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Keywords = Muriellopsis sp.

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14 pages, 1976 KB  
Article
Influence of Irradiance and Wavelength on the Antioxidant Activity and Carotenoids Accumulation in Muriellopsis sp. Isolated from the Antofagasta Coastal Desert
by Daniela Diaz-MacAdoo, Maria Teresa Mata and Carlos Riquelme
Molecules 2022, 27(8), 2412; https://doi.org/10.3390/molecules27082412 - 8 Apr 2022
Cited by 18 | Viewed by 3644
Abstract
Microalgae are a valuable natural resource for a variety of biocompounds such as carotenoids. The use of different light spectra and irradiance has been considered as a promising option to improve the production of these compounds. The objective of this study was to [...] Read more.
Microalgae are a valuable natural resource for a variety of biocompounds such as carotenoids. The use of different light spectra and irradiance has been considered as a promising option to improve the production of these compounds. The objective of this study was to evaluate the influence of different wavelengths (white, red, and blue) and irradiances (80 and 350 µmol photons/m2/s) on the photosynthetic state, total carotenoids and lutein productivity (HPLC), lipids (Nile red method) and antioxidant activity (DPPH) of the microalgae Muriellopsis sp. (MCH-35). This microalga, which is a potential source of lutein, was isolated from the coastal desert of Antofagasta, Chile, and adapted to grow in seawater. The results indicate that the culture exposed to high-intensity red light showed the highest biomass yield (2.5 g/L) and lutein productivity (>2.0 mg L−1day−1). However, blue light was found to have a stimulating effect on the synthesis of lutein and other carotenoids (>0.8% dry wt). Furthermore, a direct relationship between lipid accumulation and high light intensity was evidenced. Finally, the highest antioxidant activity was observed with high-intensity white light, these values have no direct relationship with lutein productivity. Therefore, the findings of this study could be utilized to obtain biocompounds of interest by altering certain culture conditions during the large-scale cultivation of MCH-35. Full article
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20 pages, 2435 KB  
Article
Effect of Drying Methods on Lutein Content and Recovery by Supercritical Extraction from the Microalga Muriellopsis sp. (MCH35) Cultivated in the Arid North of Chile
by Mari Carmen Ruiz-Domínguez, Paola Marticorena, Claudia Sepúlveda, Francisca Salinas, Pedro Cerezal and Carlos Riquelme
Mar. Drugs 2020, 18(11), 528; https://doi.org/10.3390/md18110528 - 26 Oct 2020
Cited by 18 | Viewed by 3303
Abstract
In this study, we determined the effect of drying on extraction kinetics, yield, and lutein content and recovery of the microalga Muriellopsis sp. (MCH35) using the supercritical fluid extraction (SFE) process. The strain was cultivated in an open-raceways reactor in the presence of [...] Read more.
In this study, we determined the effect of drying on extraction kinetics, yield, and lutein content and recovery of the microalga Muriellopsis sp. (MCH35) using the supercritical fluid extraction (SFE) process. The strain was cultivated in an open-raceways reactor in the presence of seawater culture media and arid outdoor conditions in the north of Chile. Spray-drying (SD) and freeze-drying (FD) techniques were used for dehydrating the microalgal biomass. Extraction experiments were performed by using Box-Behnken designs, and the parameters were studied: pressure (30–50 MPa), temperature (40–70 °C), and co-solvent (0–30% ethanol), with a CO2 flow rate of 3.62 g/min for 60 min. Spline linear model was applied in the central point of the experimental design to obtain an overall extraction curve and to reveal extraction kinetics involved in the SFE process. A significant increase in all variables was observed when the level of ethanol (15–30% v/v) was increased. However, temperature and pressure were non-significant parameters in the SFE process. The FD method showed an increase in lutein content and recovery by 0.3–2.5-fold more than the SD method. Overall, Muriellopsis sp. (MCH35) is a potential candidate for cost-effective lutein production, especially in desert areas and for different biotechnological applications. Full article
(This article belongs to the Special Issue Bioactive Molecules from Extreme Environments II)
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14 pages, 1370 KB  
Article
Metal Removal from Acid Waters by an Endemic Microalga from the Atacama Desert for Water Recovery
by Marcela Martínez, Yanett Leyton, Luis A. Cisternas and Carlos Riquelme
Minerals 2018, 8(9), 378; https://doi.org/10.3390/min8090378 - 31 Aug 2018
Cited by 9 | Viewed by 4464
Abstract
The environmental problems generated by waste from the mining industry in the mineral extraction for business purposes are known worldwide. The aim of this work is to evaluate the microalga Muriellopsis sp. as a potential remover of metallic ions such as copper (Cu [...] Read more.
The environmental problems generated by waste from the mining industry in the mineral extraction for business purposes are known worldwide. The aim of this work is to evaluate the microalga Muriellopsis sp. as a potential remover of metallic ions such as copper (Cu2+), zinc (Zn2+) and iron (Fe2+), pollutants of acid mine drainage (AMD) type waters. For this, the removal of these ions was verified in artificial acid waters with high concentrations of the ions under examination. Furthermore, the removal was evaluated in waters obtained from areas contaminated by mining waste. The results showed that Muriellopsis sp. removed metals in waters with high concentrations after 4–12 h and showed tolerance to pH between 3 and 5. These results allow proposing this species as a potential bioremediator for areas contaminated by mining activity. In this work, some potential alternatives for application in damaged areas are proposed as a decontamination plan and future prevention. Full article
(This article belongs to the Special Issue Acid Mine Drainage Recovery)
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