Immunofluorescent Techniques in Animal Stem Cell Research
A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Molecular Biology".
Deadline for manuscript submissions: 31 July 2026 | Viewed by 1179
Special Issue Editors
2. Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Interests: animal biotechnology; animal stem cells; animal gene bank; confocal microscopy; flow cytometry; MACS; FACS; secretome analysis
Interests: animal biotechnology; embryonal and adult stem cells; animal gene bank; confocal microscopy; flow cytometry; ELISA
Special Issue Information
Dear Colleagues,
Stem cells, representing special cell subsets within the living organism, have been studied for several decades in humans and recently in animals. In general, stem cells are undifferentiated cells, which are present in embryonal, fetal, and adult stages of an organism’s life. The uniqueness of stem cells lies mainly in their self-renewal ability and in their capability to differentiate into different cell lines and give rise cells to different tissues. Due to these attributes, stem cells are applied with great promise in regenerative medicine and cell therapies, as well as for different drug studies or disease models. However, prior to the use of stem cells in any application, their origin and purity must be confirmed with a suitable method. In general, each type of stem cell expresses specific biomolecules such as surface CD markers and/or intracellular/transcriptional factors, which can help to identify their origin. In addition, new stem cell biomarkers are still being sought. Moreover, stem cells may have an important immunomodulatory effect on surrounding tissue by secreting different biomolecules, such as cytokines and growth factors or proteins and exosomes, etc. Other biomolecules such as nucleic acids (DNA, RNA, miRNA, etc.) are also of great interest in stem cell studies.
In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: immunofluorescent techniques such as fluorescent and/or confocal microscopy, flow cytometry, etc., used for phenotyping; viability and/or proliferation analyses; drug screening; gene editing; fluorescence-activated cell sorting; secretome analysis via fluorescent protein assay; exosomes analysis; DNA analysis; RNA analysis (cell cycle, miRNA); etc.
We look forward to receiving your contributions.
Dr. Jaromír Vašíček
Dr. Andrea Svoradová
Guest Editors
Manuscript Submission Information
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Keywords
- animal stem cells
- fluorescence/confocal microscopy
- flow cytometry
- fluorescence-activated cell sorting
- fluorescent protein assay
- stem cell markers
- cell therapy
- drug screening
- exosomes
- DNA analysis
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