Search Results (698)

Apples are one of the most popular fruits in Germany, valued for their regional availability and health benefits. When deciding which apple to buy, several characteristics are important to consumers, including the taxonomic variety, organic cultivation and regional production. To verify that these characteristics are correctly declared, powerful analytical methods are required. In this study, ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-ToF-MS) is applied in combination with random forest to 193 apple samples for the analysis of various authentication issues. Accuracies of 93.3, 85.5, 85.6 and 90% were achieved for distinguishing between German and non-German, North and South German, organic and conventional apples and for six different taxonomic varieties. Since the classification models largely use different parts of the data, which is shown by variable selection, this method is very well suited to answer different authentication issues with one analytical approach. Full article

The impact of five different nets—conventional black, grey, white, and photoselective red and yellow—on the performance of “Gala Redlum” apples was evaluated over a five-year period (2020–2024) and compared to an uncovered control. The cumulative production over this period, ranked from highest to lowest, was as follows: white net (182.4 t/ha), grey net (178.5 t/ha), yellow net (175.8 t/ha), black net (175.5 t/ha), red net (169.5 t/ha), and uncovered control (138.8 t/ha). Vegetative growth results were inconsistent among the studied years. The cumulative photosynthetic rate (An) was slightly higher under the white net (57.9 µmol m⁻² s⁻¹). Fv/Fm values remained closest to optimal levels under the black and grey nets. Netting effectively protected fruits from elevated temperatures, particularly under the grey net, and reduced sunburn damage, with the grey, black, and yellow nets performing best in this regard. Overall profitability was increased by netting: the black net provided the highest cumulative income per hectare over a five-year period (EUR 72,315) alongside the second-lowest sunburn loss (0.69%), while the yellow net also showed strong economic performance (€64,742) with a moderate sunburn loss (1.26%) compared to the red net. Fruit dry matter and soluble solids content (SSC) were generally higher in the uncovered control, whereas °Hue values tended to be higher under the red and yellow nets. In summary, the black and yellow nets provided more balanced microclimatic conditions that enhanced tree performance, particularly under heat stress, leading to improved yield and profitability. However, the economic feasibility of each net type should be evaluated in relation to its installation and maintenance costs. Full article

Fe deficiency in apple trees can lead to leaf chlorosis and impede root development, resulting in significant alterations in signaling, metabolism, and genetic functions, which severely restricts fruit yield and quality. It is well established that WRKY transcription factors (TFs) are of vital significance in mediating plant responses to abiotic stress. Real-time quantitative fluorescence (RT-qPCR) analysis displayed that Fe deficiency stress can significantly induce WRKY69 TF gene expression. However, the potential mechanisms by which the WRKY69 gene involved in Fe deficiency stress remains to be investigated. To address this limitations, the WRKY69 gene (MD09G1235100) was successfully isolated from apple rootstock Malus halliana and performed both homologous and heterologous expression analyses in apple calli and tobacco to elucidate its functional role in response to Fe deficiency stress. The findings indicated that transgenic tobacco plants exhibited enhanced growth vigor and reduced chlorosis when subjected to Fe deficiency stress compared to the wild type (WT). Additionally, the apple calli that were overexpressed WRKY69 also exhibited superior growth and quality. Furthermore, the overexpression of the WRKY69 gene enhanced the ability of tobacco to Fe deficiency stress tolerance by stimulating the synthesis of photosynthetic pigments, increasing antioxidant enzyme activity, and facilitating Fe reduction. Additionally, it increased the resistance of apple calli to Fe deficiency stress by enhancing Fe reduction and elevating the activity of antioxidant enzymes. For example, under Fe deficiency stress, the proline (Pro) contents of the overexpression lines (OE-2, OE-5, OE-6) were 26.18 mg·g⁻¹, 26.13 mg·g⁻¹, and 26.27 mg·g⁻¹, respectively, which were 16.98%, 16.76%, and 17.38% higher than the proline content of 22.38 mg·g⁻¹ in the wild-type lines, respectively. To summarize, a functional analysis of tobacco plants and apple calli displayed that WRKY69 TF serves as a positive regulator under Fe deficiency stress, which provides candidate genetic resources for cultivating apple rootstocks or varieties with strong stress (Fe deficiency) resistance. Full article

