Special Issue "Advanced 3D Cell Culture Techniques in Micro-Bioreactors"
Deadline for manuscript submissions: 31 August 2021.
Interests: stem cells; artificial stem cell niches; leukemic and hematopoietic stem cells; 3D-co-culture systems; micro-bioreactor development
Interests: 3D cell culture; micro-bioreactors; cell-biomaterial interactions; cell biology-inspired bone regeneration; bone cell differentiation
For more than 100 years, cell cultures have been performed in petri dishes and their derivatives, such as culture flasks or multiwell plates. The establishment of such static 2D cultures for cell-based in vitro studies represents a convenient method for screening purposes and basic research, as they allow the complexity of the in vivo situation, which is characterized by a plethora of cell and tissue interactions, to be circumvented. To get one step closer to the in vivo situation, the in vitro toolbox may be extended by organotypic 3D cultures in micro-bioreactors, which enable the generation of more in-vivo-like results with respect to the cellular microenvironment, namely matrix attachment and a pericellular fluid flow environment. Therefore, 3D cultures in micro-bioreactors represent an additional step during the translational process of in vitro information into the field of tissue engineering, biomaterials, and drug development.
With this Special Issue of Processes, we aim to give an overview of the current developments in 3D culture-based micro-bioreactor systems and their corresponding in vitro models, as well as their potential applications. Topics include, but are not limited to, the following:
- Transferring 2D-cultures into 3D;
- Applying an active flow;
- Combining different cell types for advanced 3D co-culture models;
- Miniaturization of cultures, e.g., for organ-on-a-chip devices;
- High-content-/high-throughput-capable micro-bioreactors;
- Micro-bioreactors in basic research;
- Micro-bioreactors as test beds in drug development;
- Micro-bioreactors for personalized medicine applications.
Prof. Dr. Eric Gottwald
Dr. Brigitte Altmann
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- 3D cell culture
- active flow
- stem cells
- drug development
- primary cell culture
- organotypic culture