Special Issue "The New Frontiers of Spectroscopy in Clinical Diagnostic Practice"

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Optical Chemical Sensors".

Deadline for manuscript submissions: 16 March 2023 | Viewed by 867

Special Issue Editor

Prof. Dr. Maria Lasalvia
E-Mail Website
Guest Editor
1. Sperimental and Clinical Medicine Department, Faculty of Medicine, University of Foggia, 71122- Foggia, Italy
2. Biomedical Center, Applied Physics Laboratory, Via Napoli 20 c/o OO.RR, 71122 Foggia, Italy
Interests: FTIR spectroscopy; imaging spectroscopy; Raman spectroscopy; ATR-FTIR spectroscopy; image analysis; PCA-LDA; PLS-DA; machine learning

Special Issue Information

Dear Colleagues,

In recent years, the notable advances related to vibrational techniques, and in particular FTIR spectroscopy, have provided very interesting results for medical diagnostics.

Infrared FTIR spectroscopy of cells and tissues is a growing research area in the field of biomedicine. In fact, numerous studies on the spectroscopic characteristics of biological molecules in tissues have made applications of FTIR spectroscopy increasingly important in diagnostic medicine. With the application of FTIR spectroscopy, it is possible to define biochemical changes that occur in healthy cells when they "get sick". All of this occurs due to the analysis of individual cellular biochemical components, such as DNA, RNA, proteins, lipids and carbohydrates. Spectra of biological samples are a superposition of all contributions from each biochemical component, and thus can provide a molecular diagnosis by defining a "biochemical fingerprint" of the genome, proteome and metabolome of cells or tissues, which can be used in different clinical conditions caused by inflammatory lesions following cancer diagnosis. To date, the identification of morphological changes under a light microscope for the diagnosis of a disease depends on the experience of the observer. FTIR spectroscopy offers an interesting alternative for detecting biochemical changes with a relatively inexpensive, non-subjective technique since it does not require tissue/cellular fixation and, more significantly, does not depend on the observer's judgment to detect cellular/molecular changes. Furthermore, it considerably helps to diagnose borderline, indeterminate or false-negative cases as it detects early changes in the molecular vibrational spectra characteristic of cells.

The Special Issue of Chemosensors will focus on the development and practical applications of spectral and optical sensing techniques, such as FTIR and FTIR imaging, Raman microspectroscopy, ATR-FTIR attenuated total reflectance spectroscopy, and near-infrared (NIR) spectroscopy. Original research papers focusing on the development and methodology of spectral detection and experimental verification using these computer-integrated techniques will be greatly appreciated. We also welcome articles on data analysis techniques, such as PCA-LDA and PLS-DA, for the classification of vibrational spectra and on the development of algorithms, such as those based on machine learning. Both literature review articles and original research papers will be published. Literature review articles should provide an up-to-date overview of state-of-the-art advances in these fields and include key findings from other research groups. We welcome and look forward to your participation in this Special Issue.

Prof. Dr. Maria Lasalvia
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Chemosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • FTIR and FTIR-imaging
  • Raman microspectroscopy
  • near-infrared (NIR) spectroscopy
  • cell/tissue and biochemical fingerprint
  • machine learning

Published Papers (1 paper)

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In Vitro Detection of Biochemical Effect in Human CaCo-2 Cell Line after Exposure to a Low Concentration of a Deltamethrin-Based Pesticide
Chemosensors 2022, 10(11), 438; https://doi.org/10.3390/chemosensors10110438 - 24 Oct 2022
Viewed by 685
Pesticide residues are chemicals frequently found in food as contaminants. Pesticides may have adverse health effects, particularly when the digestive tract is concerned, as a consequence of food ingestion. Deltamethrin is a pyrethroid pesticide widely used in various fields, such as agriculture, veterinary [...] Read more.
Pesticide residues are chemicals frequently found in food as contaminants. Pesticides may have adverse health effects, particularly when the digestive tract is concerned, as a consequence of food ingestion. Deltamethrin is a pyrethroid pesticide widely used in various fields, such as agriculture, veterinary and in the household, so the ingestion of a small amount of this chemical may occasionally occur. To assess whether exposure to pesticide residues may have a biological effect at the intestinal level, it is primarily necessary to perform in vitro exposure experiments about cell lines models of the intestinal barrier at low concentrations of the chemical. In the present study, CaCo-2 cells were exposed to different concentrations of a Deltamethrin-based commercial pesticide, which was diluted in the cell medium. An MTT viability test indicated that the cytotoxic concentration value of the pesticide inside 1 mL of medium is between 10−6 and 10−5 mL. However, the analysis of Raman spectra found that biochemical changes occur inside cells exposed to a non-cytotoxic concentration of 10−6 mL of the pesticide inside 1 mL of the medium. Such changes involve mainly an increase in the ratio between the amount of lipid with respect to that of the protein components in the cell cytoplasm. The results obtained by Raman micro-spectroscopy were confirmed by fluorescence images obtained by using a fluorophore staining neutral lipids. Overall, the obtained results suggest that Raman micro-spectroscopy can be successfully used to monitor the cellular modifications due to exposure at low concentrations of pesticides, as those values that can be found inside food are residuals. Full article
(This article belongs to the Special Issue The New Frontiers of Spectroscopy in Clinical Diagnostic Practice)
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