Search Results (20)

Extensive studies have established that ultrasonic micro-jets and acoustic cavitation selectively intensify interfacial interactions at multiphase boundaries, thereby enhancing the flotation of soluble salt minerals and oxide ores. Although a growing body of evidence shows that pulp-borne nanoparticles (i.e., nanosolids, colloids, and nanoscale gas nuclei) mediate these effects, their role in the flotation of ultrafine smithsonite after collector addition has not yet been systematically examined. To fill this gap, we compared the flotation response of ultrafine smithsonite under conventional stirring (SC) and ultrasonic conditioning (UC), using sodium oleate (NaOL) as the collector, and dissected the governing mechanisms across three pillars, mineral–NaOL interaction, particle aggregation, and frothability, with particular attention paid to how nanoparticles modulate each dimension. The flotation results show that flotation performance under UC is dictated by NaOL concentration. At low NaOL levels (i.e., below 4 × 10⁻⁴ M), UC depresses both recovery and kinetics relative to SC, while at high NaOL levels, the trend reverses and UC outperforms SC. Mechanistic analysis reveals that sonication erodes mineral surfaces and generates cavitation, flooding the pulp with various nanoparticles. When NaOL is scarce, zinc-containing components and zinc-rich nanosolids sequester the collector through non-selective adsorption and precipitation, leaving smithsonite poorly hydrophobized. Consequently, particle aggregation and pulp frothability are markedly inferior to those in the SC system, so the flotation recovery and kinetics remain lower. As the NaOL concentration rises, smithsonite becomes adequately hydrophobized, and the pulp fills with hydrophobic zinc-rich nanosolids, along with cavitation-induced gas nuclei or tiny bubbles. These nanoparticles now act as bridges, accelerating the aggregation of ultrafine smithsonite once sonication stops and agitation begins, while simultaneously improving frothability. Although the strong dispersive action of ultrasound still suppresses initial flotation kinetics, cumulative recovery ultimately surpasses that of SC. The findings delineate a nanoparticle-regulated flotation paradigm and establish a critical NaOL concentration window for effective UC in ultrafine smithsonite flotation. This framework is readily transferable to the beneficiation of other ultrafine, soluble oxidized minerals (rhodochrosite, dolomite, etc.). Full article

Objective: In previous studies, echogenic liposomes with liquid and gas cores were analyzed as alternative carriers of drug molecules and cavitation nuclei for sonoporation. The possibility of small interfering RNA (si-RNA) encapsulation has also been presented. In this study, the usability of echogenic liposomes as drug carriers and cavitation seeds was evaluated using an in vivo model. Methods: A doxorubicin-loaded echogenic liposome was synthesized as a drug carrier. The size distribution and the number of formed echogenic liposomes were measured. Five comparative in vivo experiments were conducted with and without doxorubicin-loaded echogenic liposomes, and the results were statically analyzed. Results: Sonoporation with doxorubicin-loaded echogenic liposomes at 3.05 W/cm² of ISPTA ultrasound sonication and 0.98 MHz results in an average tumor volume growth of less than 25% of that following the simple administration of doxorubicin. Considering the p-value between the two groups is approximately 0.03, doxorubicin-loaded echogenic liposomes were effectively applicable as cavitation nuclei for sonoporation. Conclusions: Although further studies are needed to clarify the responses to incident ultrasound fields, the proposed echogenic liposome appears to be a promising alternative cavitation nuclei/carrier for sonoporation. Full article

Microbubbles, acting as cavitation nuclei, undergo cycles of expansion, contraction, and collapse. This collapse generates shockwaves, alters local shear forces, and increases local temperature. Cavitation causes severe changes in pressure and temperature, resulting in surface erosion. Shockwaves strip material from surfaces, forming pits and cracks. Prolonged cavitation reduces the mechanical strength and fatigue life of materials, potentially leading to failure. Controlling bubble size and generating monodispersed bubbles is crucial for accurately modeling cavitation phenomena. In this work, we generate monodispersed microbubbles with controllable size using a novel and low-cost microfluidic method. We created an innovative T-junction structure that controls the two-phase flow for tiny, monodispersed bubble generation. Monodisperse microbubbles with diameters below one-fifth of the channel width (W = 100 µm) are produced due to the controlled pressure gradient. This microstructure, fabricated by a CNC milling technique, produces 20 μm bubbles without requiring high-resolution equipment and cleanroom environments. Bubble size is controlled with gas and liquid pressure ratio and microgeometry. This microbubble generation method provides a controllable and reproducible way for cavitation research. Full article

