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28 pages, 13851 KiB  
Article
A Spatially Aware Machine Learning Method for Locating Electric Vehicle Charging Stations
by Yanyan Huang, Hangyi Ren, Xudong Jia, Xianyu Yu, Dong Xie, You Zou, Daoyuan Chen and Yi Yang
World Electr. Veh. J. 2025, 16(8), 445; https://doi.org/10.3390/wevj16080445 (registering DOI) - 6 Aug 2025
Abstract
The rapid adoption of electric vehicles (EVs) has driven a strong need for optimizing locations of electric vehicle charging stations (EVCSs). Previous methods for locating EVCSs rely on statistical and optimization models, but these methods have limitations in capturing complex nonlinear relationships and [...] Read more.
The rapid adoption of electric vehicles (EVs) has driven a strong need for optimizing locations of electric vehicle charging stations (EVCSs). Previous methods for locating EVCSs rely on statistical and optimization models, but these methods have limitations in capturing complex nonlinear relationships and spatial dependencies among factors influencing EVCS locations. To address this research gap and better understand the spatial impacts of urban activities on EVCS placement, this study presents a spatially aware machine learning (SAML) method that combines a multi-layer perceptron (MLP) model with a spatial loss function to optimize EVCS sites. Additionally, the method uses the Shapley additive explanation (SHAP) technique to investigate nonlinear relationships embedded in EVCS placement. Using the city of Wuhan as a case study, the SAML method reveals that parking site (PS), road density (RD), population density (PD), and commercial residential (CR) areas are key factors in determining optimal EVCS sites. The SAML model classifies these grid cells into no EVCS demand (0 EVCS), low EVCS demand (from 1 to 3 EVCSs), and high EVCS demand (4+ EVCSs) classes. The model performs well in predicting EVCS demand. Findings from ablation tests also indicate that the inclusion of spatial correlations in the model’s loss function significantly enhances the model’s performance. Additionally, results from case studies validate that the model is effective in predicting EVCSs in other metropolitan cities. Full article
(This article belongs to the Special Issue Fast-Charging Station for Electric Vehicles: Challenges and Issues)
20 pages, 2046 KiB  
Article
Satellite-Measured Suspended Particulate Matter Flux and Freshwater Flux in the Yellow Sea and East China Sea
by Wei Shi and Menghua Wang
Remote Sens. 2025, 17(15), 2726; https://doi.org/10.3390/rs17152726 (registering DOI) - 6 Aug 2025
Abstract
Traditionally, the surface suspended particulate matter (SPM) and freshwater fluxes have been computed using in situ SPM, salinity, and current measurements or through the numerical modeling. In this study, satellite-derived SPM concentration, ocean current, and sea surface salinity (SSS) are used to demonstrate [...] Read more.
Traditionally, the surface suspended particulate matter (SPM) and freshwater fluxes have been computed using in situ SPM, salinity, and current measurements or through the numerical modeling. In this study, satellite-derived SPM concentration, ocean current, and sea surface salinity (SSS) are used to demonstrate the capability to characterize and quantify the surface SPM flux and freshwater flux in the Yellow Sea (YS) and East China Sea (ECS). The different routes for SPM and freshwater to transport from the coastal region to the interior ECS are identified. The seasonal and interannual SPM and freshwater fluxes from the coastal region of the ECS are further characterized and quantified. The average SPM flux reaches ~0.3–0.4 g m−2 s−1 along the route. The SPM and the freshwater fluxes in the region show different seasonality. The intensified SPM flux from the ECS coast to the offshore in winter is one order higher than the SPM flux in summer, while the offshore freshwater flux peaks in summer and weakens significantly in winter. Particularly, we found that the SPM and SSS features in the ECS changed in response to the 2020 summer Yangtze River flood event. These spatial and temporal changes for SPM and SSS in the ECS in the 2020 summer and early autumn were attributed to the anomalous surface SPM and freshwater fluxes in the same period. Full article
(This article belongs to the Special Issue Remote Sensing for Ocean-Atmosphere Interaction Studies)
32 pages, 1845 KiB  
Article
Enhancing Smart and Zero-Carbon Cities Through a Hybrid CNN-LSTM Algorithm for Sustainable AI-Driven Solar Power Forecasting (SAI-SPF)
by Haytham Elmousalami, Felix Kin Peng Hui and Aljawharah A. Alnaser
Buildings 2025, 15(15), 2785; https://doi.org/10.3390/buildings15152785 - 6 Aug 2025
Abstract
The transition to smart, zero-carbon cities relies on advanced, sustainable energy solutions, with artificial intelligence (AI) playing a crucial role in optimizing renewable energy management. This study evaluates state-of-the-art AI models for solar power forecasting, emphasizing accuracy, reliability, and environmental sustainability. Using operational [...] Read more.
