Search Results (10)

Canola (Brassica napus L.) is an important oilseed crop that provides essential vegetable oil but faces significant competition from weeds that are influenced by various agronomic practices and environmental conditions. This study examines the complex interactions between canola stand density and weed intensity over three growing seasons, identifying a total of 27 weed species. It is important to establish a connection between the density of winter canola stands, the intensity of weeding and the response of individual weed species in real conditions. The case study was executed on plots located in the Přerov district (Olomouc region, Czech Republic). The assessment was carried out during two periods—autumn in October and spring in April. Canola plants (plant density) were counted in each evaluated area, weed species were identified, and the number of plants for each weed species was determined. Half of the plots were covered with foil before herbicide application to prevent these areas from being treated with herbicides. We used redundancy analysis (RDA) to evaluate the relationships between canola density and weed dynamics, both with and without herbicide treatment. The results show the ability of canola to compete with weeds; however, that is factored by the density of the canola stand. In dense stands (over 60 plants/m²), canola is able to suppress Galium aparine L., Geranium pusillum L., Lamium purpureum L., Papaver rhoeas L. and Chamomilla suaveolens (Pursh) Rydb. Nevertheless, there are weed species that grow well even in dense canola stands (Echinochloa crus-galli (L.) P. Beauv., Phragmites australis (Cav.) Steud., Tripleurospermum inodorum (L.) Sch. Bip. and Triticum aestivum L.). These findings highlight the potential for using canola stand density as a strategic component of integrated weed management to reduce herbicide reliance and address the growing challenge of herbicide-resistant weed populations. This research contributes significantly to our understanding of the dynamics of weed competition in canola systems and informs sustainable agricultural practices for improved crop yield and environmental stewardship. Full article

Rapeseed, weeds, and pathogens interact with each other. However, these interactions are not well understood. The aim of our work was to describe the relationships between weed vegetation and pathogen manifestations in rapeseed stands. Results from the four seasons show that different rapeseed stand structures produce different weed and pathogen responses. Eighteen weed species were identified in the rapeseed stands. The selected characteristics of rapeseed stands, pathogens, and weed manifestations were evaluated using redundancy analysis. Rapeseed stands with the highest levels of pathogens present (Alternaria brassiceae (Berk.) Sacc., Botrytis cinerea (De Bary) Whetzel, Sclerotinia sclerotiorum (Lib.) de Bary, Verticilium longisporum (C.Stark) Karapapa, Bainbr & Heale) had the lowest seed yield. There, the weeds Cirsium arvense (L.) Scop., Tripleurospermum inodorum (L.) Sch. Bip., Sonchus arvensis L. were more abundant in dense stands, and Phoma lingam (telomorph: Leptosphaeria maculans Ces. & De Not.) was more common. Mutual positive interactions may also include the relationship between weed species of the Asteraceae family and increased manifestations of Phoma lingam. A similar relationship can be expected for the weeds Capsella bursa-pastoris (L.) Medik., Descurainia sophia (L.) Prantl and Sclerotinia sclerotiorum symptoms. Full article

Weed infestation of cereal crops in organic farming is becoming a serious problem in agriculture. Sustainable agriculture requires the search for and implementation of crop management techniques that will reduce weeds without negatively impacting the environment. This research refers to the principles of integrated plant protection in sustainable agriculture, allowing the use of chemical plant protection products to be limited to the absolute minimum. Technology for growing spring barley based on the use of bacterial consortia in combination with living mulch (LM) can be an interesting approach to this problem. The aim of this three-year field research was to determine the effects of bacterial consortia and LM on the level of weed infestation in the organic spring barley crop. Two factors were tested in the experiment: bacterial consortia factors: control (without bacterial consortia); 1—Bacillus megaterium var. phosphaticum and Arthrobacter agilis; 2—Bacillus subtilis, Bacillus amyloliquefaciens, and Pseudomonas fluorescens; and LM: control (without LM); red clover; red clover + Italian ryegrass; and Italian ryegrass. This research demonstrated that the bacterial consortia tested significantly reduced both the biomass and number of weeds, including the following dominant weeds: Chenopodium album, Sinapis arvensis, Elymus repens, and Tripleurospermum inodorum. The use of LM also significantly reduced the weed infestation of spring barley stands. The lowest biomass and number of weeds, with the exception of Elymus repens, were recorded on objects with LM Italian ryegrass in spring barley in combination with bacterial consortium 2. The introduction of cultivation with LM Italian ryegrass or its mixture with red clover and the use of bacteria should be recommended for the practice of sustainable agriculture, which will reduce weeds through an ecological method. Full article

