Analysis of Morphological, Physiological, and Biochemical Traits of Salt Stress Tolerance in Asian Rice Cultivars at Seedling and Early Vegetative Stages

: Rice ( Oryza sativa L.) is a primary energy food for the Asian population. One of the greatest constraints in rice production is soil salinity because rice is very susceptible to salt. Meanwhile, many agricultural lands in Asia are in saline areas. It is important to identify and develop salt-tolerant rice varieties that highly adapt to Asian climates. By combining morphological, physiological, and biochemical assessments for screening the salt tolerance of 116 Asian rice cultivars, we were able to classify them into tolerant, moderate, and sensitive rice cultivars under salinity stress conditions and also understand salt tolerance mechanisms. The rice cultivars that are salt-tolerant include Pokkali from India, TCCP 266 and IR 45427 from the Philippines, and Namyang 7 from Republic of Korea. However, salt-sensitive rice varieties like IR29 and IR58 are from the Philippines, and Daegudo and Guweoldo are from Korea. The salt-tolerant varieties showed signs of tolerance, including a lower percent reduction in germination percentage, root length, root fresh weight, shoot length, plant biomass, and chlorophyll content. In order to maintain the cellular osmotic balance under saline conditions, the salt-tolerant varieties exhibited less membrane damage, a lower Na/K ratio, high proline and sugar accumulation, and lower levels of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). Pokkali from India, TCCP 266 and IR 45427 from the Philippines, and Namyang 7 from Republic of Korea are recommended as valuable germplasm resources for Asian rice breeding programs in saline agricultural areas.