The transition to smart, zero-carbon cities relies on advanced, sustainable energy solutions, with artificial intelligence (AI) playing a crucial role in optimizing renewable energy management. This study evaluates state-of-the-art AI models for solar power forecasting, emphasizing accuracy, reliability, and environmental sustainability. Using operational data from Benban Solar Park in Egypt and Sakaka Solar Power Plant in Saudi Arabia, two of the world’s largest solar installations, the research highlights the effectiveness of hybrid AI techniques. The hybrid Convolutional Neural Network–Long Short-Term Memory (CNN-LSTM) model outperformed other models, achieving a Mean Absolute Percentage Error (MAPE) of 2.04%, Root Mean Square Error (RMSE) of 184, Mean Absolute Error (MAE) of 252, and R2 of 0.99 for Benban, and an MAPE of 2.00%, RMSE of 190, MAE of 255, and R2 of 0.98 for Sakaka. This model excels at capturing complex spatiotemporal patterns in solar data while maintaining low computational CO2 emissions, supporting sustainable AI practices. The findings demonstrate the potential of hybrid AI models to enhance the accuracy and sustainability of solar power forecasting, thereby contributing to efficient, resilient, and zero-carbon urban environments. This research provides valuable insights for policymakers and stakeholders aiming to advance smart energy infrastructure. Full article
(This article belongs to the Special Issue Intelligent Automation in Construction Management)
15 pages, 1805 KiB  
Article
Indoor Application of Coupled FLOCponics System with Caipira Lettuce (Lactuca sativa) Affects the Growth Performance and Water Characteristics of Far Eastern Catfish (Silurus asotus) and Tropical Eel (Anguilla bicolor)
by Jun Seong Park, Hae Seung Jeong, Jeong-ho Lee and Ju-ae Hwang
Animals 2025, 15(15), 2305; https://doi.org/10.3390/ani15152305 - 6 Aug 2025
Abstract
In this study, we sought to improve the productivity of Far Eastern catfish (Silurus asotus) and tropical eel (Anguilla bicolor), which are high-value fish species in the Republic of Korea, as well as that of associated crops by applying [...] Read more.
