Search Results (307)

The Palma Cathedral, a landmark of Mediterranean Gothic architecture, features some of the most structurally daring slender piers in European ecclesiastical design. This study examines the role of marés stone—a local marine calcarenite—in enabling such architectural feats despite its inherent fragility. A multi-technique, non-invasive diagnostic campaign was conducted, including visual inspection, portable microscopy, and infrared thermography, to evaluate the physical condition and behavior of the stone under structural and environmental stress. The results reveal widespread deterioration processes—granular disintegration, alveolization, biological colonization, and structural cracking—exacerbated by the stone’s high porosity and exposure to marine aerosols and thermal fluctuations. Thermographic analysis highlighted moisture retention zones and hidden material discontinuities, while crack monitoring confirmed long-standing, localized structural strain. These findings demonstrate that the Cathedral’s formal audacity was grounded in a refined empirical understanding of marés’ properties. The study underscores the importance of material-based diagnostics for the sustainable conservation of Gothic heritage architecture. Full article

The liturgy of the Catholic Church adopts and reframes passages from Scripture in manifold ways. At times a passage is proclaimed or sung in the liturgy exactly as it appears in the Bible; at other times, a prayer or antiphon draws from one or more verses of the Scripture for inspiration. In order to demonstrate this twofold practice, this article presents a single example of a random day on the liturgical calendar, the Second Sunday of Lent. It will explore the uses of Scripture in the revised entrance and communion antiphons, the restoration of long-neglected ancient presidential prayers, the composition of a new collect and preface, the three-year cycle of readings, and the intersection of biblical references between the Roman Missal and the Liturgy of the Hours. By examining a single example, the reader will come to a deeper appreciation of the depth of the interplay between the Bible and liturgy on every day of the calendar. This article will cite the present and previous Roman Missals, the Roman Gradual, the context for the structure of the Lectionary for Mass on this day as the revisers conceived it, and unique features from the Liturgy of the Hours. It will also show how particular biblical references on one day reappear in other liturgical celebrations, expanding the reader’s appreciation of the specific application of biblical texts to a variety of liturgical events. Full article

The fire at the Notre Dame Cathedral accentuated the sense that this architectural building is a symbol of unity and identity for the population. This symbol extends beyond its French and Christian origins, reflecting the universal nature of cultural heritage, which has the extraordinary ability to unite people across nations, transcending ideologies, religions, and political divisions. The reopening of the Notre Dame Cathedral marks a profound milestone in the preservation of cultural heritage, embodying resilience, restoration, and the enduring significance of shared human history. This research examines the impacts of the restoration of the cathedral at national and global scales through the implementation of questionnaires to visitors to the cathedral’s inauguration. The restoration process, which gained extensive media coverage, not only revived the cathedral’s architectural grandeur but also sparked international discussions about heritage conservation. Through an analysis of the questionnaire results for different factors, this study highlights the complexities of managing a monumental heritage of this magnitude in a globalized world. It reflects the ethical, financial, and social challenges involved in major restoration projects, using Notre Dame as a paradigmatic example. The paper concludes that the reopening of the cathedral is not only a triumph of physical restoration but also a testament to humanity’s resilience in the face of adversity. It serves as a powerful reminder of the importance of safeguarding cultural heritage for future generations while fostering a sense of collective responsibility. Full article

This study presents a digital forensic facial approximation of Wenceslas, Duke of Bohemia (ca. 907–935), based on photographs of the skull attributed to the saint, preserved in St. Vitus Cathedral, Prague. The skull was replicated through structural deformation using public domain images, adjusted to historical measurements, and enriched with anthropometric data. The facial approximation combined traditional techniques with statistical projections from tomography of living individuals, resulting in two versions: an objective one and another with artistic and more speculative elements based on historical descriptions. Comparisons with Petr Parler’s 14th-century statue and a previous reconstruction revealed structural consistency, suggesting the skull may have inspired the sculpture. This work contributes to humanizing historical figures and reinforces Wenceslas’s legacy as a Czech icon. Full article

The Cathedral of Beauvais remains one of the most enigmatic and ambitious architectural undertakings of the Gothic era. Conceived to surpass all other cathedrals in height and grandeur, it achieved unprecedented verticality but collapsed under the weight of its own aspirations. This article reexamines Beauvais as a case study in Gothic engineering—its structural daring, tragic failures, and enduring legacy—through a multidisciplinary approach combining historical research, technical analysis, and philosophical interpretation. Structured in accordance with standard academic conventions, the article outlines its methodology, presents a detailed reconstruction of the cathedral’s history, and discusses its broader cultural and conservation implications. A dedicated discussion section explores not only the engineering and symbolic significance of Beauvais but also the limitations of the present study and potential avenues for future research. Ultimately, the cathedral is framed not merely as a failed monument, but as a lasting testament to the human drive to build beyond known limits. Full article