The development of new drugs derived from plant sources is of significant interest in modern pharmacy. One of the promising plant sources for introduction into pharmaceuticals is Tripleurospermum inodorum (L.) Sch. Bip., also known as Tripleurospermum perforatum (Merat.) M. This plant has been shown to possess various biological activities, including anti-inflammatory, antimicrobial, and antimycotic activities, among others. However, a review of the current literature reveals a paucity of studies investigating the chemical composition of the herb Tripleurospermum inodorum (L.) Sch. Bip. This study presents the development of a method for obtaining an extract of the herb Tripleurospermum inodorum (L.) Sch. Bip. enriched with flavonoids, harvested before flowering and butonization. This study focused on determining the optimal conditions for extraction, including the concentration of the extractant (ethanol), extraction time, raw material/extractant ratio, extraction frequency, complexation reaction time, amount of aluminum chloride solution, and amount of diluted acetic acid. The results indicate that herbs harvested during this specific period exhibited a higher flavonoid content compared to those collected during butonization and flowering. Moreover, this study demonstrated that the flavonoid content could exceed 7% mg REq/100 g D.W. through a one-hour extraction process. Furthermore, the flavonoid content was found to be 7.65 ± 0.03 mg REq/100 g D.W. following a three-minute ultrasound-assisted extraction process, followed by thermal extraction. A qualitative analysis identified a variety of phenolic compounds in the extract, such as chlorogenic acid, 5-O-p-coumaroylquinic acid, 1-O-p-coumaroylquinic acid, luteolin-7-glucoside, quercetin-3-glucoside, luteolin-7-rutinoside, 3,5-O-dicaffeoylquinic acid, quercetin-3-O-malonylglucoside, apigenin-7-glucoside, luteolin-3-malonylglucoside, cynarin, rhamnetin-3-(O-dimethyl rhamnosyl glucosylglucoside), and luteolin. Moreover, this study demonstrated the antimicrobial, anti-inflammatory, anticoagulant, anti-aggregation, and antioxidant activities of the aqueous alcoholic extract from T. inodorum herb (ETIH) against pathogens such as Staphylococcus aureus, Escherichia coli, and Candida albicans. Additionally, the extract exhibited comparable anti-inflammatory effects on diclofenac sodium. These findings contribute to the understanding of the potential pharmacological applications of the developed herb extract. Full article

This review provides a comprehensive overview of the botany, traditional uses, phytochemistry, pharmacology, and toxicity of the genus Tripleurospermum. Tripleurospermum, a prominent genus within the family Asteraceae, is recognized for its therapeutic potential in treating various ailments, including skin, digestive, and respiratory diseases; cancer; muscular pain; and stress and as a sedative. Through extensive phytochemical studies regarding the Tripleurospermum species, numerous chemical compounds have been identified and classified into distinct classes, predominantly encompassing terpenes, hydrocarbons, steroids, hydrocarbons, oxygenated compounds, flavonoids, tannins, alcohols, acids, melatonin, and fragrant compounds. The findings from this review highlight the presence of bioactive compounds within the Tripleurospermum species that possess significant medicinal properties. Full article

The biological efficacy of herbicides MCPA+tribenuron-methyl (code name: MT-565 SG) and diflufenican+chlorotoluron (Legato Pro 425 SC) was estimated in eighteen field experiments on winter cereals in Poland and Germany to control broadleaf weeds. Postemergence application of tribenuron-methyl in combination with MCPA, applied at the 3-leaf stage to 3 tillers detectable in autumn in winter cereals, resulted in the majority of weed species occurring in autumn being effectively eliminated with MCPA+tribenuron-methyl applied at 1.0 kg∙ha^–1. It also provided an acceptable (82.4–94.1%) and comparable level of control to commonly occurring weeds Brassica napus, Capsella bursa-pastoris, Centaurea cyanus, Lamium purpureum, Tripleurospermum inodorum, Stellaria media, and Thlaspi arvense. A satisfactory level of control of 66.3 to 88.3% was confirmed for Veronica persica, Viola arvensis, and Galium aparine. According to these results, the formulation of tribenuron-methyl combined with MCPA can be recommended for application in winter cereals in the autumn as an alternative to commonly available herbicides. Full article