In this study, we sought to improve the productivity of Far Eastern catfish (Silurus asotus) and tropical eel (Anguilla bicolor), which are high-value fish species in the Republic of Korea, as well as that of associated crops by applying biofloc technology (BFT)-based aquaponics systems. The following three systems were used: the flow-through system (FTS), BFT, and BFT aquaponics system (BAPs). Caipira lettuce (Lactuca sativa) was utilized and hydroponics (HP) was implemented to compare crop productivity. After 42 days of treatment, the BAPs and BFT systems improved fish productivity, with weight gain rates of 134.47 ± 1.80% in BAPs-cat, 130.38 ± 0.95% in BFT, and 114.21 ± 6.62% in FTS for S. asotus, and 70.61 ± 3.26% in BAPs-eel, 62.37 ± 7.04% in BFT, and 47.83 ± 1.09% in FTS for A. bicolor. During the experiment, the total ammonia nitrogen and NO2-N concentrations were stable in all plots. In the case of NO3-N, BFT showed an increasing tendency while both BAPs showed a decrease compared with that of the BFT. BAPs-cat (total weight: 224.1 ± 6.37 g) and HP (220.3 ± 7.17 g) resulted in similar growth. However, in BAPs-eel was 187.7 ± 3.46 g due to root degradation. Water content analysis showed that BAPs-cat and BAPs-eel contained sufficient K, Ca, P, and S, which are important for crop growth. Overall, the effect of BAPs on fish growth was higher than that of FTS. This study reveals that integrating BFT with aquaponics improves productivity for high-value fish and associated crops while maintaining stable water quality. This method offers sustainable, efficient production, reduces environmental impact, and provides insights for future research in sustainable aquaculture practices. Full article
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17 pages, 1865 KiB  
Article
Biomarkers in Renal Cell Carcinoma: A Systematic Review and Immunohistochemical Validation Study
by Brett Berezowski, Robert Boothe, Billy Chaplin, Sharon J. Del Vecchio, Zakariya Fares, Tyrone L. R. Humphries, Keng Lim Ng, Taylor Noonan, Hemamali Samaratunga, Aaron Urquhart, David A. Vesey, Simon T. Wood, Glenda C. Gobe and Robert J. Ellis
Cancers 2025, 17(15), 2588; https://doi.org/10.3390/cancers17152588 - 6 Aug 2025
Abstract
Background and Objectives: The worldwide incidence of renal cell carcinoma (RCC) rose by 22% between 2012 and 2022. In Australia, RCC accounted for 2.8% of all cancer diagnoses and contributing to 1.8% of cancer-related deaths. Identification of RCC biomarkers may aid in [...] Read more.
Background and Objectives: The worldwide incidence of renal cell carcinoma (RCC) rose by 22% between 2012 and 2022. In Australia, RCC accounted for 2.8% of all cancer diagnoses and contributing to 1.8% of cancer-related deaths. Identification of RCC biomarkers may aid in diagnosis and management. Methods: A systematic review of immunohistochemical markers of RCC studies published between 1990 and 2019 was undertaken to select candidate biomarkers of RCC. Immunohistochemical staining of 73 clear cell RCC tumors and paired normal tissue was undertaken using selected markers. Semi-quantitative and quantitative analysis of staining intensity between paired samples was undertaken to evaluate utility as potential biomarkers, using Chi-square tests and paired t-tests for analysis. As an exploratory analysis, staining intensity was also compared on clinical/demographic variables using linear and logistic regression. Results: There were 123 candidate biomarkers identified in 91 studies. Four candidate markers were selected for further investigation: aminopeptidase A (APA)/cluster of differentiation (CD)249, aminopeptidase N (APN)/CD13, gamma-glutamyl transferase (GGT), and neuron-specific enolase (NSE). APA, GGT, and APN all demonstrated reduced staining intensity in the tumor compared with normal tissue (p < 0.001 for all). NSE demonstrated a statistically significant increase in expression in tumor compared with normal tissue (p < 0.001), and this was more pronounced in patients aged >60 years (p = 0.038). Conclusions: The utility of APA, APN, and GGT as diagnostic biomarkers in clear cell RCC is limited. NSE may have some role as a biomarker for clear cell RCC, particularly among older patients; however, further investigation is required. Full article
(This article belongs to the Special Issue Optimizing Surgical Procedures and Outcomes in Renal Cancer)
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11 pages, 671 KiB  
Article
Impact of Mattress Use on Sacral Interface Pressure in Community-Dwelling Older Adults
by Hye Young Lee, In Sun Jang, Jung Eun Hong, Je Hyun Kim and Seungmi Park
Geriatrics 2025, 10(4), 107; https://doi.org/10.3390/geriatrics10040107 - 6 Aug 2025
Abstract
Background/Objectives: Pressure injuries are a significant concern among older adults, particularly in community-based long-term care settings where prolonged immobility is prevalent. This study aimed to identify factors influencing sacral interface pressure in community-dwelling older adults, with an emphasis on support surface usage and [...] Read more.