Background: Bloodstream infections continue to pose a serious global health threat due to their high morbidity and mortality, further worsened by rising antimicrobial resistance and delays in starting targeted therapy. This study assesses the accuracy and timeliness of therapeutic recommendations produced by an artificial intelligence (AI)-driven and machine-learning (ML) clinical decision support system (CDSS), comparing results based on molecular diagnostics alone with those that combine molecular and phenotypic data (standard cultures). Methods: In a prospective cross-sectional study conducted in Lima, Peru, 117 blood cultures were analyzed using FilmArray/GeneXpert for molecular identification and MALDI-TOF/VITEK 2.0 for phenotypic profiling. The AI/ML-based CDSS provided treatment recommendations in two formats, which were assessed for concordance and turnaround time. Results: Therapeutic recommendations showed 80.3% consistency between data types, with 86.3% concordance in pathogen and resistance detection. Notably, molecular-only recommendations were delivered 29 h earlier than those incorporating phenotypic data. Escherichia coli was the most frequently isolated pathogen, with a 95% concordance in suggested therapy. A substantial agreement was observed in treatment consistency (Kappa = 0.80). Conclusions: These findings highlight the potential of using AI-powered CDSS in conjunction with molecular diagnostics to accelerate clinical decision-making in bacteremia, supporting more timely interventions and improved antimicrobial stewardship. Further research is warranted to assess scalability and impact across diverse clinical settings. Full article

Below the central nave of the Gothic Cathedral of Palencia (14th to 16th centuries) lies the Crypt of San Antolín, which represents the remains of a Visigothic building from the mid-7th century. The crypt itself has suffered recurrent episodes of flooding over the centuries. However, the latest flooding, which began in mid-2021 and ended in mid-2023, is one of the most long-lasting episodes on the historical record. To establish the origin of these flooding episodes, the geological and hydrological properties of the subsoil have been determined by direct prospecting techniques (drilling) and indirect geophysical techniques (Electrical Resistivity Tomography). The prospecting has determined that the aquifer in the area has a basin-like geometry, which favors the accumulation of water below the crypt. This work has shown that the recurrent floods suffered by the crypt prior to 2021 are related to episodes of intense rainfall. However, after 2021, there is a direct relationship between the persistent flooding and the onset of landscaping at the Plaza de San Antolín, one of the squares near the cathedral. In addition, previous archaeological excavations carried out in 1965 in that same square had disrupted the stratigraphic column, easing the percolation of water. We conclude that the increase in landscaped areas in archaeological environments may modify the hydrogeological dynamics of the subsoil and affect the surrounding buildings. Full article

This article provides an assessment of the impact of different levels of monovision upon early visual outcomes and quality of vision (QoV) following the bilateral implantation of enhanced monovision intraocular lenses (IOLs). Consecutive patients implanted bilaterally with the Rayone EMV (Rayner) were recruited. The dominant eye was targeted for emmetropia, and myopia was targeted in the nondominant eye. Patients were categorized based upon the postoperative refractive outcome in the nondominant eye as follows: Group A: −0.50 to −1.0 D (n = 40), Group B: <−1.00 = D (n = 46). Uncorrected distance (UDVA), intermediate (UIVA), and near (UNVA) visual acuity, and QoV were compared 3 months postoperatively. Binocular UIVA was 0.05 ± 0.10 and −0.01 ± 0.11logMAR (p = 0.03) in the two respective groups, and binocular UNVA was 0.23 ± 0.09 and 0.14 ± 0.09logMAR (p < 0.001). Day QoV was 8.77 ± 1.33 and 8.13 ± 1.34 for night QoV in group A, and 8.85 ± 0.99 and 7.85 ± 1.35, respectively, in group B. Group A had a lower spectacle independence rate of 55% compared to 89.1%. This IOL provides a satisfactory range of vision with high QoV satisfaction. A postoperative refractive error of −1.0 D or more in the nondominant eye significantly improves binocular UIVA, UNVA, and spectacle independence, without negatively impacting QoV. Full article

