Search Results (2)

Bacteriophages are viruses that infect bacteria and are present in niches where bacteria thrive. In recent years, the suggested application areas of lytic bacteriophage have been expanded to include therapy, biocontrol, detection, sanitation, and remediation. However, phage application is constrained by the phage’s host range—the range of bacterial hosts sensitive to the phage and the degree of infection. Even though phage isolation and enrichment techniques are straightforward protocols, the correlation between the enrichment technique and host range profile has not been evaluated. Agar-based methods such as spotting assay and efficiency of plaquing (EOP) are the most used methods to determine the phage host range. These methods, aside from being labor intensive, can lead to subjective and incomplete results as they rely on qualitative observations of the lysis/plaques, do not reflect the lytic activity in liquid culture, and can overestimate the host range. In this study, phages against three bacterial genera were isolated using three different enrichment methods. Host range profiles of the isolated phages were quantitatively determined using a high throughput turbidimetric protocol and the data were analyzed with an accessible analytic tool “PHIDA”. Using this tool, the host ranges of 9 Listeria, 14 Salmonella, and 20 Pseudomonas phages isolated with different enrichment methods were quantitatively compared. A high variability in the host range index (HR_i) ranging from 0.86–0.63, 0.07–0.24, and 0.00–0.67 for Listeria, Salmonella, and Pseudomonas phages, respectively, was observed. Overall, no direct correlation was found between the phage host range breadth and the enrichment method in any of the three target bacterial genera. The high throughput method and analytics tool developed in this study can be easily adapted to any phage study and can provide a consensus for phage host range determination. Full article

There has been substantial urban growth in Stockholm, Sweden, the fastest-growing capital in Europe. The intensifying urbanization poses challenges for environmental management and sustainable development. Using Sentinel-2 and SPOT-5 imagery, this research investigates the evolution of land-cover change in Stockholm County between 2005 and 2015, and evaluates urban growth impact on protected green areas, green infrastructure and urban ecosystem service provision. One scene of 2015 Sentinel-2A multispectral instrument (MSI) and 10 scenes of 2005 SPOT-5 high-resolution instruments (HRI) imagery over Stockholm County are classified into 10 land-cover categories using object-based image analysis and a support vector machine algorithm with spectral, textural and geometric features. Reaching accuracies of approximately 90%, the classifications are then analyzed to determine impact of urban growth in Stockholm between 2005 and 2015, including land-cover change statistics, landscape-level urban ecosystem service provision bundle changes and evaluation of regional and local impact on legislatively protected areas as well as ecologically significant green infrastructure networks. The results indicate that urban areas increased by 15%, while non-urban land cover decreased by 4%. In terms of ecosystem services, changes in proximity of forest and low-density built-up areas were the main cause of lowered provision of temperature regulation, air purification and noise reduction. There was a decadal ecosystem service loss of 4.6 million USD (2015 exchange rate). Urban areas within a 200 m buffer zone around the Swedish environmental protection agency’s nature reserves increased 16%, with examples of urban areas constructed along nature reserve boundaries. Urban expansion overlapped the deciduous ecological corridor network and green wedge/core areas to a small but increasing degree, often in close proximity to weak but important green links in the landscape. Given these findings, increased conservation/restoration focus on the region’s green weak links is recommended. Full article