Background/Objectives: Pressure injuries are a significant concern among older adults, particularly in community-based long-term care settings where prolonged immobility is prevalent. This study aimed to identify factors influencing sacral interface pressure in community-dwelling older adults, with an emphasis on support surface usage and clinical risk indicators. Methods: A total of 210 participants aged 65 years and older, all receiving long-term care services in South Korea, were enrolled in this study. Sacral interface pressure was measured in the supine position using a portable pressure mapping device (Palm Q7). General characteristics, Braden Scale scores, Huhn Scale scores, and mattress usage were assessed. Data were analyzed using descriptive statistics, t-tests, chi-square tests, and logistic regression. Results: Mattress non-use was identified as the strongest predictor of elevated sacral interface pressure (OR = 6.71, p < 0.001), followed by Braden Scale scores indicating moderate risk (OR = 4.8, p = 0.006). Huhn Scale scores were not significantly associated with interface pressure. These results suggest that support surface quality and skin condition have a stronger impact on interface pressure than mobility-related risk factors. Conclusions: The findings highlight the importance of providing high-quality pressure-relieving mattresses and implementing standardized nursing assessments to reduce the risk of pressure injuries. Integrating smart technologies and expanding access to advanced support surfaces may aid in developing tailored preventive strategies for vulnerable older adults. Full article
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15 pages, 316 KiB  
Article
Evaluation of Diet Quality, Physical Health, and Mental Health Baseline Data from a Wellness Intervention for Individuals Living in Transitional Housing
by Callie Millward, Kyle Lyman, Soonwye Lucero, James D. LeCheminant, Cindy Jenkins, Kristi Strongo, Gregory Snow, Heidi LeBlanc, Lea Palmer and Rickelle Richards
Nutrients 2025, 17(15), 2563; https://doi.org/10.3390/nu17152563 - 6 Aug 2025
Abstract
Background/Objectives: The aim of this study was to evaluate baseline health measurements among transitional housing residents (n = 29) participating in an 8-week pilot wellness intervention. Methods: Researchers measured anthropometrics, body composition, muscular strength, cardiovascular indicators, physical activity, diet quality, [...] Read more.
Background/Objectives: The aim of this study was to evaluate baseline health measurements among transitional housing residents (n = 29) participating in an 8-week pilot wellness intervention. Methods: Researchers measured anthropometrics, body composition, muscular strength, cardiovascular indicators, physical activity, diet quality, and health-related perceptions. Researchers analyzed data using descriptive statistics and conventional content analysis. Results: Most participants were male, White, and food insecure. Mean BMI (31.8 ± 8.6 kg/m2), waist-to-hip ratio (1.0 ± 0.1 males, 0.9 ± 0.1 females), body fat percentage (25.8 ± 6.1% males, 40.5 ± 9.4% females), blood pressure (131.8 ± 17.9/85.2 ± 13.3 mmHg), and daily step counts exceeded recommended levels. Absolute grip strength (77.1 ± 19.4 kg males, 53.0 ± 15.7 kg females) and perceived general health were below reference standards. The Healthy Eating Index-2020 score (39.7/100) indicated low diet quality. Common barriers to healthy eating were financial constraints (29.6%) and limited cooking/storage facilities (29.6%), as well as to exercise, physical impediments (14.8%). Conclusions: Residents living in transitional housing have less favorable body composition, diet, and grip strength measures, putting them at risk for negative health outcomes. Wellness interventions aimed at promoting improved health-related outcomes while addressing common barriers to proper diet and exercise among transitional housing residents are warranted. Full article
(This article belongs to the Special Issue Nutrition in Vulnerable Population Groups)
29 pages, 945 KiB  
Article
Modeling Based on Machine Learning and Synthetic Generated Dataset for the Needs of Multi-Criteria Decision-Making Forensics
by Aleksandar Aleksić, Radovan Radovanović, Dušan Joksimović, Milan Ranđelović, Vladimir Vuković, Slaviša Ilić and Dragan Ranđelović
Symmetry 2025, 17(8), 1254; https://doi.org/10.3390/sym17081254 - 6 Aug 2025
Abstract
Information is the primary driver of progress in today’s world, especially given the vast amounts of data available for extracting meaningful knowledge. The motivation for addressing the problem of forensic analysis—specifically the validity of decision making in multi-criteria contexts—stems from its limited coverage [...] Read more.