Introduction
Rice (Oryza sativa L.) is one of the major food sources for more than half of the global population [1][2][3][4].One of the constraints in rice production is soil salinity as it greatly affects rice production because rice is very susceptible to salt, especially at the seedling, early vegetative (three-leaf stage), and reproductive stages [5][6][7][8][9].Based on history, Mesopotamian civilization (now part of Iraq) collapsed due to salinization in agricultural areas that caused crop failures [10].Because of human activities and natural phenomena, soil salinity is increasing.Soil salinity stress generally occurs in rice field areas that have improper irrigation and drainage systems and also in coastal areas [11,12].The effects of soil salinity stress also depend on rice genotypes and salt concentration.Indica rice is more salt-tolerant compared with japonica rice subspecies [13].Soil salinity is a serious problem in most of the rice-growing areas of Asian countries in the tropics and temperate regions.Most of the agricultural areas in Asian regions have saline soils [14,15].Rice is the most susceptible crop to soil salinity stress compared with barley and wheat [16].Saline soils have an electrical conductivity (ECe) greater than 4 dSm −1 (~40 mM NaCl) because of the presence of salts, including sodium chloride, bicarbonates, magnesium, and calcium sulfates, and also a number of inorganic ions, such as Na + , Ca 2+ , Mg 2+ , K + , CO 3 2− , HCO 3− , SO 4 2− , and NO 3− [17,18].Sodium chloride (NaCl) is the most abundant salt in saline soils.
Saline soil conditions make it difficult for roots to uptake nutrients and water, which induces osmotic and ionic stress in rice plants [19].Under salinity stress conditions, there is a large amount of Na + influx into plant cells and an increase in the Na + concentrations in the cytoplasm and vacuole, which are toxic to the metabolism mechanism and lead to cell death due to osmotic and ionic stresses [16].Excessive salt concentration in plant cells absolutely leads to reduced cell membrane stability; cell wall damage; cytoplasmic degradation; plasmolysis; endoplasmic reticulum damage; accumulation of malate, citrate, and inositol in the leaf; and increased proline concentration, which leads to a decrease in grain yield [20].A recent study found that Pokkali is a salt-tolerant rice cultivar and IR20 is a salt-sensitive cultivar [21].A salt-tolerant cultivar maintains a higher K + /Na + ratio than a salt-sensitive cultivar.Excess salts in the soil cause high osmotic pressure outside the roots, which reduces the ability of root cells to take up water and nutrients from the soil.In order to adapt to soil salinity stress, plant cells need to accumulate osmolytes, including proline, glycine, taurine, sugars, inositols, glycerol, and sorbitol.A salt-tolerant cultivar accumulates more proline than a salt-sensitive cultivar [22,23].
Salinity stress affects the morphological, physiological, and biochemical characteristics of rice plants, which vary with the growth stages, including reductions in plant height, productive tiller number, biomass, grain yield, filled grain per panicle, grain weight, grain quality, harvest index, and photosynthetic activity and increased the uptake of Na + and Cl − to the shoot [24][25][26].Under salinity stress at 3.5 dSm −1 or 50 mM NaCl, the grain yield of rice was significantly reduced by 90% [27,28].Meanwhile, rice seedlings die at 10 dSm −1 [29].In order to cope with soil salinity stress, rice plants have developed several mechanisms, such as antioxidants for reactive oxygen species (ROS) detoxification, ion homeostasis, biosynthesis and accumulation of osmolytes for osmo-protection, and programmed cell death [22,30].
Soil salinity tolerance is a quantitative trait that is controlled by multiple genes and highly affected by environmental conditions [31][32][33][34][35].The screening of rice for salinity tolerance is also complex.Thus, salinity screening under laboratory conditions is more controllable, rapid, and accurate than field screening.Under field screening, environmental conditions such as dynamic climate factors and soil heterogeneity might influence the accuracy of the determination of the effects of salinity on rice plants [36].The potential indicators for salt tolerance screening for morphological, physiological, and biochemical characteristics are germination percentage, germination time, seedling vigor index, root length, shoot length, plant biomass, cell membrane stability, Na + /K + ratio, proline content, malondialdehyde (MDA) content, hydrogen peroxide (H 2 O 2 ) content, sugar content, ethylene content, and chlorophyll content [37,38].The International Rice Research Institute (IRRI) released a standard evaluation score, ranging from 1 to 9, for visual determination of salinity injury [5].Salt tolerance is indicated by a lower score (1), and salt sensitivity is denoted by a higher score (9), based on the leaf symptoms, tiller number, and growth characteristics under saline conditions.The identification of quantitative trait loci (QTLs) and cloning of genes correlated with salt tolerance in rice may accelerate the development of salt-tolerant rice varieties [39].Meanwhile, not many genes associated with salt tolerance have been isolated and applied in rice breeding programs.
Many QTLs associated with salinity tolerance have been identified by using mapping populations derived from crosses between salt-tolerant rice varieties and salt-sensitive varieties.The most popular marker in QTL mapping is a single-nucleotide polymorphism (SNP) [39,40].A recombinant inbred line (RIL) population derived from a cross between salt-tolerant Pokkali and salt-sensitive IR29 was used to identify a major QTL, Saltol, on chromosome 1 which is involved in regulating Na + /K + homeostasis [41].Other QTLs that have been identified are qSKC-1, qSNC-7, qSE3, and qST1, which play important roles in salt tolerance at different growth stages [22].Genome-wide association studies (GWASs) accelerate the breeding of salt-tolerant rice varieties because of the availability of the rice reference genome and next-generation sequencing (NGS) techniques.A GWAS identified more accurate genomic locations associated with the salinity tolerance.A total of twentyone QTLs and two candidate genes correlated with salinity tolerance were identified in a GWAS with 664 rice varieties [42].A GWAS was also conducted to identify salt-tolerant loci during the reproductive stage [43,44].A number of genes associated with grain yield under salinity stress conditions were also identified in a GWAS with 708 rice genotypes [45].
The analysis of genomics, transcriptomics, proteomics, and metabolomics is also important in identifying genes correlated with salinity tolerance in rice.Under salinity stress, there is a series of changes in rice plants, including changes in gene expression, protein content, and metabolite concentrations [46,47].A number of potential genes associated with salinity tolerance can be identified by comparing the transcriptome, proteome, and metabolome characteristics of salt-tolerant rice varieties with those of salt-sensitive varieties under salinity stress versus normal conditions.The most important goal in relieving the soil salinity problem is to identify and develop rice varieties with high tolerance to salinity stress.Rice genotypes with high salinity tolerance can be identified by using effective screening methods and can provide donor alleles for salt tolerance to develop high-salinitytolerance varieties through rice breeding programs.By understanding the mechanism of salinity tolerance in rice plants based on morphological, physiological, biochemical, and genetic effects, the development of rice varieties with high salinity tolerance by genetic engineering techniques can be accelerated [24].Breeding for rice salt tolerance is a major goal for rice breeders in agricultural areas with saline soil conditions to ensure food sustainability.The objective of this research is to screen the salt tolerance of Asian rice cultivars based on morphological, physiological, and biochemical characteristics.