Droughts are predicted to intensify with climate change, posing a serious threat to global crop production. Increasing drought tolerance in plants requires an understanding of the underlying mechanisms. This study measured the physiological, phytohormonal and transcriptomic responses to drought in two apple rootstocks to identify drought ‘biomarkers’ and investigated whether the application of acibenzolar-S-methyl (ASM) to the roots could enhance drought tolerance. Two potted-plant trials were conducted on dwarfing (M9) and semi-dwarfing (CG202) apple rootstocks. In both trials, the response patterns in the roots and leaves were compared between irrigated and non-irrigated plants over a 14-day period. In trial 2, ASM was applied 14 days before and immediately before withdrawing irrigation. Drought induced significant decreases in transpiration, photosynthesis and stomatal conductance in both trials. This was accompanied by the accumulation of abscisic acid (ABA) metabolites and the upregulation of ABA pathway transcripts (CYP707A1/A2 and NCED3), a decrease in 12-oxophytodienoic acid (cis-OPDA) and the downregulation of ABA receptor genes (PYL4). The responses to drought were greater in the roots than the leaves, broadly similar across both rootstocks, but differed in strength and timing between the rootstocks. The application of ASM to the roots did not significantly affect the responsiveness to drought in either rootstock. The identified phytohormonal and transcriptomic biomarkers require further validation across a broader range of genotypes. Full article

The increasing interest in fibre-enriched and functional bakery products has led to the exploration of novel plant-based ingredients with both technological functionality and consumer acceptance. This study evaluates the effects of incorporating flours derived from apple (Malus domestica cv. Oberländer Himbeerapfel), sweet chestnut (Castanea sativa), horse chestnut (Aesculus hippocastanum), and red, sessile, and pedunculate oak (Quercus rubra, Q. petraea, and Q. robur) into wheat bread at 5%, 10%, and 15% substitution levels. The impact on crumb structure, crust colour, textural parameters (hardness, adhesiveness, springiness), and sensory attributes was assessed. The inclusion of apple and sweet chestnut flours resulted in a softer crumb, lower adhesiveness, and higher sensory scores related to flavour, aroma, and crust appearance. In contrast, higher levels of oak- and horse-chestnut-derived flours increased crumb hardness and reduced overall acceptability due to bitterness or excessive density. Apple flour preserved crumb brightness and contributed to warm tones, while oak flours caused more intense crust darkening. These findings suggest that selected non-traditional flours, especially apple and sweet chestnut, can enhance the sensory and physical properties of wheat bread, supporting the development of fibre-rich, clean-label formulations aligned with consumer trends in sustainable and functional baking. Full article

Background: The growth of parcel volumes in urban areas, largely driven by e-commerce, has increased the complexity of pickup and delivery operations. To meet demands for cost efficiency, flexibility, and sustainability, CEP (Courier, Express, and Parcel) operators increasingly outsource segments of their last-mile networks. Methods: This study proposes a novel multidimensional cost model for outsourcing, integrating five key variables: transport unit type (parcel/pallet), service phase (pickup/delivery), vehicle category, powertrain type, and delivery point type. The model applies correction coefficients based on internal operational costs, further adjusted for location and service quality using a bonus/malus mechanism. Results: Each cost component is calculated independently, enabling full transparency and route-level cost tracking. A real-world case study was conducted using operational data from a CEP operator in Bosnia and Herzegovina. The model demonstrated improved accuracy and fairness in cost allocation, with measurable savings of up to 7% compared to existing fixed-price models. Conclusions: The proposed model supports data-driven outsourcing decisions, allows tailored cost structuring based on operational realities, and aligns with sustainable last-mile delivery strategies. It offers a scalable and adaptable tool for CEP operators seeking to enhance cost control and service efficiency in complex urban environments. Full article