Information is the primary driver of progress in today’s world, especially given the vast amounts of data available for extracting meaningful knowledge. The motivation for addressing the problem of forensic analysis—specifically the validity of decision making in multi-criteria contexts—stems from its limited coverage in the existing literature. Methodologically, machine learning and ensemble models represent key trends in this domain. Datasets used for such purposes can be either real or synthetic, with synthetic data becoming particularly valuable when real data is unavailable, in line with the growing use of publicly available Internet data. The integration of these two premises forms the central challenge addressed in this paper. The proposed solution is a three-layer ensemble model: the first layer employs multi-criteria decision-making methods; the second layer implements multiple machine learning algorithms through an optimized asymmetric procedure; and the third layer applies a voting mechanism for final decision making. The model is applied and evaluated through a case study analyzing the U.S. Army’s decision to replace the Colt 1911 pistol with the Beretta 92. The results demonstrate superior performance compared to state-of-the-art models, offering a promising approach to forensic decision analysis, especially in data-scarce environments. Full article
(This article belongs to the Special Issue Symmetry or Asymmetry in Machine Learning)
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21 pages, 19752 KiB  
Article
Phase Characterisation for Recycling of Shredded Waste Printed Circuit Boards
by Laurance Donnelly, Duncan Pirrie, Matthew Power and Andrew Menzies
Recycling 2025, 10(4), 157; https://doi.org/10.3390/recycling10040157 - 6 Aug 2025
Abstract
In this study, we adopt a geometallurgical analytical approach common in mineral processing in the characterization of samples of shredded waste printed circuit board (PCB) E-waste, originating from Europe. Conventionally, bulk chemical analysis provides a value for E-waste; however, chemical analysis alone does [...] Read more.
In this study, we adopt a geometallurgical analytical approach common in mineral processing in the characterization of samples of shredded waste printed circuit board (PCB) E-waste, originating from Europe. Conventionally, bulk chemical analysis provides a value for E-waste; however, chemical analysis alone does not provide information on the textural variability, phase complexity, grain size, particle morphology, phase liberation and associations. To address this, we have integrated analysis using binocular microscopy, manual scanning electron microscopy, phase, textural and compositional analyses by automated (SEM-EDS), phase analysis based on (Automated Material Identification and Classification System (AMICS) software, and elemental analysis using micro-XRF. All methods used have strengths and limitations, but an integration of these analytical tools allows the detailed characterization of the texture and composition of the E-waste feeds, ahead of waste reprocessing. These data can then be used to aid the design of optimized processing circuits for the recovery of the key payable components, and assist in the commercial trading of e-scrap. Full article
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13 pages, 1424 KiB  
Article
Comparison of Artificial Intelligence–Derived Heart Age with Chronological Age Using Normal Sinus Electrocardiograms in Patients with No Evidence of Cardiac Disease
by Myoung Jung Kim, Sung-Hee Song, Young Jun Park, Young-Hyun Lee, Jongwoo Kim, JaeHu Jeon, KyungChang Woo, Juwon Kim, Ju Youn Kim, Seung-Jung Park, Young Keun On and Kyoung-Min Park
J. Clin. Med. 2025, 14(15), 5548; https://doi.org/10.3390/jcm14155548 - 6 Aug 2025
Abstract
Background/Objectives: Chronological age (CA) is commonly used in clinical decision-making, yet it may not accurately reflect biological aging. Recent advances in artificial intelligence (AI) allow estimation of electrocardiogram (ECG)-derived heart age, which may serve as a non-invasive biomarker for physiological aging. This [...] Read more.