Morphological Responses of Asian Rice Cultivars to Salt Stress at Seedling and Early Vegetative Stages
All of the Asian rice varieties showed normal growth in the control condition.The germination percentage of Asian rice cultivars under a salinity condition of 200 mM NaCl showed a reduction compared with the normal condition because the salt caused retardation in the plumule and radicle length.Under salinity stress conditions, the Asian rice cultivars had a wide range of germination percentages (Figure 1) and visual symptoms of salt injury at the seedling stage ranging from a score of 1 (salt-tolerant) to a score of 9 (salt-susceptible) (Figure 2).Based on the SES of visual salt injury at the seedling stage, 31 rice cultivars were identified as salt-tolerant, 45 cultivars were classified as moderately salt-tolerant, and 40 cultivars were classified as salt-susceptible.The most salt-tolerant were Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea.Meanwhile, the most salt-susceptible rice varieties were IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea.
Stresses 2023, 3, FOR PEER REVIEW 3 accelerate the breeding of salt-tolerant rice varieties because of the availability of the rice reference genome and next-generation sequencing (NGS) techniques.A GWAS identified more accurate genomic locations associated with the salinity tolerance.A total of twentyone QTLs and two candidate genes correlated with salinity tolerance were identified in a GWAS with 664 rice varieties [42].A GWAS was also conducted to identify salt-tolerant loci during the reproductive stage [43,44].A number of genes associated with grain yield under salinity stress conditions were also identified in a GWAS with 708 rice genotypes [45].The analysis of genomics, transcriptomics, proteomics, and metabolomics is also important in identifying genes correlated with salinity tolerance in rice.Under salinity stress, there is a series of changes in rice plants, including changes in gene expression, protein content, and metabolite concentrations [46,47].A number of potential genes associated with salinity tolerance can be identified by comparing the transcriptome, proteome, and metabolome characteristics of salt-tolerant rice varieties with those of salt-sensitive varieties under salinity stress versus normal conditions.The most important goal in relieving the soil salinity problem is to identify and develop rice varieties with high tolerance to salinity stress.Rice genotypes with high salinity tolerance can be identified by using effective screening methods and can provide donor alleles for salt tolerance to develop highsalinity-tolerance varieties through rice breeding programs.By understanding the mechanism of salinity tolerance in rice plants based on morphological, physiological, biochemical, and genetic effects, the development of rice varieties with high salinity tolerance by genetic engineering techniques can be accelerated [24].Breeding for rice salt tolerance is a major goal for rice breeders in agricultural areas with saline soil conditions to ensure food sustainability.The objective of this research is to screen the salt tolerance of Asian rice cultivars based on morphological, physiological, and biochemical characteristics.

Morphological Responses of Asian Rice Cultivars to Salt Stress at Seedling and Early Vegetative Stages
All of the Asian rice varieties showed normal growth in the control condition.The germination percentage of Asian rice cultivars under a salinity condition of 200 mM NaCl showed a reduction compared with the normal condition because the salt caused retardation in the plumule and radicle length.Under salinity stress conditions, the Asian rice cultivars had a wide range of germination percentages (Figure 1) and visual symptoms of salt injury at the seedling stage ranging from a score of 1 (salt-tolerant) to a score of 9 (saltsusceptible) (Figure 2).Based on the SES of visual salt injury at the seedling stage, 31 rice cultivars were identified as salt-tolerant, 45 cultivars were classified as moderately salttolerant, and 40 cultivars were classified as salt-susceptible.The most salt-tolerant were Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea.Meanwhile, the most salt-susceptible rice varieties were IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea.Other morphological responses to salinity stress conditions, including root length, root fresh weight, shoot length, and plant biomass, showed variation among the Asian rice cultivars (Figure 3).In all of the rice cultivars, root length, root fresh weight, shoot length, and plant biomass were reduced under salinity stress conditions, and the reduction was as high as 59%, 51%, 61%, and 55%, respectively.Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea showed the lowest reduction for root length, root fresh weight, shoot length, and plant biomass.Meanwhile, IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea had the highest reduction for root length, root fresh weight, shoot length, and plant biomass.All of the Asian rice cultivars displayed a root length reduction ranging from 33% to 59%, the reduction for root fresh weight ranged from 35% to 51%, the shoot length had a reduction ranging from 37% to 61%, and the reduction for plant biomass ranged from 31 to 55%.Other morphological responses to salinity stress conditions, including root length, root fresh weight, shoot length, and plant biomass, showed variation among the Asian rice cultivars (Figure 3).In all of the rice cultivars, root length, root fresh weight, shoot length, and plant biomass were reduced under salinity stress conditions, and the reduction was as high as 59%, 51%, 61%, and 55%, respectively.Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea showed the lowest reduction for root length, root fresh weight, shoot length, and plant biomass.Meanwhile, IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea had the highest reduction for root length, root fresh weight, shoot length, and plant biomass.All of the Asian rice cultivars displayed a root length reduction ranging from 33% to 59%, the reduction for root fresh weight ranged from 35% to 51%, the shoot length had a reduction ranging from 37% to 61%, and the reduction for plant biomass ranged from 31 to 55%.Other morphological responses to salinity stress conditions, including root length, root fresh weight, shoot length, and plant biomass, showed variation among the Asian rice cultivars (Figure 3).In all of the rice cultivars, root length, root fresh weight, shoot length, and plant biomass were reduced under salinity stress conditions, and the reduction was as high as 59%, 51%, 61%, and 55%, respectively.Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea showed the lowest reduction for root length, root fresh weight, shoot length, and plant biomass.Meanwhile, IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea had the highest reduction for root length, root fresh weight, shoot length, and plant biomass.All of the Asian rice cultivars displayed a root length reduction ranging from 33% to 59%, the reduction for root fresh weight ranged from 35% to 51%, the shoot length had a reduction ranging from 37% to 61%, and the reduction for plant biomass ranged from 31 to 55%.Rice plants develop leaf symptoms, such as leaf rolling, yellowing, and necrotic lesions, when rice is grown under salinity stress conditions (Figure S1).Leaf rolling leads to a minimization in the water loss by respiration caused by water deficit under salinity stress conditions.Under salinity stress at the early vegetative stage of rice plants with three leaves, leaf rolling, yellowing, necrotic lesions, drying of leaves, and other senescence symptoms were observed in all Asian rice cultivars with a wide range of variation (Figure 4).The damage to leaves under salinity stress conditions is related to the accumulation of Na + from root to shoot.
Rice plants develop leaf symptoms, such as leaf rolling, yellowing, and necrotic lesions, when rice is grown under salinity stress conditions (Figure S1).Leaf rolling leads to a minimization in the water loss by respiration caused by water deficit under salinity stress conditions.Under salinity stress at the early vegetative stage of rice plants with three leaves, leaf rolling, yellowing, necrotic lesions, drying of leaves, and other senescence symptoms were observed in all Asian rice cultivars with a wide range of variation (Figure 4).The damage to leaves under salinity stress conditions is related to the accumulation of Na + from root to shoot.Salinity stress conditions reduced the total chlorophyll content significantly in the Asian rice cultivars classified as salt-susceptible cultivars.The decrease in chlorophyll content in the 116 Asian rice cultivars showed a wide range variation (Figure 4).A reduction in chlorophyll content decreases photosynthetic activity.This result was in line with the results found for other crops, including peas [48], wheat [49], rapeseed [50], and safflower [51].

