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Special Issue "Biofilms from Fermentative Perspective: Structures, Functions and Control 2.0"

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A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: 18 December 2023 | Viewed by 4693

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Dr. Nadezhda Sachivkina

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Guest Editor

Department of Microbiology and Virology, Medical Institute, Peoples’ Friendship University of Russia, Moscow, Russia
Interests: biofilms; yeast biotechnology; candida; yeast adhesins; yeast systems biology; nanobiotechnology; optical density; quorum sensing
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Dear Colleagues,

In nature, as a rule, several types of microorganisms coexist in biofilms in the form of a single community. It is shown that in a biofilm, in comparison with pure planktonic cultures of bacteria, their numerous physiological processes occur in a different way, including the production of metabolites and biologically active substances. Microbial biofilms comprises of major classes of macromolecules like nucleic acids, polysaccharides, proteins, enzymes, lipids, humic substances as well as ions. The presence of these components indeed makes them resilient and enables them to survive hostile conditions.

The goal of this Special Issue is to publish both recent innovative research results, as well as review papers on the production of biofilms and fermentative processes of the biofilms. Review and research papers on development of novel enzymes and microbial strains are also of interest. If you would like to contribute a review paper, please contact one of the editors to discuss the topic relevance before submitting the manuscript.

This is 2.0 version of special issue, the original one can be viewed here:

https://www.mdpi.com/journal/fermentation/special_issues/biofilm_ferment

Dr. Nadezhda Sachivkina
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

bacterial biofilms
antibiotic resistance
therapeutic approaches
bacterial and fungi enzymes
yeast protein structural biology
quorum sensing molecules

Published Papers (4 papers)

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Research

Open AccessArticle

The Effects of L-Lysine-α-oxidase Enzyme and Trichoderma harzianum Rifai Culture Liquid on the Formation of Biofilms by Uropathogenic Multiresistant E. coli

and

Fermentation 2023, 9(8), 710; https://doi.org/10.3390/fermentation9080710 - 26 Jul 2023

Viewed by 453

Abstract

In this study, the data of the antagonistic action of the enzyme L-lysine-α-oxidase in relation to clinical isolates of multiresistant uropathogenic Escherichia coli isolated from patients aged 2 to 17 years of various genders with an established diagnosis of infectious urethritis and/or cystitis are presented. According to the results obtained, the top priority drugs for the treatment of infectious urethritis and/or cystitis are FO (Fosfomycin, 200 µg/disc), IMP (Imipenem, 10 mg/disc), and CIP (Ciprofloxacin, 30 µg/disc). It was found that out of 70 clinical isolates, only 36 of them formed biofilms using the plate method, which is equal to 51.4% of the total number of isolates studied. Despite polyresistance, clinical isolates of E. coli have moderate sensitivity to both the homogeneous enzyme and the culture fluid of the producer. The introduction of an enzyme or culture liquid at an early stage of strain cultivation significantly inhibits the formation of biofilms (91–100%). When introduced at later stages of the experiment—24 h and 48 h—inhibition is less pronounced—20–36% and 5–22%, respectively. Full article

(This article belongs to the Special Issue Biofilms from Fermentative Perspective: Structures, Functions and Control 2.0)

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Open AccessArticle

Biofilm Inhibition, Antibacterial and Antiadhesive Properties of a Novel Biosurfactant from Lactobacillus paracasei N2 against Multi-Antibiotics-Resistant Pathogens Isolated from Braised Fish

Hippolyte Tene Mouafo

Alphonse Tegang Sokamte

Linda Manet

Arsene Joseph Manga Mbarga

Sachivkina Nadezdha

Somashekhar Devappa

and

Augustin Mbawala

Fermentation 2023, 9(7), 646; https://doi.org/10.3390/fermentation9070646 - 09 Jul 2023

Viewed by 635

Abstract

This study aimed to assess the antibiotic susceptibility and biofilm formation ability of pathogens isolated from braised fish as well as characterize and evaluate the antibacterial, antiadhesive, and antibiofilm activities of the biosurfactant from Lactobacillus paracasei subsp. tolerans N2 against these pathogens. The susceptibility of six pathogens isolated from braised fish (Escherichia coli EM2, Staphylococcus aureus SA1, Salmonella enteritidis PE1, Pseudomonas aeruginosa CT3, Yersinia enterolitica MH5, Proteus mirabilis MR2, and Klebsiella pneumoniae AG5) to 16 antibiotics revealed multiple resistances with an MAR index greater than 0.3. These pathogens were able to form biofilms with S. aureus SA1, which showed the highest ability. Using biochemical and elemental analyses, FTIR, GC-MS, ¹H NMR and high-resolution mass spectrometry, the biosurfactant was characterized as a novel glycolipoprotein made of two congeners of mass 482.28 and 507.27 m/z, respectively. They showed bactericidal and antiadhesive activities against all pathogens. The biosurfactant inhibited biofilm formation by these pathogens and eradicated mature biofilms. Full article