Background/Objectives: Chronological age (CA) is commonly used in clinical decision-making, yet it may not accurately reflect biological aging. Recent advances in artificial intelligence (AI) allow estimation of electrocardiogram (ECG)-derived heart age, which may serve as a non-invasive biomarker for physiological aging. This study aimed to develop and validate a deep learning model to predict ECG-heart age in individuals with no structural heart disease. Methods: We trained a convolutional neural network (DenseNet-121) using 12-lead ECGs from 292,484 individuals (mean age: 51.4 ± 13.8 years; 42.3% male) without significant cardiac disease. Exclusion criteria included missing age data, age <18 or >90 years, and structural abnormalities. CA was used as the target variable. Model performance was evaluated using the coefficient of determination (R2), Pearson correlation coefficient (PCC), mean absolute error (MAE), and root mean square error (RMSE). External validation was conducted using 1191 independent ECGs. Results: The model demonstrated strong predictive performance (R2 = 0.783, PCC = 0.885, MAE = 5.023 years, RMSE = 6.389 years). ECG-heart age tended to be overestimated in younger adults (≤30 years) and underestimated in older adults (≥70 years). External validation showed consistent performance (R2 = 0.703, PCC = 0.846, MAE = 5.582 years, RMSE = 7.316 years). Conclusions: The proposed AI-based model accurately estimates ECG-heart age in individuals with structurally normal hearts. ECG-derived heart age may serve as a reliable biomarker of biological aging and support future risk stratification strategies. Full article
(This article belongs to the Section Cardiology)
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13 pages, 3237 KiB  
Article
Evaluating the Trophic Structure of an Artificial Macroalgal Bed of Eisenia bicyclis Using C and N Stable Isotopes
by Dong-Young Lee, Dongyoung Kim, Chan-Kil Chun, Youngkweon Lee, Kyu-Sam Han, Hyun Kyum Kim, Tae Hee Park and Hyun Je Park
J. Mar. Sci. Eng. 2025, 13(8), 1514; https://doi.org/10.3390/jmse13081514 - 6 Aug 2025
Abstract
In this study, we applied a new technique for vegetatively transplanting kelp Eisenia bicyclis to restore macroalgal habitats. We aimed to assess the restoration success of the E. bicyclis bed by comparing the carbon and nitrogen stable isotope ratios of macrobenthic consumers and [...] Read more.
In this study, we applied a new technique for vegetatively transplanting kelp Eisenia bicyclis to restore macroalgal habitats. We aimed to assess the restoration success of the E. bicyclis bed by comparing the carbon and nitrogen stable isotope ratios of macrobenthic consumers and their isotopic niches in artificial and control (barren ground) habitats. Except for the deposit feeding group, no significant differences were observed in isotopic values of the other feeding groups (suspension feeders, herbivores, omnivores, and carnivores) between the two sites. In contrast, our results showed wider isotopic niche indices for all feeding groups at the transplantation site compared to those at the control site, suggesting increased trophic diversity in the artificial habitat. Overall, these results indicate that the macroalgal bed created using the new method can play an ecological role in restoring functional properties of food web structures via trophic support of degraded coastal ecosystems. Full article
(This article belongs to the Section Ocean Engineering)
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10 pages, 223 KiB  
Article
Lipoprotein(a) Levels in Heart Failure with Reduced and Preserved Ejection Fraction: A Retrospective Analysis
by Alaukika Agarwal, Rubab Sohail and Supreeti Behuria
Hearts 2025, 6(3), 20; https://doi.org/10.3390/hearts6030020 - 6 Aug 2025
Abstract
Background/Objectives: While elevated Lp(a) levels are associated with incident heart failure development, the role of Lp(a) in established heart failure with reduced ejection fraction (HFrEF) versus heart failure with preserved ejection fraction (HFpEF) remains unexplored. Methods: We conducted a retrospective analysis of 387 [...] Read more.