The Effect of Salinity Stress on Physiological and Biochemical Characteristics of Asian Rice Cultivars at Seedling and Early Vegetative Stages
Under salinity stress conditions, sodium (Na) content in all of the Asian rice cultivars increased significantly.The highest accumulation of sodium was found in the salt-susceptible rice varieties such as IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea followed by moderately salt-tolerant rice varieties including Nipponbare from Japan, Padi Tarab from Malaysia, and Ciherang from Indonesia.Meanwhile, the lowest sodium concentration was observed in the shoots of salt-tolerant varieties such as Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea, but these varieties had higher potassium (K) accumulation than the sensitive ones.The Na/K ratio in all 116 Asian rice varieties exhibited a wide variation (Figure 5).Salinity stress conditions reduced the total chlorophyll content significantly in the Asian rice cultivars classified as salt-susceptible cultivars.The decrease in chlorophyll content in the 116 Asian rice cultivars showed a wide range variation (Figure 4).A reduction in chlorophyll content decreases photosynthetic activity.This result was in line with the results found for other crops, including peas [48], wheat [49], rapeseed [50], and safflower [51].

The Effect of Salinity Stress on Physiological and Biochemical Characteristics of Asian Rice Cultivars at Seedling and Early Vegetative Stages
Under salinity stress conditions, sodium (Na) content in all of the Asian rice cultivars increased significantly.The highest accumulation of sodium was found in the saltsusceptible rice varieties such as IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea followed by moderately salt-tolerant rice varieties including Nipponbare from Japan, Padi Tarab from Malaysia, and Ciherang from Indonesia.Meanwhile, the lowest sodium concentration was observed in the shoots of salt-tolerant varieties such as Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea, but these varieties had higher potassium (K) accumulation than the sensitive ones.The Na/K ratio in all 116 Asian rice varieties exhibited a wide variation (Figure 5).The cell membrane stability of the rice plants under salinity stress was influenced by osmotic adjustment.With the increase in Na + concentration in the cells, the water potential inside of the cells is reduced, which ultimately affects the cell membrane stability.Salt-sensitive rice varieties like IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea accumulated high concentrations of Na + which cause toxicity and cell damage (Figure 6).The cell membrane stability of the rice plants under salinity stress was influenced by osmotic adjustment.With the increase in Na + concentration in the cells, the water potential inside of the cells is reduced, which ultimately affects the cell membrane stability.Saltsensitive rice varieties like IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea accumulated high concentrations of Na + which cause toxicity and cell damage (Figure 6).In order to maintain the cellular osmotic balance under saline conditions, rice plant cells accumulate compatible solutes or metabolites such as proline, malondialdehyde (MDA), hydrogen peroxide (H2O2), and sugar.All of the 116 Asian rice cultivars showed fluctuating levels of compatible solutes under salinity stress conditions (Figure 7).The salt-tolerant rice varieties such as Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea accumulated higher amounts of proline and sugar than the salt-sensitive varieties.Meanwhile, the salttolerant varieties accumulated lower concentrations of MDA and H2O2 compared to the sensitive ones.In order to maintain the cellular osmotic balance under saline conditions, rice plant cells accumulate compatible solutes or metabolites such as proline, malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), and sugar.All of the 116 Asian rice cultivars showed fluctuating levels of compatible solutes under salinity stress conditions (Figure 7).The salt-tolerant rice varieties such as Pokkali from India, TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea accumulated higher amounts of proline and sugar than the salt-sensitive varieties.Meanwhile, the salt-tolerant varieties accumulated lower concentrations of MDA and H 2 O 2 compared to the sensitive ones.

