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Background: Placental angiogenesis is crucial for ensuring placental function and normal fetal development. It has been demonstrated that elevated plasma levels of homoarginine (hArg), an endogenous amino acid, during pregnancy correlate with enhanced vascular endothelial function. However, the effect of hArg in placental angiogenesis during pregnancy is still ambiguous. This study seeks to evaluate the impact of supplemental hArg during the rapid placental growth phase in early pregnancy, a key moment for placental angiogenesis, on ultimate pregnancy outcomes and placental angiogenesis in rats, as well as its potential processes. Methods: This study utilized thirty 8-week-old female Sprague Dawley rats as pregnant animals, which were randomly allocated to control and treatment groups (15 per group) and administered 20 mg/kg of hArg from embryonic day 0.5 (E0.5) to E13. The dams were euthanized on E21. Results: Maternal hArg dietary supplementation positively influenced pregnancy outcomes, resulting in a significant increase in the number of live-born offspring and total placental weight, alongside elevated maternal circulating reproductive hormone levels. Additionally, the upregulation of the amino acid transporter in the placenta of the treatment group established a basis for hArg accumulation in the placenta, hence promoting hArg-specific augmentation of eNOS-mediated NO production. The treated group simultaneously demonstrated an expanded labyrinthine zone, increased blood sinusoids area, enhanced vascular density, and raised levels of proangiogenic factors. Mechanistically, hArg enhanced the expression of proteins linked with the PI3K/AKT signaling pathway. Conclusions: Supplementation with hArg during the placenta’s rapid growth phase enhances placental angiogenesis, ultimately enhancing pregnancy outcomes. This effect may be attributed to the regulation of the PI3K/AKT signaling pathway.

14 November 2025

The experimental design and processing timeline. After estrus synchronization and mating, female rats confirmed to be at embryonic day 0.5 (E0.5) were randomly and evenly separated into two groups. The control group was fed a basal diet throughout the pregnancy, while the treatment group received 20 mg/kg hArg during the rapid placental growth stage (E0.5–E13) and then returned to the basal diet. Both groups of pregnant rats were euthanized at E21 to collect samples for subsequent experiment. Created in BioRender. Yong, F. (2025) https://BioRender.com/rjieagf (accessed on 27 September 2025).

Background/Objectives: The global syndemic of obesity, undernutrition, and climate change highlight the complex health and environmental challenges faced by young adults. These challenges may intensify during the transition to university. As a matter of fact, limited budgets, time constraints, and insufficient culinary skills often lead to unbalanced diets and increased risk of obesity. University cafeterias, serving large numbers of students, represent an ideal setting to promote healthier and more sustainable eating behaviors. The FOOD-HACK Project aimed to design and implement a cafeteria-based intervention using nudging strategies to promote healthier and more sustainable lunch choices among university students. Methods: This pilot study employed a pre–post design with two independent phases in the Polo Cravino cafeteria at the University of Pavia. Food consumption was assessed over 12 non-consecutive days across four weeks. During the intervention, three nudging strategies were implemented: (1) choice architecture, (2) salient labeling, highlighting healthy and sustainable options, and (3) educational prompts. Results: Across both phases, 2400 tray photographs were collected. Post-intervention, the proportion of trays aligned with the Harvard Healthy Eating Plate and EAT-Lancet Planetary Diet models increased, reflecting higher consumption of vegetables and fruit. Legume-based first courses increased; however, legumes did not substantially replace animal proteins as the main protein source, and meat remained predominant in second courses. Reductions in trays containing multiple carbohydrate sources were also observed. Conclusions: The nudging intervention improved overall meal quality, demonstrating that subtle environmental modifications can guide students toward healthier dietary choices, particularly by increasing fruit and vegetable intake. However, the persistent preference for animal proteins highlights the challenge of shifting protein consumption toward more sustainable sources. These findings suggest that nudging can be an effective tool to promote healthier and more balanced eating behaviors in university settings, though complementary strategies may be needed to foster substantial changes in protein choices.

14 November 2025

Timeline of the FOOD-HACK project.