Background/Objectives: While elevated Lp(a) levels are associated with incident heart failure development, the role of Lp(a) in established heart failure with reduced ejection fraction (HFrEF) versus heart failure with preserved ejection fraction (HFpEF) remains unexplored. Methods: We conducted a retrospective analysis of 387 heart failure patients from our institutional database (January 2018–June 2024). Patients were categorized as HFrEF (n = 201) or HFpEF (n = 186) using ICD-10 codes. Categorical variables were compared between heart failure types using the Chi-square test or Fisher’s Exact test, and continuous variables were compared using the two-sample t-test or Wilcoxon rank-sum test, as appropriate. Logistic regression was utilized to assess heart failure type as a function of Lp(a) levels, adjusting for covariates. Spearman correlation assessed relationships between Lp(a) and pro-BNP levels. Results: Despite significant demographic and clinical differences between HFrEF and HFpEF patients, Lp(a) concentrations showed no significant variation between groups. Median Lp(a) levels were 60.9 nmol/dL (IQR: 21.9–136.7) in HFrEF versus 45.0 nmol/dL (IQR: 20.1–109.9) in HFpEF (p = 0.19). After adjusting for demographic and clinical covariates, Lp(a) showed no association with heart failure subtype (OR: 1.001, 95% CI: 0.99–1.004; p = 0.59). Conclusions: Lp(a) levels do not differ significantly between HFrEF and HFpEF phenotypes, suggesting possible shared pathophysiological mechanisms rather than phenotype-specific biomarker properties. These preliminary findings may support unified screening and treatment strategies for elevated Lp(a) across heart failure, pending confirmation in larger studies. Full article
14 pages, 7789 KiB  
Article
Integrated Sampling Approaches Enhance Assessment of Saproxylic Beetle Biodiversity in a Mediterranean Forest Ecosystem (Sila National Park, Italy)
by Federica Mendicino, Francesco Carlomagno, Domenico Bonelli, Erica Di Biase, Federica Fumo and Teresa Bonacci
Insects 2025, 16(8), 812; https://doi.org/10.3390/insects16080812 - 6 Aug 2025
Abstract
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase [...] Read more.
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase the detection of species with varying ecological traits. We evaluated the effectiveness of integrative sampling methodologies to assess saproxylic beetle diversity within Sila National Park, a Mediterranean forest ecosystem of high conservation value, specifically in two beech forests and four pine forests. The sampling methods tested included Pan Traps (PaTs), Malaise Traps (MTs), Pitfall Traps (PTs), Bait Bottle Traps (BBTs), and Visual Census (VC). All specimens were identified to the species level whenever possible, using specialized dichotomous keys and preserved in the Entomological Collection TB, Unical. Various trap types captured a different number of species: the PaT collected 32 species, followed by the PT with 24, the MT with 16, the VC with 7, and the BBT with 5 species. Interestingly, biodiversity analyses conducted using PAST software version 4.17 revealed that PaTs and MTs recorded the highest biodiversity indices. The GLMM analysis, performed using SPSS software 29.0.1.0, demonstrated that various traps attracted different species with different abundances. By combining multiple trapping techniques, we documented a more comprehensive community composition compared to single-method approaches. Moreover, PaTs, MTs, and PTs recorded 20%, 40%, and 33% of the Near Threatened species, respectively. We report new records for Sila National Park, including the LC species Pteryngium crenulatum (Curculionidae) and the NT species Grynocharis oblonga (Trogossitidae). For the first time in Calabria, the LC species Triplax rufipes (Erotylidae) and the NT species Oxypleurus nodieri (Cerambycidae) and Glischrochilus quadrisignatus (Nitidulidae) were collected. Our results emphasize the importance of method diversity in capturing species with distinct ecological requirements and highlight the relevance of saproxylic beetles as indicators of forest health. These findings support the adoption of multi-method sampling protocols in forest biodiversity monitoring and management programs, especially in biodiversity-rich and structurally heterogeneous landscapes. Full article
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13 pages, 285 KiB  
Article
Examining the Association Between Exposure to the #ShesWell Campaign and Black Women’s Conversations with Healthcare Providers About Pre-Exposure Prophylaxis (PrEP)
by Vanessa Boudewyns, Gabriel Madson, Stefanie K. E. Anderson, Hannah Getachew-Smith, Ryan S. Paquin, Sarah E. Sheff, Nivedita L. Bhushan, Revae S. Downey and Jennifer D. Uhrig
Int. J. Environ. Res. Public Health 2025, 22(8), 1224; https://doi.org/10.3390/ijerph22081224 - 6 Aug 2025
Abstract
Low uptake of pre-exposure prophylaxis (PrEP) for HIV prevention among Black women has been partly attributed to barriers related to patient-provider communication. The goal of this paper was to investigate the association between exposure to the #ShesWell campaign and Black women’s communication about [...] Read more.
