Special Issue "Separations and Analysis of Proteins in Biological Samples"

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Bioanalysis/Clinical Analysis".

Deadline for manuscript submissions: 15 March 2022.

Special Issue Editor

Prof. Dr. Ivan Mikšík
E-Mail Website
Guest Editor
1. Institute of Physiology of the Czech Academy of Sciences, Vídenska 1083, 142 20 Prague 4, Czech Republic
2. Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
Interests: gel electrophoresis; electrophoresis; chromatography; mass spectrometry; sequencing; proteins; HPLC; sample preparation; liquid chromatography; extraction; physiology; analytical chemistry; proteomics; zoology; lipids; metabolomics; LC-MS/MS; peptides; LC-MS; chemical biology; carbohydrate chemistry; amino acids; glycobiology; carbohydrates; capillary electrophoresis; mass spectrometry techniques

Special Issue Information

Dear Colleagues,

Analysis of proteins in biological samples is an increasingly evolving and desirable method for studying organisms, their functions, and development. The development of these analyses is made possible by the development of advanced analytical methods. It mainly includes the use of modern methods of mass spectrometry in conjunction with separation techniques. An integral part of this research is the use of appropriate methods for sample preparation that allow targeted analysis (e.g., biomarkers). Thus, the methods may include both targeted analysis and screening studies. It should be borne in mind that proteins are a remarkably diverse group of substances that can differ significantly in their properties. Therefore, the problem of protein analysis in biological samples is a kind of art—analytical art.

The presented Special Issue should include both well-arranged articles providing an overview of the current development of methods and procedures of analyses in real biological samples, as well as experimental works dealing with new procedures and approaches applied in specific analytical problems. Papers on new techniques suitable for protein analysis are also welcome. Of course, the scope of this volume is not limited to the analysis of “pure” proteins but also includes their modifications, such as glycosylation, glycation, phosphorylation, and many others.

The aim of this Special Issue should be to provide a general overview of modern methods used to analyze proteins in biological samples, but also to outline the current trends in these methods, to acquaint the scientific community with modern procedures and approaches to real proteomic analysis, whether targeted or screening studies.

Prof. Dr. Ivan Mikšík
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • protein analysis
  • sample preparation
  • target analysis
  • glycoprotein
  • peptide analysis
  • mass spectrometry
  • biological sample
  • chromatography

Published Papers (2 papers)

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Review

Review
Hemp Growth Factors and Extraction Methods Effect on Antimicrobial Activity of Hemp Seed Oil: A Systematic Review
Separations 2021, 8(10), 183; https://doi.org/10.3390/separations8100183 - 12 Oct 2021
Viewed by 593
Abstract
The bioactive Hemp Seed Oil (HSO) is becoming very popular in the medical and research fields due to its antimicrobial properties against several diseases caused by bacteria and fungi. However, the effect of hemp-growing factors and extraction methods on the bioactivity of HSO [...] Read more.
The bioactive Hemp Seed Oil (HSO) is becoming very popular in the medical and research fields due to its antimicrobial properties against several diseases caused by bacteria and fungi. However, the effect of hemp-growing factors and extraction methods on the bioactivity of HSO does not receive adequate research attention. Therefore, this review aims to investigate the effect of growth factors and extraction methods on the antimicrobial activity of HSO. Articles were retrieved from Google Scholar and the Scopus database and screened against inclusion and exclusion criteria. The study revealed that HSO prefers warm climates and favorable humidity ranging from 20 to 39 °C and 79–100% per year, respectively, and rainfall of 324 mm daily. The multivariate linear regression shown excellent prediction (R2 = 0.94) with climates upon Zone of Growth Inhibition (ZGI) of Gram-positive bacteria. Temperature is the strongest predictor (p < 0.01) followed by humidity and rainfall (p < 0.05). Furthermore, well-drained loam soil rich in organic matter seems to stimulate the antimicrobial activity of HSO. The major constituents that influence HSO’s antimicrobial ability to Staphylococcus aureus were cannabidiol (CBD), β-caryophyllene, and limonene. The extraction methods showed less influence on the HSO bioactivity. HSO did not show significant antioxidant activity, but Hemp Seed Hull (HSH), Hemp Seed Flour (HSF), and Hydrolyzed Hemp Seed Protein (HPH), expressed promising DPPH scavenging ability. Full article
(This article belongs to the Special Issue Separations and Analysis of Proteins in Biological Samples)
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Review
Recent Advances in Chiral Analysis of Proteins and Peptides
Separations 2021, 8(8), 112; https://doi.org/10.3390/separations8080112 - 29 Jul 2021
Cited by 1 | Viewed by 731
Abstract
Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain d-amino acids contained in proteins play a role in [...] Read more.
Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain d-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with d-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer’s disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of d-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations. Full article
(This article belongs to the Special Issue Separations and Analysis of Proteins in Biological Samples)
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