Correlation among Morphological, Physiological, and Biochemical Traits under Salt Stress
The study of the correlation of all of the traits allowed us to understand the relationships among traits under salinity stress tolerance in Asian rice cultivars.Instead of considering only morphological traits such as salinity score, germination percentage, root length, root fresh weight, shoot length, plant biomass, plant height, leaf rolling, and chlorophyll content, physiological and biochemical traits, including the Na/K ratio, cell membrane stability, proline, MDA, hydrogen peroxide, and sugar could be potential parame-

Correlation among Morphological, Physiological, and Biochemical Traits under Salt Stress
The study of the correlation of all of the traits allowed us to understand the relationships among traits under salinity stress tolerance in Asian rice cultivars.Instead of considering only morphological traits such as salinity score, germination percentage, root length, root fresh weight, shoot length, plant biomass, plant height, leaf rolling, and chlorophyll content, physiological and biochemical traits, including the Na/K ratio, cell membrane stability, proline, MDA, hydrogen peroxide, and sugar could be potential parameters for assessing salinity tolerance mechanisms.

Discussion
Seedlings of 116 Asian rice cultivars grown under salinity stress conditions exhibited different visual symptoms of salt injury.Saline conditions decreased the germination percentage of Asian rice cultivars due to the osmotic and ionic stresses causing insufficient water absorption, leading to toxic effects on the seed embryo.Based on the interaction between saline conditions and rice cultivars, among the Asian rice cultivars, there is a wide difference in response to salinity stress conditions.Salt-tolerant seedlings were distinguished from salt-sensitive seedlings under salinity stress conditions.Our results are consistent with previous studies which mentioned that different genotypes of barley, cabbage, and Suaeda species showed different responses to saline conditions with regard to germination percentage [52][53][54][55].Saline conditions decreased the growth of the radicle and plumule.The retardation of the radicle and plumule length was because of the reduction in cell turgor.
Visual salt injury begins with a reduction in effective leaf area.Salt-sensitive rice cultivars had high scores for leaf rolling, which led to a decrease in photosynthetic activity.The distribution for root length, root fresh weight, shoot length, and plant biomass across 116 Asian rice cultivars under salinity stress conditions showed wide fluctuations.Root length and shoot length were shorter in saline conditions compared to the normal condition.The root fresh weight and plant biomass of salt-susceptible cultivars showed a higher percent reduction than salt-tolerant cultivars.Lower percent reductions in root length, root fresh weight, shoot length, and plant biomass were recorded in Pokkali from India, followed by TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea.On the other hand, higher percent reductions in root length, root fresh weight, shoot length, and plant biomass were exhibited by IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea.Salt-tolerant rice cultivars displayed less growth reduction than salt-sensitive cultivars under salinity stress conditions.
The cell membrane stability of 116 Asian rice cultivars was affected by salinity stress conditions.In the salt-sensitive rice cultivars, the cell membrane structure was damaged by salt, which increased the membrane permeability and destroyed the plasma membrane; as a result, the plant growth was reduced [56].In order to maintain the Na/K balance in the shoot, plants absorb more K and exclude the toxic Na [34,57].In this study, salttolerant rice cultivars showed the ability to absorb more K than Na in order to maintain the Na/K balance in the shoot.According to Ponnamperuma [58], the K concentration in the shoot has a positive correlation with salinity tolerance because K is important in stomatal functions.Gregorio and Senadhira [34] also reported that salt-tolerant rice cultivars had a higher K concentration and lower Na content in the shoot.This Na/K balance and the maintenance of a low Na/K ratio are part of the salt tolerance mechanisms and could be promising criteria for the selection of salt-tolerant varieties [59,60].
The variation in chlorophyll content in the 116 Asian rice varieties studied can be used as a potential salinity stress indicator because chlorophyll content is reduced in saltsensitive rice varieties under salinity stress conditions.The salt-sensitive rice varieties, including IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea, showed lower chlorophyll content compared to the salt-tolerant varieties.Salinity stress conditions subject chloroplasts to oxidative stress that reduces the size and number of chloroplasts in the leaves by inhibiting chloroplast synthesis [61][62][63].The activity of the chlorophyllase enzyme, which degrades chlorophyll, increases under salinity stress conditions, leading to a decrease in photosynthetic activity [14].