The integration of HS-SPME-GC/MS and UPLC-MS/MS techniques enabled the profiling of volatile organic compounds (VOCs) and amino acids (AAs) in 18 crabapple flower cultivars, facilitating the development of a novel VOC–AA model. Among the 51 identified VOCs, benzyl alcohol, benzaldehyde, and ethyl benzoate were predominant, categorizing cultivars into fruit-almond, fruit-sweet, and mixed types. The amino acids, namely glutamic acid (Glu), asparagine (Asn), aspartic acid (Asp), serine (Ser), and alanine (Ala) constituted 83.6% of the total AAs identified. Notably, specific amino acids showed positive correlations with key VOCs, suggesting a metabolic regulatory mechanism. The Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) model, when combined with volatile organic compounds (VOCs) and amino acid profiles, enabled more effective aroma type classification, providing a robust foundation for further studies on aroma mechanisms and targeted breeding. Full article

Apples (Malus domestica), one of Iran’s oldest cultivated fruit crops, hold considerable economic importance. In this study, 170 apple leaf samples representing various commercial cultivars were collected across the country. RT-PCR screening targeted five common apple-infecting viruses and two viroids: apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple green crinkle-associated virus (AGCaV), apple mosaic virus (ApMV), apple scar skin viroid (ASSVd), and hop stunt viroid (HSVd). To identify additional or novel agents, 40 RT-PCR-negative samples were pooled into two composite groups and analyzed using next-generation sequencing (NGS). NGS was also performed on individual samples with mixed infections to retrieve full genomes. RT-PCR confirmed the presence of ACLSV, ASPV, ASGV, AGCaV, ApMV, and HSVd. NGS further revealed three additional pathogens: citrus concave gum-associated virus (CCGaV), apple hammerhead viroid (AHVd), and apricot vein clearing-associated virus (AVCaV), which were subsequently detected across the collection by RT-PCR. AGCaV was most prevalent (47.6%), followed by ACLSV (45.8%), HSVd (27.6%), AVCaV (20.5%), ASGV (17%), AHVd (15.2%), ASPV (14.1%), CCGaV (4.7%), and ApMV (3.5%). Mixed infections occurred in 67% of samples. Phylogenetic analysis based on CP genes (ACLSV, ASGV, AGCaV) and full genomes (AVCaV, AHVd) clustered Iranian isolates together, suggesting a common origin. This is the first report in Iran of AGCaV, CCGaV, ApMV, and AVCaV in apple, and notably, the first global report of AVCaV in a non-Prunus host. The findings provide the first comprehensive assessment of the sanitary status of apple trees in Iran. Full article

Although it is one of the most important methods of fruit and vegetable preservation, pickling provides multiple interesting vistas for study, from the variety of the raw vegetal material and the composition of pickling media to the diversity of the microorganisms involved in the process or the quality of the final product. The purpose of this study is to investigate the effects of sodium chloride substitution with potassium or magnesium chloride on the pickling process of apples. Physical (mass, color, texture), chemical (dry matter, acidity, salinity, reducing sugars) and phytochemical parameters of the apples were analyzed during 35 days of fermentation, with a frequency of 7 days. The results show a decrease in dry matter from 14.94 ± 0.25% for all the samples and a continuous increase of lactic acid concentration to a maximum of 0.248 ± 0.032 g lactic acid/100 g product for the magnesium samples. At the same time, the phytochemical profile is enhanced, while the texture becomes softer (a decrease in firmness from 2.53 ± 0.08 N to 0.72 ± 0.02 N was registered for potassium samples). The main conclusion of the study is that sodium chloride could be successfully replaced by potassium or magnesium chloride in the fermentation process of apples. Full article

