Special Issue Editor

Dr. Artemis P. Louppis
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Guest Editor

Analytical Department, MC Analysis Center LTD, 2563 Nicosia, Cyprus
Interests: food chemistry; chemical analysis; food discrimination; mass spectroscopy; liquid chromatography; gas chromatography; method validation; inductively coupled plasma; sample preparation; pesticides; contaminants; chemometrics

Special Issue Information

Dear Colleagues,

This Special Issue aims to highlight the new methods being developed for the detection and quantification of various contaminants in food. New contaminants are constantly being discovered and existing methodologies are being improved. LC-MS/MS has become one of the powerful analytical tools for the analysis of contaminants in different samples and been shown to be advantageous during analysis with respect to improved sensitivity and specificity. This issue will help the scientific community to publish new or optimized methodologies for the analysis of various contaminants in food using LC-MS/MS.

Dr. Artemis P. Louppis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

LC-MS/MS
food analysis
contaminants
methodology

Published Papers (2 papers)

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Research

Open AccessArticle

Application of a Validated Method for the Identification and Quantification of Mycotoxins in Wines Using UPLC-MS/MS

Artemis P. Louppis

and

Michalis S. Constantinou

Separations 2022, 9(4), 102; https://doi.org/10.3390/separations9040102 - 15 Apr 2022

Viewed by 549

Abstract

The aim of the present study was to develop a rapid, simple and reliable method for the identification and quantification of six mycotoxins in wine using liquid chromatography with electrospray ionization tandem mass spectrometry. The analytical method was fully validated, and calibration curves were made with correlation coefficients >0.9970. A short analysis time and acceptable extraction efficiency were achieved by a direct extraction method of analytes (ochratoxin A, aflatoxin B1, B2, G1, G2 and Zearalenone) with acetonitrile. LOD values were from 0.03 to 0.27 μg kg⁻¹, and LOQ values were from 0.08 to 0.81 μg kg⁻¹, with recoveries at various values from 77 to 108%. The expanded uncertainty was 5–21% expressed at a coverage level of k = 2, at a confidence level of approximately 95%. The performance criteria of the method were fully met according to European legislation (EC) 401/2006. The method was successfully applied to wine samples from Cyprus. The method was simple, low cost, quick, accurate, and sensitive. Full article

(This article belongs to the Special Issue Applications of Liquid Chromatography – Tandem Mass Spectrometric Technique for the Detection and Quantification of Contaminants in Food Samples)

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Open AccessArticle

Monitoring of Aflatoxin M1 in Various Origins Greek Milk Samples Using Liquid Chromatography Tandem Mass Spectrometry

and

Nikolaos S. Thomaidis

Separations 2022, 9(3), 58; https://doi.org/10.3390/separations9030058 - 24 Feb 2022

Viewed by 645

Abstract

Aflatoxin M1(AFM1), a major metabolite of Aflatoxin B1(AFB1), has been identified as a potential contaminant in dairy products. Because of its possible carcinogenicity, the legislation limits as set by Commission Regulation (EC) No. 1881/2006 are very strict, namely 0.050 μg kg⁻¹ in milk and 0.025 μg kg⁻¹ in infant formulas. To meet these requirements, a sensitive and accurate method was developed, employing liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Ιmmunoaffinity columns (R-Biopharm) were used for sample purification and preconcentration of the analyte of interest. The quantification of AFM1 was conducted using fortified milk samples, while Aflatoxin B2 (AFB2) was used as an internal standard (IS). The method was validated in terms of linearity, precision, trueness, limits of detection and quantification and uncertainty. The performance criteria for the method were evaluated based on European Commission Regulation (EC) No. 401/2006 and its most recent amendment, as well as the suggested criteria for revision by the EU Reference Laboratory for Mycotoxins and Plant Toxins. The recovery was in the range of 77.9–81.0% for all fortification levels (0.025–0.050–0.075 μg kg⁻¹), with RSD_R values (Relative Standard Deviation of intermediate precision) ranging from 6.1% to 12%. The method’s detection and quantification limits were 0.0027 μg kg⁻¹ and 0.0089 μg kg⁻¹, respectively. The occurrence of AFM1 was investigated in 40 samples of different animal origin (cow, goat and sheep milk) provided by Greek producers. Full article

(This article belongs to the Special Issue Applications of Liquid Chromatography – Tandem Mass Spectrometric Technique for the Detection and Quantification of Contaminants in Food Samples)

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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title： Application of a validated method for identification and quantification of mycotoxins in wines using UPLC-MS/MS

Authors: Artemis P Louppis and Michalis S Constantinou.

Affiliation: University of Ioannina

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