Background: Pharmacologically targeting the STING pathway offers a novel approach to cancer immunotherapy. However, small-molecule STING agonists face challenges such as poor tumor accumulation, rapid clearance, and short-lived effects within the tumor microenvironment, thus limiting their therapeutic potential. To address the challenges of poor specificity and inadequate targeting of STING in breast cancer treatment, herein, we report the design and development of a targeted liposomal delivery system modified with the tumor-targeting peptide iRGD (iRGD-STING-PFP@liposomes). With LIFU irradiation, the liposomal system exploits acoustic cavitation, where gas nuclei form and collapse within the hydrophobic region of the liposome lipid bilayer (transient pore formation), which leads to significantly enhanced drug release. Methods: Transmission electron microscopy (TEM) was used to investigate the physicochemical properties of the targeted liposomes. Encapsulation efficiency and in vitro release were assessed using the dialysis bag method, while the effects of iRGD on liposome targeting were evaluated through laser confocal microscopy. The CCK-8 assay was used to investigate the toxicity and cell growth effects of this system on 4T1 breast cancer cells and HUVEC vascular endothelial cells. A subcutaneous breast cancer tumor model was established to evaluate the tumor-killing effects and therapeutic mechanism of the newly developed liposomes. Results: The liposome carrier exhibited a regular morphology, with a particle size of 232.16 ± 19.82 nm, as indicated by dynamic light scattering (DLS), and demonstrated low toxicity to both HUVEC and 4T1 cells. With an encapsulation efficiency of 41.82 ± 5.67%, the carrier exhibited a slow release pattern in vitro after STING loading. Targeting results indicated that iRGD modification enhanced the system’s ability to target 4T1 cells. The iRGD-STING-PFP@liposomes group demonstrated significant tumor growth inhibition in the subcutaneous breast cancer mouse model with effective activation of the immune system, resulting in the highest populations of matured dendritic cells (71.2 ± 5.4%), increased presentation of tumor-related antigens, promoted CD8+ T cell infiltration at the tumor site, and enhanced NK cell activity. Conclusions: The iRGD-STING-PFP@liposomes targeted drug delivery system effectively targets breast cancer cells, providing a new strategy for breast cancer immunotherapy. These findings indicate that iRGD-STING-PFP@liposomes could successfully deliver STING agonists to tumor tissue, trigger the innate immune response, and may serve as a potential platform for targeted immunotherapy. Full article

Due to the scale effect, tip vortex cavitation (TVC) is the earliest type of cavitation that occurs on real ship propellers. As a result, experts in the ship field have been paying close attention to the accurate prediction of propeller TVC inception for a long time. The motion and growth of the microscopic nuclei in the water have a significant influence on TVC inception. However, the minimum pressure coefficient method—a common method at present—based on the traditional Eulerian framework, neglects the influence of microscopic nuclei and therefore cannot accurately predict the cavitation inception. Moreover, the numerical prediction method for cavitation inception, which is based on bubble dynamics models and considers the influence of nuclei, has not established a set of unified and specific discrimination criteria applicable to propeller cavitation inception. In order to make up for the shortcomings of traditional prediction models and the existing methods based on bubble dynamics in the prediction of TVC inception, we propose a new discrimination method for propeller TVC inception based on bubble dynamics in this paper. The comparison with experimental results demonstrates that our proposed method allows us to predict propeller TVC inception more accurately. In addition, the effect mechanism of tip vortex flow characteristics on nuclei evolution is further investigated, and it is found that when approaching the low-pressure region at a vortex core under the influence of tip vortex suction, nuclei grow explosively under the continuous action of the low pressure at the vortex core until they reach their maximum sizes and then collapse rapidly. Full article

Ultrasound-mediated cavitation shows great promise for improving targeted drug delivery across a range of clinical applications. Cavitation nuclei—sound-sensitive constructs that enhance cavitation activity at lower pressures—have become a powerful adjuvant to ultrasound-based treatments, and more recently emerged as a drug delivery vehicle in their own right. The unique combination of physical, biological, and chemical effects that occur around these structures, as well as their varied compositions and morphologies, make cavitation nuclei an attractive platform for creating delivery systems tuned to particular therapeutics. In this review, we describe the structure and function of cavitation nuclei, approaches to their functionalization and customization, various clinical applications, progress toward real-world translation, and future directions for the field. Full article