(This article belongs to the Special Issue Biofilms from Fermentative Perspective: Structures, Functions and Control 2.0)

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Open AccessArticle

Probiotic-Bacteria (Lactobacillus fermentum)-Wrapped Zinc Oxide Nanoparticles: Biosynthesis, Characterization, and Antibacterial Activity

Rajeshkumar Shanmugam

Tharani Munusamy

Santhoshkumar Jayakodi

Khalid A. Al-Ghanim

Marcello Nicoletti

Nadezhda Sachivkina

and

Marimuthu Govindarajan

Fermentation 2023, 9(5), 413; https://doi.org/10.3390/fermentation9050413 - 26 Apr 2023

Viewed by 1234

Abstract

Recently, fabricated nanoparticles (NPs), which can efficiently penetrate biological systems, have found increased usage in the health and hygiene industries. Microbial enzymes and proteins have recently shown their potential to act as reducing agents for the production of NPs, thereby providing an alternative to physical and chemical methods. Not only is this approach efficient and cost-effective, but it also produces a minimal ecological footprint. In this study, zinc oxide nanoparticles (ZnO NPs) were synthesized using probiotic bacteria (Lactobacillus fermentum) as the reducing and capping agent. Several analytical methods, including Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), ultraviolet–visible spectroscopy (UV–Vis), and atomic force microscopy (AFM), were used to analyze the produced ZnO NPs. The SEM analysis confirmed the spherical form of the nanoparticles and estimated their average size to be between 100 and 120 nm. FT-IR analysis verified that the ZnO NPs’ surfaces contained many functional groups. X-ray diffraction examination evidenced that the biogenically produced nanoparticles were crystalline. AFM analysis revealed that the nanoparticles’ size was about 90–100 nm. The maximum absorption peak, determined via a UV–visible spectrophotometer, was 510 nm. The synthesized ZnO NPs’ antimicrobial activity against various bacterial strains was tested, and the highest level of antimicrobial activity was noted against a Vibrio harveyi strain. The maximum concentration, namely, 20 mM of ZnO NPs, showed the highest antimicrobial activity. These observations indicate that the synthesized ZnO NPs possess remarkable antimicrobial potency. This method is an efficient, environmentally friendly, cost-effective approach for producing ZnO NPs that are useful for various biomedical applications. Full article

(This article belongs to the Special Issue Biofilms from Fermentative Perspective: Structures, Functions and Control 2.0)

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Open AccessArticle

Green Synthesis of Copper Oxide Nanoparticles Using Sesbania grandiflora Leaf Extract and Their Evaluation of Anti-Diabetic, Cytotoxic, Anti-Microbial, and Anti-Inflammatory Properties in an In-Vitro Approach

Kanagavalli Ramasubbu

Siddharth Padmanabhan

Khalid A. Al-Ghanim

Marcello Nicoletti

Marimuthu Govindarajan

Nadezhda Sachivkina

and

Vijayarangan Devi Rajeswari

Fermentation 2023, 9(4), 332; https://doi.org/10.3390/fermentation9040332 - 27 Mar 2023

Cited by 3 | Viewed by 1947

Abstract

Green methods of synthesizing nanoparticles are safer than chemical and physical methods, as well as being eco-friendly and cost-efficient. In this study, we use copper oxide nanoparticles (CuO NPs) fabricated with Sesbania grandiflora (Sg) (Hummingbird tree) leaves to test the effectiveness of green synthesizing methods. The attained Sg-CuO NPs physical and optical nature is characterized by UV-Vis spectroscopy Differential Reflectance Spectroscopy (UV-Vis DRS), Fourier Transform Infra-Red spectroscopy (FTIR), X-ray Diffraction spectroscopy (XRD), Scanning Electron Microscope (SEM), and Energy Dispersive X-ray Analysis (EDAX). UV-Vis spectrum for Sg-CuO NPs revealed a peak at 410 nm. SEM images showed the aggregation of needle-shaped particles, at a size of 33 nm. The amylase and glucosidase enzymes were inhibited by the Sg-CuO NPs up to 76.7% and 72.1%, respectively, indicating a possible antihyperglycemic effect. Fabricated Sg-CuO NPs disclosed the excellent inhibition of DPPH-free radicle formation (89.7%) and repressed protein degradation (81.3%). The results showed that Sg-CuO NPs display good anti-bacterial activity against the gram-negative (Escherichia coli and Pseudomonas aeruginosa) and gram-positive (Staphylococcus aureus). Cytotoxicity of the Sg-CuO NPs was determined using anIC₅₀ of 37 μg/mL. Sg-CuO NPs have shown promising anti-diabetic, anti-oxidant, protein degradation-inhibiting, and anti-microbial properties. Our findings have shown that synthesized Sg-CuO NPs have biological activities that may be utilized to treat bacterial infections linked to hyperglycemia. Full article

(This article belongs to the Special Issue Biofilms from Fermentative Perspective: Structures, Functions and Control 2.0)

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