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Extraction, Isolation and Determination of Drugs, Metabolites and (Bio)Markers in...
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Special Issue "Extraction, Isolation and Determination of Drugs, Metabolites and (Bio)Markers in Complex Matrices"

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: 31 December 2021.

Special Issue Editors

Dr. Michele Protti
E-Mail Website
SciProfiles
Guest Editor
Research group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
Interests: HPLC; UHPLC; MS, HRMS; automation; biological matrix; miniaturized sample collection and preatreatment; CNS drugs; metabolites; biomarkers
Special Issues and Collections in MDPI journals
Dr. Laura Mercolini
E-Mail Website
SciProfiles
Guest Editor
Research group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
Interests: bioanalysis; liquid chromatography; mass spectrometry; method validation; microsampling; sample treatment; central nervous system drugs; drugs of abuse; doping agents; natural products
Special Issues and Collections in MDPI journals
Dr. Roberto Mandrioli
E-Mail Website
SciProfiles
Guest Editor
Department for Life Quality Studies, Alma Mater Studiorum – Università di Bologna, 47921 Rimini, Italy
Interests: drug analysis; toxicological analysis; HPLC; capillary electrophoresis; method development; microsampling; sample preparation; antioxidants; biological matrices
Special Issues and Collections in MDPI journals

Special Issue Information

Dear Colleagues,

The advent of increasingly performing technologies for instrumental analysis, especially in terms of selectivity and sensitivity, does not exempt bioanalytical researchers from the implementation of effective and adequate sample treatment strategies. In fact, the soundness and reliability of the analytical data is closely linked to the quality and performance of collection and pretreatment procedures, when applied to samples of both biological and nonbiological nature. The plethora of applicative approaches brought about by novel sample treatment technologies offer today the possibility of reaching unmatched results in terms of efficiency, sustainability, automation and miniaturization of all the preanalytical phases, with undeniable advantages for the scientific community engaged in analytical and bioanalytical evaluations in numerous fields of application.

This Special Issue will be focused on the development, validation, and application of original methodologies aimed at the extraction and qualitative–quantitative analysis of active compounds, metabolites, and (bio)markers in complex matrices, thus requiring advanced pretreatment in terms of extraction, enrichment, and purification. Major focus will be given to the development, validation, and implementation of cutting-edge sample treatment strategies coupled to screening assays and to chromatographic and nonchromatographic techniques.

Dr. Michele Protti
Dr. Laura Mercolini
Dr. Roberto Mandrioli
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Sample pretreatment
  • Extraction techniques
  • Instrumental methods
  • Active compounds
  • Metabolites
  • Biomarker analysis
  • Purification
  • Miniaturization
  • Automation
  • Method validation
  • Chromatography
  • Electrophoresis
  • Mass spectrometry
  • Spectroscopy

Published Papers (2 papers)

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Article
Determination of Synthetic Cathinones in Urine and Oral Fluid by Liquid Chromatography High-Resolution Mass Spectrometry and Low-Resolution Mass Spectrometry: A Method Comparison
by
Sergi Pascual-Caro
,
Francesc Borrull
,
Carme Aguilar
and
Marta Calull
Separations 2020, 7(4), 53; https://doi.org/10.3390/separations7040053 - 02 Oct 2020
Viewed by 983
Abstract
Synthetic cathinones have become very popular recreational drugs. Therefore, determining them in biological samples is now a matter of concern. In recent years, different methods that have been developed can determine these drugs at low-concentration levels. In general, liquid chromatography mass spectrometry detection [...] Read more.
Synthetic cathinones have become very popular recreational drugs. Therefore, determining them in biological samples is now a matter of concern. In recent years, different methods that have been developed can determine these drugs at low-concentration levels. In general, liquid chromatography mass spectrometry detection plays an important role in these methods and the trend is to use low-resolution and high-resolution mass spectrometry. In this article, for the first time, we compare these two analyzers using an Orbitrap and a triple quadrupole mass spectrometer in order to determine a group of synthetic cathinones in urine and oral fluid samples. For this comparison, we evaluated and compared different parameters: Method detection and quantification limits, linearity, apparent recoveries, matrix effect, repeatability (intra-day), reproducibility (inter-day), and accuracy. Similar results were obtained for the two analyzers for the apparent recoveries and matrix effect. However, triple quadrupole showed higher sensitivity compared to Orbitrap for both urine and oral fluid samples. The quantification limits in urine and the detection limits in saliva were two times lower for triple quadrupole. Finally, when blind samples were analyzed to study the accuracy, similar results were obtained for both analyzers. Full article
(This article belongs to the Special Issue Extraction, Isolation and Determination of Drugs, Metabolites and (Bio)Markers in Complex Matrices)
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Article
Improved Achiral and Chiral HPLC-UV Analysis of Ruxolitinib in Two Different Drug Formulations
by
Alessandro Di Michele
,
Aurélie Schoubben
,
Ina Varfaj
,
Alessandro D’Arpino
,
Laura Mercolini
,
Roccaldo Sardella
,
Maurizio Ricci
and
Enrico Tiacci
Separations 2020, 7(3), 47; https://doi.org/10.3390/separations7030047 - 07 Sep 2020
Viewed by 1180
Abstract
In this paper, two new reversed-phase (RP) HPLC-UV methods enabling the quantitative achiral and chiral analysis of ruxolitinib in commercial tablets (containing 20 mg of active pharmaceutical ingredient, API) and not commercially available galenic capsules (with 5 mg of API) are described. For [...] Read more.
In this paper, two new reversed-phase (RP) HPLC-UV methods enabling the quantitative achiral and chiral analysis of ruxolitinib in commercial tablets (containing 20 mg of active pharmaceutical ingredient, API) and not commercially available galenic capsules (with 5 mg of API) are described. For the achiral method based on the use of a water/acetonitrile [70:30, v/v; with 0.1% (v) formic acid] eluent, a “research validation” study was performed mostly following the “International Council for Harmonization” guidelines. All the system suitability parameters were within the acceptance criteria: tailing factor, between 1.7 and 2.0; retention factor, 2.2; number of theoretical plates, >9000. The linearity curve showed R2 = 0.99 (Rxv2 = 0.99), while trueness (expressed as recovery) was between 96.3 and 106.3%. Coefficient of variations (CVs) (repeatability: CVw and intermediate precision: CVIP) did not exceed 1.3% and 2.9%, respectively. Moreover, the use of the enantiomeric Whelk-O1 chiral stationary phases operated under similar RP eluent conditions as for the achiral analyses, and the “inverted chirality columns approach (ICCA)” allowed us to establish that the enantiomeric purity of ruxolitinib is retained upon reformulation from tablets to capsules. The two developed methods can allow accurate determinations of ruxolitinib in drug formulations for medical use. Full article
(This article belongs to the Special Issue Extraction, Isolation and Determination of Drugs, Metabolites and (Bio)Markers in Complex Matrices)
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