The acoustic cavitation of fluids, as well as related physical and chemical phenomena, causes a variety of effects that are highly important in technological processes and medicine. Therefore, it is important to be able to control the conditions that allow cavitation to begin and progress. However, the accurate prediction of acoustic cavitation is dependent on a complex relationship between external influence parameters and fluid characteristics. The multiparameter problem restricts the development of successful theoretical models. As a result, it is critical to identify the most important parameters influencing the onset of the cavitation process. In this paper, the ultrasonic frequency, hydrostatic pressure, temperature, degassing, density, viscosity, volume, and surface tension of a fluid were investigated using machine learning to determine their significance in predicting acoustic cavitation strength. Three machine learning models based on support vector regression (SVR), ridge regression (RR), and random forest (RF) algorithms with different input parameters were trained. The results showed that the SVM algorithm performed better than the other two algorithms. The parameters affecting the active cavitation nuclei, namely hydrostatic pressure, ultrasound frequency, and outgassing degree, were found to be the most important input parameters influencing the prediction of the cavitation threshold. Other parameters have a minor impact when compared to the first three, and their role can be compensated for by alternative variables. The further development of the obtained results provides a new way to optimize and improve existing theoretical models. Full article

Pancreatic tumors can be resistant to drug penetration due to high interstitial fluid pressure, dense stroma, and disarrayed vasculature. Ultrasound-induced cavitation is an emerging technology that may overcome many of these limitations. Low-intensity ultrasound, coupled with co-administered cavitation nuclei consisting of gas-stabilizing sub-micron scale SonoTran Particles, is effective at increasing therapeutic antibody delivery to xenograft flank tumors in mouse models. Here, we sought to evaluate the effectiveness of this approach in situ using a large animal model that mimics human pancreatic cancer patients. Immunocompromised pigs were surgically engrafted with human Panc-1 pancreatic ductal adenocarcinoma (PDAC) tumors in targeted regions of the pancreas. These tumors were found to recapitulate many features of human PDAC tumors. Animals were intravenously injected with the common cancer therapeutics Cetuximab, gemcitabine, and paclitaxel, followed by infusion with SonoTran Particles. Select tumors in each animal were targeted with focused ultrasound to induce cavitation. Cavitation increased the intra-tumor concentrations of Cetuximab, gemcitabine, and paclitaxel by 477%, 148%, and 193%, respectively, compared to tumors that were not targeted with ultrasound in the same animals. Together, these data show that ultrasound-mediated cavitation, when delivered in combination with gas-entrapping particles, improves therapeutic delivery in pancreatic tumors under clinically relevant conditions. Full article

The presence of multi-drug resistant biofilms in chronic, persistent infections is a major barrier to successful clinical outcomes of therapy. The production of an extracellular matrix is a characteristic of the biofilm phenotype, intrinsically linked to antimicrobial tolerance. The heterogeneity of the extracellular matrix makes it highly dynamic, with substantial differences in composition between biofilms, even in the same species. This variability poses a major challenge in targeting drug delivery systems to biofilms, as there are few elements both suitably conserved and widely expressed across multiple species. However, the presence of extracellular DNA within the extracellular matrix is ubiquitous across species, which alongside bacterial cell components, gives the biofilm its net negative charge. This research aims to develop a means of targeting biofilms to enhance drug delivery by developing a cationic gas-filled microbubble that non-selectively targets the negatively charged biofilm. Cationic and uncharged microbubbles loaded with different gases were formulated and tested to determine their stability, ability to bind to negatively charged artificial substrates, binding strength, and, subsequently, their ability to adhere to biofilms. It was shown that compared to their uncharged counterparts, cationic microbubbles facilitated a significant increase in the number of microbubbles that could both bind and sustain their interaction with biofilms. This work is the first to demonstrate the utility of charged microbubbles for the non-selective targeting of bacterial biofilms, which could be used to significantly enhance stimuli-mediated drug delivery to the bacterial biofilm. Full article

