Special Issue "Advances in Hydrophilic Interaction Chromatography"

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Chromatographic Separations".

Deadline for manuscript submissions: 31 December 2022 | Viewed by 182

Special Issue Editors

Prof. Dr. Alessia Ciogli
E-Mail Website
Guest Editor
Department of Chemistry and Drug Technologies, University of Rome La Sapienza, 00185 Rome, Italy
Interests: synthesis of stationary phases; enantioselective separations; hyphenated HPLC methods; stereostability studies by dynamic HPLC and NMR; heterogeneous organocatalysis
Special Issues, Collections and Topics in MDPI journals
Dr. Giulia Mazzoccanti
E-Mail Website
Guest Editor
Department of Pharmaceutical Chemistry and Technology, "Sapienza" University of Rome, 00185 Rome, Italy
Interests: synthesis of stationary phases; enantioselective separations; hyphenated HPLC methods; chirality; chiral stationary phases (CSPs); ultra-high-performance liquid chromatography (UHPLC); natural products; pharmaceuticals; biopharmaceuticals

Special Issue Information

Dear Colleagues,

Hydrophilic interaction liquid chromatography (HILIC) is a complementary technique to normal-phase (NP) and reversed-phase (RP) chromatography. The HILIC mode is particularly amenable in separating highly hydrophilic and amphiphilic compounds that are too polar to be retained in RP-LC and poorly charged to establish electrostatic interaction in ion-exchange chromatography. Indeed, HILIC has been successfully applied to separate small organic acids, basic drugs, and neutral and charged pharmaceuticals. In particular, exciting applications concern the analysis of carbohydrates and peptides, biological fluids, and studies concerning -omics sciences (i.e., metabolomics, peptidomics, and proteomics).

Specifically designed stationary phases are continuously developed to extend their use in these fields. However, the main drawback is their chemical stability attested by the bleeding effect, making their stability one of the most challenging in developing stationary phases for HILIC.

Due to its potential to solve many complex separation problems, HILIC is increasingly attracting much interest. This Special Issue aims to collect recent trends in the HILIC world spanning from the development of dedicated stationary phases, their characterization and use, to analytical applications in HPLC and UHPLC fields, including hyphenated LC-MS and 2D-LC techniques. In addition, fundamental and theoretical results in the elucidation of the retention mechanism are also welcome.

With this Special Issue, we hope to offer an update to the state of knowledge of hydrophilic interaction chromatography.

Prof. Dr. Alessia Ciogli
Dr. Giulia Mazzoccanti
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • stationary phases
  • basic analytes
  • oligopeptide analysis
  • sugar separations

Published Papers

This special issue is now open for submission.
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