Kazakhstan is recognized as one of the primary centers of origin of the wild apple Malus sieversii, concentrated mainly in the mountains like Trans-Ile and Zhongar Alatau, as well as parts of the Tarbagatay, Talas Alatau, and Karatau ranges. As the wild progenitor of Malus domestica, M. sieversii harbors a critical genetic diversity essential for apple breeding and conservation efforts. However, its natural populations are increasingly threatened by latent viral infection, which weakens trees, reduces reproduction, and hinders regeneration. In this study, the spread of apple chlorotic leaf spot virus (ACLSV) and apple stem pitting virus (ASPV) was documented in four wild apple populations, with detection rates of 50.2% and 42.2%, respectively. Mixed infections were observed in 28.8% of sampled trees. Apple stem grooving virus (ASGV) was detected exclusively in cultivated orchards, whereas apple mosaic virus (ApMV) and apple necrotic mosaic virus (ApNMV) were not found in either wild forests or cultivated orchards. Using Geographic Information System (GIS) technology, we developed the first spatial distribution maps of these viruses in wild apple forests in the Tian Shan region, revealing site-specific variation and infection rates. These results underscore the importance of monitoring viral infections in wild M. sieversii populations to preserve genetically valuable, virus-free germplasm critical for apple breeding, crop improvement, and sustainable orchard management. Full article

Local apple (Malus x domestica Borkh.) cultivars with tolerance to environmental stress factors must be re-evaluated. While the cultivation of apple trees has a long-standing tradition in Hungary, only a handful of cultivars are produced on a large scale, reflecting a trend in global apple production. The most commonly cultivated apple cultivars worldwide include ‘Golden Delicious’, ‘Red Delicious’, ‘Gala’, ‘Fuji’, and ‘Granny Smith’ (with ‘Jonagold’ and ‘Idared’ being significant in Europe). As a result, genetic diversity among apple cultivars has decreased significantly, which has increased the risk of epidemics if a new pathogen appears. Nonetheless, old and local apple cultivars of the Carpathian Basin have adapted well to Hungarian environmental stress factors and pathogens and seem tolerant to them. In this study, fruit analyses and consumer evaluations of eighteen old, local apple cultivars were conducted. Various physicochemical parameters, such as fruit mass, firmness, total soluble solid content, and total acid content, as well as the content of some biological active compounds, including polyphenol content, antioxidant capacity (FRAP), and pectin content, were determined. Additionally, a consumer evaluation was carried out. Based on the results, most of the old, local apple cultivars exhibit high fruit quality and offer considerable health benefits. The proportion of biologically active compounds in these cultivars is equal to or higher than that in the reference cultivar ‘Watson Jonathan’. Based on the excellent fruit quality and consumer preferences, the ‘Harang alma’ (an attractive fruit exhibiting high FRAP values) and ‘Marosszéki piros’ (with firm flesh and a high pectin content and being particularly tasty) cultivars are recommended for backyard gardens. Full article

Accurate detection of Jinxiu Malus fruits in unstructured orchard environments is hampered by frequent overlap, occlusion, and variable illumination. To address these challenges, we propose YOLOv8-MSP-PD (YOLOv8 with Multi-Scale Pyramid Fusion and Proportional Distance IoU), a lightweight model built on an enhanced YOLOv8 architecture. We replace the backbone with MobileNetV4, incorporating unified inverted bottleneck (UIB) modules and depth-wise separable convolutions for efficient feature extraction. We introduce a spatial pyramid pooling fast cross-stage partial connections (SPPFCSPC) module for multi-scale feature fusion and a modified proportional distance IoU (MPD-IoU) loss to optimize bounding-box regression. Finally, layer-adaptive magnitude pruning (LAMP) combined with knowledge distillation compresses the model while retaining performance. On our custom Jinxiu Malus dataset, YOLOv8-MSP-PD achieves a mean average precision (mAP) of 92.2% (1.6% gain over baseline), reduces floating-point operations (FLOPs) by 59.9%, and shrinks to 2.2 MB. Five-fold cross-validation confirms stability, and comparisons with Faster R-CNN and SSD demonstrate superior accuracy and efficiency. This work offers a practical vision solution for agricultural robots and guidance for lightweight detection in precision agriculture. Full article