Low uptake of pre-exposure prophylaxis (PrEP) for HIV prevention among Black women has been partly attributed to barriers related to patient-provider communication. The goal of this paper was to investigate the association between exposure to the #ShesWell campaign and Black women’s communication about PrEP with a healthcare provider (HCP). We conducted a cross-sectional survey of 403 sexually active, Black women after the initial phase of #ShesWell and used multivariable regression models to analyze whether exposure to #ShesWell was associated with talking to an HCP about PrEP or intention to discuss PrEP with an HCP in the future. Approximately 33% of women surveyed reported exposure to #ShesWell. Campaign exposure was significantly associated with talking to an HCP in the past year about PrEP (OR = 4.96, p = 0.001) and intention to discuss PrEP with an HCP in the next six months (B = 0.29, p = 0.038). Stronger beliefs that doctors should initiate sexual health conversations were positively associated with past PrEP conversations (OR = 2.32, p < 0.001) and future intention (B = 0.11, p = 0.029). Greater comfort discussing prevention (B = 0.35, p < 0.001), self-efficacy discussing PrEP (B = 0.29, p = 0.001), and concern about getting HIV (B = 0.51, p < 0.001) were also associated with intention to discuss PrEP with an HCP. Findings highlight the potential for communication campaigns to motivate patient-provider communication about PrEP, addressing a reported barrier to PrEP uptake among Black women. Full article
(This article belongs to the Special Issue Women and Pre-Exposure Prophylaxis for HIV Prevention)
20 pages, 1316 KiB  
Article
Immunocapture RT-qPCR Method for DWV-A Surveillance: Eliminating Hazardous Extraction for Screening Applications
by Krisztina Christmon, Eugene V. Ryabov, James Tauber and Jay D. Evans
Appl. Biosci. 2025, 4(3), 40; https://doi.org/10.3390/applbiosci4030040 - 6 Aug 2025
Abstract
Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread surveillance. We developed an immunocapture RT-qPCR (IC-RT-PCR) method [...] Read more.
Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread surveillance. We developed an immunocapture RT-qPCR (IC-RT-PCR) method for screening DWV-A infections by capturing intact virus particles from bee homogenates using immobilized antibodies. Validation demonstrated strong correlation with TRIzol®-based extraction (r = 0.821), with approximately 6 Ct reduced sensitivity, consistent with other published immunocapture methods. Performance was adequate for moderate–high viral loads, while TRIzol® showed superior detection for low-dose infections. Laboratory-produced reverse transcriptase showed equivalent performance to commercial enzymes, providing cost savings. IC-RT-PCR eliminates hazardous chemicals and offers a streamlined workflow for surveillance screening where the safety and cost benefits outweigh the sensitivity reduction. This method provides a practical alternative for large-scale DWV-A surveillance programs, while TRIzol® remains preferable for low-level detection and diagnostic confirmation. Full article
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