Background/Objectives: Shift work and chronotype influence timing and type of food consumed, yet their combined influence is unclear. This study determined differences between temporal patterns of eating (times of first (FEO), last (LEO), and largest eating occasions (LarEO), duration of eating window (DEW), eating frequency) and nutrient intake of night shift workers on and off shift and the additional influence of chronotype. Methods: Participants (46.6 ± 10.2 years, BMI: 33.9 ± 5.6 kg/m2, male/female: 57/72) completed work/sleep/food diaries, and the Composite Scale of Morningness. Dietary profiles were characterized by day type as follows: morning shift (MS), 1st night shift (1stNS), subsequent night shifts (SNS), 1st day off after night shifts (1stDONS), or other days off (DO). Results: Across day types, there were significant differences in FEO (p < 0.001), LEO (p < 0.001), LarEO (p = 0.025), DEW (p < 0.001), eating frequency (p = 0.003), total energy (p = 0.022), and fibre intake (p < 0.001). Compared to MS, all night shifts had later FEO, LEO, and LarEO; 1stNS had longer DEW and higher fibre but no differences in frequency, energy, and macronutrient intake. Greater morningness was associated with earlier FEO, LEO, LarEO, and lower %energy from fat and saturated fat. Effect of chronotype on temporal eating patterns was not different across day types; there was a significant, positive relationship between morningness and %energy from carbohydrate (%EnergyCHO) on MS (p = 0.004) and 1stDONS (p = 0.040). Conclusions: Dietary habits of night shift workers vary by shift schedule and degree of morningness. Further studies will confirm if shift schedule is more influential than chronotype on shift workers’ dietary habits.

14 November 2025

Flow chart of number of participants screened and enrolled in the SWIFt study, eligible for this study, and contributing participant numbers and days of sleep, work, and food diaries across each day type. MS: morning shift (n = 43 participants contributing 90 days of data); 1stNS: 1st night shift (n = 93 participants contributing 109 days of data); SNS: subsequent night shift (n = 97 participants contributing 204 days of data); 1stDONS: 1st day off after night shift (n = 97 participants contributing 122 days of data); DO: other days off (n = 108 participants contributing 264 days of data). Mean age and BMI of participants included in this analysis were 46.6 ± 10.2 years and 33.9 ± 5.6 kg/m2 (n = 129) and 56% identified as Female. The demographics of participants who contributed data for each shift/day type are shown in Table 1. Work duration on morning shifts were approximately 1.5 h shorter than on night shifts (p &lt; 0.05). Wake windows were significantly different across each of the day types, with wake window around the 1st night shift being the longest (almost 24 h), and wake window on the first day off after night shifts the shortest (~11 h) (Table 1).
  • Brief Report
  • Open Access

Impact of Diet and Maternal Obesity on Human Milk Hyaluronan

  • Christopher Hoover,
  • Karni S. Moshal and
  • Jeffrey V. Eckert
  • + 4 authors

Background: Human milk hyaluronan (HA), a glycosaminoglycan with barrier-protective and immunomodulatory functions, may be influenced by maternal characteristics. The effects of maternal obesity and acute dietary intake on milk HA concentrations remain unclear. Methods: This secondary analysis included 35 lactating mothers (n = 19 normal weight [NW], n = 16 obese [OB]) at 6 weeks postpartum who participated in two separate, but standardized, protocols: (1) Study One, which consisted of hourly milk collections for six hours following a standardized high-fat meal with a sugar-sweetened beverage beginning at 6:00 am, and (2) Study Two, which consisted of daily morning milk collections for seven consecutive days to assess temporal stability (Monday-Sunday). HA concentrations were quantified by an ELISA and analyzed using a mixed-effects and repeated-measures ANOVA. Results: In Study One, postprandial HA concentrations remained stable with no effect of time, BMI, or time × BMI interaction (p > 0.05). In Study Two, HA did not vary significantly by day (p = 0.082) but was higher in OB versus NW mothers (151.9 ± 18.7 vs. 96.5 ± 12.4 ng/mL; p = 0.0396), with the largest difference observed on Day 1 (p = 0.0117). Mean HA values trended upward later in the week (Day 6 and 7), suggesting potential influences of habitual dietary intake or weekend energy patterns. Conclusions: Milk HA concentrations were not altered by acute dietary intake but were consistently higher across multiple days in mothers with obesity. These results indicate that milk HA varies with maternal metabolic status and may also be influenced by habitual dietary patterns, including fluctuations between weekday and weekend intake.

14 November 2025

Milk hyaluronan (HA) concentrations following a dietary challenge. (A) Hourly HA concentrations measured over 6 h after a high-fat meal with a sugar sweetened beverage (n = 35). A modest decline was observed but did not reach significance (p = 0.058; partial η2 = 0.07). (B) HA concentrations stratified by maternal pre-pregnancy BMI (NW, n = 19; OB, n = 16). No significant effect of BMI group (p = 0.77; Cohen’s d = 0.15), time (p = 0.066; partial η2 = 0.07), or BMI × time interaction (p = 0.62; partial η2 = 0.02) was detected. Data are shown as mean ± SEM.

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Nutrition and Growth of Preterm Neonates during Hospitalization
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Nutrition and Growth of Preterm Neonates during Hospitalization

Impact on Childhood Outcomes
Editors: Antonios K. Gounaris, Rozeta Sokou

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Nutrients - ISSN 2072-6643