Background/Objectives: Microfluidic mixing has become the gold standard procedure for manufacturing nucleic acid lipid-based delivery systems, offering precise control over critical process parameters. The choice and design of microfluidic mixers are often seen as a key driving force affecting the critical quality attributes of the resulting lipid nanoparticles (LNPs). Methods: This study aimed to evaluate LNPs manufactured using two low-cost microfluidic mixers alongside manual mixing (pipette mixing (PM)), followed by characterization studies using orthogonal analytics as well as expression studies to establish whether low-cost microfluidic manufacturing methods are suitable for bench-scale and high-throughput research. Results: The results show that all manufacturing methods can produce LNPs with sizes ranging between 95 and 215 nm with high encapsulation (70–100%), and enhanced analytics showed variations between the LNPs produced using the different mixers. Despite these differences, pipette mixing production of LNPs demonstrated its application as a high-throughput screening tool for LNPs, effectively distinguishing between different formulations and predicting consistent expression patterns both in vitro and in vivo. Conclusions: Overall, these results validate the use of low-cost microfluidic mixers without compromising the efficiency and integrity of the resulting LNPs. This study supports the increased accessibility of small-scale LNP manufacturing and high-throughput screening. Full article

The preservation of historical heritage requires a deep understanding of the interactions between physical and structural phenomena. This study investigates the influence of bells acoustic waves propagation on the dynamic behavior for the historical bell tower of the San Michele Arcangelo Cathedral in Caserta Vecchia, Italy. By combining environmental acoustic measurements with finite element modeling in ABAQUS, the research analyzes vibroacoustic coupling and identifies vibrational modes affected by the bell sounds. Harmonic analysis reveals the relationships between sound frequencies and structural vibrational modes, providing insights into the dynamics of historic towers subjected to repetitive acoustic loads. The findings demonstrate how sound can induce complex structural responses, offering valuable guidance for targeted conservation strategies. This interdisciplinary approach can be extended to other historic buildings, highlighting the critical role of vibroacoustic considerations in safeguarding cultural heritage. Full article

Background/Objectives: Murine models play a key role in guiding formulation and immunogenicity studies across various vaccine platforms, including mRNA-based vaccines. Typically, a narrow age range (6 to 8 weeks) is used in these studies. Here, we investigated whether widening this age range could provide greater flexibility in experimental design without impacting pre-clinical outcomes. Methods: To achieve this, we evaluated two commonly used lipid nanoparticle (LNP) formulations (based on SM102 and ALC-0315 ionizable lipids) containing either firefly luciferase or ovalbumin mRNA in female BALB/c mice aged 4, 8, and 16 weeks. LNPs were prepared and purified via microfluidics, and their size, polydispersity, zeta potential, and encapsulation efficiency were measured. Mice were injected intramuscularly, and the in vivo bioluminescence and antibody titers were measured to evaluate mRNA expression profiles and immunogenicity across the three age groups. Results: Our findings show that the 4-week-old mice exhibited higher protein expression following mRNA administration compared to the older groups; however, no significant differences were observed between the 8- and 16-week-old mice. Despite the initial higher protein expression, the antibody responses after the prime dose were lower in the 4-week-old mice compared to the other two groups. However, following the booster dose, antibody levels were comparable across all three age groups. Conclusions: By identifying a broader age range window, we provide greater flexibility in study design, enhance data comparability across studies, and promote more efficient use of animal resources, all while maintaining reliable and representative results in these murine models. Full article

Background/Objectives: The development of lipid nanoparticles (LNPs) as delivery platforms for nucleic acids has revolutionised possibilities for both therapeutic and vaccine applications. However, emerging studies highlight challenges in achieving reliable in vitro–in vivo correlation (IVIVC), which delays the translation of experimental findings into clinical applications. This study investigates these potential discrepancies by evaluating the physicochemical properties, in vitro efficacy (across three commonly used cell lines), and in vivo performance (mRNA expression and vaccine efficacy) of four LNP formulations. Methods: LNPs composed of DSPC, cholesterol, a PEGylated lipid, and one of four ionizable lipids (SM-102, ALC-0315, MC3, or C12-200) were manufactured using microfluidics. Results: All formulations exhibited comparable physicochemical properties, as expected (size 70–100 nm, low PDI, near-neutral zeta potential, and high mRNA encapsulation). In vitro studies demonstrated variable LNP-mediated mRNA expression in both immortalised and immune cells, with SM-102 inducing significantly higher protein expression (p < 0.05) than the other formulations in immortalised and immune cells. However, in vivo results revealed that ALC-0315 and SM-102-based LNPs achieved significantly (p < 0.05) higher protein expression without a significant difference between them, while MC3- and C12-200-based LNPs exhibited lower expression levels. As vaccine formulations, all LNPs elicited strong immune responses with no significant differences among them. Conclusions: These findings highlight the complexities of correlating in vitro and in vivo outcomes in LNP development and demonstrate the importance of holistic evaluation strategies to optimise their clinical translation. Full article