Salt-tolerant rice varieties accumulated high levels of proline under salinity stress conditions [64].In this study, salt-tolerant varieties such as Pokkali from India followed by TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea also showed higher proline concentrations compared to the salt-sensitive varieties.These results were consistent with those of Ghosh et al. [65], who found that salttolerant rice cultivars like Nonabokra and Pokkali under salinity stress conditions displayed a high proline concentration in the seedling stage.Under salinity stress conditions, proline plays an important role in protecting proteins against denaturation by regulating redox potential and acts as a source of nitrogen and carbon for recovery after salt stress in rice plants [66,67].
Under salinity stress conditions, malondialdehyde (MDA) increased in the salt-sensitive rice cultivars such as IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea.MDA represents biological membrane damage because MDA is the primary product of the decomposition process from unsaturated fatty acids due to oxidative stress under 200 mM NaCl [67][68][69][70][71][72].The amount of hydrogen peroxide (H 2 O 2 ) showed a wide variation in the 116 Asian rice cultivars studied under salinity stress conditions.Salt-tolerant rice varieties like Pokkali from India followed by TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea exhibited lower H 2 O 2 levels than the salt-sensitive ones.The sugar content of salt-tolerant rice varieties showed a significant increase under salinity stress conditions.These results are consistent with a previous study by Chang et al. [73] that found many sugars like raffinose, glucose, fructose, sucrose, galactose, mannose, ribose, xylose, melibiose, galactitol, mannitol, rhamnose, ribose, and erythritol increased in the leaves of salt-tolerant rice cultivars such as Fatmawati and Dendang under salinity stress conditions.Under salinity stress conditions, sugars are accumulated to avoid osmotic stress [74,75].
At the seedling and early vegetative stages, salt-tolerant rice varieties showed higher values for morphological characteristics such as germination percentage, root length, root fresh weight, shoot length, plant biomass, plant height, and chlorophyll content and also higher values for physiological and biochemical traits like cell membrane stability, proline, and sugar content.Meanwhile, salt-sensitive ones had high values for leaf rolling, Na/K ratio, H 2 O 2 , and MDA content.These results were supported by previous studies such as those of Peng et al. [76], Zhang et al. [77], Dwiningsih et al. [78], and Bhowmik et al. [9].Many studies indicated that all of the growth stages in rice plants under salinity stress conditions showed different responses to salt [79].The most sensitive stage to salt is fertilization and flowering, followed by the early vegetative stage, germination, and maturity [80,81].The tolerance responses of rice cultivars to the salinity stress conditions may become the foundation of breeding tolerant rice cultivars in saline agricultural areas.
Since genomic locations that control the abiotic stress tolerance are pleiotropic, the potential candidate genes correlated with salt tolerance also were shown to be associated with heat [82], drought [83], and flooding stresses.Some of the genes that are responsible for salt tolerance were directly linked to heat-responsive pathways, such as germination and seedling development (glycosyl-hydrolase family), the abscisic acid (ABA) signaling pathway (histone-like transcription factors NFYB) [84], and cell wall stability (GAE6) [82].Salt tolerance also showed a correlation with ABA stress response (Asr) genes [84], ERECTAmediated drought tolerance [85], and DREB-based ABA-independent drought tolerance response [86].Each gene that was correlated with salt tolerance was associated with a genotype × environment association, showing that allelic diversity might allow each Asian rice genotype to have diverse adaptation abilities under a salt stress environment [87].
Genome-wide diversity in Asian rice cultivars showed a high genetic variability that allowed them to adapt to various stress conditions, including salinity stress environments.The adaptation of Asian rice cultivars under salt stress conditions is observed in morphological, physiological, and biochemical properties, such as germination percentage; root length; root fresh weight; shoot length; plant biomass; leaf rolling; chlorophyll content; Na/K ratio; cell membrane stability; and proline, malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), and sugar contents.These genome-environment associations are supported by Cortes and Blair [88].
Each gene that correlated with salt tolerance was associated with a genotype × environment association, showing that allelic diversity might allow each Asian rice genotype to have diverse adaptation abilities under a salt stress environment [87].Genome-wide association, genomic prediction, machine learning, big data approaches, and gene editing techniques (such as CRISPR-Cas9) might assist the identification and functional analysis of genes associated with salt tolerance in order to speed up rice adaptability under saline conditions [89][90][91].In order to validate the salt stress mechanisms in Asian rice cultivars, a combination of several abiotic stress conditions, such as drought, heat, and flooding, needs to be completed.And also, more Asian rice cultivars, about 500 cultivars, need to be involved.

