meq/kg), while secondary and hydrolytic indices were consistently lower (TBARS day 10: 1.91 ± 0.13 vs. 3.29 ± 0.23 mg MDA/kg; acid value day 10: 7.0 ± 0.5 vs. 8.5 ± 0.6 mg KOH/g). Protein carbonyls at baseline were comparable (99.19 vs. 95.73 nmol/mg). L* and b* remained similar before and after light exposure, with a modest, non-significant reduction in color stability and greater a* loss in the treatment. These results show that purslane–honey co-fortification nutritionally enriches pates and attenuates oxidative spoilage during refrigerated storage, with minor color trade-offs that merit process optimization. Full article