Interfacial nanobubbles on a superhydrophobic surface can serve as ultrasound cavitation nuclei for continuously promoting sonodynamic therapy, but their poor dispersibility in blood has limited their biomedical application. In this study, we proposed ultrasound-responsive biomimetic superhydrophobic mesoporous silica nanoparticles, modified with red blood cell membrane and loaded with doxorubicin (DOX) (F-MSN-DOX@RBC), for RM-1 tumor sonodynamic therapy. Their mean size and zeta potentials were 232 ± 78.8 nm and −35.57 ± 0.74 mV, respectively. The F-MSN-DOX@RBC accumulation in a tumor was significantly higher than in the control group, and the spleen uptake of F-MSN-DOX@RBC was significantly reduced in comparison to that of the F-MSN-DOX group. Moreover, the cavitation caused by a single dose of F-MSN-DOX@RBC combined with multiple ultrasounds provided continuous sonodynamic therapy. The tumor inhibition rates in the experimental group were 71.5 8 ± 9.54%, which is significantly better than the control group. DHE and CD31 fluorescence staining was used to assess the reactive oxygen species (ROS) generated and the broken tumor vascular system induced by ultrasound. Finally, we can conclude that the combination of anti-vascular therapy, sonodynamic therapy by ROS, and chemotherapy promoted tumor treatment efficacy. The use of red blood cell membrane-modified superhydrophobic silica nanoparticles is a promising strategy in designing ultrasound-responsive nanoparticles to promote drug-release. Full article

Infection with SARS-CoV-2, the virus responsible for the global COVID-19 pandemic, causes a respiratory illness that can severely impact other organ systems and is possibly precipitated by cytokine storm, septic shock, thrombosis, and oxidative stress. SARS-CoV-2 infected individuals may be asymptomatic or may experience mild, moderate, or severe symptoms with or without pneumonia. The mechanisms by which SARS-CoV-2 infects humans are largely unknown. Mouse hepatitis virus 1 (MHV-1)-induced infection was used as a highly relevant surrogate animal model for this study. We further characterized this animal model and compared it with SARS-CoV-2 infection in humans. MHV-1 inoculated mice displayed death as well as weight loss, as reported earlier. We showed that MHV-1-infected mice at days 7–8 exhibit severe lung inflammation, peribronchiolar interstitial infiltration, bronchiolar epithelial cell necrosis and intra-alveolar necrotic debris, alveolar exudation (surrounding alveolar walls have capillaries that are dilated and filled with red blood cells), mononuclear cell infiltration, hyaline membrane formation, the presence of hemosiderin-laden macrophages, and interstitial edema. When compared to uninfected mice, the infected mice showed severe liver vascular congestion, luminal thrombosis of portal and sinusoidal vessels, hepatocyte degeneration, cell necrosis, and hemorrhagic changes. Proximal and distal tubular necrosis, hemorrhage in interstitial tissue, and the vacuolation of renal tubules were observed. The heart showed severe interstitial edema, vascular congestion, and dilation, as well as red blood cell extravasation into the interstitium. Upon examination of the MHV-1 infected mice brain, we observed congested blood vessels, perivascular cavitation, cortical pericellular halos, vacuolation of neuropils, darkly stained nuclei, pyknotic nuclei, and associated vacuolation of the neuropil in the cortex, as well as acute eosinophilic necrosis and necrotic neurons with fragmented nuclei and vacuolation in the hippocampus. Our findings suggest that the widespread thrombotic events observed in the surrogate animal model for SARS-CoV-2 mimic the reported findings in SARS-CoV-2 infected humans, representing a highly relevant and safe animal model for the study of the pathophysiologic mechanisms of SARS-CoV-2 for potential therapeutic interventions. Full article

Cavitation phenomenon is important in hydraulic turbomachineries. With the construction of pumping stations and hydro power stations on plateau, the influence of nuclei fraction on cavitation becomes important. As a simplified model, a twisted hydrofoil was used in this study to understand the cavitation behaviors on pump impeller blade and turbine runner blade at different altitude levels. The altitudes of 0 m, 1000 m, 2000 m, 3000 m and 4000 m were comparatively studied for simulating the plateau situation. Results show that the cavitation volume proportion f_cav increases with the decreasing of cavitation coefficient C_σ. At a specific C_σ, high altitude and few nuclei will cause smaller size of cavitation. The smaller C_σ is, the higher the sensitivity Δf_cav is. The larger C_σ is, the higher the relative sensitivity Δf_cav^* is. On the twisted foil, flow incidence angle increases from the sidewall to mid-span with the decreasing of the local minimum pressure. When C_σ is continually decreasing, the size of cavitation extends in spanwise, streamwise and thickness directions. The cavity is broken by the backward-jet flow when C_σ becomes small. A tail generates and the cavity becomes relatively unstable. This study will provide reference for evaluating the cavitation status of the water pumps and hydroturbines installed on a plateau with high altitude level. Full article