Human communication has long relied on visual media for interaction, and is facilitated by electronic devices that access visual data. Traditionally, this exchange was unidirectional, constrained to text-based queries. However, advancements in human–computer interaction have introduced technologies like reverse image search and large language models (LLMs), enabling both textual and visual queries. These innovations are particularly valuable in Cultural Heritage applications, such as connecting tourists with point-of-interest recognition systems during city visits. This paper investigates the use of various Vision Language Models (VLMs) for Cultural Heritage visual question aswering, including Bing’s search engine with GPT-4 and open models such as Qwen2-VL and Pixtral. Twenty Italian landmarks were selected for the study, including the Colosseum, Milan Cathedral, and Michelangelo’s David. For each landmark, two images were chosen: one from Wikipedia and another from a scientific database or private collection. These images were input into each VLM with textual queries regarding their content. We studied the quality of the responses in terms of their completeness, assessing the impact of various levels of detail in the queries. Additionally, we explored the effect of language (English vs. Italian) on the models’ ability to provide accurate answers. Our findings indicate that larger models, such as Qwen2-VL and Bing+ChatGPT-4, which are trained on multilingual datasets, perform better in both English and Italian. Iconic landmarks like the Colosseum and Florence’s Duomo are easily recognized, and providing context (e.g., the city) improves identification accuracy. Surprisingly, the Wikimedia dataset did not perform as expected, with varying results across models. Open models like Qwen2-VL, which can run on consumer workstations, showed performance similar to larger models. While the algorithms demonstrated strong results, they also generated occasional hallucinated responses, highlighting the need for ongoing refinement of AI systems for Cultural Heritage applications. Full article

In October 2021, a mural painting was discovered in the crypt of Parma Cathedral (Italy). It was covered by a wall, erasing it form historical memory. The painting, presumably from the 15th–16th century, depicts the Madonna and Child enthroned in the center, between Saints Peter and John. Before the restoration project, investigations were conducted with different techniques to identify the pigments and binders used, the nature of the surface finish and the efflorescence. Micro-Raman spectroscopy detected numerous pigments compatible with the presumed age. An interesting result concerns the presence of crocoite (lead chromate), an unusual and rare pigment for this period. These pigments were confirmed through investigation by means of Scanning Electron Microscopy coupled to Energy-Dispersive X-ray Spectroscopy (SEM-EDS). Fourier Transform Infrared Spectroscopy (FT–IR) and Gas Chromatography/Mass Spectroscopy (GC/MS) were used to identify the binder and the type of wax used in the finish layer. The rather widespread presence of egg, used to spread the paint, allows us to affirm that this mural painting was created as a fresco, with substantial parts a secco. Full article

Leishmaniasis, a complex disease caused by protozoal parasites of the genus Leishmania, presents various clinical forms, particularly a cutaneous clinical form. Treatment is typically performed with pentavalent antimonial and amphotericin B, both of which have severe side effects that hinder patient compliance. This emphasizes the need for the development of new, effective, and safe treatments. In this study, the leishmanicidal activity of the methanolic extract, an alkaloid-enriched fraction and dicentrine, the main alkaloid of the leaves of Ocotea puberula (Lauraceae), a native Brazilian plant traditionally used by the indigenous population to treat skin affections, was investigated in vitro. Additionally, an in vivo study evaluated the efficacy of a topical cream containing 0.5% dicentrine. The in vitro studies demonstrated high activity and selectivity of methanolic extract, alkaloid-enriched fraction, and dicentrine against the promastigote and amastigote forms of Leishmania (Leishmnia) amazonensis and Leishmania (Viannia) braziliensis. The leishmanicidal effect of dicentrine was related to the modulation of macrophage microbicidal activity. A cream containing 0.5% dicentrine showed high stability and, in permeation studies, dicentrine was retained in a skin-mimicking artificial membrane. This cream effectively inhibited the progression of the skin lesion in BALB/c mice infected with L. (L.) amazonensis, together with a reduced parasite number. Thus, dicentrine offers a promising alternative to the treatment of skin leishmaniasis. Full article