The apple tree (Malus domestica), a member of the Rosaceae family, holds significant economic value but faces postharvest challenges, like blue mold caused by Penicillium expansum and gray mold caused by Botrytis cinerea. While synthetic fungicides are widely used, their limitations highlight the need for sustainable alternatives. This study explores the antifungal properties of extracts from Celtis australis, Olea europea var. sylvestris, Chamaerops humilis, and Asparagus albus against these pathogens. In vitro tests assessed mycelial growth inhibition, whereas in vivo trials consisted of measurement of weight loss, firmness, total soluble solids, titratable acidity, and maturity index. Moreover, the phytochemical traits of the extracts were determined using the Folin–Ciocalteu method and HPLC. The results revealed notable antifungal activity, particularly for Celtis australis extract at a concentration of 300 g L⁻¹, which led to significant mycelial growth inhibition (61% for P. expansum and 41% for B. cinerea), a reduction in diseases’ severity (39% and 50%), and a notable decrease in diseases’ incidence (43% and 48%), respectively. Phytochemical analysis reflected the presence of phenols and flavonoids in the tested extracts. Importantly, the natural treatments helped preserve the apples’ quality during storage. Molecular docking studies further revealed that major compounds in Celtis australis extract inhibit the 14α-demethylase enzyme, a key target in fungal sterols biosynthesis. Full article

Lagrein grape (Vitis vinifera L.) pomace and Scilate apple (Malus domestica Borkh.) skin are polyphenol- and antioxidant-rich by-products with promising applications in the food industry. This study investigated the impact of drying and grinding on their antioxidant properties for use in gluten-free baked goods. Regardless of the by-product analysis, the results showed that processing conditions effectively preserved most of the polyphenols. Furthermore, the grape pomace and apple skin flours produced retained approximately 86% and 66% of anthocyanins, respectively. Incorporating these flours into breadsticks, focaccia, and cookies significantly enhanced their polyphenol content (300–727%), anthocyanin content (600–1718%), and antioxidant capacity (280–1200%). The addition of these by-products to baked goods led to a slight decrease in texture and sensory properties. However, adding both grape pomace and apple skin flours significantly improved consumer acceptance compared to products containing only grape pomace flour. This study highlights the potential of upcycling by-products from grapes and apples to enhance the nutritional profile of gluten-free products while supporting a circular economy approach. Full article

This review investigates the potential application of polyphenols extracted from indigenous Malus species as natural stabilizers to enhance ice cream thermodynamic properties. Ice cream quality and stability face significant challenges in regions with unreliable electrical infrastructure, such as Lebanon, where temperature fluctuations compromise product integrity. Polyphenols derived from apple tissues and processing by-products demonstrate promising functionality through interactions with ice cream’s protein and fat components, improving stability, reducing melting rates, and enhancing overall thermodynamic properties. Extraction methodologies are critically evaluated, with emphasis on ultrasound-assisted extraction as an optimal approach balancing efficiency, yield, and the preservation of bioactive compounds. This review provides a comprehensive analysis of polyphenolic profiles across apple varieties and tissues, extraction methodologies, mechanisms of stabilization in frozen desserts, and potential sensory implications. The multifunctional approach addresses both technological challenges in frozen dairy products and evolving consumer preferences for clean-label ingredients while potentially adding nutritional value through the inherent bioactive properties of polyphenols. Furthermore, utilizing apple by-products aligns with circular economy principles, transforming waste streams into value-added ingredients. This approach shows particular promise for regions with cold chain challenges while supporting sustainable agricultural practices. Full article