Turnip yellows virus (TuYV) is one of the most important pathogens of oilseed rape worldwide. The virus has a large host range including many crop species (e.g., oilseed rape, pea, chickpea) and weeds from more than twenty plant families. Other than oilseed rape, we detected TuYV in many commonly grown weed species that share the fields and vegetation period together with canola crops in Czech and Slovak Republics. TuYV was detected by reverse-transcription polymerase chain reaction (RT-PCR) in at least 26 species including main crop hosts (oilseed rape), intercrops and weeds such as Amaranthus retroflexus, Atriplex patula (Amaranthaceae), Arctium lappa, Lactuca serriola, Taraxacum officinale, Tripleurospermum inodorum (Asteraceae), Phacelia tanacetifolia (Boraginaceae), Brassica napus, Capsella bursa–pastoris, Descurainia Sophia, Raphanus raphanistrum, Sinapis alba, Sisymbrium officinale, Thlaspi arvense (Brassicaceae), Silene alba, Stellaria media (Caryophyllaceae), Euphorbia helioscopia (Euphorbiaceae), Geranium rotundifolium (Geraniaceae), Lamium purpureum (Lamiaceae), Fumaria officinalis, Papaver rhoeas (Papaveraceae), Veronica persica (Plantaginaceae syn. Scrophulariaceae), Fallopia convolvulus (Polygonaceae), Solanum nigrum (Solanaceae), Urtica dioica (Urticaceae) and Viola arvensis (Violaceae). The detection of TuYV was further confirmed by RT-qPCR as well as Sanger sequencing of the PCR fragments. We discovered four new weed species as hosts of TuYV such as T. inodorum, S. alba, G. rotundifolium and E. helioscopia, representing their three respective plant families. The readthrough domain (RTD) gene sequence analysis of the Czech and Slovak TuYV isolates from oilseed rape and weed species showed similar within-group nucleotide divergence (7.1% and 5.6%, respectively) and the absence of geographical- or host-based phylogenetic clustering. The high-throughput sequencing of the P. rhoeas sample enabled the obtention of a nearly complete genome of TuYV and revealed the mixed infection of TuYV with turnip mosaic virus and cucumber mosaic virus. Our results thus show that weed species are an important TuYV reservoir and play a significant role in the spread and incidence of the disease in field crops such as oilseed rape. Full article

The Asteraceae is a large family, rich in ornamental, economical, and medicinally valuable plants. The current study involves the analytical and pharmacological assessment of the methanolic extracts of three less investigated Asteraceae plants, namely Echinops ritro, Centaurea deflexa, and Tripleurospermum decipiens, obtained by three different extraction methodologies viz. maceration (MAC), ultrasound-assisted extraction (UAE), and homogenizer-assisted extraction (HAE). LC-MS-MS analysis of E. ritro, C. deflexa, and T. decipiens extracts led to the identification of ca. 29, 20, and 33 metabolites, respectively, belonging to flavonoids, phenolic acids, and fatty acids/amides. Although there were significant differences in the quantitative metabolite profiles in the extracts of E. ritro and T. decipiens based on the used extraction method, no significant variation was observed in the extracts of C. deflexa in the three implemented extraction techniques. The antioxidant activities of the nine extracts were assessed in vitro using six different assays viz. DPPH, ABTS, CUPRAC, FRAP, PDA, and metal chelation assay (MCA). The HAE/UAE extracts of E. ritro and the UAE/ MAC extracts of C. deflexa displayed the highest antioxidant activity in the DPPH assay, while the UAE extract of T. decipiens showed the strongest antioxidant activity in both the CUPRAC and MCA assays. The enzyme inhibitory activities of the nine extracts were studied in vitro on five different enzymes viz. tyrosinase, α-amylase, α-glucosidase, acetylcholinesterase (AChE), and butyrylcholinestrase (BChE), affecting various pathological diseases. Concerning C. deflexa, its MAC /UAE extracts showed the strongest inhibition on α-amylase, while its UAE/HAE extracts displayed strong inhibitory power on AChE. However, no significant difference was observed on their effects on tyrosinase or BChE. For T. decipiens, its UAE/HAE showed potent inhibition to α-glucosidase, MAC/ HAE significantly inhibited AChE and BChE, while UAE could strongly inhibit tyrosinase enzyme. For E. ritro, all extracts equally inhibited α-amylase and α-glucosidase, MAC/HAE strongly affected tyrosinase, HAE/MAC best inhibited BChE, while HAE inhibited AChE to a greater extent. Chemometric analysis using PCA plot was able to discriminate between the plant samples and between the implemented extraction modes. The in vitro enzyme inhibitory activities of the extracts were supported by in silico data, where metabolites, such as the lignan arctiin and the flavonoid vicenin-2, dominating the extract of C. deflexa, displayed strong binding to AChE. Similarly, chlorogenic and dicaffeoyl quinic acids, which are some of the major metabolites in the extracts of E. ritro and T. decipiens, bound with high affinity to α-glucosidase. Full article