Hydraulic torque converter is widely used in transmission units as it is able to provide variable speed and torque ratio, isolate vibration, and absorb shock. The pursuit of a highly packed power unit requires a high capacity/speed torque converter, consequently resulting in a higher risk for cavitation and severe performance degradation, noise, vibration, and even failure. Existing cavitation models generally focus on water, and the empirical parameters are not suitable for the cavitation prediction of torque converter which utilizes high viscosity oil as its working medium. This paper focused on the influence of parameters on the performance and cavitation characteristics of torque converter. A full flow passage geometry and different computational fluid dynamics (CFD) models with cavitation were developed to predict torque converter fluid behavior by resolving Reynolds-averaged Navier–Stokes equations using finite volume method (FVM). The numerical results indicated that nuclei volume fraction, vaporization coefficient, mean nucleation site radius, and maximum density ratio have great influences on the cavitation behavior. These parameters altered the degree of cavitation and the pressure distribution on the surface of stator blades, and affected the stall performance such as stall capacity factor and torque ratio. The cavitation model was then modified to improve calculation accuracy. The test results showed that the prediction error under stall operating condition was decreased from 6.7% to 2%. This study provides insight on the influences of the empirical parameters on both internal cavitation behavior as well as overall hydrodynamic performance. Full article

Researchers are still working on the development of models that facilitate the accurate estimation of acoustic cavitation threshold. In this paper, we have analyzed the possibility of using the incubation time criterion to calculate the threshold of the onset of acoustic cavitation depending on the ultrasound frequency, hydrostatic pressure, and temperature of a liquid. This criterion has been successfully used by earlier studies to calculate the dynamic strength of solids and has recently been proposed in an adapted version for calculating the cavitation threshold. The analysis is carried out for various experimental data for water presented in the literature. Although the criterion assumes the use of macroparameters of a liquid, we also considered the possibility of taking into account the size of cavitation nuclei and its influence on the calculation result. We compared the results of cavitation threshold calculations done using the incubation time criterion of cavitation and the classical nucleation theory. Our results showed that the incubation time criterion more qualitatively models the results of experiments using only three parameters of the liquid. We then discussed a possible relationship between the parameters of the two approaches. The results of our study showed that the criterion under consideration has a good potential and can be conveniently used for applications where there are special requirements for ultrasound parameters, maximum negative pressure, and liquid temperature. Full article

In this study, the high-fidelity numerical methods are developed to investigate the tip vortex cavitation (TVC) inception and noise of underwater propellers, namely, Model-A and Model-B, which are designed to investigate the effects of sweep angle on cavitation inception and noise. In addition, the entire body of the DARPA Suboff submarine is included to consider the effects of the inflow distortion originating from the boundary layer flow of the submarine body on the cavitating flow of the propellers. The Eulerian approach consisting of Reynolds-averaged Navier–Stokes (RANS) solver and the vortex model is coupled with the Lagrangian approach using the bubble dynamics equations and the acoustic analogy for nuclei initially distributed in inlet flow. First, three-dimensional incompressible unsteady RANS simulations are performed to predict the hydrodynamic flow field driven by underwater propellers installed on a DARPA Suboff submarine body. The Scully vortex model and dissipation vortex model (DVM) are used to regenerate the tip vortex dissipated by artificial numerical damping and low grid resolution around the vortex core center, which is identified by using minimum λ₂-criterion in the swirling flow field originating from the propeller blade tip. Then, tip vortex cavitation inception is simulated by applying the bubble dynamics equations to nuclei initially distributed in the inflow region. The volume and location of each nucleus are obtained by solving the bubble dynamics equations on the flow field obtained using the Eulerian method. Finally, the cavitation noise is predicted by modeling each bubble with a point monopole source whose strength is proportional to its volume acceleration. The validity of the present numerical methods is confirmed by comparing the predicted acoustic pressure spectrum with the measured ones. Full article