Plant Materials
A total of 116 rice genotypes originally from Asian countries (Table 2) were screened for salinity tolerance at the seedling stage and early vegetative stage (3-leaf stage).Seeds were sterilized with 10% (v/v) NaClO for 15 min and washed with distilled water.Pokkali was used as the salt-tolerant standard check, and IR29 was used as the salt-sensitive check [92].The concentration of salt (NaCl) used in this experiment was 200 mM.Ten seeds of each rice genotype were germinated in Petri dishes containing blotting paper and were treated with 200 mM NaCl and kept in an incubator at 30 • C. For the control condition, seeds were germinated with distilled water.After 12 days of salt treatment, transferred to a hydroponic system containing Yoshida's medium [93] with 200 mM NaCl.Rice plants were grown in a growth chamber at a temperature of 28 • C/24 • C for day/night with 65% humidity and a light intensity of 500 µEm −2 s −1 until the early vegetative stage (3-leaf stage).Morphological, physiological, and biochemical responses, including root length, root fresh weight, shoot length, plant biomass, leaf rolling, chlorophyll content, Na + /K + ratio, cell membrane stability, proline content, malondialdehyde (MDA) content, hydrogen peroxide (H 2 O 2 ) content, and sugar content, were measured at early vegetative stage (3-leaf stage).The indicator for the germination of seeds was when the radicle had protruded through the seed coat, the hypocotyl was extended, and the cotyledon was unfolded.The germination percentage and visual symptoms of salt injury at the seedling stage were evaluated based on the standard evaluation score at 12 days of salt treatment (Table 3) [5].Root length was measured as the maximum length of the root for each rice plant at the early vegetative stage (3-leaf stage).The whole weight of the roots of each rice plant was determined as the root fresh weight.Shoot length was measured from the ground surface to the tallest leaf tip with a ruler.The roots and shoot of each rice plant were weighed fresh and determined as plant biomass.

Determination of Leaf Rolling
The leaf rolling score on the three leaves of each plant was identified at the early vegetative stage (3-leaf stage) based on the standard evaluation system for rice [5].The range of the score is from 1 to 5, with 1 indicating unrolled and fully turgid leaves, 2 indicating leaves are folded (deep-V-shaped), 3 indicating leaves are fully cupped (U-shaped), 4 indicating leaf margins touching (O-shaped), and 5 indicating completely rolled leaves.

Analysis of Chlorophyll Content
The chlorophyll content of the fully expanded leaves on the top of each plant was measured by using a Soil and Plant Analyzer Development (SPAD)-502 Plus Chlorophyll Meter (Spectrum Technologies, Aurora, IL, USA).Each leaf was inserted into the sample slot of the SPAD in such a way as to avoid the midrib, and five readings were measured for each leaf.

Measurement of Na/K Ratio
The ratio of sodium (Na) and potassium (K) concentrations in the root and shoot was measured for each rice genotype grown under a salinity condition of 200 mM at the early vegetative stage (3-leaf stage).Each rice plant was rinsed with distilled water and then dried at 65 • C for 2 days.A dried tissue sample for each rice genotype was ground using mortar and pestle.A total of 100 mg of a ground sample was digested in 3 mL of hydrogen peroxide and 5 mL of nitric acid for 3 h at 152-155 • C.Then, the digested sample was diluted to a final volume of 12.5 mL.The concentrations of Na and K were quantified by using a flame photometer (ANA-135, Tokyokoden, Tokyo, Japan).The estimated concentrations of Na and K were calculated based on a standard curve [14].

Measurement of Cell Membrane Stability
The leaf cell membrane stability (CMS) of each rice genotype under saline conditions was determined by using the following equation: CMS (%) = 1 − (E1/E2) × 100 Leaf samples were washed with distilled water and then placed in 10 mL of deionized water at 10 • C for 18 h.Next, the samples were heated at 52 • C for 1 h in a water bath.In order to diffuse the electrolytes from the leaf tissue to aqueous media, the samples were incubated at 10 • C for 24 h.The samples were shaken, and the initial conductance (E1) was measured for each sample.All of the samples were then autoclaved at 121 • C and 0.10 MPa for 15 min in order to kill the plant tissue and release the electrolytes.The samples were placed in an incubator at 25 • C for cooling down; then, the samples were shaken, and the final conductance (E2) was measured [14].