A field experiment was carried out at the Experimental Station of Vytautas Magnus University Agriculture Academy in 2019–2020, in Lithuania. The aim of the study was to determine and to compare weed spread and caraway (Carum carvi L.) crop productivity in sole (spring barley, spring wheat, pea, caraway), binary (spring barley–caraway, spring wheat–caraway, pea–caraway) and trinary (spring barley–caraway–white clover, spring wheat–caraway–white clover, pea–caraway–white clover) crops. We hypothesized that the application of a multi-cropping system would inhibit the spread of weeds and increase the productivity of caraway crop. Objectives: (1) to determine the weed species composition in multi-cropping (sole, binary, and trinary) systems; (2) to determine the number and dry biomass of weeds in multi-cropping (sole, binary, trinary) systems; (3) to evaluate the yield of caraway seeds grown in multi-cropping systems. The dry biomass of weeds was established before harvesting the main crop (spring barley, spring wheat, and pea) (2019), and during the second year (2020) of caraway vegetative season—before harvesting caraway and spring barley in 10 randomly selected sites of 0.06 m² in each harvested plot. The number and species composition of the weeds were determined in the laboratory, and the weeds were dried in an oven at 60 °C and weighed. The number of weeds was recalculated to pcs. m⁻² and the dry biomass to g m⁻². Caraway seed yield calculation was based on a standard 12% moisture content and absolutely clean seed content (t ha⁻¹). In the first year of caraway vegetative season (2019), 23 weed species were found in multi-crops, including 19 annual weeds and 4 perennial ones. The weeds found belong to 11 different families. Two annual dicotyledonous weed species predominated: white goosefoot (Chenopodium album L.) and scentless chamomile (Tripleurospermum perforatum (Merat) M. Lainz). In the second year of the caraway vegetative season (2020), 22 weed species were found in multi-crops systems, including 18 annual weeds and 4 perennial ones. The weeds found belong to 11 different families. Scentless chamomile (Tripleurospermum perforatum (Merat) M. Lainz) and common dandelion (Taraxacum officinale F.H. Wigg.) were dominant in binary and trinary crops. Significantly higher yield of caraway seed, from 1.7 to 3.7 times, was formed when it was grown after pea, compared to other crops. Full article

We investigated if hot exhaust gas from a combine harvester could be used to reduce germination or kill weed seeds during the harvesting process. During the threshing and cleaning process in the combine, weed seeds and chaff are separated from the crop grains. After this separation, weed and crop seeds not collected can be exposed to exhaust gas before seeds are returned to the field. Seeds of some common weed species (Alopecurus myosuroides, Centaurea cyanus, Geranium pusillum, Lapsana communis, Lolium perenne, Rumex crispus, Spergula arvensis, and Tripleurospermum inodorum) were treated with exhaust gas at temperatures of 75 °C or 85 °C, 110 °C, and 140 °C for 2, 4, and 6 s, respectively. Afterwards, the seeds were germinated for 16 days. We found that 75 °C and 85 °C were insufficient to significantly reduce germination of the seeds after three durations. Some seeds were still able to germinate after 4 s exposure of 110 °C. An exposure of 140 °C for 4 and 6 s repressed germination of all species. We conclude that there is potential to develop combine harvesters that exploit the exhaust gas to either kill or reduce the ability of weed seeds to germinate before seeds are returned to the field. Full article