A halophilic archaeal strain, designated F16-60
T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates. Strain F16-60
T was extremely halophilic (optimum at 30% (
w
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A halophilic archaeal strain, designated F16-60
T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates. Strain F16-60
T was extremely halophilic (optimum at 30% (
w/
v) NaCl) and neutrophilic (optimum pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA and
rpoB´ gene sequences revealed that strain F16-60
T was distinct from species of the related genera
Natronomonas,
Halomarina, and
Halomicrobium, of the order
Halobacteriales. The polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), and one glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The DNA G+C content is 68.0 mol%. The taxonomic study, based on a combination of phylogenetic, genomic, chemotaxonomic, and phenotypic analyses, suggest that strain F16-60
T (= CECT 9635
T = JCM 33318
T), represents a novel species of a new genus within the family
Haloarculaceae and the order
Halobacteriales, for which the name
Haloglomus irregulare gen. nov., sp. nov. is proposed. Metagenomic fragment recruitment analysis revealed the worldwide distribution of members of this genus and suggested the existence of other closely related species to be isolated.
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