Measurement of Proline Content
A total of 0.5 g of a fresh leaf sample of each rice genotype was diluted in 10 mL of 3% aqueous sulfosalicylic acid and centrifuged for 1 min at 3000 rpm.Then, 2 mL of the supernatant was reacted with 2 mL of glacial acetic acid and 2 mL of ninhydrin acid at 100 • C for 1 h.Exactly 2 mL of toluene was used to extract the chromophore.The absorbance of the chromophore was measured at 520 nm by using a Genesys 10-s UV/Vis Spectrophotometer (Thermo Spectronic, Waltham, MA, USA) with toluene as the blank.A standard curve for proline content was quantified by using purified proline (Sigma Aldrich, Melbourne, VIC, Australia) [14].The proline content of each rice genotype was calculated by using the following formula: ((µg proline/mL × mL toluene)/115.5µg/µmole) × (g sample/5) = µmoles proline gram FW − 1 4.3.8.Measurement of Malondialdehyde (MDA) Content Exactly 0.5 g of rice leaf from each rice genotype was cut and homogenized with 1.5 mL of a 0.5% (w/v) thiobarbituric acid solution consisting of 20% (w/v) trichloroacetic acid and 1.5 mL of distilled water.The solution was heated for 25 min at 95 • C, and then the reaction was stopped by placing the samples on ice.Next, the solution was centrifuged, and the absorbance of the supernatant was measured at 532 and 600 nm.The extinction coefficient of mM −1 cm −1 was used to calculate the MDA content and expressed as nanomoles per gram (nmol/g) of fresh weight [14].4.3.9.Measurement of Hydrogen Peroxide (H 2 O 2 ) Content About 0.1 g of leaf from each rice genotype was diluted in 3 mL of 5% (w/v) trichloroacetic acid and incubated for 3 h at 4 • C. Next, 1 mL of FOX reagent was added to 0.2 mL of the supernatant of the sample and then mixed and incubated for 15 min at 25 • C. The absorbance of the solution was read at 560 nm.The H 2 O 2 content was expressed as micromoles per gram (µmol/g) of fresh weight [14].

Measurement of Sugar Content
A leaf sample from each rice genotype was ground, and 1.0 g of the ground sample was added to 1 mL of distilled water.Then, 1 mL of anthrone reagent was added to the suspension and incubated for 8 min at 25 • C. The absorbance of the solution was read at 630 nm.The content of soluble sugar in each rice genotype was calculated by using a standard graph and expressed in milligrams per gram (mg/g) of fresh weight [14].

Statistical Analysis
The experiment of salinity screening was conducted in a randomized complete block design (RCBD) with five replications.The salinity treatment and control conditions were compared using the least significant difference (LSD) test at a 0.05 probability level.

Conclusions
The combination of morphological, physiological, and biochemical assessments for screening the salt tolerance of 116 Asian rice cultivars enabled the classification of these cultivars into tolerant, moderate, and sensitive rice cultivars under salinity stress conditions and the understanding of salt tolerance mechanisms.The rice cultivars classified as salttolerant included Pokkali from India, followed by TCCP 266 from the Philippines, IR 45427 also from the Philippines, and Namyang 7 from Republic of Korea.Salt-sensitive rice varieties included IR29 from the Philippines, IR58 also from the Philippines, Daegudo from Republic of Korea, and Guweoldo also from Republic of Korea.The identified salttolerant rice varieties may provide a valuable germplasm resource in breeding programs for developing salt-tolerant rice.

Figure 1 .
Figure 1.Germination percentage of 116 Asian rice cultivars under a salinity stress condition of 200 mM NaCl.

Figure 1 .
Figure 1.Germination percentage of 116 Asian rice cultivars under a salinity stress condition of 200 mM NaCl.

Figure 2 .
Figure 2. Standard evaluation score (SES) of visual salt injury at seedling stage of 116 Asian rice cultivars.

Figure 3 .
Figure 3.Effect of salinity stress conditions on root length (A), root fresh weight (B), shoot length (C), and plant biomass (D) of 116 Asian rice cultivars.

Figure 2 .
Figure 2. Standard evaluation score (SES) of visual salt injury at seedling stage of 116 Asian rice cultivars.

Figure 3 .
Figure 3.Effect of salinity stress conditions on root length (A), root fresh weight (B), shoot length (C), and plant biomass (D) of 116 Asian rice cultivars.

Figure 3 .
Figure 3.Effect of salinity stress conditions on root length (A), root fresh weight (B), shoot length (C), and plant biomass (D) of 116 Asian rice cultivars.

Figure 4 .
Figure 4. Leaf symptoms of leaf rolling (left) and chlorophyll content (right) from 116 Asian rice cultivars under salinity stress conditions.

Figure 4 .
Figure 4. Leaf symptoms of leaf rolling (left) and chlorophyll content (right) from 116 Asian rice cultivars under salinity stress conditions.

Figure 5 .
Figure 5. Na/K ratio of 116 Asian rice varieties under salinity stress conditions.

Figure 6 .
Figure 6.Effect of salinity stress conditions on cell membrane stability of 116 Asian rice varieties.

Figure 6 .
Figure 6.Effect of salinity stress conditions on cell membrane stability of 116 Asian rice varieties.

4. 3 .
Measurement of Morphological, Physiological, and Biochemical Traits 4.3.1.Determination of Germination Percentage and Standard Evaluation Score for Salt Injury

Table 1 .
Correlation coefficients among morphological, physiological, and biochemical parameters from 116 Asian rice cultivars under 200 mM NaCl at seedling and early vegetative stages.

Table 2 .
Rice varieties for salt stress tolerance screening.