Search Results (3)

Prokaryotic communities and physico-chemical characteristics of 30 brine samples from the thalassohaline Tuz Lake (Salt Lake), Deep Zone, Kayacik, Kaldirim, and Yavsan salterns (Turkey) were analyzed using 16S rRNA amplicon sequencing and standard methods, respectively. Archaea (98.41% of reads) was found to dominate in these habitats in contrast to the domain Bacteria (1.38% of reads). Representatives of the phylum Euryarchaeota were detected as the most predominant, while 59.48% and 1.32% of reads, respectively, were assigned to 18 archaeal genera, 19 bacterial genera, 10 archaeal genera, and one bacterial genus that were determined to be present, with more than 1% sequences in the samples. They were the archaeal genera Haloquadratum, Haloarcula, Halorhabdus, Natronomonas, Halosimplex, Halomicrobium, Halorubrum, Halonotius, Halolamina, Halobacterium, and Salinibacter within the domain Bacteria. The genera Haloquadratum and Halorhabdus were found in all sampling sites. While Haloquadratum, Haloarcula, and Halorhabdus were the most abundant genera, two uncultured Tuz Lake Halobacteria (TLHs) 1 and 2 were detected in high abundance, and an additional uncultured haloarchaeal TLH-3 was found as a minor abundant uncultured taxon. Their future isolation in pure culture would permit us to expand our knowledge on hypersaline thalassohaline habitats, as well as their ecological role and biomedical and biotechnological potential applications. Full article

As one of the largest hypersaline lakes, Lake Tuz, located in the middle of Turkey, is a key waterbird habitat and is classified as a Special Environmental Protection Area in the country. It is a dynamic lake, highly affected by evaporation due to its wide expanse and shallowness (water depth <40 cm), in addition to being externally exploited by salt companies. Monitoring the dynamics of its changes in volume, which cause ecological problems, is required to protect its saline lake functions. In this context, a spatially homogeneous distributed gauge could be critical for monitoring and rapid response; however, the number of gauge stations and their vicinity is insufficient for the entire lake. The present study focuses on assessing the feasibility of a time-series interferometric technique, namely the small baseline subset (SBAS), for monitoring volume dynamics, based on freely available Sentinel-1 data. A levelling observation was also performed to quantify the accuracy of the SBAS results. Regression analysis between water levels, which is one of the most important volume dynamics, derived by SBAS and levelling in February, April, July and October was 67%, 80%, 84%, and 95% respectively, for correlation in the range of 10–40 cm in water level, and was in line with levelling. Salt lake components such as water, vegetation, moist soil, dry soil, and salt, were also classified with Sentinel-2 multispectral images over time to understand the reliability of the SBAS measurements based on interferometric coherence over different surface types. The findings indicate that the SBAS method with Sentinel-1 is a good alternative for measuring lake volume dynamics, including the monitoring of water level and salt movement, especially for the dry season. Even though the number of coherent, measurable, samples (excluding water) decrease during the wet season, there are always sufficient coherent samples (>0.45) over the lake. Full article

The indiscriminate use of chemical pesticides alongside the expansion of large-scale industries globally can critically jeopardize marine ecology and the well-being of mankind. This is because the agricultural runoffs and industrial effluents eventually enter waterways before flowing into highly saline environments i.e., oceans. Herein, the study assessed two novel bacterial isolates, Bacillus subtilis strain H1 and Bacillus thuringiensis strain H2 from the hypersaline Lake Tuz in Turkey to degrade recalcitrant haloalkanoic acids, haloacetates and chlorpyrifos, and consequently, identify their optimal pollutant concentrations, pH and temperature alongside salt-tolerance thresholds. Bacillus strains H1 and H2 optimally degraded 2,2-dichloropropionic acid (2,2-DCP) under similar incubation conditions (pH 8.0, 30 °C), except the latter preferred a higher concentration of pollutants as well as salinity at 30 mM and 35%, respectively, while strain H1 grew well on 20 mM at <30%. While both isolates could degrade all substrates used, the dehalogenase gene from strain H1 could not be amplified. Capacity of the H2 bacterial isolate to degrade 2,2-DCP was affirmed by the detection of the 795 bp putative halotolerant dehalogenase gene after a successful polymerase chain reaction (PCR) amplification. Hence, the findings envisage the potential of both isolates as bio-degraders of recalcitrant halogenated compounds and those of the same chemical family as chlorpyrifos, in saline environments. Full article